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Veterinary PCR Diagnostics by Chengming Wang,Bernhard Kaltenboeck,Mark D. Freeman Pdf
"PCR (Polymerase Chain Reaction) technology has become an indispensable component of routine veterinary diagnostics. However, a number of pitfalls and limiting factors affect its sensitivity and specificity of detection. It is imperative that veterinary "
Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications by Erika Pestana,Sandor Belak,Adama Diallo,John R. Crowther,Gerrit J. Viljoen Pdf
This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. The book is an adjunct to the Molecular Diagnostic PCR Handbook published in 2005.
Author : E. van Pelt-Verkuil,W.B. van Leeuwen,R. te Witt Publisher : Springer Page : 237 pages File Size : 50,5 Mb Release : 2017-07-19 Category : Medical ISBN : 9789811045110
Molecular Diagnostics by E. van Pelt-Verkuil,W.B. van Leeuwen,R. te Witt Pdf
This two volume book set provides a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are currently used in a wide variety of disciplines. The volumes cover: Clinical microbiology and virology Clinical chemistry Pathology Veterinary medicine Plant Pathology Food safety The two volumes are written by experts in their respective fields, who have, together with the editors, combined years of experience in the development, application and quality control of molecular diagnostic methods. The first book is devoted to the theory and backgrounds of molecular techniques, amplification technology, next generation sequencing and bioinformatics for molecular laboratory diagnostics. As a fundament of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is extensively discussed. The second book highlights the applications of these methods in the various diagnostic laboratories. These two full-colour well-illustrated volumes are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic tests and for those who wish to expand their knowledge on the current molecular biological revolution. The extensive information in both books highlights the current trend of the integration of multiple (clinical) disciplines into one universal molecular laboratory.
Author : Michael E. Peterson,Patricia A. Talcott Publisher : Elsevier Health Sciences Page : 928 pages File Size : 42,8 Mb Release : 2013-08-07 Category : Medical ISBN : 9780323241984
Small Animal Toxicology - E-Book by Michael E. Peterson,Patricia A. Talcott Pdf
Diagnose and determine treatment for toxic exposures in small animals with this quick reference! Small Animal Toxicology, 3rd Edition covers hundreds of potentially toxic substances, providing the information you need to manage emergency treatment and prevent poisonings in companion animals. To help you identify an unknown poison, this guide provides a list of potential toxins based on clinical signs or symptoms. It also includes a NEW color insert with 85 full-color photographs of toxic plants and of lesions associated with various poisonings. Written by respected veterinarian Michael E. Peterson and board-certified veterinary toxicologist Patricia A. Talcott, along with a team of expert contributors, this edition covers a wide variety of topics including toxicodynamics, toxicokinetics, effective history taking, recognizing clinical signs of toxic exposures, managing emergencies, and supportive care of the poisoned patient. Comprehensive coverage of toxins/poisons includes the full range of substances from acetaminophen to zinc, including home products, prescription medicines, recreational drugs, and more. Guidelines to evaluation, diagnosis and treatment include examinations of the source, toxic dose, toxicokinetics, clinical signs, minimum database, confirming tests, treatment progress and differential diagnosis for each specific toxicant. Coverage of common poisonous substances includes grapes and raisins, nicotine, mercury, mushrooms, Christmas-time plants, and snake and spider venoms. Toxicological Concepts section provides information on toxicologic principles such as history taking, providing supportive care, and managing emergency treatment. General Exposures section addresses nontraditional toxicology such as indoor environmental air, pesticides, pharmaceuticals, and toxicities in pregnant and lactating animals. Miscellaneous Toxicant Groups section covers commonly encountered specific toxicants, the proper use of diagnostic laboratories, use of human poison control centers, and antidotes for specific toxins. More than 50 international contributors provide up-to-date, authoritative advice on treating poisonings and intoxications. 8 NEW chapters cover topics including legal considerations in toxicology cases, responding to mass exposures, and poisonings in birds, small mammals, and geriatric patients. NEW color insert shows 85 of the most commonly encountered toxic substances for at-a-glance identification. UPDATED Signs and Symptoms index makes it easier to find information on a toxic agent by presenting signs rather than requiring the formulation of a diagnosis. UPDATED information on agents most likely to cause a toxic reaction includes natural flea products and an expanded section on human medications. NEW quick-access format with bold headings and convenient tables and boxes allows quick retrieval of information in emergency situations.
Author : Gerrit J. Viljoen,Louis H. Nel,John R. Crowther Publisher : Springer Science & Business Media Page : 342 pages File Size : 46,9 Mb Release : 2005-07-19 Category : Medical ISBN : 1402034032
Molecular Diagnostic PCR Handbook by Gerrit J. Viljoen,Louis H. Nel,John R. Crowther Pdf
PREFACE The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR laboratory; Good Laboratory Practice and standardised of PCR protocols.
