Yeast Metabolic Engineering

Author : Valeria Mapelli,Maurizio Bettiga
Publisher : Springer Nature
Page : 300 pages
File Size : 49,5 Mb
Release : 2022-07-04
Category : Science
ISBN : 9781071623992

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Yeast Metabolic Engineering by Valeria Mapelli,Maurizio Bettiga Pdf

This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.

Author : Valeria Mapelli
Publisher : Humana
Page : 0 pages
File Size : 47,7 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493952862

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Yeast Metabolic Engineering by Valeria Mapelli Pdf

Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.

Author : Sang Yup Lee,Jens Nielsen,Gregory Stephanopoulos
Publisher : John Wiley & Sons
Page : 1075 pages
File Size : 52,9 Mb
Release : 2021-06-02
Category : Science
ISBN : 9783527823451

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Metabolic Engineering by Sang Yup Lee,Jens Nielsen,Gregory Stephanopoulos Pdf

Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering: Concepts and Applications delivers a one-stop resource for readers seeking a complete description of the concepts, models, and applications of metabolic engineering. This guide offers practical insights into the metabolic engineering of major cell lines, including E. Coli, Bacillus and Yarrowia Lipolytica, and organisms, including human, animal, and plant). The distinguished editors also offer readers resources on microbiome engineering and the use of metabolic engineering in bioremediation. Written in two parts, Metabolic Engineering begins with the essential models and strategies of the field, like Flux Balance Analysis, Quantitative Flux Analysis, and Proteome Constrained Models. It also provides an overview of topics like Pathway Design, Metabolomics, and Genome Editing of Bacteria and Eukarya. The second part contains insightful descriptions of the practical applications of metabolic engineering, including specific examples that shed light on the topics within. In addition to subjects like the metabolic engineering of animals, humans, and plants, you’ll learn more about: Metabolic engineering concepts and a historical perspective on their development The different modes of analysis, including flux balance analysis and quantitative flux analysis An illuminating and complete discussion of the thermodynamics of metabolic pathways The Genome architecture of E. coli, as well as genome editing of both bacteria and eukarya An in-depth treatment of the application of metabolic engineering techniques to organisms including corynebacterial, bacillus, and pseudomonas, and more Perfect for students of biotechnology, bioengineers, and biotechnologists, Metabolic Engineering: Concepts and Applications also has a place on the bookshelves of research institutes, biotechnological institutes and industry labs, and university libraries. It's comprehensive treatment of all relevant metabolic engineering concepts, models, and applications will be of use to practicing biotechnologists and bioengineers who wish to solidify their understanding of the field.

Author : Yongjun Wei,Boyang Ji,Xiao-Jun Ji,Tao Chen,Rodrigo Ledesma-Amaro
Publisher : Frontiers Media SA
Page : 90 pages
File Size : 49,8 Mb
Release : 2022-01-20
Category : Science
ISBN : 9782889741090

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Engineering Yeast to Produce Plant Natural Products by Yongjun Wei,Boyang Ji,Xiao-Jun Ji,Tao Chen,Rodrigo Ledesma-Amaro Pdf

Author : Farshad Darvishi Harzevili
Publisher : Springer Nature
Page : 377 pages
File Size : 44,5 Mb
Release : 2022-01-21
Category : Science
ISBN : 9783030896805

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Synthetic Biology of Yeasts by Farshad Darvishi Harzevili Pdf

This book covers recent advances and future trends in yeast synthetic biology, providing readers with an overview of computational and engineering tools, and giving insight on important applications. Yeasts are one of the most attractive microbial cell factories for the production of a wide range of valuable products, including pharmaceuticals, nutraceuticals, cosmetics, agrochemicals and biofuels. Synthetic biology tools have been developed to improve the metabolic engineering of yeasts in a faster and more reliable manner. Today, these tools are used to make synthetic pathways and rewiring metabolism even more efficient, producing products at high titer, rate, and yield. Split into two parts, the book opens with an introduction to rational metabolic pathway prediction and design using computational tools and their applications for yeast systems and synthetic biology. Then, it focuses on the construction and assembly of standardized biobricks for synthetic pathway engineering in yeasts, yeast cell engineering and whole cell yeast-based biosensors. The second part covers applications of synthetic biology to produce diverse and attractive products by some well-known yeasts. Given its interdisciplinary scope, the book offers a valuable asset for students, researchers and engineers working in biotechnology, applied microbiology, metabolic engineer ing and synthetic biology.

