The Immune Synapse

Author : Cosima T. Baldari,Michael L. Dustin
Publisher : Springer Nature
Page : 518 pages
File Size : 52,6 Mb
Release : 2023-04-27
Category : Science
ISBN : 9781071631355

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The Immune Synapse by Cosima T. Baldari,Michael L. Dustin Pdf

This new collection features the most up-to-date essential protocols that are currently being used to study the immune synapse. Beginning with methods for making biophysical measurements, the volume continues by covering the cell biology of synapses, methods for advanced substrate engineering, mechanobiology topics, new technologies to describe and manipulate synaptic components, as well as methods related to sites of action and immunotherapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, The Immune Synapse: Methods and Protocols, Second Edition serves as an ideal practical guide for researchers working in this dynamic field. Chapters 5, 11, 18, 27, 30, and 32 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : Luis Graca
Publisher : Springer Science & Business Media
Page : 192 pages
File Size : 53,6 Mb
Release : 2007-12-18
Category : Medical
ISBN : 9783764382964

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The Immune Synapse as a Novel Target for Therapy by Luis Graca Pdf

This volume gives an overview on the progress in immune synapse research, from basic science to clinical trials, and the major mechanisms involved. It discusses how interfering with T cell activation may lead to immune tolerance, immune modulation, and the recruitment of regulatory T cells; the role of monoclonal antibodies in tolerance induction; and mechanisms maintaining dominant tolerance.

Author : Takashi Saito,Facundo D. Batista
Publisher : Springer Science & Business Media
Page : 265 pages
File Size : 51,8 Mb
Release : 2009-12-03
Category : Medical
ISBN : 9783642038587

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Immunological Synapse by Takashi Saito,Facundo D. Batista Pdf

The proper physiological functioning of most eukaryotic cells requires their assembly into multi-cellular tissues that form organized organ systems. Cells of the immune system develop in bone marrow and lymphoid organs, but as the cells mature they leave these organs and circulate as single cells. Antigen receptors (TCRs) of T cells search for membrane MHC proteins that are bound to peptides derived from infectious pathogens or cellular transformations. The detection of such speci?c peptide–MHC antigens initiates T cell activation, adhesion, and immune-effectors functions. Studies of normal and transformed T cell lines and of T cells from transgenic mice led to comprehensive understanding of the mole- lar basis of antigen-receptor recognition and signaling. In spite of these remarkable genetic and biochemical advances, other key physiological mechanisms that par- cipate in sensing and decoding the immune context to induce the appropriate cellular immune responses remain unresolved. TCR recognition is tightly regulated to trigger sensitive but balanced T cell responses that result in the effective elimination of the pathogens while minimizing collateral damage to the host. The sensitivity of TCR recognition has to be properly tempered to prevent unintended activation by self-peptide–MHC complexes that cause autoimmune diseases. It is likely that once the TCR is engaged by a peptide– MHC and TCR signaling begins, additional regulatory mechanisms, involving other receptors, would increase the ?delity of the response.

Author : Anonim
Publisher : Elsevier
Page : 208 pages
File Size : 54,6 Mb
Release : 2023-01-16
Category : Science
ISBN : 9780323901550

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The Immunological Synapse Part A by Anonim Pdf

The Immunological Synapse, Part A, Volume 173 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. Sections cover Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: control by polarity regulators, Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse, P815-based redirected degranulation assay to study human NK cell effector functions, Cytotoxic and Chemotactic Dynamics of Natural Killer Cells Quantified by Live-cell Imaging, Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay, and more. Other chapters focus on the Study of the Effects of NK-Tumor Cell Interaction by Proteomic Analysis and Imaging, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, An in vitro model to monitor natural killer cell effector functions against primary breast cancer, and Standardized Protocol for the Evaluation of Chimeric Antigen Receptor (CAR)-modified Cell Immunological Synapse Quality using the Glass-supported Planar L. Covers various methods related to the study of the immunological synapse Includes detailed, point-by-point, methods as well as various important notes Provides the authority and expertise from an international board of leading scientists

