Targeted Genome Editing Using Site Specific Nucleases

Author : Takashi Yamamoto
Publisher : Springer
Page : 206 pages
File Size : 41,8 Mb
Release : 2015-01-05
Category : Science
ISBN : 9784431552277

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Targeted Genome Editing Using Site-Specific Nucleases by Takashi Yamamoto Pdf

This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.

Author : Anonim
Publisher : Academic Press
Page : 594 pages
File Size : 44,6 Mb
Release : 2014-11-04
Category : Science
ISBN : 9780128013342

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The Use of CRISPR/cas9, ZFNs, TALENs in Generating Site-Specific Genome Alterations by Anonim Pdf

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease

Author : Toni Cathomen,Matthew Hirsch,Matthew Porteus
Publisher : Springer
Page : 263 pages
File Size : 50,7 Mb
Release : 2016-03-04
Category : Medical
ISBN : 9781493935093

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Genome Editing by Toni Cathomen,Matthew Hirsch,Matthew Porteus Pdf

This comprehensive volume explores human genetic engineering its pre-clinical and clinical applications, current developments, and as treatment for hereditary diseases. It presents and evaluates the most recent advances in the understanding of mammalian host DNA repair mechanisms, such as double-strand break induced gene targeting and mutagenesis, the development of zinc-finger nucleases, genome editing for neuromuscular diseases, phase integrases, triplex forming oligonucleotides and peptide nucleic acids, aptamer-guided gene targeting, AAV gene editing via DSB repair, engineered nucleases and trinucleotide repeat diseases, and creation of HIV-resistant cells. The expertly authored chapters contextualize current developments within the history of genome editing while also discussing the current and potential safety concerns of this rapidly growing field. Genome Editing: The Next Step in Gene Therapy, the latest volume in the American Society of Gene and Cell Therapy series, deftly illuminates the potential of genetic engineering technology to eradicate today’s deadliest and most prolific diseases. It is ideal reading for clinicians and researchers in genetics and immunology.

Author : Kursad Turksen
Publisher : Springer
Page : 166 pages
File Size : 41,6 Mb
Release : 2016-08-09
Category : Science
ISBN : 9783319341484

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Genome Editing by Kursad Turksen Pdf

This timely volume explores the use of CRISPR-Cas9 for genome editing, presenting cutting-edge techniques and their applications in treatment of disease. The chapters describe latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular disease, diabetes mellitus, retinitis pigmentosa, and others. The final chapters round out the book with a discussion of controversies and future directions. Genome Editing is an essential, of-the-moment contribution to this rapidly growing field. Drawing from a wealth of international perspectives, it presents novel techniques and applications for the engineering of the human genome. This book is essential reading for all clinicians and researchers in stem cells, regenerative medicine, genomics, biochemical and biomedical engineering- especially those interested in learning more about genome editing and applying it in a targeted, specific way.

Author : Ayal Hendel,Rasmus O. Bak
Publisher : Frontiers Media SA
Page : 157 pages
File Size : 46,7 Mb
Release : 2023-05-16
Category : Science
ISBN : 9782832523452

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Therapeutic Gene Correction Strategies Based on CRISPR Systems or Other Engineered Site-specific Nucleases by Ayal Hendel,Rasmus O. Bak Pdf

Author : Feng Zhang,Holger Puchta,James G. Thomson
Publisher : Springer
Page : 166 pages
File Size : 44,8 Mb
Release : 2015-04-21
Category : Science
ISBN : 9781493925568

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Advances in New Technology for Targeted Modification of Plant Genomes by Feng Zhang,Holger Puchta,James G. Thomson Pdf

Over the past 50 years, biotechnology has been the major driving force for increasing crop productivity. Particularly, advances in plant genetic engineering technologies have opened up vast new opportunities for plant researchers and breeders to create new crop varieties with desirable traits. Recent development of precise genome modification methods, such as targeted gene knock-out/knock-in and precise gene replacement, moves genetic engineering to another level and offers even more potentials for improving crop production. The work provides an overview of the latest advances on precise genomic engineering technologies in plants. Topics include recombinase and engineered nucleases-mediated targeted modification, negative/positive selection-based homologous recombination and oligo nucleotide-mediated recombination. Finally, challenges and impacts of the new technologies on present regulations for genetic modification organisms (GMOs) will be discussed.

