Video Microscopy

Author : Shinya Inoue
Publisher : Springer Science & Business Media
Page : 600 pages
File Size : 55,5 Mb
Release : 2013-11-11
Category : Medical
ISBN : 9781475769258

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Video Microscopy by Shinya Inoue Pdf

Ever since television became practical in the early 1950s, closed-circuit television (CCTV) in conjunction with the light microscope has provided large screen display, raised image contrast, and made the images formed by ultraviolet and infrared rays visible. With the introduction of large-scale integrated circuits in the last decade, TV equipment has improved by leaps and bounds, as has its application in microscopy. With modem CCTV, sometimes with the help of digital computers, we can distill the image from a scene that appears to be nothing but noise; capture fluorescence too dim to be seen; visualize structures far below the limit of resolution; crispen images hidden in fog; measure, count, and sort objects; and record in time-lapsed and high-speed sequences through the light microscope without great difficulty. In fact, video is becoming indispensable for harnessing the fullest capacity of the light microscope, a capacity that itself is much greater than could have been envisioned just a few years ago. The time seemed ripe then to review the basics of video, and of microscopy, and to examine how the two could best be combined to accomplish these tasks. The Marine Biological Laboratory short courses on Analytical and Quantitative Light Microscopy in Biology, Medicine, and the Materials Sciences, and the many inquiries I received on video microscopy, supported such an effort, and Kirk Jensen of Plenum Press persuaded me of its worth.

Author : Randy O. Wayne
Publisher : Academic Press
Page : 366 pages
File Size : 43,8 Mb
Release : 2013-12-16
Category : Science
ISBN : 9780124115361

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Light and Video Microscopy by Randy O. Wayne Pdf

The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope Clearly develops all ideas from historical and logical foundations Laboratory exercises included to assist the reader with practical applications Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope

Author : Anonim
Publisher : Academic Press
Page : 334 pages
File Size : 52,5 Mb
Release : 1998-04-10
Category : Science
ISBN : 0080859542

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Video Microscopy by Anonim Pdf

Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques. Practical laboratory guide for methods and technologies used with video microscopy Comprehensive, easy-to-follow instructions February 1998, c. 334 pp.

Author : Shinya Inoué,Kenneth R. Spring
Publisher : Springer
Page : 742 pages
File Size : 49,5 Mb
Release : 1997-08-31
Category : Medical
ISBN : 0306455315

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Video Microscopy by Shinya Inoué,Kenneth R. Spring Pdf

The current edition of Video Microscopy has been totally revised to reflect the advances in the tools for electronic imaging, processing, recording, and analysis, as well as applications that are being made in video microscopy and related modes of electronic imaging. The less spiral organization of the revised edition reflects an audience expected to be more experi enced in video and computer image processing than in the earlier days when coupling of video equipment and computers to the light microscope was still considered to be a novelty. Nevertheless, we have emphasi2;ed the how-tos, as well as the fundamental principles, involved in imaging and processing ih the light microscope, the human visual system, video and related electronic imaging, and the digital image processor in the hope that the reader will develop enough understanding, not only to apply rationally what is available, but also to contribute actively to the development of this evolving field. In the text, the terms appearing in the Glossary are printed in bold type, generally, at first appearance. Italics are used for emphasis.

Author : Anonim
Publisher : Academic Press
Page : 561 pages
File Size : 52,8 Mb
Release : 2003-12-18
Category : Computers
ISBN : 9780080546032

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Digital Microscopy by Anonim Pdf

This updated second edition of the popular methods book "Video Microscopy" shows how to track dynamic changes in the structure or architecture of living cells and in reconstituted preparations using video and digital imaging microscopy. Contains 10 new chapters addressing developments over the last several years. Basic information, principles, applications, and equipment are covered in the first half of the volume and more spcialized video microscopy techniques are covered in the second half. Shows how to track dynamic changes in the structure or architecture of living cells and in reconstituted preparations using video and digital imaging microscopy Contains 10 new chapters addressing developments over the last several years Covers basic principles, applications, and equipment Spcialized video microscopy techniques are covered

Author : Leslie Wilson,Greenfield Sluder,Paul T. Matsudaira,David E. Wolf
Publisher : Gulf Professional Publishing
Page : 362 pages
File Size : 51,6 Mb
Release : 1998-02-26
Category : Computers
ISBN : 0126491607

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Video Microscopy by Leslie Wilson,Greenfield Sluder,Paul T. Matsudaira,David E. Wolf Pdf

Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques. Chapters cover the commonly used video technologies in biological research Nontechnical presentation of principles Emphasis on the practical aspects of instrument use Covers pitfalls in instrument uses that can lead to artifacts Authors are leaders in the design and application of video methods to biological microscopy Presentation of material tailored for the established researcher that has little experience with video methods Examples are extensively illustrated with photographs

Author : James Pawley
Publisher : Springer Science & Business Media
Page : 639 pages
File Size : 50,8 Mb
Release : 2013-04-17
Category : Science
ISBN : 9781475753486

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Handbook of Biological Confocal Microscopy by James Pawley Pdf

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

Author : Randy O. Wayne
Publisher : Academic Press
Page : 518 pages
File Size : 40,6 Mb
Release : 2019-06-11
Category : Technology & Engineering
ISBN : 9780128161678

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Light and Video Microscopy by Randy O. Wayne Pdf

Light and Video Microscopy, Third Edition provides a step-by-step journey through philosophy, psychology and the geometrical and physical optics involved in interpreting images formed by light microscopes. The book addresses the intricacies necessary to set up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine various physico-chemical properties of specimens. This updated edition includes the most recent developments in microscopy, ensuring that it continues to be the most comprehensive, easy-to-use, and informative guide on light microscopy. With its presentation of geometrical optics, it assists the reader in understanding image formation and light movement within the microscope. Provides a fully-revised, updated resource on three-dimensional (3D) structures Contains a new appendices on Diffraction Theory and Advanced Image Processing Provides practical applications, lab exercises and case studies on the mathematics, physics and biology used in microscopy Discusses bright field, dark field, phase-contrast, fluorescence, interference, differential interference and modulation contrast microscopes, oblique illumination and photomicrography

Author : Robert W. Ogilvie
Publisher : CRC Press
Page : 376 pages
File Size : 40,6 Mb
Release : 2005-06-22
Category : Medical
ISBN : 9781420039306

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Virtual Microscopy and Virtual Slides in Teaching, Diagnosis, and Research by Robert W. Ogilvie Pdf

Despite a brief history, the technologies of virtual microscopy and virtual slides have captured the imagination of many, especially this current crop of students. Having come of age in the computer and Internet age, this emerging group of technicians and researchers tends to display a distinct preference for virtual slides and virtual microscopes.

Author : Alan J. Lacey
Publisher : OUP Oxford
Page : 475 pages
File Size : 47,9 Mb
Release : 1999-06-03
Category : Science
ISBN : 9780191565724

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Light Microscopy in Biology by Alan J. Lacey Pdf

Since the first edition of Light Microscopy in Biology: A Practical approach was published, techniques in modern light microscopy have improved considerably. This fully updated edition includes revised topics from the first edition as well as coverage of techniques and technologies that have been developed since it was published. As before, the book starts with an explanation of the basic techniques, and goes on to describe current methods in: chromosome microscopy, immunohistochemistry, fluorescence microscopy, image building and video microscopy. Totally new topics covered include: confocal microscopy, calcium and pH imaging, microinjection techniques and nanovid microscopy. There are also whole chapters now devoted to reflection contrast microscopy and histomorphometry. This new edition will be of great interest to postgraduate and postdoctoral researchers in biomedicine and cell biology - both those experienced with light microscopic techniques and newcomers to the field.

Author : Greenfield Sluder,David E. Wolf
Publisher : Elsevier
Page : 632 pages
File Size : 47,8 Mb
Release : 2007-04-26
Category : Science
ISBN : 0080544347

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Digital Microscopy by Greenfield Sluder,David E. Wolf Pdf

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. * Expands coverage to include discussion of confocal microscopy not found in the previous edition * Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

Author : G. Sluder
Publisher : Unknown
Page : 0 pages
File Size : 46,5 Mb
Release : 1998
Category : Electronic
ISBN : OCLC:708392888

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Video Microscopy by G. Sluder Pdf

Author : Vasily Astratov
Publisher : Springer Nature
Page : 487 pages
File Size : 42,7 Mb
Release : 2019-08-31
Category : Science
ISBN : 9783030217228

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Label-Free Super-Resolution Microscopy by Vasily Astratov Pdf

This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.

Author : Anonim
Publisher : Elsevier
Page : 507 pages
File Size : 41,5 Mb
Release : 2003-01-04
Category : Science
ISBN : 9780080496580

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Cell Biological Applications of Confocal Microscopy by Anonim Pdf

This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures

Author : F. W. D. Rost
Publisher : Cambridge University Press
Page : 264 pages
File Size : 52,7 Mb
Release : 1991-07-26
Category : Science
ISBN : 0521394228

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Quantitative Fluorescence Microscopy by F. W. D. Rost Pdf

Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.