Rna Detection

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RNA Detection

Imre Gaspar
RNA Detection Book PDF

Author : Imre Gaspar
Publisher : Humana Press
Page : 492 pages
File Size : 48,5 Mb
Release : 2017-11-15
Category : Medical
ISBN : 149397212X

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RNA Detection by Imre Gaspar Pdf

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

RNA Detection

Imre Gaspar
RNA Detection Book PDF

Author : Imre Gaspar
Publisher : Unknown
Page : 492 pages
File Size : 47,7 Mb
Release : 2018
Category : Biomedicine
ISBN : 1493972138

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RNA Detection by Imre Gaspar Pdf

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

RNA Detection and Visualization

Jeffrey E. Gerst
RNA Detection and Visualization Book PDF

Author : Jeffrey E. Gerst
Publisher : Humana Press
Page : 498 pages
File Size : 51,9 Mb
Release : 2011-08-24
Category : Science
ISBN : 1617790060

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RNA Detection and Visualization by Jeffrey E. Gerst Pdf

With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.

RNA and DNA Diagnostics

Volker A. Erdmann,Stefan Jurga,Jan Barciszewski
RNA and DNA Diagnostics Book PDF

Author : Volker A. Erdmann,Stefan Jurga,Jan Barciszewski
Publisher : Springer
Page : 349 pages
File Size : 47,8 Mb
Release : 2015-06-10
Category : Medical
ISBN : 9783319173054

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RNA and DNA Diagnostics by Volker A. Erdmann,Stefan Jurga,Jan Barciszewski Pdf

The aim of molecular diagnostics is preferentially to detect a developing disease before any symptoms appear. There has been a significant increase, fueled by technologies from the human genome project, in the availability of nucleic acid sequence information for all living organisms including bacteria and viruses. When combined with a different type of instrumentation applied, the resulting diagnostics is specific and sensitive. Nucleic acid-based medical diagnosis detects specific DNAs or RNAs from the infecting organism or virus and a specific gene or the expression of a gene associated with a disease. Nucleic acid approaches also stimulate a basic science by opening lines of inquiry that will lead to greater understanding of the molecules at the center of life. One can follow Richard Feynman’s famous statement “What I cannot create, I do not understand.”

Nucleic Acid Detection

Dmitry M. Kolpashchikov,Yulia V. Gerasimova
Nucleic Acid Detection Book PDF

Author : Dmitry M. Kolpashchikov,Yulia V. Gerasimova
Publisher : Humana
Page : 0 pages
File Size : 42,7 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493960415

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Nucleic Acid Detection by Dmitry M. Kolpashchikov,Yulia V. Gerasimova Pdf

In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.

RNA Abundance Analysis

Hailing Jin,Walter Gassmann
RNA Abundance Analysis Book PDF

Author : Hailing Jin,Walter Gassmann
Publisher : Humana Press
Page : 248 pages
File Size : 51,5 Mb
Release : 2016-05-01
Category : Medical
ISBN : 1493959166

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RNA Abundance Analysis by Hailing Jin,Walter Gassmann Pdf

This book examines a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.

MicroRNA Detection and Target Identification

Tamas Dalmay
MicroRNA Detection and Target Identification Book PDF

Author : Tamas Dalmay
Publisher : Springer Nature
Page : 267 pages
File Size : 40,9 Mb
Release : 2023-01-23
Category : Science
ISBN : 9781071629826

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MicroRNA Detection and Target Identification by Tamas Dalmay Pdf

This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

A Laboratory Guide to RNA

Paul A. Krieg
A Laboratory Guide to RNA Book PDF

Author : Paul A. Krieg
Publisher : John Wiley & Sons
Page : 470 pages
File Size : 55,5 Mb
Release : 1996-08-15
Category : Science
ISBN : 0471125369

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A Laboratory Guide to RNA by Paul A. Krieg Pdf

Here is the most complete guide available to the isolation, analysis, and synthesis of RNA. It covers everything researchers and laboratory workers need to know about the study of gene expression via RNA analysis-from the theory behind the methods, to actual problem-solving techniques. Step-by-step protocols are presented for each method. A careful presentation of the experimental formalities of these protocols enables specialists and nonspecialists alike to implement the methods easily in the laboratory. Each protocol is accompanied by the theoretical background underlying the experimental procedure and most chapters contain illustrations of typical results and troubleshooting tips. A Laboratory Guide to RNA offers a straightforward detailed account of experimental procedures, ranging from the isolation of RNA from a variety of cell and tissue types, detection analysis, and quantitation using a range of strategies, to large- and small-scale synthesis of RNA. This unique guide not only covers established procedures such as RNA blotting and nuclease protection, but also the latest protocols for quantitative PCR and differential display. Protocols addressing in situ hybridization are highlighted in an eight-page, full-color section that illustrates the power of the technique for detection of gene expression in tissues and whole organisms. Featuring contributions from leading research laboratories and the biotechnology field, A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis provides all the methods required for RNA analysis. It is the ideal laboratory guide for research scientists, graduate students, and lab personnel who need a solid reference on the analysis of gene expression at the RNA level.

