A Laboratory Manual Of Analytical Methods Of Protein Chemistry Including Polypeptides Amino Acid Analysis Of Protein Hydrolysates

Author : Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren
Publisher : Unknown
Page : 566 pages
File Size : 48,5 Mb
Release : 1960
Category : Analytical chemistry
ISBN : UCAL:B4165319

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A Laboratory Manual of Analytical Methods of Protein Chemistry, Including Polypeptides: Amino acid analysis of protein hydrolysates by Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren Pdf

In the last fifteen years there has been a revolution in the techniques available for the analysis and isolation of proteins. Every time a new technique has been introduced, numerous papers have appeared describing modifications to it and the research worker who wishes to employ these methods is faced with a very serious problem in deciding which particular variant to use. These volumes are intended to provide the fullest practical detail so that any scientist can follow the procedure by using this book alone and without having recourse to the original literature. The techniques which are described in full are ones in which all the authors have had first-hand experience, and the descriptions contain those small but important points which save so much time. In the first volume, separation and isolation procedures are discussed; the second concerns its analysis and reactivity, and the third volume with the measurement of the macromolecular properties of proteins.

Author : P. Alexander,H. P. Lundgren
Publisher : Elsevier
Page : 256 pages
File Size : 46,9 Mb
Release : 2014-05-09
Category : Science
ISBN : 9781483185644

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A Laboratory Manual of Analytical Methods of Protein Chemistry by P. Alexander,H. P. Lundgren Pdf

A Laboratory Manual of Analytical Methods of Protein Chemistry, Volume 4 provides information pertinent to the fundamental aspects of protein chemistry. This book discusses the simple and accurate methods of estimating specific proteins. Organized into six chapters, this volume begins with an overview of the composition of acids and experimental conditions for the acid hydrolysis of proteins. This text then examines the advantages of high-voltage electrophoresis for amino acid analysis, which are paralleled by equal advantages in the peptide separation field. Other chapters consider the simple technique of estimating specific proteins, which is one of several based on the phenomenon of antigen-antibody precipitation in gels. This book discusses as well the summations of analyses in weight percentages of the various residues and of the nitrogen of each constituent. The final chapter deals with the electrical properties of molecules. This book is a valuable resource for physicists and research workers.

Author : Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren
Publisher : Unknown
Page : 298 pages
File Size : 45,6 Mb
Release : 1960
Category : Analytical chemistry
ISBN : UOM:39015017271381

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A Laboratory Manual of Analytical Methods of Protein Chemistry, Including Polypeptides by Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren Pdf

In the last fifteen years there has been a revolution in the techniques available for the analysis and isolation of proteins. Every time a new technique has been introduced, numerous papers have appeared describing modifications to it and the research worker who wishes to employ these methods is faced with a very serious problem in deciding which particular variant to use. These volumes are intended to provide the fullest practical detail so that any scientist can follow the procedure by using this book alone and without having recourse to the original literature. The techniques which are described in full are ones in which all the authors have had first-hand experience, and the descriptions contain those small but important points which save so much time. In the first volume, separation and isolation procedures are discussed; the second concerns its analysis and reactivity, and the third volume with the measurement of the macromolecular properties of proteins.

Author : P. Alexander,R. J. Block
Publisher : Elsevier
Page : 289 pages
File Size : 49,7 Mb
Release : 2014-05-15
Category : Science
ISBN : 9781483149806

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The Separation and Isolation of Proteins by P. Alexander,R. J. Block Pdf

A Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides), Volume 1: The Separation and Isolation of Proteins deals with the techniques used in the separation and isolation of proteins, including fractionation and characterization by dialysis, multi-membrane electrodecantation, and zonal density gradient electrophoresis. The fractionation of proteins by adsorption and ion exchange is also described. This book is comprised of seven chapters and begins with a discussion on procedures for the separation of proteins, paying particular attention to the liberation of proteins from cellular material; removal of lipids from lipoproteins; and denaturation, fractionation, and purification of proteins. The next chapter focuses on the isolation of biologically active proteins such as cytochrome, bacterial amylases, and bacterial proteinases. The reader is methodically introduced to fractionation of proteins by adsorption and ion exchange; fractionation and characterization by dialysis; multi-membrane electrodecantation; and continuous and discontinuous partition. The final chapter explains how zonal density gradient electrophoresis works as a separation method for natural mixtures of proteins, their degradation products, and other substances carrying electric charges in solution or suspension. This volume will be of interest to chemists working with proteins.