Author : Vipin Chandra Kalia,Adesh Kumar Saini
Publisher : Springer
Page : 412 pages
File Size : 50,5 Mb
Release : 2017-10-07
Category : Medical
ISBN : 9789811055119

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Metabolic Engineering for Bioactive Compounds by Vipin Chandra Kalia,Adesh Kumar Saini Pdf

This book comprehensively discusses the latest research in the area of metabolic engineering. Metabolic engineering solutions for bioactive compounds are now being derived by means of heterologous gene expression, in a wide range of organisms. The book provides an overview of the model systems being employed for metabolic manipulation to yield bioactive molecules, such as single-cell proteins, antibody generation, metabolites, proteases, chaperones, therapeutic proteins, nanomaterials, polymeric conjugates, dendrimers and nanoassemblies, Escherichia coli, Agrobacterium, Saccharomyces cerevisiae and cell lines, etc. In addition, it shares insights into the scope of these methods in the areas of prevention, diagnosis and treatment of diseases, e.g. immunotherapy for curing various diseases like cancer, allergies, autoimmune diseases, etc.

Author : Jennifer Rebecca Headman Van Vleet
Publisher : Unknown
Page : 210 pages
File Size : 45,9 Mb
Release : 2009
Category : Yeast fungi
ISBN : 110929610X

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Yeast Metabolic Engineering for Ethanol Production Guided by Gene Expression Analysis by Jennifer Rebecca Headman Van Vleet Pdf

Author : Christoph Wittmann,Sang Yup Lee
Publisher : Springer Science & Business Media
Page : 391 pages
File Size : 47,6 Mb
Release : 2012-06-15
Category : Medical
ISBN : 9789400745346

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Systems Metabolic Engineering by Christoph Wittmann,Sang Yup Lee Pdf

Systems Metabolic Engineering is changing the way microbial cell factories are designed and optimized for industrial production. Integrating systems biology and biotechnology with new concepts from synthetic biology enables the global analysis and engineering of microorganisms and bioprocesses at super efficiency and versatility otherwise not accessible. Without doubt, systems metabolic engineering is a major driver towards bio-based production of chemicals, materials and fuels from renewables and thus one of the core technologies of global green growth. In this book, Christoph Wittmann and Sang-Yup Lee have assembled the world leaders on systems metabolic engineering and cover the full story – from genomes and networks via discovery and design to industrial implementation practises. This book is a comprehensive resource for students and researchers from academia and industry interested in systems metabolic engineering. It provides us with the fundaments to targeted engineering of microbial cells for sustainable bio-production and stimulates those who are interested to enter this exiting research field.

Author : Farshad Darvishi,Mark Blenner,Rodrigo Ledesma-Amaro
Publisher : Frontiers Media SA
Page : 137 pages
File Size : 41,7 Mb
Release : 2021-10-22
Category : Science
ISBN : 9782889713240

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Synthetic Biology of Yeasts for the Production of Non-Native Chemicals, 2nd Edition by Farshad Darvishi,Mark Blenner,Rodrigo Ledesma-Amaro Pdf

Author : Rodrigo Ledesma-Amaro,Pablo Ivan Nikel,Francesca Ceroni
Publisher : Frontiers Media SA
Page : 142 pages
File Size : 48,8 Mb
Release : 2020-05-05
Category : Electronic
ISBN : 9782889637089

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Synthetic Biology-Guided Metabolic Engineering by Rodrigo Ledesma-Amaro,Pablo Ivan Nikel,Francesca Ceroni Pdf

This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Author : Andriy Sibirny
Publisher : Springer
Page : 568 pages
File Size : 41,6 Mb
Release : 2019-08-12
Category : Science
ISBN : 9783030211103

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Non-conventional Yeasts: from Basic Research to Application by Andriy Sibirny Pdf

This volume scopes several aspects of non-conventional yeast research prepared by the leading specialists in the field. An introduction on taxonomy and systematics enhances the reader’s knowledge on yeasts beyond established ones such as Saccharomyces cerevisiae. Biotechnological approaches that involve fungal utilization of unusual substrates, production of biofuels and useful chemicals as citric acid, glutathione or erythritol are discussed. Further, strategies for metabolic engineering based on knowledge on regulation of gene expression as well as sensing and signaling pathways are presented. The book targets researchers and advanced students working in Microbiology, Microbial Biotechnology and Biochemistry.

