Microrna Detection And Target Identification

Author : Tamas Dalmay
Publisher : Springer Nature
Page : 267 pages
File Size : 54,8 Mb
Release : 2023-01-23
Category : Science
ISBN : 9781071629826

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MicroRNA Detection and Target Identification by Tamas Dalmay Pdf

This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Author : Alessandro Laganà
Publisher : Unknown
Page : 359 pages
File Size : 53,8 Mb
Release : 2019
Category : MicroRNA
ISBN : 1493992074

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MicroRNA Target Identification by Alessandro Laganà Pdf

Author : Zhiguo Wang,Baofeng Yang
Publisher : Springer
Page : 388 pages
File Size : 49,5 Mb
Release : 2009-12-23
Category : Science
ISBN : 9783642049286

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MicroRNA Expression Detection Methods by Zhiguo Wang,Baofeng Yang Pdf

MicroRNAs (miRNAs), endogenous noncoding regulatory mRNAs of - nucleotides, have rapidly emerged as the central players in gene expression regulation. Owing to their ever-increasing implications in the control of various biological and pathological processes, miRNAs have now been considered novel biomarkers of various human diseases including, cancer, viral disease, cardiov- cular disorders, metabolic disturbances, etc. Particular expression pro?les have been associated with particular pathological states. Expression pro?ling of miR- NAs have therefore become extremely important not only for fundamentalists but also for clinicians. However, the methodologies used for detecting protein-coding mRNAs cannot be directly applied to miRNAs because of their small size. Over the past years, researchers have made great efforts to developing techniques suitable for miRNA detection and quanti?cation; a wide spectrum of creative and inno- tive techniques (more than 30 different methods) have been invented and validated. It has come to the time now to summarize these methods and present them in an orderly manner for better understanding and utilization of these methods to miRNA research and applications. In particular, the development of methods for quantifying circulating miRNAs opens up a fascinating opportunity for realizing miRNA as diagnostic and prognostic biomarkers of human disease. A book on this subject may help boosting up the passion of researchers to further improve the existing techniques and develop more new methods to ?t to new application needs. These considerations prompted us and urged us to undertake the work: writing a book focusing on miRNA expression detection methods.

Author : John J. Rossi
Publisher : Academic Press
Page : 304 pages
File Size : 42,7 Mb
Release : 2007-08-30
Category : Science
ISBN : 0080551394

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MicroRNA Methods by John J. Rossi Pdf

MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression. This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process – or stops it – is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; MicroRNA expression, maturation and functional analysis; and MicroRNAs and disease.

Author : Xueji Zhang,Haifeng Dong,Yaping Tian
Publisher : Springer
Page : 108 pages
File Size : 40,7 Mb
Release : 2015-05-26
Category : Science
ISBN : 9783662472934

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MicroRNA Detection and Pathological Functions by Xueji Zhang,Haifeng Dong,Yaping Tian Pdf

This book summarizes microRNA (miRNA) biology in a variety of pathological processes, emphasizing the significant potential applications of miRNA in diagnostics and prognostics, as well as novel drug targets. The conventional techniques used for miRNA detection including standard PCR, Northern blotting, microarray and clone methods are addressed. Recent emerging strategies in miRNA detection and quantification with superior flexibility and adaptability, such as novel molecular biological techniques and locked nucleic acid (LNA) modified probes, as well as nanotechnology-based approaches, are also included. The book also highlights the latest advances in clinical-related miRNA detection methods in living cells, circulating blood and tissue, such as in situ hybridization (ISH) and molecular imaging techniques, which are useful to elucidate the biogenesis and biological function of miRNAs in vivo. Finally, the respective advantages and drawbacks of various detection techniques in this fast-moving field are discussed, along with the challenges and promising new directions. This book offers a valuable resource for analytical chemists, biologists and physicians involved in miRNA research. Dr. Xueji Zhang and Dr. Haifeng Dong are Professors at the School of Chemistry & Biological Engineering, University of Science & Technology Beijing (USTB), China. Dr. Yaping Tian is a Professor at the Department of Clinical Biochemistry, Chinese PLA General Hospital and Military Medical School, China.