Author : Pedro Roda-Navarro,Andrés Alcover,Vincenzo Di Bartolo
Publisher : Frontiers Media SA
Page : 122 pages
File Size : 49,8 Mb
Release : 2017-03-21
Category : Electronic book
ISBN : 9782889451333

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Molecular Dynamics at the Immunological Synapse by Pedro Roda-Navarro,Andrés Alcover,Vincenzo Di Bartolo Pdf

The immunological synapse (IS) is a specialised cell-cell adhesion that mediates antigen acquisition and regulates the activation of lymphocytes. Initial studies of the IS showed a structure composed of stable supra-molecular activation clusters (SMAC) organised during the interaction of helper T lymphocytes with B lymphocytes, working as antigen presenting cells. A central SMAC of coalesced T cell receptors (TCRs) and a peripheral SMAC for cell-cell adhesion were observed. IS with similar structure was later described during antigen acquisition by B cells and during the interaction of NK cells with target and healthy cells. More recent research developed with microscopy systems that improve the spatial and temporal resolution has showed the complex molecular dynamics at the IS that governs lymphocyte activation. Currently, the IS is seen as a three-dimensional structure where signalling networks for lymphocyte activation and endosomal and cytoskeleton machinery are polarised. A view has emerged in which dynamic microclusters of signalling complexes are composed of molecular components attached to the plasma membrane and other components conveyed on sub-synaptic vesicles transported to the membrane by cytoskeletal fibers and motor proteins. Much information is nonetheless missing about how the dynamics of the endosomal compartment, the cytoskeleton, and signalling complexes are reciprocally regulated to achieve the function of lymphocytes. Experimental evidence also suggests that the environment surrounding lymphocytes exposed to different antigenic challenge regulates IS assembly and functional output, making an even more complex scenario still far from being completely understood. Also, although some signalling molecular components for lymphocyte activation have been identified and thoroughly studied, the function of other molecules has not been yet uncovered or deeply characterised. This research topic aims to provide the reader with the latest information about the molecular dynamics governing lymphocyte activation. These molecular dynamics dictate cell decisions. Thus, we expect that understanding them will provide new avenues for cell manipulation in therapies to treat different immune-related pathologies.

Author : Takashi Saito,Facundo D. Batista
Publisher : Springer
Page : 255 pages
File Size : 52,7 Mb
Release : 2010-04-30
Category : Medical
ISBN : 3642038832

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Immunological Synapse by Takashi Saito,Facundo D. Batista Pdf

The proper physiological functioning of most eukaryotic cells requires their assembly into multi-cellular tissues that form organized organ systems. Cells of the immune system develop in bone marrow and lymphoid organs, but as the cells mature they leave these organs and circulate as single cells. Antigen receptors (TCRs) of T cells search for membrane MHC proteins that are bound to peptides derived from infectious pathogens or cellular transformations. The detection of such speci?c peptide–MHC antigens initiates T cell activation, adhesion, and immune-effectors functions. Studies of normal and transformed T cell lines and of T cells from transgenic mice led to comprehensive understanding of the mole- lar basis of antigen-receptor recognition and signaling. In spite of these remarkable genetic and biochemical advances, other key physiological mechanisms that par- cipate in sensing and decoding the immune context to induce the appropriate cellular immune responses remain unresolved. TCR recognition is tightly regulated to trigger sensitive but balanced T cell responses that result in the effective elimination of the pathogens while minimizing collateral damage to the host. The sensitivity of TCR recognition has to be properly tempered to prevent unintended activation by self-peptide–MHC complexes that cause autoimmune diseases. It is likely that once the TCR is engaged by a peptide– MHC and TCR signaling begins, additional regulatory mechanisms, involving other receptors, would increase the ?delity of the response.

