Rnai

Author : Gregory J. Hannon
Publisher : CSHL Press
Page : 568 pages
File Size : 46,6 Mb
Release : 2003
Category : Gene silencing
ISBN : 0879696419

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RNAi by Gregory J. Hannon Pdf

In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.

Author : R. K. Gaur,Yedidya Gafni,P. Sharma,V. K. Gupta
Publisher : CRC Press
Page : 380 pages
File Size : 42,6 Mb
Release : 2016-04-19
Category : Science
ISBN : 9781439873250

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RNAi Technology by R. K. Gaur,Yedidya Gafni,P. Sharma,V. K. Gupta Pdf

RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA

Author : Kun Cheng,Ram I. Mahato
Publisher : John Wiley & Sons
Page : 660 pages
File Size : 48,8 Mb
Release : 2013-04-19
Category : Science
ISBN : 9781118610732

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Advanced Delivery and Therapeutic Applications of RNAi by Kun Cheng,Ram I. Mahato Pdf

Commonly used by researchers to develop technologies for modifying and studying genetic process, RNA interference (RNAi) has many potential uses in medicine, biotechnology, and functional genomics. This book covers all essential aspects involved in the development of RNAi therapeutics, providing detailed guidance on the challenges and opportunities of bringing RNAi technologies from bench to clinic. It explores the design and mechanism of RNAi molecules, delivery strategies, and therapeutic applications in various diseases. Preclinical, regulatory, market, and intellectual aspects of RNAi technologies are also covered.

Author : Sailen Barik
Publisher : Humana
Page : 0 pages
File Size : 48,9 Mb
Release : 2011-01-19
Category : Science
ISBN : 1617378194

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RNAi by Sailen Barik Pdf

RNA interference (RNAi) is the most recent discovery to revolutionize the study of biology. In this book, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Cutting edge and clearly written, this book enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field.

Author : Luis M. Vaschetto
Publisher : Unknown
Page : 369 pages
File Size : 41,8 Mb
Release : 2022
Category : Pests
ISBN : 1071616331

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RNAi Strategies for Pest Management by Luis M. Vaschetto Pdf

This volume explores the latest techniques used for sustainable development of eco-friendly pest management systems. Chapters in this book look at the design of novel RNAi-mediated pest control strategies and related issues. Some of the topics covered in this book are identification and characterization of differentially expressed non-coding RNAs in insect genomes; functional small RNA design; analysis of endogenous RNAi pathways; RNAi screening; plant models of RNAi-mediated pest control; and dsRNA delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNAi Strategies for Pest Management: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field. Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : Kamel A Abd-Elsalam,Ki-Taek Lim
Publisher : Elsevier
Page : 842 pages
File Size : 46,6 Mb
Release : 2021-02-27
Category : Technology & Engineering
ISBN : 9780128219119

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CRISPR and RNAi Systems by Kamel A Abd-Elsalam,Ki-Taek Lim Pdf

Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas. CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems. Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection Outlines the major techniques and applications of both CRISPR and RNAi technologies Assesses the major challenges of escalating these technologies on a mass scale

Author : Martin Latterich
Publisher : Taylor & Francis
Page : 154 pages
File Size : 40,8 Mb
Release : 2008-09-04
Category : Science
ISBN : 9781134130870

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RNAi by Martin Latterich Pdf

"A key feature of RNAi is the highlighting of the pitfalls that can occur and how to minimize them. The book also contains a complete list of abbreviations, as there are many confusing terms associated with RNAi."--Jacket

Author : Hailing Jin,Padubidri V. Shivaprasad,Azeddine Si Ammour,András Székács,Michael L. Mendelsohn
Publisher : Frontiers Media SA
Page : 151 pages
File Size : 49,7 Mb
Release : 2021-09-29
Category : Science
ISBN : 9782889714025

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RNAi Based Pesticides by Hailing Jin,Padubidri V. Shivaprasad,Azeddine Si Ammour,András Székács,Michael L. Mendelsohn Pdf

Author : Bruno Mezzetti,Jeremy Bruton Sweet,Guy Smagghe,Elena Baraldi,Salvatore Arpaia,Antje Dietz-Pfeilstetter,Vera Ventura
Publisher : Frontiers Media SA
Page : 168 pages
File Size : 49,6 Mb
Release : 2022-08-04
Category : Science
ISBN : 9782889767175

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Advances and Challenges of RNAi Based Technologies for Plants - Volume 2 by Bruno Mezzetti,Jeremy Bruton Sweet,Guy Smagghe,Elena Baraldi,Salvatore Arpaia,Antje Dietz-Pfeilstetter,Vera Ventura Pdf

