Stem Cell Culture

Author : Jennie P. Mather
Publisher : Academic Press
Page : 407 pages
File Size : 50,5 Mb
Release : 2011-09-02
Category : Science
ISBN : 9780080878041

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Stem Cell Culture by Jennie P. Mather Pdf

The purpose of Stem Cell Culture is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture. Describes techniques in stem cell research Delineates critical steps and potential pitfalls for each method Covers specific procedures in dealing with Human Embryonic Stem Cells

Author : Institute of Medicine,Board on Neuroscience and Behavioral Health,National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Committee on the Biological and Biomedical Applications of Stem Cell Research
Publisher : National Academies Press
Page : 112 pages
File Size : 48,8 Mb
Release : 2002-01-25
Category : Science
ISBN : 9780309170420

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Stem Cells and the Future of Regenerative Medicine by Institute of Medicine,Board on Neuroscience and Behavioral Health,National Research Council,Division on Earth and Life Studies,Board on Life Sciences,Committee on the Biological and Biomedical Applications of Stem Cell Research Pdf

Recent scientific breakthroughs, celebrity patient advocates, and conflicting religious beliefs have come together to bring the state of stem cell researchâ€"specifically embryonic stem cell researchâ€"into the political crosshairs. President Bush's watershed policy statement allows federal funding for embryonic stem cell research but only on a limited number of stem cell lines. Millions of Americans could be affected by the continuing political debate among policymakers and the public. Stem Cells and the Future of Regenerative Medicine provides a deeper exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of undifferentiated human cells. In terms accessible to lay readers, the book summarizes what we know about adult and embryonic stem cells and discusses how to go about the transition from mouse studies to research that has therapeutic implications for people. Perhaps most important, Stem Cells and the Future of Regenerative Medicine also provides an overview of the moral and ethical problems that arise from the use of embryonic stem cells. This timely book compares the impact of public and private research funding and discusses approaches to appropriate research oversight. Based on the insights of leading scientists, ethicists, and other authorities, the book offers authoritative recommendations regarding the use of existing stem cell lines versus new lines in research, the important role of the federal government in this field of research, and other fundamental issues.

Author : Lyn Healy,Ludmila Ruban
Publisher : Springer
Page : 216 pages
File Size : 50,5 Mb
Release : 2014-11-07
Category : Science
ISBN : 9781489975072

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Atlas of Human Pluripotent Stem Cells in Culture by Lyn Healy,Ludmila Ruban Pdf

This lavishly-illustrated, authoritative atlas explores the intricate art of culturing human pluripotent stem cells. Twelve chapters – containing more than 280 color illustrations – cover a variety of topics in pluripotent stem cell culturing including mouse and human fibroblasts, human embryonic stem cells and induced pluripotent stem cells, characteristic staining patterns, and abnormal cultures, among others. Atlas of Human Pluripotent Stem Cells in Culture is a comprehensive collection of illustrated techniques complemented by informative and educational captions examining what good quality cells look like and how they behave in various environments. Examples of perfect cultures are compared side-by-side to less-than-perfect and unacceptable examples of human embryonic and induced pluripotent stem cell colonies. This detailed and thorough atlas is an invaluable resource for researchers, teachers, and students who are interested in or working with stem cell culturing.

Author : R. Ian Freshney,Glyn N. Stacey,Jonathan M. Auerbach
Publisher : John Wiley & Sons
Page : 360 pages
File Size : 45,5 Mb
Release : 2007-07-16
Category : Science
ISBN : 0470167513

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Culture of Human Stem Cells by R. Ian Freshney,Glyn N. Stacey,Jonathan M. Auerbach Pdf

This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

Author : Joni H. Ylostalo
Publisher : MDPI
Page : 186 pages
File Size : 55,7 Mb
Release : 2021-01-07
Category : Science
ISBN : 9783039438037

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3D Stem Cell Culture by Joni H. Ylostalo Pdf

Recently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications, which may be attainable through 3D culture of stem cells. The 3D cultures mimic the conditions of the natural environment of stem cells better, as cells in 3D cultures exhibit many unique and desirable characteristics that could be beneficial for therapeutic interventions. 3D stem cell cultures may employ supporting structures, such as various matrices or scaffolds, in addition to stem cells, to support complex structures. This book brings together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications.

Author : Uma Lakshmipathy,Jonathan D. Chesnut,Bhaskar Thyagarajan
Publisher : John Wiley & Sons
Page : 512 pages
File Size : 46,6 Mb
Release : 2009-04-06
Category : Medical
ISBN : 0470454911

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Emerging Technology Platforms for Stem Cells by Uma Lakshmipathy,Jonathan D. Chesnut,Bhaskar Thyagarajan Pdf

This book focuses on practical applications for using adult and embryonic stem cells in the pharmaceutical development process. It emphasizes new technologies to help overcome the bottlenecks in developing stem cells as therapeutic agents. A key reference for professionals working in stem cell science, it presents the general principles and methodologies in stem cell research and covers topics such as derivitization and characterization of stem cells, stem cell culture and maintenance, stem cell engineering, applications of high-throughput screening, and stem cell genetic modification with their use for drug delivery.

