Advances In New Technology For Targeted Modification Of Plant Genomes

Author : Feng Zhang,Holger Puchta,James G. Thomson
Publisher : Springer
Page : 166 pages
File Size : 49,6 Mb
Release : 2015-04-21
Category : Science
ISBN : 9781493925568

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Advances in New Technology for Targeted Modification of Plant Genomes by Feng Zhang,Holger Puchta,James G. Thomson Pdf

Over the past 50 years, biotechnology has been the major driving force for increasing crop productivity. Particularly, advances in plant genetic engineering technologies have opened up vast new opportunities for plant researchers and breeders to create new crop varieties with desirable traits. Recent development of precise genome modification methods, such as targeted gene knock-out/knock-in and precise gene replacement, moves genetic engineering to another level and offers even more potentials for improving crop production. The work provides an overview of the latest advances on precise genomic engineering technologies in plants. Topics include recombinase and engineered nucleases-mediated targeted modification, negative/positive selection-based homologous recombination and oligo nucleotide-mediated recombination. Finally, challenges and impacts of the new technologies on present regulations for genetic modification organisms (GMOs) will be discussed.

Author : Shabir Hussain Wani,Goetz Hensel
Publisher : Springer Nature
Page : 338 pages
File Size : 43,9 Mb
Release : 2022-11-08
Category : Science
ISBN : 9783031080722

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Genome Editing by Shabir Hussain Wani,Goetz Hensel Pdf

Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : Jen-Tsung Chen
Publisher : Springer Nature
Page : 316 pages
File Size : 49,7 Mb
Release : 2024-06-28
Category : Electronic
ISBN : 9789819993383

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Plant Genome Editing Technologies by Jen-Tsung Chen Pdf

Author : National Academies of Sciences, Engineering, and Medicine,Division on Earth and Life Studies,Board on Agriculture and Natural Resources,Committee on Genetically Engineered Crops: Past Experience and Future Prospects
Publisher : National Academies Press
Page : 607 pages
File Size : 45,9 Mb
Release : 2017-01-28
Category : Science
ISBN : 9780309437387

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Genetically Engineered Crops by National Academies of Sciences, Engineering, and Medicine,Division on Earth and Life Studies,Board on Agriculture and Natural Resources,Committee on Genetically Engineered Crops: Past Experience and Future Prospects Pdf

Genetically engineered (GE) crops were first introduced commercially in the 1990s. After two decades of production, some groups and individuals remain critical of the technology based on their concerns about possible adverse effects on human health, the environment, and ethical considerations. At the same time, others are concerned that the technology is not reaching its potential to improve human health and the environment because of stringent regulations and reduced public funding to develop products offering more benefits to society. While the debate about these and other questions related to the genetic engineering techniques of the first 20 years goes on, emerging genetic-engineering technologies are adding new complexities to the conversation. Genetically Engineered Crops builds on previous related Academies reports published between 1987 and 2010 by undertaking a retrospective examination of the purported positive and adverse effects of GE crops and to anticipate what emerging genetic-engineering technologies hold for the future. This report indicates where there are uncertainties about the economic, agronomic, health, safety, or other impacts of GE crops and food, and makes recommendations to fill gaps in safety assessments, increase regulatory clarity, and improve innovations in and access to GE technology.

Author : Kasi Azhakanandam,Aron Silverstone,Henry Daniell,Michael R. Davey
Publisher : Springer
Page : 455 pages
File Size : 48,8 Mb
Release : 2015-03-03
Category : Science
ISBN : 9781493922024

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Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants by Kasi Azhakanandam,Aron Silverstone,Henry Daniell,Michael R. Davey Pdf

