Rna Based Technologies For Functional Genomics In Plants
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RNA-Based Technologies for Functional Genomics in Plants by Guiliang Tang,Sachin Teotia,Xiaoqing Tang,Deepali Singh Pdf
This book offers a unique and comprehensive overview of key RNA-based technologies, as well as their development and applications for the functional genomics of plant coding and non-coding genes. It focuses on the latest as well as classical RNA-based techniques used for studies on small RNAs, long non-coding RNAs and protein-coding genes. These techniques chiefly focus on target mimics (TMs) and short tandem target mimics (STTMs) for small RNAs, and artificial microRNAs (amiRNAs), RNA interference (RNAi) and CRISPR/Cas for genes. Furthermore, the book discusses the latest trends in the field and various modifications of the above-mentioned approaches, and explores how these RNA-based technologies have been developed, applied and validated as essential technologies in plant functional genomics. RNA-based technologies, their mechanisms of action, their advantages and disadvantages, and insights into the further development and applications of these technologies in plants are discussed. These techniques will enable the users to functionally characterize genes and small RNAs through silencing, overexpression and editing. Gathering contributions by globally respected experts, the book will appeal to students, teachers and scientists in academia and industry who are interested in horticulture, genetics, pathology, entomology, physiology, molecular genetics and breeding, in vitro culture & genetic engineering, and functional genomics.
Functional Genomics by Germana Meroni,Francesca Petrera Pdf
This book titled "Functional Genomics" contains a selection of chapters focused on crucial topics in functional genomics, from the analysis of the genetic code, to the understanding of the role of the different genes and to the proteomic implications. The book provides an overview on basic issues and some of the recent developments in medicinal science and technology. Covering all the aspects involved in such a broad theme as functional genomics and in all its applications would be impossible within the same book. The different chapters represent a brief introduction to the topic, connecting the most promising developments in functional genomics technologies, focusing on specific applications in biomedicine, agro-food technologies and zootechniques.
Applied Plant Genomics and Biotechnology by Palmiro Poltronieri,Yiguo Hong Pdf
Applied plant genomics and biotechnology reviews the recent advancements in the post-genomic era, discussing how different varieties respond to abiotic and biotic stresses, investigating epigenetic modifications and epigenetic memory through analysis of DNA methylation states, applicative uses of RNA silencing and RNA interference in plant physiology and in experimental transgenics, and plants modified to produce high-value pharmaceutical proteins. The book provides an overview of research advances in application of RNA silencing and RNA interference, through Virus-based transient gene expression systems, Virus induced gene complementation (VIGC), Virus induced gene silencing (Sir VIGS, Mr VIGS) Virus-based microRNA silencing (VbMS) and Virus-based RNA mobility assays (VRMA); RNA based vaccines and expression of virus proteins or RNA, and virus-like particles in plants, the potential of virus vaccines and therapeutics, and exploring plants as factories for useful products and pharmaceuticals are topics wholly deepened. The book reviews and discuss Plant Functional Genomic studies discussing the technologies supporting the genetic improvement of plants and the production of plant varieties more resistant to biotic and abiotic stresses. Several important crops are analysed providing a glimpse on the most up-to-date methods and topics of investigation. The book presents a review on current state of GMO, the cisgenesis-derived plants and novel plant products devoid of transgene elements, discuss their regulation and the production of desired traits such as resistance to viruses and disease also in fruit trees and wood trees with long vegetative periods. Several chapters cover aspects of plant physiology related to plant improvement: cytokinin metabolism and hormone signaling pathways are discussed in barley; PARP-domain proteins involved in Stress-Induced Morphogenetic Response, regulation of NAD signaling and ROS dependent synthesis of anthocyanins. Apple allergen isoforms and the various content in different varieties are discussed and approaches to reduce their presence. Euphorbiaceae, castor bean, cassava and Jathropa are discussed at genomic structure, their diseases and viruses, and methods of transformation. Rice genomics and agricultural traits are discussed, and biotechnology for engineering and improve rice varieties. Mango topics are presented with an overview of molecular methods for variety differentiation, and aspects of fruit improvement by traditional and biotechnology methods. Oilseed rape is presented, discussing the genetic diversity, quality traits, genetic maps, genomic selection and comparative genomics for improvement of varieties. Tomato studies are presented, with an overview on the knowledge of the regulatory networks involved in flowering, methods applied to study the tomato genome-wide DNA methylation, its regulation by small RNAs, microRNA-dependent control of transcription factors expression, the development and ripening processes in tomato, genomic studies and fruit modelling to establish fleshy fruit traits of interest; the gene reprogramming during fruit ripening, and the ethylene dependent and independent DNA methylation changes. provides an overview on the ongoing projects and activities in the field of applied biotechnology includes examples of different crops and applications to be exploited reviews and discusses Plant Functional Genomic studies and the future developments in the field explores the new technologies supporting the genetic improvement of plants
The Handbook of Plant Functional Genomics by Guenter Kahl,Khalid Meksem Pdf
In this incisive, concise overview of this booming field, the editors -- two of the leading figures in the field with a proven track record -- combine their expertise to provide an invaluable reference on the topic. Following a treatment of transcriptome analysis, the book goes on to discuss replacement and mutation analysis, gene silencing and computational analysis. The whole is rounded off with a look at emerging technologies. Each chapter is accompanied by a concise overview, helping readers to quickly identify topics of interest, while important, carefully selected words and concepts are explained in a handy glossary. Equally accessible to both experienced scientists and newcomers to the field.