Author : Amita Sarkar
Publisher : Discovery Publishing House
Page : 310 pages
File Size : 55,7 Mb
Release : 2009
Category : Mesenchymal stem cells
ISBN : 8183564003

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Stem Cell Culture by Amita Sarkar Pdf

Author : Joni H. Ylostalo
Publisher : Unknown
Page : 186 pages
File Size : 49,5 Mb
Release : 2021
Category : Electronic
ISBN : 3039438042

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3D Stem Cell Culture by Joni H. Ylostalo Pdf

Recently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications, which may be attainable through 3D culture of stem cells. The 3D cultures mimic the conditions of the natural environment of stem cells better, as cells in 3D cultures exhibit many unique and desirable characteristics that could be beneficial for therapeutic interventions. 3D stem cell cultures may employ supporting structures, such as various matrices or scaffolds, in addition to stem cells, to support complex structures. This book brings together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications.

Author : Jonathan M. W. Slack
Publisher : John Wiley & Sons
Page : 290 pages
File Size : 47,5 Mb
Release : 2018-01-16
Category : Science
ISBN : 9781119235156

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The Science of Stem Cells by Jonathan M. W. Slack Pdf

Introduces all of the essential cell biology and developmental biology background for the study of stem cells This book gives you all the important information you need to become a stem cell scientist. It covers the characterization of cells, genetic techniques for modifying cells and organisms, tissue culture technology, transplantation immunology, properties of pluripotent and tissue specific stem cells and, in particular, the relevant aspects of mammalian developmental biology. It dispels many misconceptions about stem cells—especially that they can be miracle cells that can cure all ills. The book puts emphasis on stem cell behavior in its biological context and on how to study it. Throughout, the approach is simple, direct, and logical, and evidence is given to support conclusions. Stem cell biology has huge potential for advancing therapies for many distressing and recalcitrant diseases, and its potential will be realized most quickly when as many people as possible have a good grounding in the science of stem cells. Content focused on the basic science underpinning stem cell biology Covers techniques of studying cell properties and cell lineage in vivo and in vitro Explains the basics of embryonic development and cell differentiation, as well as the essential cell biology processes of signaling, gene expression, and cell division Includes instructor resources such as further reading and figures for downloading Offers an online supplement summarizing current clinical applications of stem cells Written by a prominent leader in the field, The Science of Stem Cells is an ideal course book for advanced undergraduates or graduate students studying stem cell biology, regenerative medicine, tissue engineering, and other topics of science and biology.

Author : Jennie P. Mather
Publisher : Unknown
Page : 128 pages
File Size : 50,7 Mb
Release : 2008
Category : Electronic
ISBN : OCLC:1027939276

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Stem Cell Culture (9780123738769). by Jennie P. Mather Pdf

Author : Suzanne Peterson,Jeanne F. Loring
Publisher : Academic Press
Page : 656 pages
File Size : 42,7 Mb
Release : 2012-10-22
Category : Science
ISBN : 9780123854742

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Human Stem Cell Manual by Suzanne Peterson,Jeanne F. Loring Pdf

This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work. Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications. Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America. Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs.

Author : Gary S. Stein,Maria Borowski,Mai X. Luong,Meng-Jiao Shi,Kelly P. Smith,Priscilla Vazquez
Publisher : John Wiley & Sons
Page : 439 pages
File Size : 43,6 Mb
Release : 2011-03-04
Category : Science
ISBN : 9780470889893

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Human Stem Cell Technology and Biology by Gary S. Stein,Maria Borowski,Mai X. Luong,Meng-Jiao Shi,Kelly P. Smith,Priscilla Vazquez Pdf

Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology Perspectives written by the world leaders in the field Introductory chapters will provide background information The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.

Author : Yi-Chen Ethan Li,I-Chi Lee
Publisher : CRC Press
Page : 346 pages
File Size : 52,7 Mb
Release : 2023-12-01
Category : Science
ISBN : 9781003822745

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Stem Cell-based Biosystems by Yi-Chen Ethan Li,I-Chi Lee Pdf

Stem cell science has emerged as a novel discipline in biomedical research over the past decade. With advancements in this field, stem cells have demonstrated versatile applications ranging from basic research to clinical case studies. Recognizing the demand for knowledge in stem cell applications, this book aims to provide comprehensive information on stem cell technology and its application in biosystems. It covers fundamental culture methods, advanced stem cell-based biosystems, and clinical case studies of stem cell therapy. This book is intended for individuals with an interest in stem cell science, offering valuable insights into this rapidly evolving field.

Author : John R. Masters,Bernhard O. Palsson,James A. Thomson
Publisher : Springer Science & Business Media
Page : 285 pages
File Size : 43,6 Mb
Release : 2007-05-20
Category : Medical
ISBN : 9781402059834

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Embryonic Stem Cells by John R. Masters,Bernhard O. Palsson,James A. Thomson Pdf

If you wish to grow or characterize embryonic stem cells or persuade them to differentiate into a particular cell type, then this book contains information that is vital to your success. The aim is to provide clear simple instructions and protocols for growing, maintaining and characterizing embryonic stem cells and details of the various methods used to make stem cells differentiate into specific cell types.

Author : Henning Ulrich,Priscilla Davidson Negraes
Publisher : Springer
Page : 374 pages
File Size : 53,8 Mb
Release : 2016-08-04
Category : Science
ISBN : 9783319305820

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Working with Stem Cells by Henning Ulrich,Priscilla Davidson Negraes Pdf

The content covered encompass not only the theoretic and methodological aspects for culturing human pluripotent stem cells, but also the establishment of induced pluripotent stem cells and their differentiation into specific cellular subtypes. This book has the most updated methods and technologies in an accessible and clear language, presenting protocols developed and optimized by researchers that use them every day. Stem cell research is a growing field that offers the potential to comprehend mechanisms of neural development and tissue regeneration and maintenance as well as the development-related human diseases and the establishment of novel therapies. Questions of basic biology can also be exploited by this cutting-edge approach.