Separation And Isolation Of Proteins

Author : Rajni Hatti-Kaul,Bo Mattiasson
Publisher : CRC Press
Page : 688 pages
File Size : 46,6 Mb
Release : 2003-02-05
Category : Medical
ISBN : 0203911687

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Isolation and Purification of Proteins by Rajni Hatti-Kaul,Bo Mattiasson Pdf

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.

Author : P. Alexander,R. J. Block
Publisher : Elsevier
Page : 289 pages
File Size : 52,8 Mb
Release : 2014-05-15
Category : Science
ISBN : 9781483149806

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The Separation and Isolation of Proteins by P. Alexander,R. J. Block Pdf

A Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides), Volume 1: The Separation and Isolation of Proteins deals with the techniques used in the separation and isolation of proteins, including fractionation and characterization by dialysis, multi-membrane electrodecantation, and zonal density gradient electrophoresis. The fractionation of proteins by adsorption and ion exchange is also described. This book is comprised of seven chapters and begins with a discussion on procedures for the separation of proteins, paying particular attention to the liberation of proteins from cellular material; removal of lipids from lipoproteins; and denaturation, fractionation, and purification of proteins. The next chapter focuses on the isolation of biologically active proteins such as cytochrome, bacterial amylases, and bacterial proteinases. The reader is methodically introduced to fractionation of proteins by adsorption and ion exchange; fractionation and characterization by dialysis; multi-membrane electrodecantation; and continuous and discontinuous partition. The final chapter explains how zonal density gradient electrophoresis works as a separation method for natural mixtures of proteins, their degradation products, and other substances carrying electric charges in solution or suspension. This volume will be of interest to chemists working with proteins.

Author : Jan-Christer Janson,Lars Rydén
Publisher : John Wiley & Sons
Page : 712 pages
File Size : 54,6 Mb
Release : 1998
Category : Medical
ISBN : 0471186260

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Protein Purification by Jan-Christer Janson,Lars Rydén Pdf

This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.

Author : Robert K. Scopes
Publisher : Springer Science & Business Media
Page : 342 pages
File Size : 54,8 Mb
Release : 2013-04-17
Category : Science
ISBN : 9781475719574

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Protein Purification by Robert K. Scopes Pdf

New textbooks at alllevels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a reallack ofup-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. lt is not often easy to persuade such individuals to settime aside to help spread the knowledge they have accumu lated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses. NewYork CHARLES R. CANTOR Preface to the Second Edition The original plan for the first edition of this book was to title it Enzyme Purification: Princip/es and Practice.

Author : Peter Alexander,Alexander R. J. Block,R. Block
Publisher : Pergamon
Page : 254 pages
File Size : 55,9 Mb
Release : 1960
Category : Electronic
ISBN : 008009256X

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Separation and Isolation of Proteins by Peter Alexander,Alexander R. J. Block,R. Block Pdf

Author : Paul Cutler
Publisher : Springer Science & Business Media
Page : 474 pages
File Size : 47,6 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592596553

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Protein Purification Protocols by Paul Cutler Pdf

The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.

Author : Murray P. Deutscher
Publisher : Gulf Professional Publishing
Page : 936 pages
File Size : 51,6 Mb
Release : 1990
Category : Proteins
ISBN : 0121820831

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Guide to Protein Purification by Murray P. Deutscher Pdf

Guide to Protein Purification , designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure.

Author : Odoardo Jose Brito
Publisher : Unknown
Page : 146 pages
File Size : 48,8 Mb
Release : 1974
Category : Proteins
ISBN : WISC:89014959266

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Procedures for the Separation and Isolation of Protein from Sesame Seed by Odoardo Jose Brito Pdf

Author : Gebhard von Jagow
Publisher : Elsevier
Page : 185 pages
File Size : 48,6 Mb
Release : 2013-10-22
Category : Science
ISBN : 9780080571720

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A Practical Guide to Membrane Protein Purification by Gebhard von Jagow Pdf

A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing. Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use. Provides general guidelines and strategies for isolation of membrane proteins Describes detailed practical procedures that have been the widest applications, and lowest specialized equipment needs Gives special emphasis to new native and denaturing electrophoresis techniques Explains modifications of techniques used for water-soluble proteins

Author : R. D. King
Publisher : Springer Science & Business Media
Page : 328 pages
File Size : 46,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789400934115

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Food Biotechnology—1 by R. D. King Pdf

Biotechnology in its many guises has developed very considerably over the last few years. We now feel that it is appropriate for the publication of a series of books that discuss the technical aspects of biotechnology specifically as applied to foods, and in particular concentrating on new and emerging techniques, processes and products. Food is without doubt one of the oldest bioindustries; however, some of the new areas of biotechnology, such as diagnostic and health-care applications, are likely to mature much faster than applications in the food industry. Eventually, however, biotechnology must have a very great impact on a wide scale in the food industry, simply because of the size and diversity of the industry, and because most food products are substantially natural in origin and are therefore very suitable for processing by biocatalysts. Some of the ways in which the food industry is likely to be affected by developments in biotechnology include the following: The modification of food components to give products with new and/or improved properties, for instance high fructose corn syrups, and by modifying the functional properties of proteins. New methods of assaying food constituents, such as immobilized enzyme sensors. New processes for the production of foods and food components, for instance the use of plant cell cultures for the production of flavours. Many of these topics will be described in detail in this series of books.

Author : Hafiz Ahmed,Hafiz Ahmed PhD
Publisher : CRC Press
Page : 408 pages
File Size : 47,7 Mb
Release : 2017-07-27
Category : Medical
ISBN : 9780203507438

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Principles and Reactions of Protein Extraction, Purification, and Characterization by Hafiz Ahmed,Hafiz Ahmed PhD Pdf

Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different

Author : Daniel R. Marshak
Publisher : CSHL Press
Page : 420 pages
File Size : 48,9 Mb
Release : 1996
Category : Protein engineering
ISBN : 0879693851

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Strategies for Protein Purification and Characterization by Daniel R. Marshak Pdf

Cold Spring Harbor Laboratory. Softcover manual of fundamental procedures commonly used in protein biochemistry, for reseachers. Plastic comb spiral binding.

Author : E. L. V. Harris,S. Angal
Publisher : Oxford University Press, USA
Page : 340 pages
File Size : 46,9 Mb
Release : 1989
Category : Language Arts & Disciplines
ISBN : UCSD:31822026043448

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Protein Purification Methods by E. L. V. Harris,S. Angal Pdf

This book brings together useful practical protocols for the purification of proteins, concentrating on the uses of buffers and different means of separation, by charge, activity and size. Diverse applications of these methods can be found in the companion volume.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 55,8 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Rajni Hatti-Kaul,Bo Mattiasson
Publisher : CRC Press
Page : 653 pages
File Size : 50,6 Mb
Release : 2003-02-05
Category : Science
ISBN : 9780824747596

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Isolation and Purification of Proteins by Rajni Hatti-Kaul,Bo Mattiasson Pdf

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.