Synthetic Biology Of Yeasts

Author : Farshad Darvishi Harzevili
Publisher : Springer Nature
Page : 377 pages
File Size : 50,5 Mb
Release : 2022-01-21
Category : Science
ISBN : 9783030896805

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Synthetic Biology of Yeasts by Farshad Darvishi Harzevili Pdf

This book covers recent advances and future trends in yeast synthetic biology, providing readers with an overview of computational and engineering tools, and giving insight on important applications. Yeasts are one of the most attractive microbial cell factories for the production of a wide range of valuable products, including pharmaceuticals, nutraceuticals, cosmetics, agrochemicals and biofuels. Synthetic biology tools have been developed to improve the metabolic engineering of yeasts in a faster and more reliable manner. Today, these tools are used to make synthetic pathways and rewiring metabolism even more efficient, producing products at high titer, rate, and yield. Split into two parts, the book opens with an introduction to rational metabolic pathway prediction and design using computational tools and their applications for yeast systems and synthetic biology. Then, it focuses on the construction and assembly of standardized biobricks for synthetic pathway engineering in yeasts, yeast cell engineering and whole cell yeast-based biosensors. The second part covers applications of synthetic biology to produce diverse and attractive products by some well-known yeasts. Given its interdisciplinary scope, the book offers a valuable asset for students, researchers and engineers working in biotechnology, applied microbiology, metabolic engineer ing and synthetic biology.

Author : Farshad Darvishi,Mark Blenner,Rodrigo Ledesma-Amaro
Publisher : Frontiers Media SA
Page : 137 pages
File Size : 47,7 Mb
Release : 2021-10-22
Category : Science
ISBN : 9782889713240

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Synthetic Biology of Yeasts for the Production of Non-Native Chemicals, 2nd Edition by Farshad Darvishi,Mark Blenner,Rodrigo Ledesma-Amaro Pdf

Author : Juan I. Castrillo,Stephen G. Oliver
Publisher : Humana Press
Page : 0 pages
File Size : 48,5 Mb
Release : 2011-08-23
Category : Science
ISBN : 1617791725

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Yeast Systems Biology by Juan I. Castrillo,Stephen G. Oliver Pdf

Systems Biology aims at deciphering the genotype-phenotype relationships at the levels of genes, transcripts (RNAs), peptides, proteins, metabolites, and environmental factors participating in complex cellular networks in order to reveal the mechanisms and principles governing the behavior of complex biological systems. Yeast Systems Biology: Methods and Protocols presents an up-to-date view of the optimal characteristics of the yeast Saccharomyces cerevisiae as a model eukaryote, perspective on the latest experimental and computational techniques for systems biology studies, most of which were first designed for and validated in yeast, and selected examples of yeast systems biology studies and their applications in biotechnology and medicine. These experiments under controlled conditions can uncover the complexity and interplay of biological networks with their dynamics, basic principles of internal organization, and balanced orchestrated functions between organelles in direct interaction with the environment as well as the characterization of short and long-term effects of perturbations and dysregulation of networks that may illuminate the origin of complex human diseases. Written for the highly successful Methods in Molecular BiologyTM series, this volume contains the kind of detailed description and implementation advice that is crucial for getting optimal results. Practical and cutting-edge, Yeast Systems Biology: Methods and Protocols serves researchers interested in comprehensive systems biology strategies in well-defined model systems with specific objectives as well as a better knowledge of the latest post-genomic strategies at all ‘omic levels and computational approaches towards analysis, integration, and modeling of biological systems, from single-celled organisms to higher eukaryotes.

Author : Yongjun Wei,Boyang Ji,Xiao-Jun Ji,Tao Chen,Rodrigo Ledesma-Amaro
Publisher : Frontiers Media SA
Page : 90 pages
File Size : 47,6 Mb
Release : 2022-01-20
Category : Science
ISBN : 9782889741090

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Engineering Yeast to Produce Plant Natural Products by Yongjun Wei,Boyang Ji,Xiao-Jun Ji,Tao Chen,Rodrigo Ledesma-Amaro Pdf

Author : Valeria Mapelli,Maurizio Bettiga
Publisher : Springer Nature
Page : 300 pages
File Size : 42,9 Mb
Release : 2022-07-04
Category : Science
ISBN : 9781071623992

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Yeast Metabolic Engineering by Valeria Mapelli,Maurizio Bettiga Pdf

This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.

