A Laboratory Manual Of Analytical Methods Of Protein Chemistry

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The Separation and Isolation of Proteins

P. Alexander,R. J. Block
The Separation and Isolation of Proteins Book PDF

Author : P. Alexander,R. J. Block
Publisher : Elsevier
Page : 289 pages
File Size : 55,5 Mb
Release : 2014-05-15
Category : Science
ISBN : 9781483149806

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The Separation and Isolation of Proteins by P. Alexander,R. J. Block Pdf

A Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides), Volume 1: The Separation and Isolation of Proteins deals with the techniques used in the separation and isolation of proteins, including fractionation and characterization by dialysis, multi-membrane electrodecantation, and zonal density gradient electrophoresis. The fractionation of proteins by adsorption and ion exchange is also described. This book is comprised of seven chapters and begins with a discussion on procedures for the separation of proteins, paying particular attention to the liberation of proteins from cellular material; removal of lipids from lipoproteins; and denaturation, fractionation, and purification of proteins. The next chapter focuses on the isolation of biologically active proteins such as cytochrome, bacterial amylases, and bacterial proteinases. The reader is methodically introduced to fractionation of proteins by adsorption and ion exchange; fractionation and characterization by dialysis; multi-membrane electrodecantation; and continuous and discontinuous partition. The final chapter explains how zonal density gradient electrophoresis works as a separation method for natural mixtures of proteins, their degradation products, and other substances carrying electric charges in solution or suspension. This volume will be of interest to chemists working with proteins.

A Laboratory Manual of Analytical Methods of Protein Chemistry

P. Alexander,H. P. Lundgren
A Laboratory Manual of Analytical Methods of Protein Chemistry Book PDF

Author : P. Alexander,H. P. Lundgren
Publisher : Elsevier
Page : 256 pages
File Size : 40,5 Mb
Release : 2014-05-09
Category : Science
ISBN : 9781483185644

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A Laboratory Manual of Analytical Methods of Protein Chemistry by P. Alexander,H. P. Lundgren Pdf

A Laboratory Manual of Analytical Methods of Protein Chemistry, Volume 4 provides information pertinent to the fundamental aspects of protein chemistry. This book discusses the simple and accurate methods of estimating specific proteins. Organized into six chapters, this volume begins with an overview of the composition of acids and experimental conditions for the acid hydrolysis of proteins. This text then examines the advantages of high-voltage electrophoresis for amino acid analysis, which are paralleled by equal advantages in the peptide separation field. Other chapters consider the simple technique of estimating specific proteins, which is one of several based on the phenomenon of antigen-antibody precipitation in gels. This book discusses as well the summations of analyses in weight percentages of the various residues and of the nitrogen of each constituent. The final chapter deals with the electrical properties of molecules. This book is a valuable resource for physicists and research workers.

A Laboratory Manual of Analytical Methods of Protein Chemistry

Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren
A Laboratory Manual of Analytical Methods of Protein Chemistry Book PDF

Author : Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren
Publisher : Unknown
Page : 128 pages
File Size : 50,6 Mb
Release : 1960
Category : Chemistry, Analytic
ISBN : OCLC:26254065

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A Laboratory Manual of Analytical Methods of Protein Chemistry by Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren Pdf

A Laboratory Manual of Analytical Methods of Protein Chemistry

P. Alexander,H. P. Lundgren
A Laboratory Manual of Analytical Methods of Protein Chemistry Book PDF

Author : P. Alexander,H. P. Lundgren
Publisher : Elsevier
Page : 265 pages
File Size : 51,8 Mb
Release : 2014-05-17
Category : Science
ISBN : 9781483152455

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A Laboratory Manual of Analytical Methods of Protein Chemistry by P. Alexander,H. P. Lundgren Pdf

A Laboratory Manual of Analytical Methods of Protein Chemistry, Volume 5 presents the laboratory techniques for protein and polypeptide study. This book discusses the staining procedure for histones, which has a high degree of selectivity for basic proteins and the unique ability to visualize qualitative differences in terms of color changes. Organized into four chapters, this volume begins with an overview of the formalin-mediated ammoniacal-silver staining procedure as a selective stain for basic proteins and its application per cell and per extract. This text then examines the optical rotatory dispersion (ORD), which has advanced into a powerful tool for describing the conformations and conformational changes of biopolymers. Other chapters consider the application of ultrasensitive calorimetry to thermodynamic problems. This book discusses as well the principle of the technique, its instrumentation, and experimental procedures. The final chapter deals with the hydrodynamic densities and preferential hydration values for protein precipitates in concentrated salt solutions. This book is a valuable resource for chemists and biochemists.