Author : P. Alexander,H. P. Lundgren
Publisher : Elsevier
Page : 265 pages
File Size : 48,6 Mb
Release : 2014-05-17
Category : Science
ISBN : 9781483152455

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A Laboratory Manual of Analytical Methods of Protein Chemistry by P. Alexander,H. P. Lundgren Pdf

A Laboratory Manual of Analytical Methods of Protein Chemistry, Volume 5 presents the laboratory techniques for protein and polypeptide study. This book discusses the staining procedure for histones, which has a high degree of selectivity for basic proteins and the unique ability to visualize qualitative differences in terms of color changes. Organized into four chapters, this volume begins with an overview of the formalin-mediated ammoniacal-silver staining procedure as a selective stain for basic proteins and its application per cell and per extract. This text then examines the optical rotatory dispersion (ORD), which has advanced into a powerful tool for describing the conformations and conformational changes of biopolymers. Other chapters consider the application of ultrasensitive calorimetry to thermodynamic problems. This book discusses as well the principle of the technique, its instrumentation, and experimental procedures. The final chapter deals with the hydrodynamic densities and preferential hydration values for protein precipitates in concentrated salt solutions. This book is a valuable resource for chemists and biochemists.

Author : P. Alexander,R. J. Block
Publisher : Elsevier
Page : 554 pages
File Size : 49,9 Mb
Release : 2014-06-05
Category : Science
ISBN : 9781483184531

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The Composition, Structure and Reactivity of Proteins by P. Alexander,R. J. Block Pdf

The Composition, Structure and Reactivity of Proteins

Author : P. Alexander,R. J. Block
Publisher : Elsevier
Page : 309 pages
File Size : 52,5 Mb
Release : 2014-05-12
Category : Science
ISBN : 9781483221281

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Determination of the Size and Shape of Protein Molecules by P. Alexander,R. J. Block Pdf

Analytical Methods of Protein Chemistry, Volume 3: Determination of the Size and Shape of Protein Molecules provides information pertinent to the analysis and isolation of protein. This book deals with the measurement of the macromolecular properties of proteins. Organized into seven chapters, this volume begins with an overview of the theory and practice of the electron microscope to allow an understanding of the type of object that may be examined. This text then describes the methods of making protein molecules conform to such an ideal, which are the techniques of specimen preparation. Other chapters consider the determinations of osmotic pressures of proteins. This book discusses as well the experimental basis for the theory of the diffusion process in liquids. The final chapter deals with the technical problem characteristics of light-scattering. This book is a valuable resource for electron microscopists, protein chemists, biologists, physicist, physico-chemists, scientists, and research workers.

Author : Peter Alexander
Publisher : Unknown
Page : 233 pages
File Size : 54,7 Mb
Release : 1966
Category : Electronic
ISBN : OCLC:925672840

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A Laboratory Manual of Analytical Methods of Protein Chemistry Including Polypeptides by Peter Alexander Pdf

Author : Peter Alexander,Richard Joseph Block
Publisher : Unknown
Page : 128 pages
File Size : 45,6 Mb
Release : 1960
Category : Proteins
ISBN : OCLC:893973586

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A Laboratory Manual of Analytical Methods of Protein Chemistry (including Peptides). by Peter Alexander,Richard Joseph Block Pdf

Author : Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren
Publisher : Unknown
Page : 558 pages
File Size : 48,9 Mb
Release : 1960
Category : Analytical chemistry
ISBN : UCLA:L0065421661

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A Laboratory Manual of Analytical Methods of Protein Chemistry, Including Polypeptides: Amino acid analysis of protein hydrolysates by Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren Pdf

In the last fifteen years there has been a revolution in the techniques available for the analysis and isolation of proteins. Every time a new technique has been introduced, numerous papers have appeared describing modifications to it and the research worker who wishes to employ these methods is faced with a very serious problem in deciding which particular variant to use. These volumes are intended to provide the fullest practical detail so that any scientist can follow the procedure by using this book alone and without having recourse to the original literature. The techniques which are described in full are ones in which all the authors have had first-hand experience, and the descriptions contain those small but important points which save so much time. In the first volume, separation and isolation procedures are discussed; the second concerns its analysis and reactivity, and the third volume with the measurement of the macromolecular properties of proteins.

