Receptor Localization

Author : Marjorie Ariano
Publisher : John Wiley & Sons
Page : 276 pages
File Size : 55,6 Mb
Release : 1998-07-16
Category : Medical
ISBN : 0471195243

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Receptor Localization by Marjorie Ariano Pdf

The detection of neurotransmitter receptor locations and distribution densities within the central nervous system and peripheral tissues is receiving intense attention within the neuroscience research community. Neurotransmitter receptors, which receive the chemical signals sent from one neuron to another, are critical links in a highly complex information-processing chain. Pinpointing receptor sites and systems is crucial for understanding neurological function as well as dysfunction. It is also essential for understanding how receptors process information when impacted by such substances as heroin or nicotine, or when affected by neurodegenerative disease. Receptor Localization: Laboratory Methods and Procedures is the first user-friendly guide to the latest techniques and approaches being employed to examine the localization of neurotransmitter receptors in the central nervous system and peripheral tissues. It covers detection methods that are applicable to a wide variety of receptor systems, ranging from genes and ligands to in vito receptors in individuals; and to numerous receptor subtypes, such as nicotine, muscarine, tackykinins, dopamine, adenosine, and GABA. The standard laboratory "recipes" or "tricks" employed in these detection methods are fully discussed, as are the advantages and limitations of each procedure. With contributions from leading experts and extensively illustrated, this book: * Discusses receptor ligand binding methods using irreversible and reversible compounds * Presents antireceptor antisera technology using synthetic peptides and fusion proteins at both the cellular and subcellular resolution levels * Examines molecular assessments of receptors * Describes in situ hybridization, reverse transcriptase-PCR, and fluorescent in situ transcription * Covers new visualization paradigms * Includes physiological analysis of receptor function, cellular detection in the brain slice, and cultured neurons * Discusses the use of PET and SPECT to assess in vivo receptor distributions in animals and humans Receptor Localization: Laboratory Methods and Procedures is an invaluable guide for researchers in the related fields of neurology, biochemistry, and pharmacology. Its lucid descriptions of new detection methods, inclusion of experimental examples, and emphasis on how these experimental approaches are applicable to particular research areas will appeal to both the experienced researcher and novice investigator.

Author : Frances M. Leslie,C. Anthony Altar
Publisher : Unknown
Page : 248 pages
File Size : 46,5 Mb
Release : 1988
Category : Autoradiography
ISBN : UOM:39015015062816

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Receptor Localization by Frances M. Leslie,C. Anthony Altar Pdf

Author : Amitabha Chattopadhyay
Publisher : CRC Press
Page : 230 pages
File Size : 41,5 Mb
Release : 2007-05-17
Category : Medical
ISBN : 9781420005752

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Serotonin Receptors in Neurobiology by Amitabha Chattopadhyay Pdf

A number of developments spanning a multitude of techniques makes this an exciting time for research in serotonin receptors. A comprehensive review of the subject from a multidisciplinary perspective, Serotonin Receptors in Neurobiology is among the first books to include information on serotonin receptor knockout studies. With contributions from l

Author : Antonius M. VanDongen
Publisher : CRC Press
Page : 368 pages
File Size : 47,6 Mb
Release : 2008-10-29
Category : Medical
ISBN : 9781420044157

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Biology of the NMDA Receptor by Antonius M. VanDongen Pdf

The NMDA receptor plays a critical role in the development of the central nervous system and in adult neuroplasticity, learning, and memory. Therefore, it is not surprising that this receptor has been widely studied. However, despite the importance of rhythms for the sustenance of life, this aspect of NMDAR function remains poorly studied. Written

Author : Pertschuk
Publisher : CRC Press
Page : 183 pages
File Size : 45,5 Mb
Release : 2018-02-01
Category : Medical
ISBN : 9781351082600

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Localization Of Putative Steroid Receptors by Pertschuk Pdf

These volumes represent the "state-of-the-art" in morphological methods for detection of steroid receptors. Some physician authors have applied many of the new methodologies in clinical research projects deisgned to improve medical practice, and these attempts have been placed in Volume II. The Background and recent advances presented here are intended to stimulate further experimentation and new innovations, and spur clinical trials by all investigators with an interest in steroid hormones, cell biology, and clinical oncology.