A Laboratory Manual of Analytical Methods of Protein Chemistry, Including Polypeptides

Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren
A Laboratory Manual of Analytical Methods of Protein Chemistry Including Polypeptides Book PDF

Author : Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren
Publisher : Unknown
Page : 266 pages
File Size : 53,7 Mb
Release : 1960
Category : Analytical chemistry
ISBN : UOM:39015004617539

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A Laboratory Manual of Analytical Methods of Protein Chemistry, Including Polypeptides by Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren Pdf

In the last fifteen years there has been a revolution in the techniques available for the analysis and isolation of proteins. Every time a new technique has been introduced, numerous papers have appeared describing modifications to it and the research worker who wishes to employ these methods is faced with a very serious problem in deciding which particular variant to use. These volumes are intended to provide the fullest practical detail so that any scientist can follow the procedure by using this book alone and without having recourse to the original literature. The techniques which are described in full are ones in which all the authors have had first-hand experience, and the descriptions contain those small but important points which save so much time. In the first volume, separation and isolation procedures are discussed; the second concerns its analysis and reactivity, and the third volume with the measurement of the macromolecular properties of proteins.

The Composition, Structure and Reactivity of Proteins

P. Alexander,R. J. Block
The Composition Structure and Reactivity of Proteins Book PDF

Author : P. Alexander,R. J. Block
Publisher : Elsevier
Page : 554 pages
File Size : 52,9 Mb
Release : 2014-06-05
Category : Science
ISBN : 9781483184531

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The Composition, Structure and Reactivity of Proteins by P. Alexander,R. J. Block Pdf

The Composition, Structure and Reactivity of Proteins

A Laboratory Manual of Analytical Methods of Protein Chemistry, Including Polypeptides: Amino acid analysis of protein hydrolysates

Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren
A Laboratory Manual of Analytical Methods of Protein Chemistry Including Polypeptides Amino acid analysis of protein hydrolysates Book PDF

Author : Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren
Publisher : Unknown
Page : 564 pages
File Size : 44,5 Mb
Release : 1960
Category : Analytical chemistry
ISBN : UOM:39015015993580

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A Laboratory Manual of Analytical Methods of Protein Chemistry, Including Polypeptides: Amino acid analysis of protein hydrolysates by Peter Alexander,Richard Joseph Block,Harold Palmer Lundgren Pdf

In the last fifteen years there has been a revolution in the techniques available for the analysis and isolation of proteins. Every time a new technique has been introduced, numerous papers have appeared describing modifications to it and the research worker who wishes to employ these methods is faced with a very serious problem in deciding which particular variant to use. These volumes are intended to provide the fullest practical detail so that any scientist can follow the procedure by using this book alone and without having recourse to the original literature. The techniques which are described in full are ones in which all the authors have had first-hand experience, and the descriptions contain those small but important points which save so much time. In the first volume, separation and isolation procedures are discussed; the second concerns its analysis and reactivity, and the third volume with the measurement of the macromolecular properties of proteins.

Determination of the Size and Shape of Protein Molecules

P. Alexander,R. J. Block
Determination of the Size and Shape of Protein Molecules Book PDF

Author : P. Alexander,R. J. Block
Publisher : Elsevier
Page : 309 pages
File Size : 43,5 Mb
Release : 2014-05-12
Category : Science
ISBN : 9781483221281

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Determination of the Size and Shape of Protein Molecules by P. Alexander,R. J. Block Pdf

Analytical Methods of Protein Chemistry, Volume 3: Determination of the Size and Shape of Protein Molecules provides information pertinent to the analysis and isolation of protein. This book deals with the measurement of the macromolecular properties of proteins. Organized into seven chapters, this volume begins with an overview of the theory and practice of the electron microscope to allow an understanding of the type of object that may be examined. This text then describes the methods of making protein molecules conform to such an ideal, which are the techniques of specimen preparation. Other chapters consider the determinations of osmotic pressures of proteins. This book discusses as well the experimental basis for the theory of the diffusion process in liquids. The final chapter deals with the technical problem characteristics of light-scattering. This book is a valuable resource for electron microscopists, protein chemists, biologists, physicist, physico-chemists, scientists, and research workers.

Separation and Isolation of Proteins

Peter Alexander,Alexander R. J. Block,R. Block
Separation and Isolation of Proteins Book PDF

Author : Peter Alexander,Alexander R. J. Block,R. Block
Publisher : Pergamon
Page : 254 pages
File Size : 52,7 Mb
Release : 1960
Category : Electronic
ISBN : 008009256X

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Separation and Isolation of Proteins by Peter Alexander,Alexander R. J. Block,R. Block Pdf

Proteins and Proteomics

Richard J. Simpson
Proteins and Proteomics Book PDF

Author : Richard J. Simpson
Publisher : CSHL Press
Page : 946 pages
File Size : 49,7 Mb
Release : 2003
Category : Proteins
ISBN : 0879695544

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Proteins and Proteomics by Richard J. Simpson Pdf

Introduction to proteomics; one-dimensional polyacrylamide gel electrophoresis; preparing cellular and subcellular extracts; preparative two-dimensional gel electrophoresis with immobilized pH gradients; reversed-phase high -performance liquid chromatography; amino- and carboxy-terminal sequence analysis; peptide mapping and sequence analysis of gel-resolved proteins; the use of mass spectrometry in proteomics; proteomic methods for phosphorylation site mapping; characterization of protein complexes; making sense of proteomics - using bioinformatics to discover a protein's structure, functions, and interactions.