RNA Interference Technology by Krishnarao Appasani Pdf
RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.
CRISPR and Plant Functional Genomics by Jen-Tsung Chen Pdf
CRISPR is a crucial technology in plant physiology and molecular biology resulting in more sustainable agricultural practices, including outcomes of better plant stress tolerance and crop improvement. CRISPR and Plant Functional Genomics explores ways to release the potential of plant functional genomics, one of the prevailing topics in plant biology and a critical technology for speed and precision crop breeding. This book presents achievements in plant functional genomics and features information on diverse applications using the emerging CRISPR-based genome editing technologies producing high-yield, disease-resistant, and climate-smart crops. It also includes theories on organizing strategies for upgrading the CRISPR system to increase efficiency, avoid off-target effects, and produce transgene-free edited crops. Features: Presents CRISPR-based technologies, releasing the potential of plant functional genomics Provides methods and applications of CRISPR/Cas-based plant genome editing technologies Summarizes achievements of speed and precision crop breeding using CRISPR-based technologies Illustrates strategies to upgrade the CRISPR system Supports the UN’s sustainable development goals to develop future climate-resilient crops CRISPR and Plant Functional Genomics provides extensive knowledge of CRISPR-based technologies and plant functional genomics, and is an ideal reference for researchers, graduate students, and practitioners in the field of plant sciences as well as agronomy and agriculture.
Discover cutting-edge knowledge for engineering a more productive and environment-friendly agriculture! In Plant Functional Genomics, you’ll find a cross-section of state-of-the-art research on the biological function of plant genes and how they work together in health and disease. World-leading scientists in the field present breakthrough techniques, discuss the results of projects aimed at dissecting particular plant functions, and provide an overview on the state of functional genomics for several plant and plant-related species. With figures, tables, and illustrations, this book will help scientists, researchers, and advanced students in botany find new ways of creating novel plant forms to better serve the needs of a rapidly expanding human population. Plant Functional Genomics will increase your understanding of gene networks and systems rules, as well as gene expression during specific conditions or development or treatments. This important resource contains a wealth of data generated by various plant genome sequencing projects, including the newest results from experiments with Arabidopsis thaliana—the first plant to be completely sequenced. This book also contains innovative research on: T-DNA mutagenesis transcriptomics and metabolic profiling in plants large-scale yeast two-hybrid analyses the exceptional model system of Chlamydomonas genomics functional genomics in rice, maize, and Physcomitrella prospects for functional genomics in a new model grass chloroplast and plant mitochondrial proteomics plant transporters so much more Plant Functional Genomics will help speed up the identification and isolation of genes that might be of interest with respect to diverse biological questions. This valuable contribution to the field clarifies the challenges yet to be faced and the opportunities that could some day expand the frontiers of plant sciences.
