Transforming Growth Factor Beta Protocols

Author : Philip H. Howe
Publisher : Springer Science & Business Media
Page : 176 pages
File Size : 41,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590537

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Transforming Growth Factor-Beta Protocols by Philip H. Howe Pdf

Philip H. Howe and a group of well-versed experimentalists present the first major volume a collection of indispensable classic and cutting-edge TGFß assays. Described in great detail to ensure robust and successful results, these readily reproducible techniques range from the growth inhibition assay for TGFß to methods for monitoring its interactions with the mediating proteins. Extensive notes discuss potential pitfalls and provide tips on how to avoid failures, and throughout, emphasis is given to detailing those technical steps critical for experimental success that are often omitted in the primary literature. Concise and highly practical, Transforming Growth Factor-Beta Protocols provides today's molecular and cell biologists-both expert and novice-with time-tested methods for the identification and analysis of the signal transduction pathways by which TGFß induces and modulates physiological behavior.

Author : Zhike Zi,Xuedong Liu
Publisher : Humana
Page : 239 pages
File Size : 49,8 Mb
Release : 2022-03-29
Category : Sports & Recreation
ISBN : 1071622765

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TGF-Beta Signaling by Zhike Zi,Xuedong Liu Pdf

This detailed volume is devoted to the recent development of quantitative experiments and computational methods driving new transforming growth factor beta (TGF-β) and other cell signaling knowledge. Many chapters cover quantitative assays for TGF-β signaling studies, with others exploring the increasing role of both modeling and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, TGF-Beta Signaling: Methods and Protocols serves as a vital resource for researchers seeking to move the TGF-β field into the quantitative realm.

Author : Xin-Hua Feng,Pinglong Xu,Xia Lin (Professor of Surgery)
Publisher : Unknown
Page : 394 pages
File Size : 55,9 Mb
Release : 2016
Category : Cellular signal transduction
ISBN : 1493929666

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TGF-[beta] Signaling by Xin-Hua Feng,Pinglong Xu,Xia Lin (Professor of Surgery) Pdf

Author : Xin-Hua Feng,Pinglong Xu,Xia Lin
Publisher : Unknown
Page : 0 pages
File Size : 50,6 Mb
Release : 2016
Category : Cytology
ISBN : 1493929674

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TGF- Signaling by Xin-Hua Feng,Pinglong Xu,Xia Lin Pdf

This volume details up-to-date information and attempts to take the reader into the exciting realm of TGF- from the basic principles to the practical applications. Chapters provide basic introduction of TGF- signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF- signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Author : John Varga,David A. Brenner,Sem H. Phan
Publisher : Springer Science & Business Media
Page : 393 pages
File Size : 45,7 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592599400

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Fibrosis Research by John Varga,David A. Brenner,Sem H. Phan Pdf

Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it. In their discussion of research methodologies utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Author : Sonia B. Jakowlew
Publisher : Springer Science & Business Media
Page : 726 pages
File Size : 48,6 Mb
Release : 2010-01-01
Category : Medical
ISBN : 9781597452922

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Transforming Growth Factor-Beta in Cancer Therapy, Volume I by Sonia B. Jakowlew Pdf

Transforming Growth Factor- ß in Cancer Therapy, Vols. 1 and 2, provides a compendium of findings about the role of transforming growth factor- ß (TGF- ß) in cancer treatment and therapy. The first volume, Basic and Clinical Biology, is divided into three parts. This volume’s companion, Cancer Treatment in Therapy, examines transforming growth factor- ß in other developing and advanced cancers and methods of treatment and therapy.

Author : John P. Neoptolemos,Raul A. Urrutia,James Abbruzzese,Markus W. Büchler
Publisher : Springer Science & Business Media
Page : 1419 pages
File Size : 41,5 Mb
Release : 2010-01-22
Category : Medical
ISBN : 9780387774978

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Pancreatic Cancer by John P. Neoptolemos,Raul A. Urrutia,James Abbruzzese,Markus W. Büchler Pdf

This handbook details advances in pancreatic cancer research that have a profound and lasting impact on the field. It features the work of authors who are the very best in their respective fields and will have widespread appeal among clinicians, pathologists and basic scientists who are now struggling to understand this complex and rapidly expanding field.