Author : Michael Kormann
Publisher : BoD – Books on Demand
Page : 228 pages
File Size : 41,6 Mb
Release : 2016-05-18
Category : Science
ISBN : 9789535123927

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Modern Tools for Genetic Engineering by Michael Kormann Pdf

Site-specific endonucleases create double-strand breaks within the genome and can be targeted to literally any genetic mutation. Together with a repair template, a correction of the defective locus becomes possible. This book offers insight into the modern tools of genome editing, their hurdles and their huge potential. A new era of in vivo genetic engineering has begun.

Author : National Academies of Sciences, Engineering, and Medicine,National Academy of Medicine,National Academy of Sciences,Committee on Human Gene Editing: Scientific, Medical, and Ethical Considerations
Publisher : National Academies Press
Page : 329 pages
File Size : 49,8 Mb
Release : 2017-08-13
Category : Medical
ISBN : 9780309452885

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Human Genome Editing by National Academies of Sciences, Engineering, and Medicine,National Academy of Medicine,National Academy of Sciences,Committee on Human Gene Editing: Scientific, Medical, and Ethical Considerations Pdf

Genome editing is a powerful new tool for making precise alterations to an organism's genetic material. Recent scientific advances have made genome editing more efficient, precise, and flexible than ever before. These advances have spurred an explosion of interest from around the globe in the possible ways in which genome editing can improve human health. The speed at which these technologies are being developed and applied has led many policymakers and stakeholders to express concern about whether appropriate systems are in place to govern these technologies and how and when the public should be engaged in these decisions. Human Genome Editing considers important questions about the human application of genome editing including: balancing potential benefits with unintended risks, governing the use of genome editing, incorporating societal values into clinical applications and policy decisions, and respecting the inevitable differences across nations and cultures that will shape how and whether to use these new technologies. This report proposes criteria for heritable germline editing, provides conclusions on the crucial need for public education and engagement, and presents 7 general principles for the governance of human genome editing.

Author : Rodolphe Barrangou,John van der Oost
Publisher : Springer Science & Business Media
Page : 300 pages
File Size : 44,9 Mb
Release : 2012-12-13
Category : Science
ISBN : 9783642346576

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CRISPR-Cas Systems by Rodolphe Barrangou,John van der Oost Pdf

CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.

Author : Irwin M. Arias,Harvey J. Alter,James L. Boyer,David E. Cohen,David A. Shafritz,Snorri S. Thorgeirsson,Allan W. Wolkoff
Publisher : John Wiley & Sons
Page : 1156 pages
File Size : 51,5 Mb
Release : 2020-03-09
Category : Medical
ISBN : 9781119436829

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The Liver by Irwin M. Arias,Harvey J. Alter,James L. Boyer,David E. Cohen,David A. Shafritz,Snorri S. Thorgeirsson,Allan W. Wolkoff Pdf

Bridging the gap between basic scientific advances and the understanding of liver disease — the extensively revised new edition of the premier text in the field. The latest edition of The Liver: Biology and Pathobiology remains a definitive volume in the field of hepatology, relating advances in biomedical sciences and engineering to understanding of liver structure, function, and disease pathology and treatment. Contributions from leading researchers examine the cell biology of the liver, the pathobiology of liver disease, the liver’s growth, regeneration, metabolic functions, and more. Now in its sixth edition, this classic text has been exhaustively revised to reflect new discoveries in biology and their influence on diagnosing, managing, and preventing liver disease. Seventy new chapters — including substantial original sections on liver cancer and groundbreaking advances that will have significant impact on hepatology — provide comprehensive, fully up-to-date coverage of both the current state and future direction of hepatology. Topics include liver RNA structure and function, gene editing, single-cell and single-molecule genomic analyses, the molecular biology of hepatitis, drug interactions and engineered drug design, and liver disease mechanisms and therapies. Edited by globally-recognized experts in the field, this authoritative volume: Relates molecular physiology to understanding disease pathology and treatment Links the science and pathology of the liver to practical clinical applications Features 16 new “Horizons” chapters that explore new and emerging science and technology Includes plentiful full-color illustrations and figures The Liver: Biology and Pathobiology, Sixth Edition is an indispensable resource for practicing and trainee hepatologists, gastroenterologists, hepatobiliary and liver transplant surgeons, and researchers and scientists in areas including hepatology, cell and molecular biology, virology, and drug metabolism.