Author : Louis P. Pertschuk
Publisher : CRC Press
Page : 419 pages
File Size : 54,7 Mb
Release : 2019-07-23
Category : Medical
ISBN : 9781351091046

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Localization Of Putative Steroid Receptors by Louis P. Pertschuk Pdf

These volumes represent the "state-of-the-art" in morphological methods for detection of steroid receptors. In Volume I we have attempted to collect current available methods and experimental approahes which might be useful in solving present enigmas. The background and recent advances presented are intended to stimulate further experimentation and new innovations, and spur clinical trials by all investigators with an interest in steroid hormones, cell biology, and clinical oncology.

Author : P. Michael Conn
Publisher : Unknown
Page : 456 pages
File Size : 49,8 Mb
Release : 1993
Category : Science
ISBN : UVA:X002412277

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Receptors by P. Michael Conn Pdf

Receptors initiate the means by which cellular regulators exert their actions on targets. Because of the central role of cell-cell communication and signal transduction, receptors are of intrinsic interest to neuroscientists. Receptor studies utilize both traditional methods of analysis and modern molecular techniques. Key Features * Methods presented for easy adaptation to new systems * Comprehensive protocols included for molecular techniques (PCR, cloning, transfection, coupling); techniques for the determination of receptor subclasses; techniques for localization (in situ hybridization, immunocytochemistry); ligand design (radioactive techniques, biotinylated techniques); receptor-associated kinase * Methodology described for the following receptors: acetylcholine, angiotensin II, bombesin/GRP, dopamine, GABA, G protein-coupled receptors, neurotensin, NGF, NPY, serotonin, somatostatin, tachykinin

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 42,6 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Wolfgang B. Liedtke, MD, PH.D.
Publisher : CRC Press
Page : 502 pages
File Size : 40,5 Mb
Release : 2006-09-29
Category : Medical
ISBN : 9781420005844

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TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades by Wolfgang B. Liedtke, MD, PH.D. Pdf

Since the first TRP ion channel was discovered in Drosophila melanogaster in 1989, the progress made in this area of signaling research has yielded findings that offer the potential to dramatically impact human health and wellness. Involved in gateway activity for all five of our senses, TRP channels have been shown to respond to a wide range of st

Author : Institute of Medicine,Board on Health Sciences Policy,Forum on Neuroscience and Nervous System Disorders
Publisher : National Academies Press
Page : 74 pages
File Size : 51,9 Mb
Release : 2011-08-05
Category : Medical
ISBN : 9780309212212

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Glutamate-Related Biomarkers in Drug Development for Disorders of the Nervous System by Institute of Medicine,Board on Health Sciences Policy,Forum on Neuroscience and Nervous System Disorders Pdf

Glutamate is the most pervasive neurotransmitter in the central nervous system (CNS). Despite this fact, no validated biological markers, or biomarkers, currently exist for measuring glutamate pathology in CNS disorders or injuries. Glutamate dysfunction has been associated with an extensive range of nervous system diseases and disorders. Problems with how the neurotransmitter glutamate functions in the brain have been linked to a wide variety of disorders, including schizophrenia, Alzheimer's, substance abuse, and traumatic brain injury. These conditions are widespread, affecting a large portion of the United States population, and remain difficult to treat. Efforts to understand, treat, and prevent glutamate-related disorders can be aided by the identification of valid biomarkers. The Institute of Medicine's Forum on Neuroscience and Nervous System Disorders held a workshop on June 21-22, 2010, to explore ways to accelerate the development, validation, and implementation of such biomarkers. Glutamate-Related Biomarkers in Drug Development for Disorders of the Nervous System: Workshop Summary investigates promising current and emerging technologies, and outlines strategies to procure resources and tools to advance drug development for associated nervous system disorders. Moreover, this report highlights presentations by expert panelists, and the open panel discussions that occurred during the workshop.

Author : G.F. Gebhart,Robert F. Schmidt
Publisher : Springer
Page : 0 pages
File Size : 43,9 Mb
Release : 2013-11-18
Category : Medical
ISBN : 3642287522

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Encyclopedia of Pain by G.F. Gebhart,Robert F. Schmidt Pdf

The Encyclopedia of Pain includes more than 3,000 entries and provides clear, detailed and up-to-date coverage of the current state of research, and treatment of pain. In addition, detailed essays provide in-depth information on all aspects of nociception and pain, including substrates, causes, pathophysiology, symptoms and signs, diagnoses and treatment. A thousand color figures enhance understanding of this too-little-understood topic. The book is available in print, in online only form, or in a print-online bundle.