Author : Anonim
Publisher : ScholarlyEditions
Page : 182 pages
File Size : 46,8 Mb
Release : 2013-06-21
Category : Science
ISBN : 9781481693066

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Transforming Growth Factors—Advances in Research and Application: 2013 Edition by Anonim Pdf

Transforming Growth Factors—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Transforming Growth Factor alpha. The editors have built Transforming Growth Factors—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Transforming Growth Factor alpha in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Transforming Growth Factors—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Author : B. Kyewski,Elisabeth Suri-Payer
Publisher : Springer Science & Business Media
Page : 331 pages
File Size : 52,5 Mb
Release : 2006-01-09
Category : Medical
ISBN : 9783540277026

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CD4+CD25+ Regulatory T Cells: Origin, Function and Therapeutic Potential by B. Kyewski,Elisabeth Suri-Payer Pdf

The vertebrate immune system defends the organism against invading pathogens while at the same time being self-tolerant to the body’s own constituents thus preserving its integrity. Multiple mechanisms work in concert to ensure self-tolerance. Apart from purging the T cell repertoire from auto-reactive T cells via negative selection in the thymus dominant tolerance exerted by regulatory T cells plays a major role in tolerance imposition and maintenance. Among the various regulatory/suppressive cells hitherto described, CD4+CD25+ regulatory T cells (Treg) and interleukin-10 producing T regulatory 1 (Tr1) cells have been studied in most detail and are the subject of most articles in this issue. Treg, also called "natural" regulatory T cells, will be traced from their intra-thymic origin to the site of their action in peripheral lymphoid organs and tissues. The repertoire of Treg is clearly biased towards recognition of self-antigens, thereby potentially preventing autoimmune diseases such as gastritis and oophoritis. Regulatory T cells, however also control infections, allergies and tolerance to transplanted tissues and this requires their induction in the periphery under conditions which are not yet fully understood. The concept of dominant tolerance, by far not novel, will offer new insights and hopefully tools for the successful treatment of autoimmune diseases, improved cancer immunotherapy and transplant survival. The fulfillment of these high expectations will, however, require their unambiguous identification and a better understanding of their mode of action.

Author : Gustav Steinhoff
Publisher : Springer
Page : 396 pages
File Size : 51,5 Mb
Release : 2016-06-14
Category : Science
ISBN : 9783319283869

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Regenerative Medicine - from Protocol to Patient by Gustav Steinhoff Pdf

​Regenerative medicine is the main field of groundbreaking medical development and therapy using knowledge from developmental and stem cell biology as well as advanced molecular and cellular techniques. This collection of volumes on Regenerative Medicine: From Protocol to Patient, aims to explain the scientific knowledge and emerging technology as well as the clinical application in different organ systems and diseases. International leading experts from all over the world describe the latest scientific and clinical knowledge of the field of regenerative medicine. The process of translating science of laboratory protocols into therapies is explained in sections on regulatory, ethical and industrial issues. This collection is organized into five volumes: (1) Biology of Tissue Regeneration, (2) Stem Cell Science and Technology, (3) Tissue Engineering, Biomaterials and Nanotechnology, (4) Regenerative Therapies I, and (5) Regenerative Therapies II. The textbook gives the student, the researcher, the health care professional, the physician and the patient a complete survey on the current scientific basis, therapeutical protocols, clinical translation and practiced therapies in regenerative medicine. Volume 5 contains clinical science and translation surveys on the circulatory system, visceral, musculoskeletal and skin. The state-of-the-art descriptions involve concepts for clinical diagnosis, stem cell and gene therapy, biomaterials for tissue replacement and pharmacological/biomolecule treatment strategies.

