Recombinant Proteins From Plants

Author : Charles Cunningham,Andrew J. R. Porter
Publisher : Springer Science & Business Media
Page : 309 pages
File Size : 41,8 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781603272605

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Recombinant Proteins from Plants by Charles Cunningham,Andrew J. R. Porter Pdf

Recombinant Proteins from Plants is one of the most exciting and fastest developing areas in biology. The latest molecular techniques are being applied to the exploitation of plants as novel expression systems for the p- duction and overproduction of heterologous and native proteins. Transgenic plant technology is currently used in three broad areas: the expression of - combinant proteins to improve crop quality by increasing disease/pest res- tance or increasing tolerance to stress, optimizing plant productivity and yield by the genetic manipulation of metabolic pathways, and the large-scale co- effective production of recombinant proteins for use as specialist industrial or therapeutic biomolecules. The intention of Recombinant Proteins from Plants is to provide c- prehensive and detailed protocols covering all the latest molecular approaches. Because the production oftransgenic plants has become routine in many la- ratories, coverage is also given to some of the more "classical" approaches to the separation, analysis, and characterization of recombinant proteins. The book also includes areas of research that we believe will become increasingly important in the near future: efficient transformation of monocots with Agrobacterium optimizing the stability of recombinant proteins, and a section highlighting the immunotherapeutic potential of plant-expressed proteins.

Author : Stefan Schillberg,Holger Spiegel
Publisher : Humana
Page : 346 pages
File Size : 54,6 Mb
Release : 2022-05-27
Category : Science
ISBN : 1071622404

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Recombinant Proteins in Plants by Stefan Schillberg,Holger Spiegel Pdf

This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and purification, different strategies to optimise productivity, cloning and fusion protein approaches, and the regulation and freedom to operate analysis of plant-produced proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Proteins in Plants: Methods and Protocols aims to be useful to newcomers and experienced researchers interested in expanding their expertise in the field of plant-based protein production. Chapters 6, 8 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : John A. Howard,Elizabeth E. Hood
Publisher : Springer
Page : 282 pages
File Size : 51,9 Mb
Release : 2014-09-01
Category : Science
ISBN : 9783662438367

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Commercial Plant-Produced Recombinant Protein Products by John A. Howard,Elizabeth E. Hood Pdf

Attention has recently turned to using plants as hosts for the production of commercially important proteins. The twelve case studies in this volume present successful strategies for using plants to produce industrial and pharmaceutical proteins and vaccine antigens. They examine in detail projects that have commercial potential or products that have already been commercialized, illustrating the advantages that plants offer over bacterial, fungal or animal cell-culture hosts. There are many indications that plant protein production marks the beginning of a new paradigm for the commercial production of proteins that, over the next decade, will expand dramatically.

Author : Jacqueline MacDonald,Igor Kolotilin,Rima Menassa
Publisher : Humana
Page : 0 pages
File Size : 46,6 Mb
Release : 2015-11-27
Category : Science
ISBN : 1493932888

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Recombinant Proteins from Plants by Jacqueline MacDonald,Igor Kolotilin,Rima Menassa Pdf

This volume provides up-to-date scientific achievements from the world’s top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.

Author : Allison R. Kermode
Publisher : John Wiley & Sons
Page : 496 pages
File Size : 46,6 Mb
Release : 2018-03-12
Category : Science
ISBN : 9781118801482

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Molecular Pharming by Allison R. Kermode Pdf

A single volume collection that surveys the exciting field of plant-made pharmaceuticals and industrial proteins This comprehensive book communicates the recent advances and exciting potential for the expanding area of plant biotechnology and is divided into six sections. The first three sections look at the current status of the field, and advances in plant platforms and strategies for improving yields, downstream processing, and controlling post-translational modifications of plant-made recombinant proteins. Section four reviews high-value industrial and pharmacological proteins that are successfully being produced in established and emerging plant platforms. The fifth section looks at regulatory challenges facing the expansion of the field. The final section turns its focus toward small molecule therapeutics, drug screening, plant specialized metabolites, and plants as model organisms to study human disease processes. Molecular Pharming: Applications, Challenges and Emerging Areas offers in-depth coverage of molecular biology of plant expression systems and manipulation of glycosylation processes in plants; plant platforms, subcellular targeting, recovery, and downstream processing; plant-derived protein pharmaceuticals and case studies; regulatory issues; and emerging areas. It is a valuable resource for researchers that are in the field of plant molecular pharming, as well as for those conducting basic research in gene expression, protein quality control, and other subjects relevant to molecular and cellular biology. Broad ranging coverage of a key area of plant biotechnology Describes efforts to produce pharmaceutical and industrial proteins in plants Provides reviews of recent advances and technology breakthroughs Assesses realities of regulatory and cost hurdles Forward looking with coverage of small molecule technologies and the use of plants as models of human disease processes Providing wide-ranging and unique coverage, Molecular Pharming: Applications, Challenges and Emerging Areas will be of great interest to the plant science, plant biotechnology, protein science, and pharmacological communities.

Author : Elizabeth E. Hood,J.A. Howard
Publisher : Springer Science & Business Media
Page : 209 pages
File Size : 42,6 Mb
Release : 2013-03-09
Category : Medical
ISBN : 9789401726931

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Plants as Factories for Protein Production by Elizabeth E. Hood,J.A. Howard Pdf

This exciting volume Plants as Factories for Protein Production, edited by Drs. Elizabeth E. Hood and John A. Howard, contains chapters by experts in the field of molecular farming. The information within addresses the leading plant systems for recombinant protein production, as well as the progress being made in leading product categories - human pharmaceuticals, animal health, and industrial enzymes. More importantly, the book includes chapters that address the hot topics of production, containment, regulatory, and legal aspects that are quickly coming to the forefront of the industry. This most timely text is appropriate for graduate students and post-doctoral fellows, as well as being a key text for faculty, pharmaceutical producers, and industrial enzyme users.

