The Crispr Cas Tool Kit For Genome Editing

Author : Aftab Ahmad,Sultan Habibullah Khan,Zulqurnain Khan
Publisher : Springer
Page : 333 pages
File Size : 42,6 Mb
Release : 2021-12-16
Category : Medical
ISBN : 9811663041

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The CRISPR/Cas Tool Kit for Genome Editing by Aftab Ahmad,Sultan Habibullah Khan,Zulqurnain Khan Pdf

This book discusses CRISPR/Cas- one of the most powerful tools available to scientists for genome editing. CRISPR/Cas is not only a genome editing tool, but researchers have also engineered it for gene regulation, genome imaging, base editing and epigenome regulations. This book describes the entire toolkit for CRISPR/Cas. The opening section gives an introduction to the technique and compares it with other genome editing tools. Further section gives a historical perspective of the tool, along with its detailed classification. The next chapters describe bioinformatic tools in CRISPR/Cas, and delivery methods for CRISPR/Cas. The book also discusses about the applications of CRISPR/Cas beyond genome editing and use of CRISPR for rewriting genetic codes. The book dedicates a section to the use of CRISPR in plants. The book culminates with a chapter on the current status, challenges and shortcomings of the CRISPR/Cas genome editing tool. The book would be highly interesting to students and researchers in molecular biology, biochemistry, biotechnology, food science, agriculture and plant sciences.

Author : Aftab Ahmad,Sultan Habibullah Khan,Zulqurnain Khan
Publisher : Springer Nature
Page : 342 pages
File Size : 41,7 Mb
Release : 2022-01-01
Category : Medical
ISBN : 9789811663055

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The CRISPR/Cas Tool Kit for Genome Editing by Aftab Ahmad,Sultan Habibullah Khan,Zulqurnain Khan Pdf

This book discusses CRISPR/Cas- one of the most powerful tools available to scientists for genome editing. CRISPR/Cas is not only a genome editing tool, but researchers have also engineered it for gene regulation, genome imaging, base editing and epigenome regulations. This book describes the entire toolkit for CRISPR/Cas. The opening section gives an introduction to the technique and compares it with other genome editing tools. Further section gives a historical perspective of the tool, along with its detailed classification. The next chapters describe bioinformatic tools in CRISPR/Cas, and delivery methods for CRISPR/Cas. The book also discusses about the applications of CRISPR/Cas beyond genome editing and use of CRISPR for rewriting genetic codes. The book dedicates a section to the use of CRISPR in plants. The book culminates with a chapter on the current status, challenges and shortcomings of the CRISPR/Cas genome editing tool. The book would be highly interesting to students and researchers in molecular biology, biochemistry, biotechnology, food science, agriculture and plant sciences.

Author : Rodolphe Barrangou,John van der Oost
Publisher : Springer Science & Business Media
Page : 300 pages
File Size : 47,6 Mb
Release : 2012-12-13
Category : Science
ISBN : 9783642346576

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CRISPR-Cas Systems by Rodolphe Barrangou,John van der Oost Pdf

CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.

Author : Anonim
Publisher : Academic Press
Page : 244 pages
File Size : 46,8 Mb
Release : 2021-03-28
Category : Science
ISBN : 9780323853224

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Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part A by Anonim Pdf

Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems, Part A presents a collation of chapters written by global, eminent scientists. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. Chapters in this release include An Introduction and applications of CRISPR-Cas Systems, History, evolution and classification of CRISPR-Cas associated systems, CRISPR based bacterial genome editing and removal of pathogens, CRISPR based genome editing and removal of human viruses, CRISPR based development of RNA editing and diagnostic platform, and much more. Additional sections cover Genome engineering in insects for control of vector borne diseases, Development of insect cell line using CRISPR technology, CRISPRing protozoan parasites to better understand the biology of diseases, CRISPR based genome editing of Caenorhabditis elegans, and a variety of other important topics. Offers a basic understanding and clear picture of genome editing CRISPR-Cas systems in different organisms Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for removal of virus, bacteria, fungi, protozoan, and many more Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, and many more

Author : Vijai Singh,Pawan K. Dhar
Publisher : Academic Press
Page : 386 pages
File Size : 48,8 Mb
Release : 2020-02-18
Category : Science
ISBN : 9780128181416

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Genome Engineering via CRISPR-Cas9 System by Vijai Singh,Pawan K. Dhar Pdf

Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields. Provides basic understanding and a clear picture on how to design, use and implement the CRISPR-Cas9 system in different organisms Explains how to create an animal model for disease research and screening purposes using CRISPR Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, neurology, cancer, industry, and many more

Author : Feng Zhang,Bing Yang,Qi-Jun Chen,Wendy Harwood
Publisher : Frontiers Media SA
Page : 80 pages
File Size : 54,8 Mb
Release : 2021-12-01
Category : Science
ISBN : 9782889717866

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New Genome Editing Tools and Resources: Enabling Gene Discovery and Functional Genomics by Feng Zhang,Bing Yang,Qi-Jun Chen,Wendy Harwood Pdf

Author : Luis María Vaschetto
Publisher : CRC Press
Page : 315 pages
File Size : 42,8 Mb
Release : 2022-01-31
Category : Mathematics
ISBN : 9781000540833

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CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases by Luis María Vaschetto Pdf

The CRISPR-Cas9 genome-editing system is creating a revolution in the science world. In the laboratory, CRISPR-Cas9 can efficiently be used to target specific genes, correct mutations and regulate gene expression of a wide array of cells and organisms, including human cells. CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases is a unique reading material for college students, academicians, and other health professionals interested in learning about the broad range of applications of CRISPR/Cas9 genetic scissors. Some topics included in this book are: the role of the CRISPR/Cas9 system in neuroscience, gene therapy, epigenome editing, genome mapping, cancer, virus infection control strategies, regulatory challenges and bioethical considerations.

