Total Chemical Synthesis Of Proteins

Author : Ashraf Brik,Philip Dawson,Lei Liu
Publisher : John Wiley & Sons
Page : 624 pages
File Size : 53,5 Mb
Release : 2021-02-23
Category : Science
ISBN : 9783527823574

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Total Chemical Synthesis of Proteins by Ashraf Brik,Philip Dawson,Lei Liu Pdf

How to synthesize native and modified proteins in the test tube With contributions from a panel of experts representing a range of disciplines, Total Chemical Synthesis of Proteins presents a carefully curated collection of synthetic approaches and strategies for the total synthesis of native and modified proteins. Comprehensive in scope, this important reference explores the three main chemoselective ligation methods for assembling unprotected peptide segments, including native chemical ligation (NCL). It includes information on synthetic strategies for the complex polypeptides that constitute glycoproteins, sulfoproteins, and membrane proteins, as well as their characterization. In addition, important areas of application for total protein synthesis are detailed, such as protein crystallography, protein engineering, and biomedical research. The authors also discuss the synthetic challenges that remain to be addressed. This unmatched resource: Contains valuable insights from the pioneers in the field of chemical protein synthesis Presents proven synthetic approaches for a range of protein families Explores key applications of precisely controlled protein synthesis, including novel diagnostics and therapeutics Written for organic chemists, biochemists, biotechnologists, and molecular biologists, Total Chemical Synthesis of Proteins provides key knowledge for everyone venturing into the burgeoning field of protein design and synthetic biology.

Author : Ashraf Brik,Philip Dawson,Lei Liu
Publisher : John Wiley & Sons
Page : 624 pages
File Size : 43,5 Mb
Release : 2021-03-16
Category : Science
ISBN : 9783527823581

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Total Chemical Synthesis of Proteins by Ashraf Brik,Philip Dawson,Lei Liu Pdf

How to synthesize native and modified proteins in the test tube With contributions from a panel of experts representing a range of disciplines, Total Chemical Synthesis of Proteins presents a carefully curated collection of synthetic approaches and strategies for the total synthesis of native and modified proteins. Comprehensive in scope, this important reference explores the three main chemoselective ligation methods for assembling unprotected peptide segments, including native chemical ligation (NCL). It includes information on synthetic strategies for the complex polypeptides that constitute glycoproteins, sulfoproteins, and membrane proteins, as well as their characterization. In addition, important areas of application for total protein synthesis are detailed, such as protein crystallography, protein engineering, and biomedical research. The authors also discuss the synthetic challenges that remain to be addressed. This unmatched resource: Contains valuable insights from the pioneers in the field of chemical protein synthesis Presents proven synthetic approaches for a range of protein families Explores key applications of precisely controlled protein synthesis, including novel diagnostics and therapeutics Written for organic chemists, biochemists, biotechnologists, and molecular biologists, Total Chemical Synthesis of Proteins provides key knowledge for everyone venturing into the burgeoning field of protein design and synthetic biology.

Author : Xuechen Li
Publisher : Springer Nature
Page : 258 pages
File Size : 50,6 Mb
Release : 2022-06-27
Category : Science
ISBN : 9781071624890

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Chemical Protein Synthesis by Xuechen Li Pdf

This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chemical Protein Synthesis aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Author : Lei Liu
Publisher : Springer
Page : 235 pages
File Size : 46,7 Mb
Release : 2015-05-15
Category : Science
ISBN : 9783319191898

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Protein Ligation and Total Synthesis II by Lei Liu Pdf

Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field.

Author : Paul Lloyd-Williams,Fernando Albericio,Ernest Giralt
Publisher : CRC Press
Page : 306 pages
File Size : 55,5 Mb
Release : 1997-04-23
Category : Medical
ISBN : 0849391423

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Chemical Approaches to the Synthesis of Peptides and Proteins by Paul Lloyd-Williams,Fernando Albericio,Ernest Giralt Pdf

Organic chemists working on the synthesis of natural products have long found a special challenge in the preparation of peptides and proteins. However, more reliable, more efficient synthetic preparation methods have been developed in recent years. This reference evaluates the most important synthesis methods available today, and also considers methods that show promise for future applications. This text describes the state of the art in efficient synthetic methods for the synthesis of both natural and artificial large peptide and protein molecules. Subjects include an introduction to basic topics, linear solid-phase synthesis of peptides, peptide synthesis in solution, convergent solid-phase synthesis, methods for the synthesis of branched peptides, formation of disulfide bridges, and more. The book emphasizes strategies and tactics that must be considered for the successful synthesis of peptides.

Author : Luca D. D'Andrea,Alessandra Romanelli
Publisher : John Wiley & Sons
Page : 576 pages
File Size : 54,9 Mb
Release : 2017-03-16
Category : Science
ISBN : 9781119044093

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Chemical Ligation by Luca D. D'Andrea,Alessandra Romanelli Pdf

Presenting a wide array of information on chemical ligation – one of the more powerful tools for protein and peptide synthesis – this book helps readers understand key methodologies and applications that protein therapeutic synthesis, drug discovery, and molecular imaging. • Moves from fundamental to applied aspects, so that novice readers can follow the entire book and apply these reactions in the lab • Presents a wide array of information on chemical ligation reactions, otherwise scattered across the literature, into one source • Features comprehensive and multidisciplinary coverage that goes from basics to advanced topics • Helps researchers choose the right chemical ligation technique for their needs

Author : Paul Lloyd-Williams,Fernando Albericio,Ernest Giralt
Publisher : CRC Press
Page : 306 pages
File Size : 47,7 Mb
Release : 2020-08-18
Category : Cooking
ISBN : 9781000151688

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Chemical Approaches to the Synthesis of Peptides and Proteins by Paul Lloyd-Williams,Fernando Albericio,Ernest Giralt Pdf

Organic chemists working on the synthesis of natural products have long found a special challenge in the preparation of peptides and proteins. However, more reliable, more efficient synthetic preparation methods have been developed in recent years. This reference evaluates the most important synthesis methods available today, and also considers methods that show promise for future applications. This text describes the state of the art in efficient synthetic methods for the synthesis of both natural and artificial large peptide and protein molecules. Subjects include an introduction to basic topics, linear solid-phase synthesis of peptides, peptide synthesis in solution, convergent solid-phase synthesis, methods for the synthesis of branched peptides, formation of disulfide bridges, and more. The book emphasizes strategies and tactics that must be considered for the successful synthesis of peptides.