Author : Gerd Gellissen
Publisher : John Wiley & Sons
Page : 429 pages
File Size : 41,9 Mb
Release : 2006-03-06
Category : Science
ISBN : 9783527604418

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Production of Recombinant Proteins by Gerd Gellissen Pdf

While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems -- mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example. With a foreword by Herbert P. Schweizer, Colorado State University, USA: "As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."

Author : Anonim
Publisher : Academic Press
Page : 384 pages
File Size : 54,7 Mb
Release : 2021-11-04
Category : Science
ISBN : 9780323907385

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Recombinant Protein Expression: Eukaryotic hosts by Anonim Pdf

Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more. Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250, and Expression of recombinant antibodies in Leishmania tarentolae. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology serial Updated release includes the latest information on Recombinant Protein Expression

Author : Kathleen L Hefferon
Publisher : CABI
Page : 182 pages
File Size : 51,5 Mb
Release : 2014-10-29
Category : Science
ISBN : 9781780643434

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Plant-derived Pharmaceuticals by Kathleen L Hefferon Pdf

Describing recent developments in the engineering and generation of plants as production platforms for biopharmaceuticals, this book includes both vaccines and monoclonal antibodies. It has a particular emphasis on targeting diseases which predominate in less developed countries, encompassing the current state of technologies and describing expression systems and applications. This book also includes a variety of vaccine case studies, protecting against pervasive infectious diseases such as rabies, influenza and HIV.

Author : Ingo Potrykus,German Spangenberg
Publisher : Springer Science & Business Media
Page : 370 pages
File Size : 43,6 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783642792472

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Gene Transfer to Plants by Ingo Potrykus,German Spangenberg Pdf

Author : Charis M. Galanakis
Publisher : Academic Press
Page : 358 pages
File Size : 54,5 Mb
Release : 2019-05-30
Category : Health & Fitness
ISBN : 9780128172865

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Proteins: Sustainable Source, Processing and Applications by Charis M. Galanakis Pdf

Proteins: Sustainable Source, Processing and Applications addresses sustainable proteins, with an emphasis on proteins of animal origin, plant-based and insect proteins, microalgal single cell proteins, extraction, production, the stability and bioengineering of proteins, food applications (e.g. encapsulation, films and coatings), consumer behavior and sustainable consumption. Written in a scientific manner to meet the needs of chemists, food scientists, technologists, new product developers and academics, this book addresses the health effects and properties of proteins, highlights sustainable sources, processes and consumption models, and analyzes the potentiality of already commercialized processes and products. This book is an integral resource that supports the current applications of proteins in the food industry, along with those that are currently under development. Supports the current applications of proteins in the food industry, along with those that are under development Connects the properties and health effects of proteins with sustainable sources, recovery procedures, stability and encapsulation Explores industrial applications that are affected by aforementioned aspects

Author : Aiming Wang,Shengwu Ma
Publisher : Springer Science & Business Media
Page : 284 pages
File Size : 51,8 Mb
Release : 2011-10-14
Category : Science
ISBN : 9789400722170

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Molecular Farming in Plants: Recent Advances and Future Prospects by Aiming Wang,Shengwu Ma Pdf

Molecular farming in plants is a relatively young subject of sciences. As plants can offer an inexpensive and convenient platform for the large-scale production of recombinant proteins with various functions, the driven force from the giant market for recombinant protein pharmaceuticals and industrial enzymes makes this subject grow and advance very quickly. To summarize recent advances, current challenges and future directions in molecular farming, international authorities were invited to write this book for researchers, teachers and students who are interested in this subject. This book, with the focus on the most advanced cutting-edge breakthroughs, covers all the essential aspects of the field of molecular farming in plants: from expression technologies to downstream processing, from products to safety issues, and from current advances and holdups to future developments.

Author : Paulina Balbas,Argelia Lorence
Publisher : Springer Science & Business Media
Page : 505 pages
File Size : 53,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592597741

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Recombinant Gene Expression by Paulina Balbas,Argelia Lorence Pdf

Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.

Author : Anonim
Publisher : UCANR Publications
Page : 12 pages
File Size : 45,6 Mb
Release : 2024-06-29
Category : Electronic
ISBN : 9781601072542

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Production of Therapeutic Proteins in Plants by Anonim Pdf

Author : Aiming Wang,Shengwu Ma
Publisher : Springer
Page : 280 pages
File Size : 50,8 Mb
Release : 2011-10-21
Category : Science
ISBN : 9400722184

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Molecular Farming in Plants: Recent Advances and Future Prospects by Aiming Wang,Shengwu Ma Pdf

Molecular farming in plants is a relatively young subject of sciences. As plants can offer an inexpensive and convenient platform for the large-scale production of recombinant proteins with various functions, the driven force from the giant market for recombinant protein pharmaceuticals and industrial enzymes makes this subject grow and advance very quickly. To summarize recent advances, current challenges and future directions in molecular farming, international authorities were invited to write this book for researchers, teachers and students who are interested in this subject. This book, with the focus on the most advanced cutting-edge breakthroughs, covers all the essential aspects of the field of molecular farming in plants: from expression technologies to downstream processing, from products to safety issues, and from current advances and holdups to future developments.