Veterinary Cytology by Leslie C. Sharkey,M. Judith Radin,Davis M. Seelig Pdf
This book offers a state-of-the-art, evidence-based reference to all aspects of veterinary cytology. Truly multidisciplinary in its approach, chapters are written by experts in fields ranging from clinical pathology to internal medicine, surgery, ophthalmology, and dermatology, drawing the various specialties together to create a comprehensive picture of cytology's role in diagnosis and treatment of animal disease. Firmly grounded in the primary literature, the book focuses on companion animals, with special chapters for species with fewer publications. Chapters are logically organized by body system, with additional chapters on tumors of particular import and diagnostic decision making. The first two sections of Veterinary Cytology focus on cytology techniques, quality control, and special laboratory techniques. Subsequent sections are organ/tissue-based and reflect what is known about the canine, feline, and equine species. This is followed by chapters on non-traditional species, including exotic companion mammals, rabbits, cattle, camelids, non-human primates, reptiles and birds, amphibians, fish, invertebrates, and sheep and goats. The last section highlights some unique features of the applications of cytology in industry settings. Provides a gold-standard reference to data-driven information about cytologic analysis in companion animal species Brings together authors from a wide range of specialties to present a thorough survey of cytology's use in veterinary medicine Offers broader species coverage and greater depth than any cytology reference currently available Veterinary Cytology is an essential resource for clinical and anatomic pathologists and any specialist in areas using cytology, including veterinary oncologists, criticalists, surgeons, ophthalmologists, dermatologists, and internists.
Diagnostic Techniques in Veterinary Dermatology by Ariane Neuber,Tim Nuttall Pdf
The first book devoted solely to the techniques used to investigate skin problems in animals A practical everyday reference for veterinary practitioners, Diagnostic Techniques in Veterinary Dermatology focuses on contemporary techniques for investigating skin problems in small animals, horses and exotic pets. Written by experienced specialists in veterinary dermatology, this book offers clear, step-by-step guidance on how to perform tests and interpret their results. The first book devoted exclusively to the subject, this hands-on guide demonstrates how to carry out and interpret a huge range of dermatology tests, as well as how to avoid common mistakes and pitfalls. Featuring full colour photographs and illustrations throughout, key topics include: looking for parasites, hair plucks and trichograms, dermoscopy, cytology, fungal and bacterial cultures, histopathology, allergy testing, immune-mediated skin diseases, endocrine and metabolic skin diseases, infectious diseases, diagnostic imaging, otoscopy and examination of the ear, genetic tests, and more. Diagnostic Techniques in Veterinary Dermatology is a valuable working resource for busy practitioners in first opinion practice, as well as veterinary nurses and technicians. It is also an ideal reference for veterinary students and specialists in-training.
Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies by Mónica V. Cunha,João Inácio Pdf
This volume provides an overview of molecular biology tools for pathogen detection and characterization. Focusing on biobanking, biosafety, good laboratory practices, biological specimen collection and processing, quality assurance and control, validation of molecular diagnostic assays for veterinary use; molecular detection and identification of animal pathogens using a wide range of established techniques and covering, also, emerging diagnosis approaches; genotyping tools for assessing the genetic landscape and epidemiology of pathogens; and integrative omics and high-throughput technologies as powerful research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies aids scientists in continuing to study a broad range of molecular approaches, from simple and affordable to emerging and sophisticate.
Interpretation of Equine Laboratory Diagnostics by Nicola Pusterla,Jill Higgins Pdf
Interpretation of Equine Laboratory Diagnostics offers a comprehensive approach to equine laboratory diagnostics, including hematology, clinical chemistry, serology, body fluid analysis, microbiology, clinical parasitology, endocrinology, immunology, and molecular diagnostics. Offers a practical resource for the accurate interpretation of laboratory results, with examples showing real-world applications Covers hematology, clinical chemistry, serology, body fluid analysis, microbiology, clinical parasitology, endocrinology, immunology, and molecular diagnostics Introduces the underlying principles of laboratory diagnostics Provides clinically oriented guidance on performing and interpreting laboratory tests Presents a complete reference to establish and new diagnostic procedures Offers a practical resource for the accurate interpretation of laboratory results, with examples showing real-world applications Covers hematology, clinical chemistry, serology, body fluid analysis, microbiology, clinical parasitology, endocrinology, immunology, and molecular diagnostics Introduces the underlying principles of laboratory diagnostics Provides clinically oriented guidance on performing and interpreting laboratory tests Presents a complete reference to established and new diagnostic procedures
Author : Victoria L. Cooper Publisher : Elsevier Health Sciences Page : 240 pages File Size : 50,7 Mb Release : 2012-11-14 Category : Medical ISBN : 9781455747979
Diagnostic Pathology, An Issue of Veterinary Clinics: Food Animal Practice - E-Book by Victoria L. Cooper Pdf
The latest information on diagnostic pathology for the food animal practitioner! Topics include respiratory diagnostics and pathology, diagnostics of dairy and beef calf diarrhea, gastrointestinal diagnostics and pathology in feedlot cattle, neuropathology and diagnostics in food animals, musculoskeletal and integument pathology in food animals, mastitis diagnostics, ruminant toxicology diagnostics, camelid pathology and diagnostics, abortion diagnostics, field necropsy and diagnostic sample submission, population approaches to diagnostics and epidemiology, and more!