Author : Anonim
Publisher : Elsevier
Page : 224 pages
File Size : 40,5 Mb
Release : 2023-07-27
Category : Science
ISBN : 9780443191640

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The Immunological Synapse - Part B by Anonim Pdf

The Immunological Synapse - Part B, Volume 178 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. This first volume covers various aspects on T cell and natural killer (NK) cell synapses, including imaging polarized granule release using TIRF microscopy, analysis of actin reorganization and centrosome polarization, redirected degranulation, live cell-imaging to quantify cytotoxic and chemotactic dynamics, quantification of interactions between APCs and T cells, assessment of membrane lipid state at the immunological synapse, proteomic analysis and imaging of NK-tumor cell interaction, evaluating natural killer cell effector functions against breast cancer cells derived from human tumor tissue, evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer, and monitoring potency of therapeutic CAR T cells. It also includes one chapter on quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, and one chapter on the murine antibody-dependent cellular phagocytosis assay. Covers various methods related to the study of the immunological synapse Includes detailed, point-by-point, methods as well as various important notes Provides the authority and expertise from an international board of leading scientists

Author : Anonim
Publisher : Unknown
Page : 0 pages
File Size : 52,5 Mb
Release : 2017
Category : Electronic
ISBN : OCLC:1368451523

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Molecular Dynamics at the Immunological Synapse by Anonim Pdf

The immunological synapse (IS) is a specialised cell-cell adhesion that mediates antigen acquisition and regulates the activation of lymphocytes. Initial studies of the IS showed a structure composed of stable supra-molecular activation clusters (SMAC) organised during the interaction of helper T lymphocytes with B lymphocytes, working as antigen presenting cells. A central SMAC of coalesced T cell receptors (TCRs) and a peripheral SMAC for cell-cell adhesion were observed. IS with similar structure was later described during antigen acquisition by B cells and during the interaction of NK cells with target and healthy cells. More recent research developed with microscopy systems that improve the spatial and temporal resolution has showed the complex molecular dynamics at the IS that governs lymphocyte activation. Currently, the IS is seen as a three-dimensional structure where signalling networks for lymphocyte activation and endosomal and cytoskeleton machinery are polarised. A view has emerged in which dynamic microclusters of signalling complexes are composed of molecular components attached to the plasma membrane and other components conveyed on sub-synaptic vesicles transported to the membrane by cytoskeletal fibers and motor proteins. Much information is nonetheless missing about how the dynamics of the endosomal compartment, the cytoskeleton, and signalling complexes are reciprocally regulated to achieve the function of lymphocytes. Experimental evidence also suggests that the environment surrounding lymphocytes exposed to different antigenic challenge regulates IS assembly and functional output, making an even more complex scenario still far from being completely understood. Also, although some signalling molecular components for lymphocyte activation have been identified and thoroughly studied, the function of other molecules has not been yet uncovered or deeply characterised. This research topic aims to provide the reader with the latest information about the molecular dynamics governing lymphocyte activation. These molecular dynamics dictate cell decisions. Thus, we expect that understanding them will provide new avenues for cell manipulation in therapies to treat different immune-related pathologies.

Author : Rashid Giniatullin,Kalpna Gupta,Theoharis Constantin Theoharides
Publisher : Frontiers Media SA
Page : 194 pages
File Size : 52,7 Mb
Release : 2020-01-27
Category : Electronic
ISBN : 9782889633470

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Mast Cells in Itch, Pain and Neuro-inflammation by Rashid Giniatullin,Kalpna Gupta,Theoharis Constantin Theoharides Pdf

Author : Anonim
Publisher : Unknown
Page : 0 pages
File Size : 44,5 Mb
Release : 2003
Category : Electronic
ISBN : OCLC:75277120

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The Immunological Synapse by Anonim Pdf

Author : Ernesto Oviedo-Orta,Brenda R. Kwak,William Howard Evans
Publisher : CRC Press
Page : 380 pages
File Size : 44,7 Mb
Release : 2013-03-22
Category : Medical
ISBN : 9781439862582