Author : James N. C. Kew,Ceri H. Davies
Publisher : Unknown
Page : 586 pages
File Size : 42,8 Mb
Release : 2010
Category : Medical
ISBN : 9780199296750

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Ion Channels by James N. C. Kew,Ceri H. Davies Pdf

Ion channels are intimately involved in the everyday physiological functions that enable us to live a full and varied life. When disease strikes, malfunction of ion channels or their dependent is often involved, either as the cause or the effect of the illness. Thus, billions of dollars have been, and still are being, invested in research to understand the physiological and pathophysiological functions of ion channels in an attempt to develop novel therapeutic treatments for a wide range of diseases. This book provides a comprehensive overview of ion channel structure and function. It comprises two major parts. Part one is an introductory overview of the ion channel superfamily and the generic aspects of ion channel function. This part also reviews the methodologies by which ion channel function can be studied from the perspective of performing detailed biophysical characterization through to the deployment of high throughput approaches for identifying novel ion channel ligands. Part two of the book provides an in-depth review of the individual ion channel subfamilies and, as such, is subdivided into four broad sections: Voltage-Gated Ion Channels, Extracellular Ligand-Gated Ion Channels, Intracellular Ligand-Gated Ion Channels, and Polymodal-Gated Ion Channels, with each chapter focused on specific family members. These chapters have been written by world leading experts and provide a detailed overview of the structure, biophysics, localization, pharmacology, physiology, and disease relevance of each particular ion channel subfamily. Reviewing both the basic principles of ion channel function and providing a detailed up-to-date review of the phsyiological and pharmacological aspects of individual ion channel sub-families, this book constitutes both an excellent introduction to the field for non-specialists, as well as a highly valuable reference text for experienced researchers already working in the ion channel area.

Author : Ewa Rakowicz-Szulczynska
Publisher : CRC Press
Page : 224 pages
File Size : 50,9 Mb
Release : 1993-10-13
Category : Medical
ISBN : 0849347130

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Nuclear Localization of Growth Factors and of Monoclonal Antibodies by Ewa Rakowicz-Szulczynska Pdf

Nuclear Localization of Growth Factors and of Monoclonal Antibodies focuses on how an additional class of chromatin receptors is directly involved in mediation of the action of growth factors on the gene regulation level. The book describes a new intracellular pathway of growth factors and presents methods for identifying nuclear antigens with an epitopic homology of the cell surface antigens. It teaches how to distinguish monoclonal antibodies that penetrate the cell and localize in the nucleus from those that act only on the cell surface. New light is shed on the mechanisms of malignant transformation, and new approaches to developing anti-cancer and anti-HIV drugs are covered. The book will be important for oncologists, biochemists, cell biologists, pharmaceutical researchers, investigative pathologists, neurochemists, and immunologists.

Author : Stefan Bauer,Gunther Hartmann
Publisher : Springer Science & Business Media
Page : 243 pages
File Size : 45,9 Mb
Release : 2007-12-11
Category : Medical
ISBN : 9783540721673

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Toll-Like Receptors (TLRs) and Innate Immunity by Stefan Bauer,Gunther Hartmann Pdf

Overall recent research on TLRs has led to tremendous increase in our understanding of early steps in pathogen recognition and will presumably lead to potent TLR targeting therapeutics in the future. This book reviews and highlights our recent understanding on the function and ligands of TLRs as well as their role in autoimmunity, dendritic cell activation and target structures for therapeutic intervention.

Author : Derek J. Chadwick,Jamie A. Goode
Publisher : John Wiley & Sons
Page : 346 pages
File Size : 42,6 Mb
Release : 2008-04-30
Category : Nature
ISBN : 9780470514917

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P2 Purinoceptors by Derek J. Chadwick,Jamie A. Goode Pdf

Many different pathological conditions are currently under investigation as therapeutic targets of purines including cancer, cardiovascular conditions, behavioural disorders, inflammation, immunoregulation, and neuroendocrine function. This book draws together research on all aspects of P2 purinoceptors and discusses their use in different therapeutic areas.