Advancing Development Of Synthetic Gene Regulators

Author : Anandhakumar Chandran
Publisher : Springer
Page : 114 pages
File Size : 47,6 Mb
Release : 2017-09-19
Category : Science
ISBN : 9789811065477

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Advancing Development of Synthetic Gene Regulators by Anandhakumar Chandran Pdf

This book focuses on an “outside the box” notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)–N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence.

Author : Institute of Medicine,Board on Global Health,Forum on Microbial Threats
Publisher : National Academies Press
Page : 570 pages
File Size : 46,9 Mb
Release : 2011-12-30
Category : Science
ISBN : 9780309219396

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The Science and Applications of Synthetic and Systems Biology by Institute of Medicine,Board on Global Health,Forum on Microbial Threats Pdf

Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.

Author : Vijai Singh
Publisher : Springer Nature
Page : 349 pages
File Size : 48,5 Mb
Release : 2020-04-13
Category : Science
ISBN : 9789811500817

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Advances in Synthetic Biology by Vijai Singh Pdf

This book addresses the design of emerging conceptual tools, technologies and systems including novel synthetic parts, devices, circuits, oscillators, biological gates, and small regulatory RNAs (riboregulators and riboswitches), which serve as versatile control elements for regulating gene expression. Synthetic biology, a rapidly growing field that involves the application of engineering principles in biology, is now being used to develop novel systems for a wide range of applications including diagnostics, cell reprogramming, therapeutics, enzymes, vaccines, biomaterials, biofuels, fine chemicals and many more. The book subsequently summarizes recent developments in technologies for assembling synthetic genomes, minimal genomes, synthetic biology toolboxes, CRISPR-Cas systems, cell-free protein synthesis systems and microfluidics. Accordingly, it offers a valuable resource not only for beginners in synthetic biology, but also for researchers, students, scientists, clinicians, stakeholders and policymakers interested in the potential held by synthetic biology.

Author : National Research Council,National Academy of Engineering,Division on Earth and Life Studies,Board on Life Sciences,Policy and Global Affairs,Committee on Science, Technology, and Law
Publisher : National Academies Press
Page : 80 pages
File Size : 43,9 Mb
Release : 2013-08-05
Category : Science
ISBN : 9780309225861

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Positioning Synthetic Biology to Meet the Challenges of the 21st Century by National Research Council,National Academy of Engineering,Division on Earth and Life Studies,Board on Life Sciences,Policy and Global Affairs,Committee on Science, Technology, and Law Pdf

Synthetic biology -- unlike any research discipline that precedes it -- has the potential to bypass the less predictable process of evolution to usher in a new and dynamic way of working with living systems. Ultimately, synthetic biologists hope to design and build engineered biological systems with capabilities that do not exist in natural systems -- capabilities that may ultimately be used for applications in manufacturing, food production, and global health. Importantly, synthetic biology represents an area of science and engineering that raises technical, ethical, regulatory, security, biosafety, intellectual property, and other issues that will be resolved differently in different parts of the world. As a better understanding of the global synthetic biology landscape could lead to tremendous benefits, six academies -- the United Kingdom's Royal Society and Royal Academy of Engineering, the United States' National Academy of Sciences and National Academy of Engineering, and the Chinese Academy of Science and Chinese Academy of Engineering -- organized a series of international symposia on the scientific, technical, and policy issues associated with synthetic biology. Positioning Synthetic Biology to Meet the Challenges of the 21st Century summarizes the symposia proceedings.

Author : National Academies of Sciences, Engineering, and Medicine,Division on Earth and Life Studies,Board on Life Sciences,Board on Chemical Sciences and Technology,Committee on Strategies for Identifying and Addressing Potential Biodefense Vulnerabilities Posed by Synthetic Biology
Publisher : National Academies Press
Page : 189 pages
File Size : 46,5 Mb
Release : 2019-01-05
Category : Technology & Engineering
ISBN : 9780309465182

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Biodefense in the Age of Synthetic Biology by National Academies of Sciences, Engineering, and Medicine,Division on Earth and Life Studies,Board on Life Sciences,Board on Chemical Sciences and Technology,Committee on Strategies for Identifying and Addressing Potential Biodefense Vulnerabilities Posed by Synthetic Biology Pdf

Scientific advances over the past several decades have accelerated the ability to engineer existing organisms and to potentially create novel ones not found in nature. Synthetic biology, which collectively refers to concepts, approaches, and tools that enable the modification or creation of biological organisms, is being pursued overwhelmingly for beneficial purposes ranging from reducing the burden of disease to improving agricultural yields to remediating pollution. Although the contributions synthetic biology can make in these and other areas hold great promise, it is also possible to imagine malicious uses that could threaten U.S. citizens and military personnel. Making informed decisions about how to address such concerns requires a realistic assessment of the capabilities that could be misused. Biodefense in the Age of Synthetic Biology explores and envisions potential misuses of synthetic biology. This report develops a framework to guide an assessment of the security concerns related to advances in synthetic biology, assesses the levels of concern warranted for such advances, and identifies options that could help mitigate those concerns.