Diagnostics and Therapy in Veterinary Dermatology by Dawn Logas Pdf
Diagnostics and Therapy in Veterinary Dermatology presents thorough coverage of the latest discoveries, drugs, and treatments for dermatologic conditions in animals. Chapters written by experts in each respective area of veterinary dermatology contain up-to-date information on new diagnostic tools and tests, autoimmune diseases, parasitic and fungal infections, medical management of acute and chronic conditions, alternative dermatologic therapies, and more. Offering practical solutions for both specialist and general practice veterinarians dealing with dermatology cases, this wide-ranging resource also addresses antibiotic resistance and misuse, the availability of foods for elimination diet trials, problems with generic drugs, emerging infectious diseases, and other important problems currently facing the profession. Throughout the text, veterinary practitioners are provided with real-world guidance on improving how they work up their dermatology cases and strengthening communication between the primary care veterinarian and the dermatologist. Edited by a leading board-certified dermatologist, this volume: Focuses on cats and dogs Includes numerous high-quality clinical photographs illustrating all key concepts Covers topics such as how to use your nursing staff to the fullest, the One Health movement, and how changing climate is increasing the spread of certain dermatologic diseases Discusses approaches for building a better working relationship between clients, primary care veterinarians and dermatologists Provides insights on the future of technology in the diagnosis and treatment of dermatologic diseases Covering the very latest developments in the field, Diagnostics and Therapy in Veterinary Dermatology is essential reading for veterinary dermatologists, veterinary students, and any veterinary general practitioner with a dermatology caseload.
Diagnostic Tools in Veterinary Medicine by Patricia Marques Pdf
The Key Components of Veterinary Diagnostic System are Vet Laboratory, Vet Devices and Veterinary Diagnostic Test Kits. Traditionally, laboratory diagnostics for veterinary pathogens have relied on methods of detecting the pathogen by culture or antibodies, using varied techniques, such as neutralization, Enzyme-linked Immunosorbent Assay (ELISA), agar gel immunodiffusion, and complement fixation. Over the years, veterinarians have incorporated new molecular diagnostic techniques, such as Polymerase Chain Reaction (PCR) and Western blot, as well as improved older techniques by using recombinant antigens, monoclonal antibodies, and synthetic peptides. Despite the routine use of conventional diagnostic assays, new molecular techniques have enabled veterinarians to use new tools for rapid and specific diagnosis of animal disease in real-time. The globalization of trade in animals and animal products is a major driver for the improvement of the analytical and diagnostic quality of tests.
Author : Mary Jo Burkhard,Maxey L. Wellman Publisher : Elsevier Health Sciences Page : 315 pages File Size : 46,5 Mb Release : 2013-11-28 Category : Medical ISBN : 9780323261395
Clinical Pathology and Diagnostic Testing, An Issue of Veterinary Clinics: Small Animal Practice by Mary Jo Burkhard,Maxey L. Wellman Pdf
A comprehensive volume on clinical pathology and diagnostic techniques for the small animal practitioner! Topics will include pancreatic testing, renal function testing, diagnosis of small intestinal disorders, hypocalcemia of critical illness, lactate in clinical practice, cardiac biomarkers, disorders of iron metabolism, lymphoma diagnostics, coagulation disorders, molecular approaches to the diagnosis of infectious diseases, hepatobiliary disease, and much more!
Canine and Feline Infectious Diseases - E-BOOK by Jane E. Sykes Pdf
Canine and Feline Infectious Diseases is a practical, up-to-date resource covering the most important and cutting-edge advances in the field. Presented by a seasoned educator in a concise, highly visual format, this innovative guide keeps you current with the latest advances in this ever-changing field. 80 case studies illustrate the clinical relevance of the major infectious disease chapters. Well-organized Major Infectious Diseases chapters break down content by etiologic agent and epidemiology, clinical signs and their pathophysiology, physical examination findings, diagnosis, treatment and prognosis, immunity, prevention, and public health implications. Over 80 case studies illustrate how the information provided can be applied in everyday practice. Logical approach to laboratory diagnosis guides you through all the steps needed to accurately diagnose and treat viral, bacterial, fungal, protozoal, and algal diseases. Practical protocols provided by expert clinicians guide you in the management of canine and feline patients suspected to have infectious diseases, including handling, disinfection, isolation, and vaccination protocols. Over 500 full color images – geographic distribution maps, life cycle drawings, and hundreds of color photographs – visually illustrate and clarify complex issues. Easy-to-understand tables and boxes make content quickly accessible, eliminating the need to sort through dense text for critical information in the clinical setting.