Author : Keith R. Mitchelson,Jing Cheng
Publisher : Springer Science & Business Media
Page : 481 pages
File Size : 42,7 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590551

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Capillary Electrophoresis of Nucleic Acids by Keith R. Mitchelson,Jing Cheng Pdf

The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

Author : John R. Chapman
Publisher : Springer Science & Business Media
Page : 539 pages
File Size : 55,6 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590452

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Mass Spectrometry of Proteins and Peptides by John R. Chapman Pdf

Little more than three years down the line and I am already writing the Preface to a second volume to follow Protein and Peptide Analysis by Mass . What has happened in between these times to make this second venture worthwhile? New types of mass spectrometric instrumentation have appeared so that new techniques have become possible and existing techniques have become much more feasible. More particularly, however, the newer ionization te- niques, introduced for the analysis of high molecular weight materials, have now been thoroughly used and studied. As a result, there has been an en- mous improvement in the associated sample handling technology so that these methods are now routinely applied to much smaller sample amounts as well as to more intractable samples. Again, this particular community of mass spectrometry users has both increased in number and diversified. And, riding this wave of acceptance, leaders in the field have set their sights on more complex problems: molecular interaction, ion structures, quantitation, and kinetics are just a few of the newer areas reported in Mass Spectrometry of Proteins and Peptides. As with the first volume, one purpose of this collection, Mass Spectr- etry of Proteins and Peptides, is to show the reader what can be done by the application of mass spectrometry, and perhaps even to encourage the reader to venture down new paths.

Author : Kenneth P. Murphy
Publisher : Springer Science & Business Media
Page : 258 pages
File Size : 48,8 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592591930

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Protein Structure, Stability, and Folding by Kenneth P. Murphy Pdf

In Protein Structure, Stability, and Folding, Kenneth P. Murphy and a panel of internationally recognized investigators describe some of the newest experimental and theoretical methods for investigating these critical events and processes. Among the techniques discussed are the many methods for calculating many of protein stability and dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches presented include the use of co-solvents, novel applications of hydrogen exchange techniques, temperature-jump methods for looking at folding events, and new strategies for mutagenesis experiments. Unique in its powerful combination of theory and practice, Protein Structure, Stability, and Folding offers protein and biophysical chemists the means to gain a more comprehensive understanding of some of this complex area by detailing many of the major techniques in use today.

Author : Anonim
Publisher : Unknown
Page : 1568 pages
File Size : 55,5 Mb
Release : 2003
Category : Medicine
ISBN : MINN:31951P00880604F

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Index Medicus by Anonim Pdf

Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.

Author : B. Anne Croy,Aureo T. Yamada,Francesco J. DeMayo,S. Lee Adamson
Publisher : Academic Press
Page : 832 pages
File Size : 51,8 Mb
Release : 2013-12-09
Category : Science
ISBN : 9780123947949

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The Guide to Investigation of Mouse Pregnancy by B. Anne Croy,Aureo T. Yamada,Francesco J. DeMayo,S. Lee Adamson Pdf

The Guide to Investigation of Mouse Pregnancy is the first publication to cover the mouse placenta or the angiogenic tree the mother develops to support the placenta. This much-needed resource covers monitoring of the cardiovascular system, gestational programming of chronic adult disease, epigenetic regulation, gene imprinting, and stem cells. Offering detailed and integrated information on how drugs, biologics, stress, and manipulations impact pregnancy in the mouse model, this reference highlights techniques used to analyze mouse pregnancy. Joining the ranks of much referenced mouse resources, The Guide to Investigation of Mouse Pregnancy is the only manual providing needed content on pregnancy in animal models for translational medicine and research. Provides instruction on how to collect pre-clinical data on pregnancy in mouse models for eventual use in human applications Describes the angiogenic tree the mother’s uterus develops to support pregnancy and the monitoring of pregnancy-induced cardiovascular changes Educates readers on placental cell lineages, decidual development including immune cells, epigenetic regulation, gene imprinting, stem cells, birth and lactation Discusses how stress, environmental toxicants and other manipulations impact upon placental function and pregnancy success