In this book, authors who are experts in their fields describe current advances on commercial crops and key enabling technologies that will underpin future advances in biotechnology. They discuss state of the art discoveries as well as future challenges. Tremendous progress has been made in introducing novel genes and traits into plant genomes since the first creation of transgenic plants thirty years ago, and the first commercialization of genetically modified maize in 1996. Consequently, cultivation of biotech crops with useful traits has increased more than 100-fold from 1.7 million hectares in 1996 to over 175 million hectares globally in 2013. This achievement has been made possible by continued advances in understanding the basic molecular biology of regulatory sequences to modulate gene expression, enhancement of protein synthesis and new technologies for transformation of crop plants. This book has three sections that encompass knowledge on genetically modified (GM) food crops that are currently used by consumers, those that are anticipated to reach the market place in the near future and enabling technologies that will facilitate the development of next generation GM crops. Section I focuses only on genetically modified maize and soybean (3 chapters each), while Section II discusses the GM food crops rice, wheat, sorghum, vegetables and sugar cane. Section III covers exciting recent developments in several novel enabling technologies, including gene targeting, minichromosomes, and in planta transient expression systems.

Author : Bidyut Kumar Sarmah,Basanta Kumar Borah
Publisher : Springer Nature
Page : 277 pages
File Size : 44,9 Mb
Release : 2021-04-18
Category : Science
ISBN : 9783030633721

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Genome Engineering for Crop Improvement by Bidyut Kumar Sarmah,Basanta Kumar Borah Pdf

This book serves the teachers, researchers and the students as a handy and concise reference as well as guidebook while designing and planning for use of the advanced technologies for crop improvement. The content of the book is designed to cover the latest genome engineering techniques for crop improvement. The conventional breeding has got its limitations such as non-availability of desired genes within the genepool. In many cases, breeding has been highly used and it has nearly reached its highest limit so far as the productivity and production of crops are concerned. However, with increasing need of food and decreasing resources, including water, land, labour, etc., to feed the growing population, the alternative available ways of increasing crop productivity need to be explored and exploited. Genome engineering has a wide scope that includes technologies such as genetic engineering and transgenesis, RNA technologies, CRISPR, cisgenics and subgenics for better productivity and more efficient biotic and abiotic stress management. Therefore, the book is planned to enlighten the readers with the advanced technologies with examples and case studies, whenever possible. Efforts will be made to emphasize on general efforts on various major food crops; however, it would also be made clear that such efforts could be taken as proofs of concepts and that this could be extrapolated keeping the demand in mind.

Author : National Research Council,Institute of Medicine,Board on Agriculture and Natural Resources,Food and Nutrition Board,Board on Life Sciences,Committee on Identifying and Assessing Unintended Effects of Genetically Engineered Foods on Human Health
Publisher : National Academies Press
Page : 254 pages
File Size : 42,5 Mb
Release : 2004-07-08
Category : Science
ISBN : 9780309166157

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Safety of Genetically Engineered Foods by National Research Council,Institute of Medicine,Board on Agriculture and Natural Resources,Food and Nutrition Board,Board on Life Sciences,Committee on Identifying and Assessing Unintended Effects of Genetically Engineered Foods on Human Health Pdf

Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Author : Matthew R. Willmann
Publisher : Burleigh Dodds Series in Agricultural Science
Page : 0 pages
File Size : 48,5 Mb
Release : 2021
Category : Electronic
ISBN : 1786764474

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Genome Editing for Precision Crop Breeding by Matthew R. Willmann Pdf

Part 1 of this volume reviews advances in gene editing techniques such as insertion-based genome edits, base editing, guide RNAs and CRISPR/Cas off targeting. Part 2 surveys applications of gene editing in key cereal and vegetable crops.

Author : Anonim
Publisher : Academic Press
Page : 264 pages
File Size : 42,9 Mb
Release : 2017-08-31
Category : Science
ISBN : 9780128117446

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Gene Editing in Plants by Anonim Pdf