Author : Lei Liu
Publisher : Springer
Page : 270 pages
File Size : 45,7 Mb
Release : 2015-05-15
Category : Science
ISBN : 9783319191867

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Protein Ligation and Total Synthesis I by Lei Liu Pdf

Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 50,6 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Hans Neurath
Publisher : Elsevier
Page : 603 pages
File Size : 44,9 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323162104

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The Proteins Pt 3 by Hans Neurath Pdf

The Proteins, Third Edition, Volume II is a three-chapter text that highlights the application of methods of organic chemistry to the study of protein structure. Chapter 1 reviews the chemical modification of proteins by group- and site-specific reagents. This chapter also discusses the methods for the chemical modification of proteins and their application to the study of the structure, conformation, and biologic activity of certain proteins. Chapter 2 describes the synthesis of peptides by solution methods and the procedures employed for the synthesis of biologically active peptide hormones. This chapter also deals with the many difficulties inherent in the application of the existing synthetic methods and emphasizes the stringent standards that must be maintained for the successful chemical synthesis of naturally occurring polypeptides. Chapter 3 presents the solid-phase methods for the synthesis of peptides on solid supports. Organic chemists and researchers, teachers and undergraduate students will find this book invaluable.

Author : Carmichael J.A. Wallace
Publisher : CRC Press
Page : 300 pages
File Size : 46,5 Mb
Release : 1999-09-29
Category : Medical
ISBN : 0849347270

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Protein Engineering by Semisynthesis by Carmichael J.A. Wallace Pdf

As genomics gives way to proteomics as the focus of scientific imagination in the biological sciences, more emphasis will be placed on the technology and interpretation of protein engineering experiments. Protein engineers will become increasingly sophisticated in the questions that they pose and demanding of the tools available to change protein structure. The optimal way to introduce non-coded amino acids for mechanistic studies, or site-specific reporter atoms for spectroscopic structural biology, is by protein semisynthesis. In Protein Engineering by Semisynthesis, the leading practitioners of the method cover their individual protein of expertise forming a comprehensive illustration of the various methods developed. By covering the most recent philosophical and methodological approaches and developments of semisynthesis and peptide synthesis to date, this book provides further understanding of the principles of protein structure-function relationships gained from semisynthetic analog in addition to providing a comprehensive and comprehensible laboratory guide. This book focuses on recent developments which synergistically combine chemical and molecular biological techniques that have made semisynthetic manipulations much easier to undertake. Features

Author : Miquel Vila-Perelló
Publisher : Humana
Page : 0 pages
File Size : 43,5 Mb
Release : 2020-03-07
Category : Science
ISBN : 1071604333

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Expressed Protein Ligation by Miquel Vila-Perelló Pdf

This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.

Author : Biman Bagchi
Publisher : Cambridge University Press
Page : 383 pages
File Size : 46,6 Mb
Release : 2013-11-14
Category : Medical
ISBN : 9781107037298

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Water in Biological and Chemical Processes by Biman Bagchi Pdf

A unified overview of the dynamical properties of water and its unique and diverse role in biological and chemical processes.

Author : Ron Milo,Rob Phillips
Publisher : Garland Science
Page : 400 pages
File Size : 53,7 Mb
Release : 2015-12-07
Category : Science
ISBN : 9781317230694

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Cell Biology by the Numbers by Ron Milo,Rob Phillips Pdf

A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid

Author : Ashraf Brik,Philip Dawson,Lei Liu
Publisher : John Wiley & Sons
Page : 626 pages
File Size : 47,9 Mb
Release : 2021-06-08
Category : Science
ISBN : 9783527346608

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Total Chemical Synthesis of Proteins by Ashraf Brik,Philip Dawson,Lei Liu Pdf

How to synthesize native and modified proteins in the test tube With contributions from a panel of experts representing a range of disciplines, Total Chemical Synthesis of Proteins presents a carefully curated collection of synthetic approaches and strategies for the total synthesis of native and modified proteins. Comprehensive in scope, this important reference explores the three main chemoselective ligation methods for assembling unprotected peptide segments, including native chemical ligation (NCL). It includes information on synthetic strategies for the complex polypeptides that constitute glycoproteins, sulfoproteins, and membrane proteins, as well as their characterization. In addition, important areas of application for total protein synthesis are detailed, such as protein crystallography, protein engineering, and biomedical research. The authors also discuss the synthetic challenges that remain to be addressed. This unmatched resource: Contains valuable insights from the pioneers in the field of chemical protein synthesis Presents proven synthetic approaches for a range of protein families Explores key applications of precisely controlled protein synthesis, including novel diagnostics and therapeutics Written for organic chemists, biochemists, biotechnologists, and molecular biologists, Total Chemical Synthesis of Proteins provides key knowledge for everyone venturing into the burgeoning field of protein design and synthetic biology.