Author : P. Alexander
Publisher : Unknown
Page : 128 pages
File Size : 48,9 Mb
Release : 1960
Category : Electronic
ISBN : OCLC:966830698

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A Laboratory Manual of Analytical Methods of Protein Chemistry by P. Alexander Pdf

Author : J.P. Miksche
Publisher : Springer Science & Business Media
Page : 303 pages
File Size : 40,8 Mb
Release : 2013-03-09
Category : Technology & Engineering
ISBN : 9783662125236

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Modern Methods in Forest Genetics by J.P. Miksche Pdf

The present volume contains papers developed from courses given at the International Union of Forest Research Organizations (IUFRO) Bio chemical Genetics Workshop (Working Party S.04-5) held at the Univer sity of Gottingen, Germany on July 5 through 28, 1973. The workshop was organized by Professor Robert G. Stanley and was held in memory of Professor Klaus Stern. Unfortunately, both met with untimely deaths. Professor Stanley was also instrumental in initiating the process of having the workshop proceedings published. I was asked by the workshop participants to complete this task, and I wish to acknowledge their cooperation, advice and encouragement. In addition to the courses and subsequent papers resulting from the above workshop, we have included some papers by colleagues who were unable to attend the meeting. The contents of this text may, there fore, be considered a working-manual of generally "modern" techniques that are applicable to forest genetics and breeding programs. The chapters are placed in five major categories. The first three categories follow according to classes of chemical constituents in herent to plants which are nucleic acids (DNA, RNA) , primary gene products (amino acids, proteins and enzymes) and primary and secon dary metabolites (carbohydrate polymers, resins, phenolics, pigments, etc.). The fourth category is concerned with the interaction of en vironment and gene systems. Indirect selection, crossing and proto plasmic and flowering manipulation are factors covered in the fifth category.

Author : Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren
Publisher : Unknown
Page : 316 pages
File Size : 46,6 Mb
Release : 1960
Category : Analytical chemistry
ISBN : UOM:39015072139267

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A Laboratory Manual of Analytical Methods of Protein Chemistry, Including Polypeptides: Determination of the size and shape of protein molecules by Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren Pdf

In the last fifteen years there has been a revolution in the techniques available for the analysis and isolation of proteins. Every time a new technique has been introduced, numerous papers have appeared describing modifications to it and the research worker who wishes to employ these methods is faced with a very serious problem in deciding which particular variant to use. These volumes are intended to provide the fullest practical detail so that any scientist can follow the procedure by using this book alone and without having recourse to the original literature. The techniques which are described in full are ones in which all the authors have had first-hand experience, and the descriptions contain those small but important points which save so much time. In the first volume, separation and isolation procedures are discussed; the second concerns its analysis and reactivity, and the third volume with the measurement of the macromolecular properties of proteins.

Author : Robert A. Copeland
Publisher : Springer Science & Business Media
Page : 238 pages
File Size : 47,5 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781475715057

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Methods for Protein Analysis by Robert A. Copeland Pdf

As protein science continues to become an increasingly important aspect of academic and commercial sciences and technology, the need has arisen for a ready source of laboratory protocols for the analysis and evaluation of these biological polymers. Methods for Protein Analysis presents the methods most relevant to the generalist bench scientist working with proteins. A concise yet thorough summary, it covers laboratory methods that can be reasonably performed in a standard protein laboratory, without specialized equipment or expertise. Taking a how to approach, this book examines the techniques used to answer common protein analytical questions and describes methods useful in daily laboratory work. Methods for Protein Analysis is the ideal reference for protein laboratories in academic, government and industrial settings. It is an essential benchtop manual for first-year graduate students beginning their laboratory experience as well as for chemists, biochemists, and molecular biologists in the pharmaceutical, biotechnological, food and specialty chemical industries, and for analysts concerned with the purity and structural integrity of protein. Featuring illustrations and a convenient spiral binding, this guide offers a glossary of common abbreviations and a list of suppliers for protein science.

Author : Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren
Publisher : Unknown
Page : 128 pages
File Size : 54,7 Mb
Release : 1960
Category : Chemistry, Analytic
ISBN : OCLC:26254065

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A Laboratory Manual of Analytical Methods of Protein Chemistry by Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren Pdf