Author : Zhi-Hua Liu,Art Ragauskas
Publisher : Springer Nature
Page : 398 pages
File Size : 44,8 Mb
Release : 2021-04-30
Category : Technology & Engineering
ISBN : 9783030655846

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Emerging Technologies for Biorefineries, Biofuels, and Value-Added Commodities by Zhi-Hua Liu,Art Ragauskas Pdf

This book provides in-depth coverage on the latest concepts, systems, and technologies that are being utilized in biorefineries for the production of biofuels and value-added commodities. Written by internationally recognized experts, the book provides a comprehensive overview of pretreatment technology for biorefineries and biofuels, enzymatic hydrolysis and fermentation technology for biofuel production, and lignin valorization for developing new products from waste lignin. The book will be a valuable resource for researchers and professionals working in process engineering, product engineering, material science, and systems and synthetic biology in the fields of biorefining, biofuel, biomaterials, environmental waste utilization, and biotechnology.

Author : S Cortassa,M A Aon,A A Iglesias,D Lloyd
Publisher : World Scientific
Page : 264 pages
File Size : 43,8 Mb
Release : 2002-03-07
Category : Science
ISBN : 9789814489515

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An Introduction to Metabolic and Cellular Engineering by S Cortassa,M A Aon,A A Iglesias,D Lloyd Pdf

Metabolic and cellular engineering, as presented in this book, is a powerful alliance of two technologies: genetics–molecular biology and fermentation technology. Both are driven by continuous refinement of the basic understanding of metabolism, physiology and cellular biology (growth, division, differentiation), as well as the development of new mathematical modeling techniques. The authors' approach is original in that it integrates several disciplines into a coordinated scheme, i.e. microbial physiology and bioenergetics, thermodynamics and enzyme kinetics, biomathematics and biochemistry, genetics and molecular biology. Thus, it is called a transdisciplinary approach (TDA). The TDA provides the basis for the rational design of microorganisms or cells in a way that has rarely been utilized to its full extent. Contents:Matter and Energy BalancesCell Growth and Metabolite Production. Basic ConceptsMethods of Quantitation of Cellular “Processes Performance”Dynamic Aspects of Bioprocess BehaviorBioprocess Development with Plant CellsCellular Engineering Readership: Undergraduates, graduates and researchers in biomedical engineering, biochemistry and biotechnology. Keywords:Reviews:“The book provides very well selected examples that permit an easy comprehension of the achievements expected from the different techniques. I consider the book suitable for both experimentalists and theoreticians … it also provides an excellent selection of key references on the main techniques available in the field which are very useful for students and research workers.”Marta Cascante Serratosa Professor of Biochemistry and Molecular Biology University of Barcelona “A worked example towards the end of the book of the use of such a scheme was most welcome. The book is aimed at final year undergraduates and postgraduate researchers and is suitable for personal purchase.”Microbiology Today

Author : Thalita Peixoto Basso
Publisher : BoD – Books on Demand
Page : 136 pages
File Size : 55,5 Mb
Release : 2019-08-07
Category : Medical
ISBN : 9781789239270

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Yeasts in Biotechnology by Thalita Peixoto Basso Pdf

This book offers a broad understanding of several ways in which yeasts can be applied to the biotechnology industry. The seven chapters are grouped into three sections (apart from the "Introduction" section). The Animal Nutrition section comprises two chapters dealing with the utilization of yeast as a probiotic for animal nutrition. The Food Industry section addresses the utilization of yeast in food products. Finally, the Industrial Bioproducts section deals with the development of new yeast platforms as cell factories for biochemical production.

Author : Stephen Van Dien
Publisher : Springer
Page : 122 pages
File Size : 44,6 Mb
Release : 2016-07-16
Category : Science
ISBN : 9783319419664

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Metabolic Engineering for Bioprocess Commercialization by Stephen Van Dien Pdf

This volume reviews the current metabolic engineering tools and technologies from a practical point of view, and guides researchers as they overcome challenges at various stages of organism and bioprocess development. Microbes have been engineered to produce a variety of industrial products such as fuels, basic chemicals, fine chemicals, nutritional supplements, and pharmaceutical intermediates, and new tools such as gene synthesis, advanced cloning techniques, ‘omics’ analysis, and mathematical modeling have greatly accelerated the pace of innovation in the field. Written by leading experts in the field from both academia and industry, key topics include synthetic biology, pathway engineering, metabolic flux manipulation, adaptive evolution, and fermentation process scale-up. It is suitable for non-specialists, and is a valuable resource for anyone embarking on the exciting path to harnessing the metabolic potential of microorganisms.