Author : Shabir Hussain Wani,Goetz Hensel
Publisher : Springer Nature
Page : 338 pages
File Size : 45,6 Mb
Release : 2022-11-08
Category : Science
ISBN : 9783031080722

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Genome Editing by Shabir Hussain Wani,Goetz Hensel Pdf

Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : Yuan-Chuan Chen,Shiu-Jau Chen
Publisher : BoD – Books on Demand
Page : 110 pages
File Size : 43,9 Mb
Release : 2019-05-29
Category : Medical
ISBN : 9781789845082

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Gene Editing by Yuan-Chuan Chen,Shiu-Jau Chen Pdf

Gene-editing technologies (e.g., ZFNs, TALENs, and CRISPRs/Cas9) have been extensively used as tools in basic research. They are further applied in manufacturing agricultural products, food, industrial products, medicinal products, etc. Particularly, the discovery of medicinal products using gene-editing technologies will open a new era for human therapeutics. Though there are still many technical and ethical challenges ahead of us, more and more products based on gene-editing technologies have been approved for marketing. These technologies are promising for multiple applications. Their development and implications should be explored in the broadest context possible. Future research directions should also be highlighted. In this book, the applications, perspectives, and challenges of gene-editing technologies are significantly demonstrated and discussed.

Author : Anonim
Publisher : Academic Press
Page : 264 pages
File Size : 40,9 Mb
Release : 2017-08-31
Category : Science
ISBN : 9780128117446

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Gene Editing in Plants by Anonim Pdf

Gene Editing in Plants, Volume 149 aims to provide the reader with an up-to-date survey of cutting-edge research with gene editing tools and an overview of the implications of this research on the nutritional quality of fruits, vegetables and grains. New chapters in the updated volume include topics relating to Genome Engineering and Agriculture: Opportunities and Challenges, the Use of CRISPR/Cas9 for Crop Improvement in Maize and Soybean, the Use of Zinc-Finger Nucleases for Crop Improvement, Gene Editing in Polyploid Crops: Wheat, Camelina, Canola, Potato, Cotton, Peanut, Sugar Cane, and Citrus, and Gene Editing With TALEN and CRISPR/Cas in Rice. This ongoing serial contain contributions from leading scientists and researchers in the field of gene editing in plants who describe the results of their own research in this rapidly expanding area of science. Shows the importance of revolutionary gene editing technology on plant biology research and its application to agricultural production Provides insight into what may lie ahead in this rapidly expanding area of plant research and development Contains contributions from major leaders in the field of plant gene editing

Author : Thorben Sprink,Ralf Alexander Wilhelm,Armin Spök,Jürgen Robienski,Stephan Schleissing,Joachim Hermann Schiemann
Publisher : Frontiers Media SA
Page : 252 pages
File Size : 44,6 Mb
Release : 2020-04-22
Category : Electronic
ISBN : 9782889636709

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Plant Genome Editing – Policies and Governance by Thorben Sprink,Ralf Alexander Wilhelm,Armin Spök,Jürgen Robienski,Stephan Schleissing,Joachim Hermann Schiemann Pdf

Author : The Royal Society,National Academy of Sciences,National Academy of Medicine,International Commission on the Clinical Use of Human Germline Genome Editing
Publisher : National Academies Press
Page : 239 pages
File Size : 45,8 Mb
Release : 2021-01-16
Category : Medical
ISBN : 9780309671132

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Heritable Human Genome Editing by The Royal Society,National Academy of Sciences,National Academy of Medicine,International Commission on the Clinical Use of Human Germline Genome Editing Pdf

Heritable human genome editing - making changes to the genetic material of eggs, sperm, or any cells that lead to their development, including the cells of early embryos, and establishing a pregnancy - raises not only scientific and medical considerations but also a host of ethical, moral, and societal issues. Human embryos whose genomes have been edited should not be used to create a pregnancy until it is established that precise genomic changes can be made reliably and without introducing undesired changes - criteria that have not yet been met, says Heritable Human Genome Editing. From an international commission of the U.S. National Academy of Medicine, U.S. National Academy of Sciences, and the U.K.'s Royal Society, the report considers potential benefits, harms, and uncertainties associated with genome editing technologies and defines a translational pathway from rigorous preclinical research to initial clinical uses, should a country decide to permit such uses. The report specifies stringent preclinical and clinical requirements for establishing safety and efficacy, and for undertaking long-term monitoring of outcomes. Extensive national and international dialogue is needed before any country decides whether to permit clinical use of this technology, according to the report, which identifies essential elements of national and international scientific governance and oversight.