Functional genomics is a young discipline whose origin can be traced back to the late 1980s and early 1990s, when molecular tools became available to determine the cellular functions of genes. Today, functional genomics is p- ceived as the analysis, often large-scale, that bridges the structure and organi- tion of genomes and the assessment of gene function. The completion in 2000 of the genome sequence of Arabidopsis thaliana has created a number of new and exciting challenges in plant functional genomics. The immediate task for the plant biology community is to establish the functions of the approximately 25,000 genes present in this model plant. One major issue that will remain even after this formidable task is c- pleted is establishing to what degree our understanding of the genome of one model organism, such as the dicot Arabidopsis, provides insight into the or- nization and function of genes in other plants. The genome sequence of rice, completed in 2002 as a result of the synergistic interaction of the private and public sectors, promises to significantly enrich our knowledge of the general organization of plant genomes. However, the tools available to investigate gene function in rice are lagging behind those offered by other model plant systems. Approaches available to investigate gene function become even more limited for plants other than the model systems of Arabidopsis, rice, and maize.
The openings offered by functional genomics reconciles organism biology and molecular biology, in order to define an integrative biology that should allow new insights about how a phenotype is built up from a genotype in interaction with its environment. This book covers a wide area of concepts and methods in genomics. This range from international
OMICs-based Techniques for Global Food Security by Sajid Fiaz,Channapatna S. Prakash Pdf
Forward-thinking resource discussing how to integrate OMICs and novel genome editing technologies for sustainable crop production OMICS-based Techniques for Global Food Security provides an in-depth understanding of the mechanisms of OMICs techniques for crop improvement, details how OMICs techniques can contribute to identifying genes and traits with economic benefits, and explains how to develop crop plants with improved yield, quality, and resistance to stresses through genome editing technologies, providing evidence on the developments of climate resilient crops via applications of genome editing techniques throughout. The text covers the application of OMICs in crop plants, the integration of bioinformatics and multi-OMICs for precision breeding, de-novo domestication, CRISPR/Cas system for crop improvement, hybrid seed production, transgene free breeding, regulation for genome edit crops, bioinformatics and genome editing, and other topics related OMICs and genome editing. The text also includes a chapter on the global regulations for genome edited crops, and explains how these regulations influence novel plant breeding techniques in their adopted countries. Written by two highly qualified academics, OMICs-based Techniques for Global Food Security covers sample topics such as: Crops genome sequencing and their application for crop improvement, and functional characterization of cereals genome The role of OMICs based technologies in plant sciences and utilization of different multi-OMICs approaches for crop improvement Genomic database and genetic resource of cereals, speed breeding for rapid crop improvement, and evolution of genome editing technologies CRISPR system discovery, history, and future perspective, and CRISPR/Cas system for biotic and abiotic stress resistance in cereals Providing a collection of recent literature focusing on developments and applications of OMICs based technologies for crop improvement, OMICs-based Techniques for Global Food Security is an important read for plant breeders, molecular biologists, researchers, postdoctoral fellows, and students in disciplines for developing crops with high yield and nutritional potential.
Gene Silencing by RNA Interference by Muhammad Sohail Pdf
Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by r
OMICS-Based Approaches in Plant Biotechnology by Rintu Banerjee,Garlapati Vijay Kumar,S.P. Jeevan Kumar Pdf
Burgeoning world population, decreased water supply and land resources, coupled with climate change, result in severe stress conditions and a great threat to the global food supply. To meet these challenges, exploring Omics Technologies could lead to improved yields of cereals, tubers and grasses that may ensure food security. Improvement of yields through crop improvement and biotechnological means are the need-of-the-hour, and the current book “OMICS-Based Approaches in Plant Biotechnology”, reviews the advanced concepts on breeding strategies, OMICS technologies (genomics, transcriptomics and metabolomics) and bioinformatics that help to glean the potential candidate genes/molecules to address unsolved problems related to plant and agricultural crops. The first six chapters of the book are focused on genomics and cover sequencing, functional genomics with examples on insecticide resistant genes, mutation breeding and miRNA technologies. Recent advances in metabolomics studies are elucidated in the next 3 chapters followed by 5 chapters on bioinformatics and advanced techniques in plant biotechnology and crop breeding. The information contained in the volume will help plant breeders, plant biotechnologists, plant biochemists, agriculture scientists and researchers in using this applied research to focus on better crop breeding and stress adaptation strategies.