Gene Editing in Plants, Volume 149 aims to provide the reader with an up-to-date survey of cutting-edge research with gene editing tools and an overview of the implications of this research on the nutritional quality of fruits, vegetables and grains. New chapters in the updated volume include topics relating to Genome Engineering and Agriculture: Opportunities and Challenges, the Use of CRISPR/Cas9 for Crop Improvement in Maize and Soybean, the Use of Zinc-Finger Nucleases for Crop Improvement, Gene Editing in Polyploid Crops: Wheat, Camelina, Canola, Potato, Cotton, Peanut, Sugar Cane, and Citrus, and Gene Editing With TALEN and CRISPR/Cas in Rice. This ongoing serial contain contributions from leading scientists and researchers in the field of gene editing in plants who describe the results of their own research in this rapidly expanding area of science. Shows the importance of revolutionary gene editing technology on plant biology research and its application to agricultural production Provides insight into what may lie ahead in this rapidly expanding area of plant research and development Contains contributions from major leaders in the field of plant gene editing

Author : Vijay Rani Rajpal,Deepmala Sehgal,Ravi Valluru,Sukhwinder Singh
Publisher : Frontiers Media SA
Page : 312 pages
File Size : 55,6 Mb
Release : 2023-07-10
Category : Science
ISBN : 9782832528112

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Current advances in genomics and gene editing tools for crop improvement in a changing climate scenario by Vijay Rani Rajpal,Deepmala Sehgal,Ravi Valluru,Sukhwinder Singh Pdf

Author : Om Prakash Gupta,Suhas Gorakh Karkute
Publisher : CRC Press
Page : 382 pages
File Size : 44,9 Mb
Release : 2021-07-25
Category : Science
ISBN : 9781000410198

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Genome Editing in Plants by Om Prakash Gupta,Suhas Gorakh Karkute Pdf

Genome Editing in Plants: Principles and Applications addresses the information of genome editing starting from principles and historical aspects to the latest advancements in the field. As genome-editing technology has emerged as promising and cutting edge, researchers around the world have started producing original research outputs, which have significantly improved our current understanding and potential of this technology. The initial chapters of this book describe different genome-editing tools as well as their principles and applications. Other chapters are dedicated to the present status and future applications of genome-editing techniques in various crop improvement programmes. Some of the advanced applications of CRISPR/Cas tools, such as base editing and RNA detection, along with regulatory aspects of genome-edited crops are described in detail. This book serves as a valuable resource to researchers in the field of crop improvement; graduate and postgraduate students engaged in plant molecular biology and biotechnology; academicians; and policy makers. Key Features: Addresses topics associated with historical development and principles of genome-editing technology Addresses basic mechanisms operating under each genome-editing technology Addresses its application in plants to design crops as per the current and future demands Addresses the regulatory mechanisms of genome-edited crops

Author : D.N. Bharadwaj
Publisher : CRC Press
Page : 700 pages
File Size : 40,8 Mb
Release : 2018-10-29
Category : Science
ISBN : 9781351358477

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Advanced Molecular Plant Breeding by D.N. Bharadwaj Pdf

This new volume provides a better understanding of molecular plant breeding in order to boost the quality of agriculture produce, to increase crop yields and to provide nutritious food for everyone by 2050. Scientists believe the challenge can be met by implementing new and improved techniques of quantitative trait inheritance in plant breeding. Integrating genomics and molecular biology into appropriate tools and methodologies can help to create genetically engineered plants, such as by using biotic and abiotic stress tolerance, molecular markers, ‘-omics’ technology, and genome editing.

Author : Takashi Yamamoto
Publisher : Springer
Page : 206 pages
File Size : 40,9 Mb
Release : 2015-01-05
Category : Science
ISBN : 9784431552277

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Targeted Genome Editing Using Site-Specific Nucleases by Takashi Yamamoto Pdf

This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.

Author : Rodolphe Barrangou,John van der Oost
Publisher : Springer Science & Business Media
Page : 300 pages
File Size : 45,8 Mb
Release : 2012-12-13
Category : Science
ISBN : 9783642346576

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CRISPR-Cas Systems by Rodolphe Barrangou,John van der Oost Pdf

CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.

Author : Kashmir Singh
Publisher : Springer Nature
Page : 404 pages
File Size : 45,6 Mb
Release : 2024-06-28
Category : Electronic
ISBN : 9789819988747

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Biotechnological Advances for Disease Tolerance in Plants by Kashmir Singh Pdf