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Author : R. Hampton,Ellen M. Ball
Publisher : Amer Phytopathological Society
Page : 389 pages
File Size : 44,8 Mb
Release : 1990
Category : Science
ISBN : 0890541159
Preparative methods; Assay environments: liquid phase; Assay environments: solid phase; New applications or special topics.
Author : P. Narayanasamy
Publisher : CRC Press
Page : 548 pages
File Size : 41,8 Mb
Release : 2001-08-28
Category : Science
ISBN : 0824705912
This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materials and quarantine techniques for screening newly introduced plant materials.
Author : J. M. Duncan
Publisher : Wiley-Blackwell
Page : 235 pages
File Size : 51,9 Mb
Release : 1992
Category : Science
ISBN : 0632030666
Immunological techniques: Serological methods to detect plant viruses:production and use of monoclonal antibodies; Immunodiagnostic techniques for plant mycoplasma-like organisms; Detection of plant-invading fungi by monoclonal antibodies; Assay methods for mycotoxins using monoclonal antibodies; Techniques for detecting and identifying plant pathogenic bacteria. Nucleic Acid Techniques: Application of double-stranded RNA analysis of plant to detect viruses, virus-like agents, virus satellites and subgenomic viral RNAs; Detection of viroids and viruses by nucleic acid probes; Identification of plant pathogenic bacteria using nucleic acid technology; Use of RFLPs to identify races of fungal pathogens. Coomercial aspects and practical applications: Screening forpathogens and contaminating micro-organisms in micropropagation; Application of rapid techniques to seed health testing prospects and potential; From the research bench to the market place: development of commercial diagnostic kits; Commercialization of serological tests for plant viruses.
Author : Moslih Al-Moslih
Publisher : BoD – Books on Demand
Page : 174 pages
File Size : 53,7 Mb
Release : 2012-03-21
Category : Medical
ISBN : 9789535103707
This book explains the concept of serological methods used in laboratory diagnoses of certain bacteria, mycoplasmas, viruses in humans, animals and plants, certain parasitic agents as well as autoimmune disease. The authors present up-to-date information concerning the serological methods in laboratory diagnosis of such infectious diseases. Section one deals with the serological methods for bacteria. Section 2 deals with serological methods in human, animal and plant viruses. Section 3 is concerned with the serological laboratory diagnosis of echinococcus and human toxocariasis agents. The last section deals with serological laboratory methods in the diagnosis of coeliac disease.
Author : P. Narayanasamy
Publisher : Springer Science & Business Media
Page : 341 pages
File Size : 49,7 Mb
Release : 2010-10-26
Category : Science
ISBN : 9789048197545
The need for the development of techniques based on the characteristics of the viral proteins and genomic nucleic acids was realized in order to detect, identify, differentiate and quantify viruses in the infected plants/planting materials with or without symptoms of infection. Immunoassays have been successfully applied for the detection of viruses in crop and weed host plant species as well as in the vectors. Nucleic acid-based techniques have been demonstrated to be the most reliable and sensitive tests for detection, identification and differentiation of viruses and viroids present in plants and planting materials.. Inclusion of numerous protocols in appropriate chapters as appendix is a unique feature of this volume.
Author : H.-W. Dehne,G. Adam,M. Diekmann,J. Frahm,A. Mauler-Machnik,P. van Halteren
Publisher : Springer Science & Business Media
Page : 501 pages
File Size : 40,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789400900431
The diagnosis and identification of plant pathogens provides the basis of plant pathology and phytomedicine. The Executive Committee of the EUROPEAN FOUNDATION FOR PLANT PATHOLOGY (EFPP) had no problem to identify this actual th topic as topic for the 4th Symposium, which was held from September 8 to the 12th at the University of Bonn. It was suggested to have introductory papers and papers on actual research on recently identified topics. The development of diagnosis and pathogen identification is very important to keep plants healthy and to provide a successful and efficient disease control. On the other hand the most important task of the EUROPEAN FOUNDATION FOR PLANT PATHOLOGY is to improve the international communication, especially in the European hemisphere. Another important duty is to provide the contact between all associated societies - of specific importance seems to be the contact to societies and colleagues from eastern European countries. Times have changed and gratefully we are obliged to hold the contact to our colleagues from the east. During the last meeting we could hold this contact to a certain extent and this should be a premise for the future. th During 1998 the EUROPEAN FOUNDATION FOR PLANT PATHOLOGY will join the 7 International Congress of Plant Pathology held at Edinburgh from August 9-14, 1998. th The 5 Symposium of the EUROPEAN FOUNDATION FOR PLANT PATHOLOGY will be arranged by our Italian colleagues.