Protocols for Nucleic Acid Analysis by Nonradioactive Probes

Elena Hilario,John F. MacKay
Protocols for Nucleic Acid Analysis by Nonradioactive Probes Book PDF

Author : Elena Hilario,John F. MacKay
Publisher : Springer Science & Business Media
Page : 318 pages
File Size : 55,6 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781597452298

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Protocols for Nucleic Acid Analysis by Nonradioactive Probes by Elena Hilario,John F. MacKay Pdf

Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Molecular Methods for Virus Detection

Danny L. Wiedbrauk,Daniel H. Farkas
Molecular Methods for Virus Detection Book PDF

Author : Danny L. Wiedbrauk,Daniel H. Farkas
Publisher : Elsevier
Page : 405 pages
File Size : 53,5 Mb
Release : 1995-02-08
Category : Science
ISBN : 9780080536897

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Molecular Methods for Virus Detection by Danny L. Wiedbrauk,Daniel H. Farkas Pdf

Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence Includes quality control information crucial in research and clinical laboratories Most chapters are written by the inventors and principal developers of the methodologies Includes color plates, 77 figures, and 18 tables

RNA Methodologies

Bozzano G Luisa
RNA Methodologies Book PDF

Author : Bozzano G Luisa
Publisher : Academic Press
Page : 332 pages
File Size : 49,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323137799

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RNA Methodologies by Bozzano G Luisa Pdf

This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA. * Studies cellular regulation using RNA as a parameter of Gene Expression * Offers RNA isolation strategies * Explains proper handling, storage, and manipulation of RNA

RNA Tagging

Manfred Heinlein
RNA Tagging Book PDF

Author : Manfred Heinlein
Publisher : Humana
Page : 490 pages
File Size : 46,9 Mb
Release : 2021-08-08
Category : Science
ISBN : 1071607146

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RNA Tagging by Manfred Heinlein Pdf

This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.

RNA Methodologies

Robert E. Farrell Jr.
RNA Methodologies Book PDF

Author : Robert E. Farrell Jr.
Publisher : Academic Press
Page : 742 pages
File Size : 43,7 Mb
Release : 2009-08-31
Category : Science
ISBN : 9780080884950

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RNA Methodologies by Robert E. Farrell Jr. Pdf

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center Includes classic and contemporary techniques useful for all labs

RNA Nanotechnology and Therapeutics

Peixuan Guo,Kirill A. Afonin
RNA Nanotechnology and Therapeutics Book PDF

Author : Peixuan Guo,Kirill A. Afonin
Publisher : CRC Press
Page : 644 pages
File Size : 51,9 Mb
Release : 2022-07-18
Category : Medical
ISBN : 9781000552676

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RNA Nanotechnology and Therapeutics by Peixuan Guo,Kirill A. Afonin Pdf

Interest in RNA nanotechnology has increased in recent years as recognition of its potential for applications in nanomedicine has grown. Edited by the world's foremost experts in nanomedicine, this comprehensive, state-of-the-art reference details the latest research developments and challenges in the biophysical and single molecule approaches in RNA nanotechnology. In addition, the text also provides in-depth discussions of RNA structure for nanoparticle construction, RNA computation and modeling, single molecule imaging of RNA, RNA nanoparticle assembly, RNA nanoparticles in therapeutics, immunorecognition of RNA nanomaterials, RNA chemistry for nanoparticle synthesis, and conjugation and labeling. Presents the latest research and discoveries in RNA nanotechnology Features contributions from world-class experts in the field Covers RNA nanoparticles in therapeutics Describes self-assembled RNA nanoparticles

MicroRNA Expression Detection Methods

Zhiguo Wang,Baofeng Yang
MicroRNA Expression Detection Methods Book PDF

Author : Zhiguo Wang,Baofeng Yang
Publisher : Springer
Page : 388 pages
File Size : 42,6 Mb
Release : 2009-12-23
Category : Science
ISBN : 9783642049286

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MicroRNA Expression Detection Methods by Zhiguo Wang,Baofeng Yang Pdf

MicroRNAs (miRNAs), endogenous noncoding regulatory mRNAs of - nucleotides, have rapidly emerged as the central players in gene expression regulation. Owing to their ever-increasing implications in the control of various biological and pathological processes, miRNAs have now been considered novel biomarkers of various human diseases including, cancer, viral disease, cardiov- cular disorders, metabolic disturbances, etc. Particular expression pro?les have been associated with particular pathological states. Expression pro?ling of miR- NAs have therefore become extremely important not only for fundamentalists but also for clinicians. However, the methodologies used for detecting protein-coding mRNAs cannot be directly applied to miRNAs because of their small size. Over the past years, researchers have made great efforts to developing techniques suitable for miRNA detection and quanti?cation; a wide spectrum of creative and inno- tive techniques (more than 30 different methods) have been invented and validated. It has come to the time now to summarize these methods and present them in an orderly manner for better understanding and utilization of these methods to miRNA research and applications. In particular, the development of methods for quantifying circulating miRNAs opens up a fascinating opportunity for realizing miRNA as diagnostic and prognostic biomarkers of human disease. A book on this subject may help boosting up the passion of researchers to further improve the existing techniques and develop more new methods to ?t to new application needs. These considerations prompted us and urged us to undertake the work: writing a book focusing on miRNA expression detection methods.