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Author : Zbigniew Stanisław Warkocki
Publisher : Unknown
Page : 161 pages
File Size : 41,7 Mb
Release : 2012
Category : Electronic
ISBN : 3868444394
Author : Stefan Stamm,Chris Smith,Reinhard Lührmann
Publisher : John Wiley & Sons
Page : 660 pages
File Size : 50,8 Mb
Release : 2012-08-01
Category : Science
ISBN : 9783527647989
This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing in twelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative pre-mRNA splicing in vitro and in vivo, Manipulation of splicing events, and Bioinformatic analysis of alternative splicing. A theoretical introduction and practical guide for molecular biologists, geneticists,clinicians and every researcher interested in alternative splicing. Website: www.wiley-vch.de/home/splicing
Author : Ane Sesma,Tobias von der Haar
Publisher : Springer Science & Business Media
Page : 396 pages
File Size : 52,8 Mb
Release : 2014-04-15
Category : Science
ISBN : 9783319056876
This book presents an overview of the RNA networks controlling gene expression in fungi highlighting the remaining questions and future challenges in this area. It covers several aspects of the RNA-mediated mechanisms that regulate gene expression in model yeasts and filamentous fungi, organisms of great importance for industry, medicine and agriculture. It is estimated that there are more than one million fungal species on the Earth. Despite their diversity (saprophytic, parasitic and mutualistic), fungi share common features distinctive from plants and animals and have been grouped taxonomically as an independent eukaryotic kingdom. In this book, 15 chapters written by experts in their fields cover the RNA-dependent processes that take place in a fungal cell ranging from formation of coding and non-coding RNAs to mRNA translation, ribosomal RNA biogenesis, gene silencing, RNA editing and epigenetic regulation.
Author : Max L. Birnstiel
Publisher : Springer Science & Business Media
Page : 221 pages
File Size : 43,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642730207
In the past decade a most interesting story of the role played by the small nuclear RNPs, catalysts in RNA processing, has unfolded. Early investigations of the structure of these particles gave rise to hypotheses of their functions. As described in this book, these have been confirmed or are being tested by biochemical ex perimentation and most recently by the powerful technique of genetic analysis. Both biochemical and genetical approaches will also determine to what degree, and how, snRNPs and their cofactors participate in the differential expression of genes. As the details emerge and the number of snRNP species increases way beyond the six initially identified, one feels at the threshold of even greater things to come. The book covers many results in one of the most rapidly expanding fields of molecular biology. For this reason alone the specialist may detect some omissions and shortcomings. I hope they will be few. Instead of presenting a collation of conference reports with much overlap between them, this book has been written specially for the purpose of surveying the literature up to early 1987 in the snRNP field in a coherent manner, each of the seven chapters having been produced by connoisseurs of their field. While not each and every snRNP story can be covered in such a book, I hope that it will provide enjoyable and stimulating reading.
Author : Anonim
Publisher : Unknown
Page : 1364 pages
File Size : 45,9 Mb
Release : 1994
Category : Medicine
ISBN : MINN:31951D01105027V
Author : Katherine Anne Senn
Publisher : Unknown
Page : 0 pages
File Size : 47,7 Mb
Release : 2023
Category : Electronic
ISBN : OCLC:1433052593
Pre-mRNA splicing, the removal of noncoding introns to form a protein coding mRNA, is a crucial step in eukaryotic gene expression. The spliceosome, a macromolecular complex made up of five small nuclear RNAs (snRNAs) and dozens of proteins organized into small nuclear ribonucleoproteins (snRNPs), catalyzes splicing in two steps. These steps are highly regulated to ensure correct mRNA isoform production and protein expression. Core components of the spliceosome as well as additional auxiliary factors tune splicing for accuracy and regulation of alternative splicing outcomes. Because of the complexity of splicing regulation, there are many open questions about the factors involved and the regulation of alternative splicing. This thesis addresses the functions of two yeast proteins involved in splicing regulation and an alternatively spliced gene in humans.In chapters 2 and 3, I discuss the novel yeast splicing factor Fyv6. Genetic and biochemical evidence supports a role for Fyv6 in the second step of splicing, exon ligation. A collaborator has determined the structure of a Fyv6-containing spliceosome by cryo-electron microscopy. Using this structure, we have used biochemical and transcriptomics experiments to elucidate how Fyv6 affects 3' splice site choice. Yeast splicing regulatory factor Mer1 interacts with introns and the U1 snRNP to enhance splicing of a subset of meiotically-regulated pre-mRNAs by an as-yet undetermined mechanism. With the goal of elucidating the mechanism, I recapitulated Mer1-dependent splicing in vitro and used colocalization single molecule microscopy to examine how Mer1 affects the dynamics of U1 binding to pre-mRNAs (Chapter 4). Our results indicate Mer1 promotes splicing without significantly altering the observed distributions of U1 binding events. This study lays the groundwork for future studies of splicing regulation by Mer1 and other factors. Finally, I studied the expression and alternative splicing of human glucose transporter family member GLUT8 (Chapter 5). Multiple alternatively spliced GLUT8 mRNA isoforms exist, but only some encode viable proteins. One that does not is upregulated in cancers, which reduces GLUT8 protein expression. Collaborators showed that based on its localization, inability to transport glucose, and release of a cleaved peptide, the main functional isoform of GLUT8 may be a metabolic sensor.
Author : Adrian Krainer,James Manley,Timothy W. Nilsen
Publisher : Unknown
Page : 280 pages
File Size : 55,6 Mb
Release : 1997
Category : Genetic transcription
ISBN : UCLA:L0074750803
Author : Anonim
Publisher : Unknown
Page : 372 pages
File Size : 49,8 Mb
Release : 1991
Category : Genetic transcription
ISBN : UCAL:B4564418
Author : Anonim
Publisher : Unknown
Page : 340 pages
File Size : 48,7 Mb
Release : 1992
Category : Biology
ISBN : UCLA:L0067532259
Author : California Institute of Technology. Division of Biology
Publisher : Unknown
Page : 696 pages
File Size : 45,6 Mb
Release : 1993
Category : Biology
ISBN : STANFORD:36105016177946
Author : Anonim
Publisher : Unknown
Page : 860 pages
File Size : 47,6 Mb
Release : 1993
Category : Nucleic acids
ISBN : UVA:X002542902
Author : Anonim
Publisher : Unknown
Page : 880 pages
File Size : 42,5 Mb
Release : 1995
Category : Agriculture
ISBN : CORNELL:31924063043297
Author : Anonim
Publisher : Unknown
Page : 634 pages
File Size : 48,5 Mb
Release : 1999
Category : Agriculture
ISBN : UOM:39015055285046
Author : Anonim
Publisher : Unknown
Page : 448 pages
File Size : 40,5 Mb
Release : 1997
Category : Nucleic acids
ISBN : UVA:X006074590
Author : Anonim
Publisher : Unknown
Page : 1902 pages
File Size : 45,5 Mb
Release : 2003
Category : Medicine
ISBN : MINN:31951P00880601L
Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.