Advances and Challenges of RNAi Based Technologies for Plants by Susana Araújo,Pedro Fevereiro,Matthias Fladung,Bruno Mezzetti,Guy Smagghe,Jeremy Bruton Sweet Pdf
From Functional Genomics to Biotechnology in Ornamental Plants by Swee-Suak Ko,Akira Kanno,Raquel Sánchez-Pérez,Hsin-Hung Yeh,Annette Hohe,Mariana Mondragón-Palomino Pdf
For centuries plants of a broad taxonomical background have been bred and commercialized because of the beauty of their flowers. However, until recently genomic analyses of ornamentals remained a challenge because of their large genome sizes and high ploidy levels. In the last decade, increasingly affordable sequencing technologies and powerful bioinformatic approaches resulted in the complete sequencing of several horticultural species genomes and the characterization of their transcriptomes. These developments enabled research on many challenging topics. This Research Topic gives you a primer into them by featuring a broad range of original research contributions on some of the most active areas of ornamental plant research: the genetic basis of flower morphology, scent, and color, the genetic regulation of physiology as well as the epigenetic factors affecting vegetative development. In this context, one of the most significant hurdles to functional genetic studies in ornamentals is achieving efficient genetic transformation. Several articles in this Research Topic describe strategies to tackle this challenge and present insights into the way transgene activity renders novel flower phenotypes.
Plant Disease Management in the Post-Genomic Era: from Functional Genomics to Genome Editing by Sabrina Sarrocco,Alfredo Herrera-Estrella,David B. Collinge Pdf
Crop plants are continuously under attack by pathogens, both during pre and post-harvest stages, often causing economically important food losses. Chemical treatments can pose a serious treat to human health and the environment. Furthermore, there is an increasing market, especially in OECD countries for organic produce, for which most pesticides and inorganic fertilisers are unacceptable. There is therefore a pressing need to develop more effective, sustainable and environmentally friendly tools for disease control. The use of beneficial microorganisms for the control of plant pathogens is very attractive, and the availability of novel molecular techniques and a plethora of genomic information open unexplored avenues for plant protection approaches. Genome-enabled integration of research became a major strategy in the era of the 2000's (the post-genomic era) and set the basis for a different way to understand interactions in plant-pathogen-beneficial microorganisms systems. In recent decades we have moved from the sequencing of single genomes, to the comparison of different genomes, their expression (from single organism to communities) and, more recently, we can apply novel techniques to edit a genome in a precise manner. Together with the complete sequencing of plant genomes, the genome sequences of plant pathogens as well as that of some beneficial organisms (bacteria, fungi, viruses), and the furnished information on their virulence, gave support to relatively new strategies such as transcriptome, proteome, metabolome and secretome analysis. Analyses of avirulence and resistance genes, their products and the cross-talking mechanisms, as well as proteins and metabolites - at an "omics" level - in highly performing beneficial microorganisms/pathogens interactions, represents a major contribution to plant protection, providing information at an unprecedented level of resolution. While single genomes are explored to infer the range of biological activities accomplished by a single organism or species, and comparative genomics allows evaluating the diversity and evolution of biochemical pathways adopted by individual species to perform a common function, information is being obtained about processes carried out by a diverse set of organisms interacting with each other. Environmental genomics, popularized by the metagenome concept, can generate billions of DNA sequences from a given environment furnishing a comparative assessment of a community in situ. Metagenomics (sequence-based approaches applied across genomes in an environment) provides information about functional capabilities and responses of organism (plant, pathogen and beneficial agent) assemblages in different niches, giving a different perspective in the management of plant diseases at a multitrophic level. Advances in functional genomics and genome editing approaches have recently provided new tools to manage the plant-pathogen-beneficial microorganism system, for preventing or controlling disease. The use of RNA-based technologies is extremely appealing, and these include artificial micro-RNA and transacting small interfering RNA, which are currently being used for generating plant virus-resistant plants, thus fostering plant virus control researches. Finally, targeted genome editing strategies - exemplified by, but not limited to, the CRISPR-Cas technique - are among the most modern ways for inducing targeted deletions, insertions and precise changes in the genome of host plants or pathogens, as well as in the genomes of beneficial microorganisms. The aim of the present Research Topic is to give an exhaustive and up to date overview of examples of genomic techniques (genome sequencing, genome comparison, transcriptomics, metagenomics, RNA based technologies and genome editing strategies) applied to plants, pathogens or beneficial microorganisms to promote the exploitation of these modern tools as a new frontier in plant disease management.