Author : Henri Heslot
Publisher : CRC Press
Page : 818 pages
File Size : 43,8 Mb
Release : 2018-01-18
Category : Technology & Engineering
ISBN : 9781351091633

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Molecular Biology and Genetic Engineering of Yeasts by Henri Heslot Pdf

Molecular Biology and Genetic Engineering of Yeasts presents a comprehensive examination of how yeasts are used in genetic engineering. The book discusses baker's yeast, in addition to a number of unconventional yeasts being used in an increasing number of studies. 175 figures help illustrate the information presented. Topics discussed include yeast transformation, yeast plasmids, protein localization and processing in yeast, protein secretion, various aspects of Saccharomyces cerevisiae, and heterologous expression and secretion.

Author : Anonim
Publisher : Gulf Professional Publishing
Page : 961 pages
File Size : 53,9 Mb
Release : 2004-05-14
Category : Science
ISBN : 9780080575131

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Guide to Yeast Genetics and Molecular Biology by Anonim Pdf

Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein

Author : J.F.T. Spencer,D.M. Spencer,A.R.W. Smith
Publisher : Springer Science & Business Media
Page : 546 pages
File Size : 51,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781461254911

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Yeast Genetics by J.F.T. Spencer,D.M. Spencer,A.R.W. Smith Pdf

During the past few decades we have witnessed an era of remarkable growth in the field of molecular biology. In 1950 very little was known of the chemical constitution of biological systems, the manner in which information was trans mitted from one organism to another, or the extent to which the chemical basis of life is unified. The picture today is dramatically different. We have an almost bewildering variety of information detailing many different aspects of life at the molecular level. There great advances have brought with them some breath-taking insights into the molecular mechanisms used by nature for rep licating, distributing and modifying biological information. We have learned a great deal about the chemical and physical nature of the macromolecular nucleic acids and proteins, and the manner in which carbohydrates, lipids and smaller molecules work together to provide the molecular setting of living sys tems. It might be said that these few decades have replaced a near vacuum of information with a very large surplus. It is in the context of this flood of information that this series of monographs on molecular biology has been organized. The idea is to bring together in one place, between the covers of one book, a concise assessment of the state of the subject in a well-defined field. This will enable the reader to get a sense of historical perspectiv(}-what is known about the field today-and a description of the frontiers of research where our knowledge is increasing steadily.

Author : Horst Feldmann
Publisher : John Wiley & Sons
Page : 1 pages
File Size : 41,5 Mb
Release : 2012-09-06
Category : Science
ISBN : 9783527659197

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Yeast by Horst Feldmann Pdf

Finally, a stand-alone, all-inclusive textbook on yeast biology. Based on the feedback resulting from his highly successful monograph, Horst Feldmann has totally rewritten he contents to produce a comprehensive, student-friendly textbook on the topic. The scope has been widened, with almost double the content so as to include all aspects of yeast biology, from genetics via cell biology right up to biotechnology applications. The cell and molecular biology sections have been vastly expanded, while information on other yeast species has been added, with contributions from additional authors. Naturally, the illustrations are in full color throughout, and the book is backed by a complimentary website. The resulting textbook caters to the needs of an increasing number of students in biomedical research, cell and molecular biology, microbiology and biotechnology who end up using yeast as an important tool or model organism.