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Connexin Cell Communication Channels by Ernesto Oviedo-Orta,Brenda R. Kwak,William Howard Evans Pdf

Plasma membrane-associated channels known as gap junctions, along with their protein building blocks-connexins-have an important functional role in a range of immunological processes, including heart function, cell growth and specialization, and early development. Spanning basic science and potential clinical applications, Connexin Cell Communicati

Author : Ayse Basak Engin,Atilla Engin
Publisher : Springer Nature
Page : 415 pages
File Size : 47,7 Mb
Release : 2021-02-04
Category : Medical
ISBN : 9783030498443

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Protein Kinase-mediated Decisions Between Life and Death by Ayse Basak Engin,Atilla Engin Pdf

Protein phosphorylation via protein kinases is an inevitable process that alters physiological and pathological functions of the cells. Thus, protein kinases play key roles in the regulation of cell life or death decisions. Protein kinases are frequently a driving factor in a variety of human diseases including aging and cellular senescence, immune system and endothelial dysfunctions, cancers, insulin resistance, cholestasis and neurodegenerative diseases, as well as bacterial resistance in persistent infections. Recent developments in quantitative proteomics provide important opinions on kinase inhibitor selectivity and their modes of action in the biological context. Protein Kinase-mediated Decisions Between Life and Death aims to have the reader catch insights about up-to-date opinions on “Protein Kinases” related pathways that threaten human health and life. As “Protein Kinases” are related to many health problems, clinicians, basic science researchers and students need this information. Chapter “Signal Transduction in Immune Cells and Protein Kinases” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : Natasha S. Barteneva,Ivan A. Vorobjev
Publisher : Humana
Page : 0 pages
File Size : 42,6 Mb
Release : 2015-11-23
Category : Medical
ISBN : 1493933000

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Imaging Flow Cytometry by Natasha S. Barteneva,Ivan A. Vorobjev Pdf

This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

Author : I. KANNAN
Publisher : MJP Publisher
Page : 574 pages
File Size : 55,6 Mb
Release : 2019-06-11
Category : Medical
ISBN : 8210379456XXX

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IMMUNOLOGY by I. KANNAN Pdf

Immunology as a scientific discipline deals with the study of the immune system. This book on Immunology provides an in-depth coverage of the immune system and the various principles lying behind its effective functioning. The systematic organization of chapters with the inclusion of recent advances in the field of immunology make this a treatise. The topics are dealt in simple language with numerous illustrations to provide ease of learning. Important additional information relating to this field is provided as boxed items.

Author : Chaim Putterman,David Cowburn,Steven Almo
Publisher : Academic Press
Page : 192 pages
File Size : 44,8 Mb
Release : 2018-05-14
Category : Medical
ISBN : 9780128033708

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Structural Biology in Immunology by Chaim Putterman,David Cowburn,Steven Almo Pdf

Structural Biology in Immunology, Structure/Function of Novel Molecules of Immunologic Importance delivers important information on the structure and functional relationships in novel molecules of immunologic interest. Due to an increasingly sophisticated understanding of the immune system, the approach to the treatment of many immune-mediated diseases, including multiple sclerosis, systemic lupus erythematosus, rheumatoid arthritis, and inflammatory bowel disease has been dramatically altered. Furthermore, there is an increasing awareness of the critical role of the immune system in cancer biology. The improved central structure function relationships presented in this book will further enhance our ability to understand what defects in normal individuals can lead to disease. Describes novel/recently discovered immunomodulatory proteins, including antibodies and co-stimulatory or co-inhibitory molecules Emphasizes new biologic and small molecule drug design through the exploration of structure-function relationship Features a collaborative editorial effort, involving clinical immunologists and structural biologists Provides useful and practical insights on developing the necessary links between basic science and clinical therapy in immunology Gives interested parties a bridge to learn about computer modeling and structure based design principles