Author : Shao-Yao Ying
Publisher : Humana Press
Page : 375 pages
File Size : 48,7 Mb
Release : 2012-09-26
Category : Medical
ISBN : 1627030840

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MicroRNA Protocols by Shao-Yao Ying Pdf

Extensive studies have been conducted on the identification, biogenesis, and processing of microRNA (miRNA) as well as research on the exact mechanism by which miRNAs bring about translational silencing of their targets. In addition, numerous publications point to an important role of miRNAs in development, reprogramming, epigenetics, pathogenesis of cancer, oncogenes and tumor suppressors, biomarkers of various disease onset, and regulation of adipogenesis and obesity; yet many questions still remain. In MicroRNA Protocols, Second Edition, experts in the field provide up-to-date coverage of this diverse area of study. The specific chapters of this edition are related to the analysis of miRNA, targets and expression profiling, various methods to determine its regulation of gene expression, the preparation and isolation of miRNAs in specific tissues, its detection in the saliva, and potential application in cosmetics, wound healing, and prostate cancer. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and known pitfalls. Fully updated and easy to use, MicroRNA Protocols, Second Edition aims to stimulate readers to explore diverse ways to understand the mechanisms in which miRNAs facilitate the molecular aspects of not only biomedical research but also a wide range of other research fields.

Author : Tamas Dalmay
Publisher : Humana Press
Page : 0 pages
File Size : 49,9 Mb
Release : 2011-03-17
Category : Science
ISBN : 1617790826

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MicroRNAs in Development by Tamas Dalmay Pdf

Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, MicroRNAs in Development: Methods and Protocols serves as a practical guide for scientists of all backgrounds and conveys the appropriate sense of fascination associated with this vital field of research.

Author : Krishnarao Appasani
Publisher : Cambridge University Press
Page : 580 pages
File Size : 53,7 Mb
Release : 2009-08-20
Category : Science
ISBN : 0521118557

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MicroRNAs by Krishnarao Appasani Pdf

MicroRNAs (miRNAs) are RNA molecules, conserved by evolution, that regulate gene expressions and their recent discovery is revolutionising both basic biomedical research and drug discovery. Expression levels of MiRNAs have been found to vary between tissues and with developmental stages and hence evaluation of the global expression of miRNAs potentially provides opportunities to identify regulatory points for many different biological processes. This wide-ranging reference work, written by leading experts from both academia and industry, will be an invaluable resource for all those wishing to use miRNA techniques in their own research, from graduate students, post-docs and researchers in academia to those working in R&D in biotechnology and pharmaceutical companies who need to understand this emerging technology. From the discovery of miRNAs and their functions to their detection and role in disease biology, this volume uniquely integrates the basic science with industry application towards drug validation, diagnostic and therapeutic development. Forewords by: Sidney Altman, Yale University, Winner of the Nobel Prize in Chemistry, 1989 and Victor R. Ambros, Dartmouth Medical School, Co-discoverer of MicroRNAs

Author : Stefan de Folter
Publisher : Humana Press
Page : 363 pages
File Size : 48,7 Mb
Release : 2019-01-31
Category : Science
ISBN : 1493990411

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Plant MicroRNAs by Stefan de Folter Pdf

This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.

Author : Gyana Ranjan Rout,K.V. Peter
Publisher : Academic Press
Page : 664 pages
File Size : 52,5 Mb
Release : 2022-07-16
Category : Science
ISBN : 9780323899130

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Omics in Horticultural Crops by Gyana Ranjan Rout,K.V. Peter Pdf

Omics in Horticulture Crops presents a comprehensive view of germplasm diversity, genetic evolution, genomics, proteomics and transcriptomics of fruit crops (temperate, tropical and subtropical fruits, fruit nuts, berries), vegetables, tuberous crops, ornamental and floricultural crops and medicinal aromatic plants. Information covering phenomics, genetic diversity, phylogenetic studies, genome sequencing, and genome barcoding through the utilization of molecular markers plays an imperative role in the characterization and effective utilization of diverse germplasm are included in the book. This is a valuable reference for researchers and academics seeking to improve cultivar productivity through enhanced genetic diversity while also retaining optimal traits and protecting the growing environment. Highlights perspectives, progress and promises of -omics application Provides a systematic overview of origin, progenitor and domestication process as well as genetic insights Includes full range of horticultural crops

Author : Lesley J. Collins
Publisher : Springer Science & Business Media
Page : 297 pages
File Size : 54,8 Mb
Release : 2011-09-15
Category : Medical
ISBN : 9781461403326

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RNA Infrastructure and Networks by Lesley J. Collins Pdf

RNAs form complexes with proteins and other RNAs. The RNA‐infrastructure represents the spatiotemporal interaction of these proteins and RNAs in a cell‐wide network. RNA Infrastructure and Networks brings together these ideas to illustrate the scope of RNA‐based biology, and how connecting RNA mechanisms is a powerful tool to investigate regulatory pathways. This book is but a taste of the wide range of RNA‐based mechanisms that connect in the RNA infrastructure.