Author : Anonim
Publisher : Academic Press
Page : 200 pages
File Size : 43,8 Mb
Release : 2021-04-29
Category : Science
ISBN : 9780323852982

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Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part B by Anonim Pdf

Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part B, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. It is a simple, rapid, precise, and cost-effective tool for genome editing and regulation of a wide range of organisms. It has gained scientific and public attention worldwide. This volume mainly covers insect cell line, protozoans, zebrafish, drosophila, CRISPRi, patents as well as technology transfer, and many more. This book is a key source of information available in a single volume. This book will be useful for not only beginners in genome engineering, but also students, researchers, scientists, policymakers, and stakeholders interested in harnessing the potential of reprogramming of the genomes in several areas. Offers basic understanding and a clear picture of genome editing CRISPR-Cas systems in different organisms Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for insect cell line, protozoans, zebrafish, drosophila, and many more Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, molecular biology and many more

Author : Rodolphe Barrangou,Erik J. Sontheimer,Luciano A. Marraffini
Publisher : John Wiley & Sons
Page : 308 pages
File Size : 41,5 Mb
Release : 2022-09-14
Category : Science
ISBN : 9781683670377

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CRISPR by Rodolphe Barrangou,Erik J. Sontheimer,Luciano A. Marraffini Pdf

“An excellent compendium of all things CRISPR from some of the leading minds in the field. With thorough coverage from every angle and beautifully detailed illustrations, this book is not to be missed!” Jennifer A. Doudna, Professor of Chemistry, Biochemistry & Molecular Biology, UC Berkeley; Founder, Innovative Genomics Institute; Nobel Laureate and coinventor of CRISPR technology “This journey through CRISPR biology and several of its breakthrough applications offers an exciting glimpse into one of the most beautiful and compelling fields in the life sciences.” David R. Liu, Director of the Merkin Institute at the Broad Institute of MIT and Harvard; Professor of Chemistry and Chemical Biology at Harvard University; coinventor of base editing and prime editing “A must read! The CRISPR topics, written by world-leading experts, span from the fascinating mechanistic underpinnings to the ingenious applications. One can read from start to finish or pick and choose themes. Either way, the book delivers utterly enjoyable learning!” Bonnie Bassler, Squibb Professor and Chair, Princeton University Department of Molecular Biology; Howard Hughes Medical Institute Investigator CRISPR-Cas systems have revolutionized the science of gene editing and their possible applications continue to expand, from basic research to potentially groundbreaking medical and commercial uses. Led by a distinguished team of editors, CRISPR: Biology and Applications explores the subject matter needed to delve into this fascinating area. Topics covered include: Classification and molecular mechanisms of CRISPR-Cas systems CRISPR-Cas evolution, regulation, expression, and function Uses for gene editing and modulation of gene expression CRISPR-based antimicrobials and phage resistance for medical and industrial purposes Written by internationally renowned authors, CRISPR: Biology and Applications serves as both an introductory guide for those new to the field and an authoritative reference for seasoned researchers whose work touches this evolving and headline- making science.

Author : Anshu Alok,Jitesh Kumar,Mahipal S. Shekhawat
Publisher : Unknown
Page : 0 pages
File Size : 45,5 Mb
Release : 2024
Category : Medical
ISBN : 1032162376

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Basics of Crispr/Cas Mediated Plant Genome Editing by Anshu Alok,Jitesh Kumar,Mahipal S. Shekhawat Pdf

"Basics of CRISPR mediated plant genome editing offers a comprehensive collection of chapters from various authors across the globe. This book contains the basic understanding and development of CRISPR/Cas9/12a/12b for genome editing in plants. Starting from bioinformatics approach to identify guide RNA, off-targets, design gRNA/multiplex gRNA, and an implementation the CRISPR-Cas9 system in different plant species. A clear description of classification of the existing CRISPR system, ortholog of Cas endonucleases, and its application in the development of future genome editing tools. A focus of CRISPR/Cas toolkit and their different delivery methods in different crop and fruit improvement. Another application and case studies of selected medicinal plants will be covered. The step wise information within the book offers an essential guide for graduate and master students, teachers, and scientists in academic and research institute as well as private companies working area of plant science"--

Author : Deepu Pandita,Anu Pandita
Publisher : CRC Press
Page : 463 pages
File Size : 48,8 Mb
Release : 2023-06-30
Category : Science
ISBN : 9781000771251