Author : Anonim
Publisher : Elsevier
Page : 318 pages
File Size : 42,5 Mb
Release : 2023-11-19
Category : Science
ISBN : 9780323954679

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Insecticidal Proteins and RNAi in the Control of Insects by Anonim Pdf

Insecticidal Proteins and RNAi in the Control of Insects, Volume 65 in the Advances in Insect Physiology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics including Insecticidal RNAi delivered as biopesticide, Cry toxins, Vip3 insecticidal proteins: Structure and Mode of Action, New plant/other bacteria toxins, Engineered insecticidal proteins, Resistance to insecticidal proteins, and Photorhabdus toxins across Gram-negative bacteria. Each chapter is written by an international board of authors. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Advances in Insect Physiology series Updated release includes the latest information on the Insecticidal proteins and RNAi in the control of Insects

Author : Susana Araújo,Pedro Fevereiro,Matthias Fladung,Bruno Mezzetti,Guy Smagghe,Jeremy Bruton Sweet
Publisher : Frontiers Media SA
Page : 103 pages
File Size : 49,5 Mb
Release : 2021-07-28
Category : Science
ISBN : 9782889711000

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Advances and Challenges of RNAi Based Technologies for Plants by Susana Araújo,Pedro Fevereiro,Matthias Fladung,Bruno Mezzetti,Guy Smagghe,Jeremy Bruton Sweet Pdf

Author : John F. Reidhaar-Olson,Cristina M. Rondinone
Publisher : Humana Press
Page : 0 pages
File Size : 47,6 Mb
Release : 2011-12-01
Category : Medical
ISBN : 1617794945

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Therapeutic Applications of RNAi by John F. Reidhaar-Olson,Cristina M. Rondinone Pdf

The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges in this field and revolutionize the treatment of disease.

Author : Ute Schepers
Publisher : John Wiley & Sons
Page : 336 pages
File Size : 40,5 Mb
Release : 2006-03-06
Category : Science
ISBN : 9783527604371

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RNA Interference in Practice by Ute Schepers Pdf

This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn * how RNAi works in worms, flies and mammals, * how to design the most efficient RNAi constructs, * how to achieve transient, stable and conditional RNAi in cell cultures, * how to determine the efficiency of an RNAi experiment, * and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.

Author : Muhammad Sohail
Publisher : CRC Press
Page : 398 pages
File Size : 44,9 Mb
Release : 2004-08-27
Category : Medical
ISBN : 9780203489253

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Gene Silencing by RNA Interference by Muhammad Sohail Pdf

Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by r

Author : Noureddine Benkeblia
Publisher : John Wiley & Sons
Page : 549 pages
File Size : 45,9 Mb
Release : 2017-10-02
Category : Technology & Engineering
ISBN : 9781119079941

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Phytonutritional Improvement of Crops by Noureddine Benkeblia Pdf

An in-depth treatment of cutting-edge work being done internationally to develop new techniques in crop nutritional quality improvement Phytonutritional Improvement of Crops explores recent advances in biotechnological methods for the nutritional enrichment of food crops. Featuring contributions from an international group of experts in the field, it provides cutting-edge information on techniques of immense importance to academic, professional and commercial operations. World population is now estimated to be 7.5 billion people, with an annual growth rate of nearly 1.5%. Clearly, the need to enhance not only the quantity of food produced but its quality has never been greater, especially among less developed nations. Genetic manipulation offers the best prospect for achieving that goal. As many fruit crops provide proven health benefits, research efforts need to be focused on improving the nutritional qualities of fruits and vegetables through increased synthesis of lycopene and beta carotene, anthocyanins and some phenolics known to be strong antioxidants. Despite tremendous growth in the area occurring over the past several decades, the work has only just begun. This book represents an effort to address the urgent need to promote those efforts and to mobilise the tools of biotechnical and genetic engineering of the major food crops. Topics covered include: New applications of RNA-interference and virus induced gene silencing (VIGS) for nutritional genomics in crop plants Biotechnological techniques for enhancing carotenoid in crops and their implications for both human health and sustainable development Progress being made in the enrichment and metabolic profiling of diverse carotenoids in a range of fruit crops, including tomatoes, sweet potatoes and tropical fruits Biotechnologies for boosting the phytonutritional values of key crops, including grapes and sweet potatoes Recent progress in the development of transgenic rice engineered to massively accumulate flavonoids in-seed Phytonutritional Improvement of Crops is an important text/reference that belongs in all universities and research establishments where agriculture, horticulture, biological sciences, and food science and technology are studied, taught and applied.