Receptor Purification

Author : Gerald Litwack
Publisher : Springer Science & Business Media
Page : 498 pages
File Size : 48,5 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461204619

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Receptor Purification by Gerald Litwack Pdf

The purpose of these volumes is to provide a reference work for the methods of purifying many of the receptors we know about. This be comes increasingly important as full-length receptors are overexpressed in bacteria or in insect cell systems. A major problem for abundantly expressed proteins will be their purification. In addition to purification protocols, many other details can be found concerning an individual receptor that may not be available in standard texts or monographs. No book of this type is available as a compendium of purification procedures. Receptor Purification provides protocols for the purification of a wide variety of receptors. These include receptors that bind: neurotransmit ters, polypeptide hormones, steroid hormones, and ligands for related members of the steroid supergene family and others, including receptors involved in bacterial motion. The text of this information is substantial, so as to require its publication in two volumes. Consequently, a division was made by grouping receptors by the nature of their ligands. Thus, in Volume One there are contributions on serotonin receptors, adrenergic receptors, the purification of GTP-binding proteins, opioid receptors, neurotensin receptor, luteinizing hormone receptor, human chorionic gonadotropin receptor, follicle stimulating hormone receptor, thyro tropin receptor, prolactin receptor, epidermal growth factor receptor, platelet derived growth factor receptor, colony stimulating factor recep tor, insulin-like growth factor receptors, insulin receptor, fibronectin receptor, interferon receptor, and the cholecystokinin receptor.

Author : J. Craig Venter,Len C. Harrison
Publisher : Unknown
Page : 192 pages
File Size : 49,9 Mb
Release : 1984
Category : Science
ISBN : UOM:39015007117305

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Receptor Purification Procedures by J. Craig Venter,Len C. Harrison Pdf

Author : Gerald Litwack
Publisher : Humana
Page : 0 pages
File Size : 50,9 Mb
Release : 1990-10-30
Category : Medical
ISBN : 0896031837

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Receptor Purification by Gerald Litwack Pdf

Receptor Purification is a unique practical two-volume guide to the purification of most receptor types including receptors for neurotransmitters polypeptide hormones steroid hormones and a variety of other ligands involved in many biological processes. Today's methods of receptor purification have evolved from years of research on the mechanisms of receptor actions where they were needed to discover receptor properties and ultimately for use in cloning. Receptor Purification provides a comprehensive source of techniques to enable the purification of cloned receptors as well as a handy reference on purification of receptors from cells and tissues in a physiological context. Designed to give investigators the best possible selection of approaches to a purification problem alternative methods are provided where applicable. In addition Receptor Purification features chapters on specific agents-such as covalent binding ligands-that will greatly simplify some purification processes. Many of the leading laboratories whose work has led to the purification of a wide variety of receptors are represented in the contributions to these two volumes. In some chapters information has been added on the applications of purified receptors while others focus on the cloning of the receptors. The purification of receptors is group-based on functional ligands. The first volume is devoted to the purification of receptors that bind neurotransmitters and a wide variety of polypeptide hormones. Volume 2 covers the steroid receptors and associated proteins such as vitamin D-3 and retinoic acid receptors. Other polypeptide receptors such as those involved in

Author : P. Cuatrecasas
Publisher : Springer Science & Business Media
Page : 246 pages
File Size : 48,8 Mb
Release : 2013-03-09
Category : Science
ISBN : 9789400958661

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Membrane Receptors by P. Cuatrecasas Pdf

Hardly a decade ago, membrane receptors were an attractive but largely unproven concept. Since that time enormous progress has been made, and we are now able to consider receptors much more concretely. Their existence has been established, their binding properties have been determined, and in some cases, they have been highly purified and their physical-chemical properties studied. It is now even possible to visualize microscopically some receptors. This progress has resulted largely from the development of highly powerful methods. These methods are the subject of this volume. Although considerably diverse, different receptors share certain common pro perties, and common problems are encountered in their study. Consequently, a small number of techniques are particularly useful in studying different types of receptors. Thus, it makes sense to speak about membrane receptor methodology. A very apparent problem in the study of membrane receptors is their presence in exceedingly small quantities and in a highly impure state. Therefore, very sensitive and specific techniques are required for their detection, characterization and purification. Such sensitivity and specificity is provided by the ability of receptors to bind certain ligands with very high affmity, and it is not surprising that most of the methods described in this volume depend upon this high affinity binding. The antigen-antibody interaction is of comparable sensitivity and speci ficity. Recently, a number of anti-receptor antibodies have been produced or found to occur spontaneously in auto-immune diseases. Undoubtedly, more will be produced in the future.

Author : Anonim
Publisher : Unknown
Page : 27 pages
File Size : 50,6 Mb
Release : 2024-07-02
Category : Electronic
ISBN : OCLC:490033561

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Receptor Purification by Anonim Pdf

Author : Gerald Litwack
Publisher : Unknown
Page : 501 pages
File Size : 45,8 Mb
Release : 1990
Category : Cell receptors
ISBN : OCLC:37910836

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Receptor Purification by Gerald Litwack Pdf

Author : Gerald Litwack
Publisher : Humana
Page : 0 pages
File Size : 42,8 Mb
Release : 2012-10-16
Category : Medical
ISBN : 146126779X

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Receptor Purification by Gerald Litwack Pdf