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CRISPR/Cas-Mediated Genome Editing in Plants by Deepu Pandita,Anu Pandita Pdf

With over 820 million people facing hunger in today’s world, the need of the hour is the design of plant varieties with high yield, improved traits, and resistance properties in order to mitigate the detrimental impacts of biotic and abiotic stress conditions on food crops. This volume highlights the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated nuclease proteins as a solution for sustainable agriculture. CRISPR/Cas-mediated genome editing (GE) provides a robust, simple, precise, efficient, economical, and revolutionary toolbox that leads to improved plant traits with enhanced yield, quality, and resistance against various stresses, including climate-related stress, insect pests and diseases, pollution-related stresses, etc. Key features of the book: Explains the differences between conventional breeding, genetically modified crops, and genome editing approaches using CRISPR/Cas Introduces the tools of genome editing, such as ZFNS (zinc finger nucleases), mega nucleases, and TLENS (transcription activator-like effector nucleases) Covers the history, origin, discovery, structure, and classification of CRISPR/Cas Examines the databases and computational approaches of CRISPR/Cas Highlights genome editing of important crop plants with CRISPR/Cas systems, including wheat, maize. tomato, Brassica crops, rice, fruits Explores potential applications of CRISPR/Cas systems for climate smart crops

Author : Anonim
Publisher : Academic Press
Page : 394 pages
File Size : 52,7 Mb
Release : 2021-06-12
Category : Science
ISBN : 9780323853248

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Reprogramming the Genome: CRISPR-Cas-based Human Disease Therapy by Anonim Pdf

Reprogramming the Genome: CRISPR-Cas-based Human Disease Therapy, presents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 is a key technology for targeted genome editing and regulation in a number of organisms including mammalian cells. It is a rapid, simple, and cost-effective solution. CRISPR-Cas system has recently gained much scientific and public attention. This volume covers CRISPR-Cas9 based mammalian genome editing, creating disease models, cancer therapy, neurological, heredity, blood disorders, defective gene correction, stem cells therapy, epigenetic modifications, patents, ethics, biosafety and regulatory issues challenges and opportunities. This book is a key source of information on mammalian genome editing available in a single volume. This book will be useful for beginners in mammalian genome editing and also students, researchers, scientists, policymakers, clinicians and stakeholders interested in genome editing in several areas. Offers basic understanding and a clear picture of mammalian genome editing through CRISPR-Cas systems Discusses how to create mammalian disease models, stem cell modification, epigenetic modifications, correction of defective gene in blood disorders, heredity, neurological disorders and many more Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, molecular biology, translational sciences, neurobiology, neurology, cancer, stem cells, and many more

Author : Kevin Davies
Publisher : Simon and Schuster
Page : 402 pages
File Size : 45,9 Mb
Release : 2020-10-06
Category : Science
ISBN : 9781643133942

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Editing Humanity by Kevin Davies Pdf

One of the world's leading experts on genetics unravels one of the most important breakthroughs in modern science and medicine. IIf our genes are, to a great extent, our destiny, then what would happen if mankind could engineer and alter the very essence of our DNA coding? Millions might be spared the devastating effects of hereditary disease or the challenges of disability, whether it was the pain of sickle-cell anemia to the ravages of Huntington’s disease. But this power to “play God” also raises major ethical questions and poses threats for potential misuse. For decades, these questions have lived exclusively in the realm of science fiction, but as Kevin Davies powerfully reveals in his new book, this is all about to change. Engrossing and page-turning, Editing Humanity takes readers inside the fascinating world of a new gene editing technology called CRISPR, a high-powered genetic toolkit that enables scientists to not only engineer but to edit the DNA of any organism down to the individual building blocks of the genetic code. Davies introduces readers to arguably the most profound scientific breakthrough of our time. He tracks the scientists on the front lines of its research to the patients whose powerful stories bring the narrative movingly to human scale. Though the birth of the “CRISPR babies” in China made international news, there is much more to the story of CRISPR than headlines seemingly ripped from science fiction. In Editing Humanity, Davies sheds light on the implications that this new technology can have on our everyday lives and in the lives of generations to come.

Author : Mason Hartley
Publisher : Bookademy
Page : 37 pages
File Size : 40,5 Mb
Release : 2024-06-27
Category : Science
ISBN : 8210379456XXX

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CRISPR by Mason Hartley Pdf

Explore the revolutionary world of CRISPR-Cas technology in this comprehensive book. From its origins and mechanisms to its applications in gene editing, biomedicine, agriculture, and beyond, discover the power and potential of this groundbreaking gene editing tool. Delve into the ethical considerations, challenges, and future prospects of CRISPR-Cas, and gain insights into how it is transforming scientific research and reshaping the way we approach genetic engineering. A must-read for anyone interested in the forefront of genetic technology.

Author : Shabir Hussain Wani,Goetz Hensel
Publisher : Springer Nature
Page : 338 pages
File Size : 46,9 Mb
Release : 2022-11-08
Category : Science
ISBN : 9783031080722

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Genome Editing by Shabir Hussain Wani,Goetz Hensel Pdf

Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.