Author : Baozhong Meng,Giovanni P. Martelli,Deborah A. Golino,Marc Fuchs
Publisher : Springer
Page : 698 pages
File Size : 49,9 Mb
Release : 2017-07-05
Category : Science
ISBN : 9783319577067
The domestication of grapes dates back five thousand years ago and has spread to nearly all continents. In recent years, grape acreage has increased dramatically in new regions, including the United States of America, Chile, Asia (China and India), and Turkey. A major limiting factor to the sustained production of premium grapes and wines is infections by viruses. The advent of powerful molecular and metagenomics technologies, such as molecular cloning and next generation sequencing, allowed the discovery of new viruses from grapes. To date, grapevine is susceptible to 64 viruses that belong to highly diverse taxonomic groups. The most damaging diseases include: (1) infectious degeneration; (2) leafroll disease complex; and (3) rugose wood complex. Recently, two new disease syndromes have been recognized: Syrah decline and red blotch. Losses due to fanleaf degeneration are estimated at $1 billion annually in France alone. Other diseases including leafroll, rugose wood, Syrah de cline and red blotch can result in total crop loss several years post-infection. This situation is further exacerbated by mixed infections with multiple viruses and other biotic as well as adverse abiotic environmental conditions, such as drought and winter damage, causing even greater destruction. The book builds upon the last handbook (written over twenty years ago) on the part of diagnostics and extensively expands its scope by inclusion of molecular biology aspects of select viruses that are widespread and economically most important. This includes most current information on the biology, transmission, genome replication, transcription, subcellular localization, as well as virus-host interactions. It also touches on several novel areas of scientific inquiry. It also contains suggested directions for future research in the field of grapevine virology.
Author : Oluf Gamborg,Gregory C. Phillips
Publisher : Springer Science & Business Media
Page : 367 pages
File Size : 46,9 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783642790485
This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.
Author : U. S. Singh,Rudra P. Singh
Publisher : CRC Press
Page : 836 pages
File Size : 41,7 Mb
Release : 2017-12-14
Category : Science
ISBN : 9781351430210
Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.
Author : Sven Erik Albrechtsen
Publisher : CABI
Page : 280 pages
File Size : 48,7 Mb
Release : 2006
Category : Science
ISBN : 9780851990163
This book provides a practical guide to the commonly used detection methods for seed-transmitted viruses and viroids affecting both tropical and non-tropical crops. The first part describes important aspects of seed-transmitted viral diseases. The second and main part contains principles of the detection techniques and step-by-step protocols accompanied by method optimization and comments. Most of the described techniques can be equally applied to plant viruses and viroids other than seedborne ones. This book will be of significant interest to those working in seed testing laboratories and students and teachers within plant pathology and seed science.
Author : James B. Sinclair,Onkar Dev Dhingra
Publisher : CRC Press
Page : 449 pages
File Size : 49,9 Mb
Release : 2017-11-22
Category : Science
ISBN : 9781351464512
The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations. The Second Edition includes chapters on the following: Sterilization of culture apparatus and culture media Culture of pathogens with detailed techniques for 61 fungi and selected bacteria Long-term storage of plant pathogens Detection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera-15 methods for airborne inoculum and 13 methods for seedborne pathogens Establishment of disease and testing for disease resistance Work with soil microorganisms Fungicide evaluation Biological control Bright-field microscopy
Author : Gad Loebenstein,P.H. Berger,A.A. Brunt,R.H. Lawson
Publisher : Springer Science & Business Media
Page : 479 pages
File Size : 55,6 Mb
Release : 2013-12-01
Category : Science
ISBN : 9789400708426
This is a comprehensive up-to-date treatise including information on virus-, viroid-, and phytoplasma-induced potato diseases. The chapters of this book were written by internationally well-known experts and include novel techniques of detection, virus isolation, transmission, and epidemiology of the pathogens.
Author : Jeanne Dijkstra,Cees de Jager
Publisher : Springer Science & Business Media
Page : 451 pages
File Size : 50,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642720307
Viruses require a special approach to establish their presence in a diseased plant since they are not visible, even under a light microscope. This manual describes in detail a variety of protocols for determining the properties and identity of a virus and its behavior in infected plants. A Springer Lab Manual.
Author : Krishna P. Singh,Shamarao Jahagirdar,Birinchi Kumar Sarma
Publisher : Springer Nature
Page : 849 pages
File Size : 40,8 Mb
Release : 2020-12-09
Category : Science
ISBN : 9789811562754
This book offers a comprehensive guide to the identification, detection, characterization, classification and management of plant pathogens and other beneficial microbes in agriculture. The science of plant pathology is a dynamic field and, given the growing interest in sustainable agricultural practices, plant disease management has also gained importance. Further, there has been a shift from traditional chemical-based methods to eco-friendly integrated disease management strategies with a greater focus on bio-control and other eco-friendly technologies. This book provides a comprehensive and timely account of latest concepts and advances in the field of plant pathology, including detection and diagnosis, host resistance, disease forecasting and plant biotechnological approaches. Accordingly, it will be of great interest to academics and all stakeholders working in the fields of plant pathology, microbiology, biotechnology, plant breeding, and other life sciences.
Author : R. E. F. Matthews
Publisher : CRC Press
Page : 392 pages
File Size : 41,9 Mb
Release : 2019-07-23
Category : Science
ISBN : 9781351079808
Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.