The purpose of these volumes is to provide a reference work for the methods of purifying many of the receptors we know about. This becomes increasingly important as full-length recep­ tors are overexpressed in bacteria or in insect cell systems. A major problem for abundantly expressed proteins will be their purification. In addition to purification protocols, many other de­ tails can be found concerning an individual receptor that may not be available in standard texts or monographs. No book of this type is available as a compendium of purification procedures. Receptor Purification provides protocols for the purification of a wide variety of receptors. These include receptors that bind: neurotransmitters, polypeptide hormones, steroid hormones, and ligands for related members of the steroid supergene family and others including receptors involved in bacterial motion. The text of this information is substantial so as to require its publica­ tion in two volumes. Consequently, a division was made by grouping receptors depending upon the nature of their ligands. Thus, in volume 1 there are contributions on serotonin receptors, adrenergic receptors, the purification of GTP-binding proteins, opioid receptors, neurotensin receptor, luteinizing hormone re­ ceptor, human chorionic gonadotropin receptor, follicle stimulat­ ing hormone receptor, thyrotropin receptor, prolactin receptor, epidermal growth factor receptor, platelet derived growth factor receptor, colony stimulating factor receptor, insulinlike growth factor receptors, insulin receptor, fibronectin receptor, interferon receptor, and the cholecystokinin receptor.

Author : Benjamin A. Lieberman
Publisher : Springer Science & Business Media
Page : 377 pages
File Size : 49,8 Mb
Release : 2001-08-10
Category : Medical
ISBN : 9780896037540

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Steroid Receptor Methods by Benjamin A. Lieberman Pdf

A distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA binding characteristics, hormone binding assays, and the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness.

Author : Barry S. Selinsky
Publisher : Springer Science & Business Media
Page : 330 pages
File Size : 46,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592594009

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Membrane Protein Protocols by Barry S. Selinsky Pdf

Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.

Author : P. Michael Conn
Publisher : Academic Press
Page : 455 pages
File Size : 46,7 Mb
Release : 2014-05-10
Category : Science
ISBN : 9781483273686

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The Receptors by P. Michael Conn Pdf

The Receptors, Volume II deals with receptors for somatostatin, vitamin D, insulin, and animal viruses, as well as for the ?2-adrenergic and Ah systems. The significance of translational modifications of receptor ligands is discussed, along with the mechanisms of receptor-ligand interactions. The role of receptors in development and their regulation by tumors are also considered. Comprised of 12 chapters, this volume begins with a detailed account of the vitamin D receptor, paying particular attention to its biochemical and physical properties as well as its mechanism of action. The discussion then turns to experimental discrimination between alternative mechanistic models for the receptor-mediated stimulation of adenylate cyclase; the role of microaggregation in hormone-receptor-effector interactions; and the biology and biochemistry of the Ah receptor. Subsequent chapters explore the interactions of animal viruses with cell surface receptors; insulin receptors; determination of the size of neurotransmitter receptors by radiation inactivation-target size analysis; and protein glycosylation and receptor-ligand interactions. This book will be a valuable resource for students and practitioners in fields ranging from cell biology and biochemistry to physiology, endocrinology, and pharmacology.

Author : Virinder K. Moudgil
Publisher : CRC Press
Page : 442 pages
File Size : 53,9 Mb
Release : 2018-05-04
Category : Science
ISBN : 9781351093149

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Receptor Phosphorylation by Virinder K. Moudgil Pdf

The following chapters comprise invited contributions from eminent scientists who are internationally recognized authorities on the subject. The chapters have been written to provide the reader with adequate background, necessary experimental details, and discussion that is easy to comprehend. The book has been organized into four different sections. The introductory chapter is meant to briefly summarize the contributions of other authors and discuss phosphorylation of receptor systems not covered in the book in detail. The chapters in the second section, on protein kinases and phosphatases, are quite fundamental to the process of phosphorylation and will aid the reader in appreciating the observations and discussions reported in other chapters. The third and fourth sections present discussions on phosphorylation of various receptor systems which are involved in mediating actions of peptide, amine, and steroid hormones. It is hoped that this book will serve as a valuable resource and will be useful to all workers and students in the area of receptor-ligand interactions.

Author : Anonim
Publisher : Elsevier
Page : 519 pages
File Size : 40,8 Mb
Release : 1995-03-22
Category : Medical
ISBN : 0080536441

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Receptor Molecular Biology by Anonim Pdf

The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Cloning Expression systems Signal transduction Structure-function techniques Antireceptor antibodies Regulation 3-D receptor modeling and computational probing

Author : James Steven Wild
Publisher : Unknown
Page : 328 pages
File Size : 53,7 Mb
Release : 1995
Category : Electronic
ISBN : UCAL:X55761

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Characterization and Partial Purification of a Lung Ryanodine Receptor by James Steven Wild Pdf

Author : Gabriel Berstein
Publisher : CRC Press
Page : 380 pages
File Size : 55,5 Mb
Release : 2019-04-24
Category : Medical
ISBN : 9780429525261

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G Protein-Coupled Receptors by Gabriel Berstein Pdf

Covering recently developed methods in membrane-bound receptors, this book emphasizes receptor structure and function, knowledge of which is essential to the study of signal transduction. G Protein-Coupled Receptors has culled contributors from domestic and international sources, providing a broad base of knowledge. Some topics covered are the r

Author : Petrus C. van der Linde
Publisher : Unknown
Page : 150 pages
File Size : 54,5 Mb
Release : 1986
Category : Electronic
ISBN : CORNELL:31924063899409

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Modulation, Purification and Function of a Soluble Auxin Receptor by Petrus C. van der Linde Pdf