Author : Aryadeep Roychoudhury,Durgesh K. Tripathi
Publisher : John Wiley & Sons
Page : 613 pages
File Size : 42,7 Mb
Release : 2019-06-13
Category : Science
ISBN : 9781119463672

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Molecular Plant Abiotic Stress by Aryadeep Roychoudhury,Durgesh K. Tripathi Pdf

A close examination of current research on abiotic stresses in various plant species The unpredictable environmental stress conditions associated with climate change are significant challenges to global food security, crop productivity, and agricultural sustainability. Rapid population growth and diminishing resources necessitate the development of crops that can adapt to environmental extremities. Although significant advancements have been made in developing plants through improved crop breeding practices and genetic manipulation, further research is necessary to understand how genes and metabolites for stress tolerance are modulated, and how cross-talk and regulators can be tuned to achieve stress tolerance. Molecular Plant Abiotic Stress: Biology and Biotechnology is an extensive investigation of the various forms of abiotic stresses encountered in plants, and susceptibility or tolerance mechanisms found in different plant species. In-depth examination of morphological, anatomical, biochemical, molecular and gene expression levels enables plant scientists to identify the different pathways and signaling cascades involved in stress response. This timely book: Covers a wide range of abiotic stresses in multiple plant species Provides researchers and scientists with transgenic strategies to overcome stress tolerances in several plant species Compiles the most recent research and up-to-date data on stress tolerance Examines both selective breeding and genetic engineering approaches to improving plant stress tolerances Written and edited by prominent scientists and researchers from across the globe Molecular Plant Abiotic Stress: Biology and Biotechnology is a valuable source of information for students, academics, scientists, researchers, and industry professionals in fields including agriculture, botany, molecular biology, biochemistry and biotechnology, and plant physiology.

Author : Massimo Negrini,George A. Calin,Carlo M. Croce
Publisher : Academic Press
Page : 434 pages
File Size : 45,8 Mb
Release : 2022-02-18
Category : Science
ISBN : 9780128232743

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MicroRNA in Human Malignancies by Massimo Negrini,George A. Calin,Carlo M. Croce Pdf

MicroRNA in Human Malignancies offers a deep overview of the role and translational significance of miRNAs in the development of cancer and other malignancies. The book establishes the foundations of the field by covering essential mechanisms and the translational potential of miRNAs in the field of oncology. Specific topics covered include invasion and metastasis, miRNAs and metabolism, and opportunities of miRNAs in therapeutics. Chapters on diseases include content on disease-related pathophysiology, as well as diagnostic, prognostic and predictive value. This book is an essential reference for students entering the field, as well as researchers and investigators. Provides fundamental and translational chapters that facilitate the acquisition of knowledge needed to design and perform innovative miRNA-related research studies Synthesizes current research, with a critical review on the field Offers in-depth research by leading experts in the field

Author : Roland K. Hartmann,Albrecht Bindereif,Astrid Sch¿n,Eric Westhof
Publisher : John Wiley & Sons
Page : 1370 pages
File Size : 41,7 Mb
Release : 2015-06-22
Category : Science
ISBN : 9783527327768

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Handbook of RNA Biochemistry by Roland K. Hartmann,Albrecht Bindereif,Astrid Sch¿n,Eric Westhof Pdf

The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA.

Author : Srinivas Aluru
Publisher : CRC Press
Page : 1108 pages
File Size : 52,7 Mb
Release : 2005-12-21
Category : Computers
ISBN : 9781420036275

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Handbook of Computational Molecular Biology by Srinivas Aluru Pdf

The enormous complexity of biological systems at the molecular level must be answered with powerful computational methods. Computational biology is a young field, but has seen rapid growth and advancement over the past few decades. Surveying the progress made in this multidisciplinary field, the Handbook of Computational Molecular Biology of