Single Channel Recording

Author : Bert Sakmann
Publisher : Springer Science & Business Media
Page : 506 pages
File Size : 48,6 Mb
Release : 2013-11-11
Category : Medical
ISBN : 9781461578581

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Single-Channel Recording by Bert Sakmann Pdf

Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals. In the summer of 1982, a course on the technique was held at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. It brought together people from most of the laboratories involved in patch clamping at that time. During the course, it became apparent that the technique had reached a state of maturity. Repeatedly, the opinion was expressed that a detailed description of all the aspects of the technique including representative examples of results should be available. We therefore asked the course instructors, as well as several other colleagues, to provide chapters on selected topics in order to produce this volume. The different variants of patch clamping were described quite extensively in an article by Hamill, Marty, Neher, Sakmann, and Sigworth (Pflugers Archiv 391:85) in 1981. Rather than repeating this survey in an introductory chapter, we chose to reprint that article in the Appendix of this volume (by permission of Springer-Verlag). The methods section will, therefore, go straight into detailed aspects of the technol ogy.

Author : Wolfgang Walz,Alan A. Boulton,Glen B. Baker
Publisher : Springer Science & Business Media
Page : 349 pages
File Size : 49,9 Mb
Release : 2008-02-06
Category : Medical
ISBN : 9781592592760

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Patch-Clamp Analysis by Wolfgang Walz,Alan A. Boulton,Glen B. Baker Pdf

Patch-Clamp Analysis: Advanced Techniques describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.

Author : Bert Sakmann
Publisher : Unknown
Page : 700 pages
File Size : 41,8 Mb
Release : 1995
Category : Ion channels
ISBN : OCLC:31971363

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Single-channel Recording by Bert Sakmann Pdf

Second edition of a reference of techniques in the use of channels and pipettes in the patch clamping process. Includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.

Author : Jie Zheng,Matthew C. Trudeau
Publisher : CRC Press
Page : 682 pages
File Size : 53,5 Mb
Release : 2015-02-25
Category : Medical
ISBN : 9781466551428

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Handbook of Ion Channels by Jie Zheng,Matthew C. Trudeau Pdf

The New Benchmark for Understanding the Latest Developments of Ion ChannelsIon channels control the electrical properties of neurons and cardiac cells, mediate the detection and response to sensory stimuli, and regulate the response to physical stimuli. They can often interact with the cellular environment due to their location at the surface of ce

Author : Juliusz Ashot Kozak,James W. Putney, Jr.
Publisher : CRC Press
Page : 343 pages
File Size : 44,9 Mb
Release : 2017-07-14
Category : Science
ISBN : 9781498752732

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Calcium Entry Channels in Non-Excitable Cells by Juliusz Ashot Kozak,James W. Putney, Jr. Pdf

Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.

Author : Margarida D. Amaral,Karl Kunzelmann
Publisher : Humana Press
Page : 0 pages
File Size : 40,8 Mb
Release : 2011-05-25
Category : Science
ISBN : 1617791164

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Cystic Fibrosis by Margarida D. Amaral,Karl Kunzelmann Pdf

Despite the many milestones in cystic fibrosis (CF) research, progress towards curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature. Cystic Fibrosis: Diagnosis and Protocols aims to provide the CF research community and related researchers with a very wide range of high-quality experimental tools, as an easy way to grasp and use classical and novel methods applied to cystic fibrosis. Volume I: Approaches to Study and Correct CFTR Defects focuses on the cystic fibrosis transmembrane conductance regulator (CFTR) and its expression, biogenesis, structure, and function in terms of the defects causing CF. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Cystic Fibrosis: Diagnosis and Protocols will provide readers with optimal working tools to address pressing questions in the best technical way, while helping all of us, as a research and clinical community, to move faster hand-in-hand toward unravelling the secrets of this challenging disorder and cure it.

Author : Yasunobu Okada
Publisher : Springer
Page : 0 pages
File Size : 47,5 Mb
Release : 2012-04-27
Category : Medical
ISBN : 4431539921

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Patch Clamp Techniques by Yasunobu Okada Pdf

Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.

Author : Nikita Gamper
Publisher : Humana Press
Page : 456 pages
File Size : 50,9 Mb
Release : 2016-05-01
Category : Science
ISBN : 1493960091

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Ion Channels by Nikita Gamper Pdf

This Methods in Molecular Biology book offers strategies and protocols for studying a large group of proteins that form ionic channels in the plasma membrane and intracellular membranes of cells. Includes step-by-step protocols, materials lists, tips and more."

Author : Wolfgang B. Liedtke, MD, PH.D.
Publisher : CRC Press
Page : 467 pages
File Size : 49,6 Mb
Release : 2006-09-29
Category : Science
ISBN : 9781420005844

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TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades by Wolfgang B. Liedtke, MD, PH.D. Pdf

Since the first TRP ion channel was discovered in Drosophila melanogaster in 1989, the progress made in this area of signaling research has yielded findings that offer the potential to dramatically impact human health and wellness. Involved in gateway activity for all five of our senses, TRP channels have been shown to respond to a wide range of stimuli from both within and outside the cell body. How we sense heat and cold, how we taste food, how eggs are fertilized, how the heart expands and contracts is each dependent on the function of these channels. While no single book could possibly cover all the research being undertaken, TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades presents the most advanced compilation of work in this area to date. All 31 chapters are written by international pioneers working at the vanguard of TRP ion channel research. They explain much about the pivotal function and behavior of these channels, which are most exquisitely tuned to their specific tasks, and delve into how researchers are putting this knowledge to use in the development of novel pharmaceuticals, which may well prove effective in ameliorating treatment-resistant conditions including cancer, heart disease, inflammation, and immune system dysfunctions. Individual chapters shed light on selected topics of interest in the TRP arena, such as signal transduction in axonal path-finding, and in vascular, renal, and auditory functions, as well as pain. The text also covers subjects as diverse as mating and fertilization, inflammatory pain, and mechanisms of pheromone detection in mammals. While the book presents much new insight and explores findings that will be of interest to those involved with advanced research, it also includes significant background material for those looking to familiarize themselves with this exceptionally promising path of inquiry.

Author : Anonim
Publisher : Unknown
Page : 1056 pages
File Size : 43,5 Mb
Release : 1958
Category : Aeronautics
ISBN : UFL:31262094015996

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Flight and Aircraft Engineer by Anonim Pdf

Author : Areles Molleman
Publisher : John Wiley & Sons
Page : 186 pages
File Size : 48,9 Mb
Release : 2003-06-13
Category : Science
ISBN : 9780470856512

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Patch Clamping by Areles Molleman Pdf

Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 54,8 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Romain Brette,Alain Destexhe
Publisher : Cambridge University Press
Page : 493 pages
File Size : 45,9 Mb
Release : 2012-09-06
Category : Mathematics
ISBN : 9780521516228

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Handbook of Neural Activity Measurement by Romain Brette,Alain Destexhe Pdf

Underlying principles of the various techniques are explained, enabling neuroscientists to extract meaningful information from their measurements.

Author : Karin C. Margot
Publisher : Unknown
Page : 164 pages
File Size : 46,7 Mb
Release : 1998
Category : Electronic
ISBN : CORNELL:31924084733934

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Single Channel Characterization of a Human GluR3-type Glutamate Receptor-channel by Karin C. Margot Pdf

Author : Mark Dallas,Damian Bell
Publisher : Humana
Page : 333 pages
File Size : 48,6 Mb
Release : 2021-11-13
Category : Medical
ISBN : 1071608207

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Patch Clamp Electrophysiology by Mark Dallas,Damian Bell Pdf

This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.