Author : Ronnie G. Willaert
Publisher : MDPI
Page : 217 pages
File Size : 52,9 Mb
Release : 2019-01-10
Category : Biology (General)
ISBN : 9783038974314

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Yeast Biotechnology 2.0 by Ronnie G. Willaert Pdf

This book is a printed edition of the Special Issue "Yeast Biotechnology 2.0" that was published in Fermentation

Author : John F.T. Spencer,Dorothy M. Spencer
Publisher : Springer Science & Business Media
Page : 389 pages
File Size : 40,8 Mb
Release : 2013-03-09
Category : Science
ISBN : 9783662033708

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Yeasts in Natural and Artificial Habitats by John F.T. Spencer,Dorothy M. Spencer Pdf

A Guide to the World of the Yeasts J. F. T. Spencer and D. M. Spencert As the well-known authority on yeasts, the late Professor Rose, frequently pointed out, it is impossible for one person to present, in a single volume, the details of the life, composiotion, habitats, relationships, and actual and potential uses to man kind of the 500 (at last count) known species of yeasts. This book confirms the truth of this statement. However, our aim is actually more modest than that, and this book is an attempt to introduce the general reader, and possibly some inter ested specialists, to the lives of the yeasts in their natural and more artificial habitats, their use by human beings, and to give some idea of the wonderfully complex activities within the yeast cell, the characteristics of the metabolism and molecular biology of yeasts, and the applications of these characteristics to life in the present-dayworld ofhuman existence. The book proceeds from a brief chapter on what is and is not known of the origins and early history of the yeasts, through a description of their classification, relationships, habitats and general life style, their external morphology and internal structures and mechanisms within their cells, the regulatory mechanisms controlling processes such as signal transmis sion, mating, cell fusion, and many others.

Author : Valeria Mapelli
Publisher : Humana
Page : 0 pages
File Size : 48,7 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493952862

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Yeast Metabolic Engineering by Valeria Mapelli Pdf

Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.

Author : Johannes H. de Winde
Publisher : Springer Science & Business Media
Page : 367 pages
File Size : 45,5 Mb
Release : 2008-01-26
Category : Science
ISBN : 9783540370031

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Functional Genetics of Industrial Yeasts by Johannes H. de Winde Pdf

In recent years, new yeast species have proven their value and novel biotechnological applications have emerged. This book compiles the multi-faceted genetic repertoire of several yeasts relevant to modern biotechnology, and describes their utilization in research and application in the light of their genetic make-up and physiological characteristics. Moreover, the book presents a thorough overview of a wide array of methodologies from classical genetics to modern genomics technologies that have been and are being used in functional analysis of yeasts.

Author : Tulasi Satyanarayana,Gotthard Kunze
Publisher : Springer
Page : 486 pages
File Size : 46,9 Mb
Release : 2017-05-13
Category : Science
ISBN : 9789811026218

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Yeast Diversity in Human Welfare by Tulasi Satyanarayana,Gotthard Kunze Pdf

This book brings together and updates the latest information on the diversity of yeasts, their molecular features and their applications in the welfare of mankind. Yeasts are eukaryotic microfungi widely found in natural environments, including those with extreme conditions such as low temperatures, low oxygen levels and low water availability. To date, approximately 2,000 of the estimated 30,000 to 45,000 species of yeast on Earth, belonging to around 200 genera have been described. Although there are a few that are opportunistic human and animal pathogens, the vast majority of yeasts are beneficial, playing an important role in the food chain and in the carbon, nitrogen and sulphur cycles. In addition, yeasts such as Saccharomyces cerevisiae, Hansenula polymorpha and Pichia pastoris are used in expressing foreign genes to produce proteins of pharmaceutical interest. A landmark in biotechnology was reached in 1996 with the completion of sequencing of the entire S. cerevisiae genome, and it has now become a central player in the development of an entirely new approach to biological research and synthetic biology. The sequencing of genomes of several yeasts including Schizosaccharomyces pombe, Candida albicans and Cryptococcus neofromans has also recently been completed. candida albicans="" and="" p/pp

Author : Christine Guthrie,Gerald R. Fink
Publisher : Gulf Professional Publishing
Page : 980 pages
File Size : 51,6 Mb
Release : 2004
Category : Fungal molecular biology
ISBN : 012182778X

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Guide to Yeast Genetics and Molecular and Cell Biology by Christine Guthrie,Gerald R. Fink Pdf