Author : Edenilson Brandl
Publisher : Edenilson Brandl
Page : 515 pages
File Size : 49,7 Mb
Release : 2024-06-17
Category : Health & Fitness
ISBN : 8210379456XXX

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Synthetic Biology, Genetics and Advanced Topics by Edenilson Brandl Pdf

Synthetic Biology is a scientific discipline that has the potential to revolutionize the way we understand and manipulate life. Combining knowledge from biology, genetics, engineering and computing, Synthetic Biology opens up new possibilities for creating personalized biological systems, with applications ranging from regenerative medicine to the production of biofuels. In this book, we will explore the principles, techniques, and applications of synthetic biology, with the aim of providing a comprehensive and up-to-date overview of this ever-evolving field. Throughout the chapters, we will delve into the molecular foundations of Synthetic Biology, exploring the biological components and processes involved in building synthetic biological systems. We will present several applications of Synthetic Biology in different sectors, highlighting its role in the pharmaceutical industry, in the production of biofuels, in the development of personalized vaccines and in regenerative medicine. We will see how the manipulation of synthetic stem cells can revolutionize the area of tissue regeneration and how metabolic engineering allows us to design microorganisms capable of producing valuable compounds. Bioinformatics plays a crucial role in Synthetic Biology, and we will dedicate a chapter to exploring how the analysis of genomic data and the use of bioinformatics algorithms contribute to the advancement of this discipline. In addition, we will cover topics such as genome editing using CRISPR technology, synthetic protein engineering, and directed evolution in the laboratory. With the advancement of technology, it has become possible to manufacture living tissue in the laboratory, and we will discuss biofabrication and 3D printing of living tissue, as well as the use of microfluidics in the manipulation and analysis of synthetic biological systems on a microscopic scale. We will also address ethical and biosafety issues related to Synthetic Biology, recognizing the importance of a responsible approach in all its applications. This book is intended for students, researchers, and practitioners interested in exploring the frontiers of synthetic biology. We hope it will be an inspiring source of knowledge, awakening the curiosity and innovative spirit of all readers. We invite you to embark on this exciting journey through Synthetic Biology, in search of a deeper understanding of life and its transformative possibilities.

Author : National Academies of Sciences, Engineering, and Medicine,Division on Earth and Life Studies,Board on Chemical Sciences and Technology,Board on Agriculture and Natural Resources,Board on Life Sciences,Committee on Future Biotechnology Products and Opportunities to Enhance Capabilities of the Biotechnology Regulatory System
Publisher : National Academies Press
Page : 231 pages
File Size : 51,6 Mb
Release : 2017-07-28
Category : Science
ISBN : 9780309452052

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Preparing for Future Products of Biotechnology by National Academies of Sciences, Engineering, and Medicine,Division on Earth and Life Studies,Board on Chemical Sciences and Technology,Board on Agriculture and Natural Resources,Board on Life Sciences,Committee on Future Biotechnology Products and Opportunities to Enhance Capabilities of the Biotechnology Regulatory System Pdf

Between 1973 and 2016, the ways to manipulate DNA to endow new characteristics in an organism (that is, biotechnology) have advanced, enabling the development of products that were not previously possible. What will the likely future products of biotechnology be over the next 5â€"10 years? What scientific capabilities, tools, and/or expertise may be needed by the regulatory agencies to ensure they make efficient and sound evaluations of the likely future products of biotechnology? Preparing for Future Products of Biotechnology analyzes the future landscape of biotechnology products and seeks to inform forthcoming policy making. This report identifies potential new risks and frameworks for risk assessment and areas in which the risks or lack of risks relating to the products of biotechnology are well understood.

Author : Wilfried Weber,Martin Fussenegger
Publisher : Humana Press
Page : 393 pages
File Size : 46,9 Mb
Release : 2011-11-17
Category : Science
ISBN : 1617794112

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Synthetic Gene Networks by Wilfried Weber,Martin Fussenegger Pdf

The rapid expansion of synthetic biology is due to the design and construction of synthetic gene networks that have opened many new avenues in fundamental and applied research. Synthetic Gene Networks: Methods and Protocols provides the necessary information to design and construct synthetic gene networks in different host backgrounds. Divided into four convenient sections, this volume focuses on design concepts to devise synthetic gene networks and how mathematical models can be applied to the predictable engineering of desired network features. The volume continues by highlighting the construction and validation of biologic tools, describing strategies to optimize and streamline the host cell for optimized network performance, and covering how optimally designed gene networks can be implemented in a large variety of host cells ranging from bacteria over yeast and insect cells to plant and mammalian cell culture. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Synthetic Gene Networks: Methods and Protocols serves as an invaluable resource for established biologists, engineers, and computer scientists or novices just entering into the rapidly growing field of synthetic biology

Author : Maria Cristina Galli
Publisher : Springer Nature
Page : 243 pages
File Size : 51,5 Mb
Release : 2023-08-01
Category : Science
ISBN : 9783031345678

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Regulatory Aspects of Gene Therapy and Cell Therapy Products by Maria Cristina Galli Pdf

This book discusses the different regulatory pathways for Advanced Therapy Medicinal Products implemented by national agencies in North and South America, Europe and Asia and by international bodies in the effort of international harmonization. This book represents an update of the first edition, as it covers regulatory novelties and accumulated experience in the regions already addressed. In addition, this new edition offers a wider international perspective: new chapters are included covering Advanced Therapy Medicinal Products regulations in India, Malaysia, Spain and Thailand, the European Pharmacopoeia texts for gene therapy medicinal products as well as international harmonization programs. Each chapter, authored by experts from various regulatory bodies throughout the international community, walks the reader through the applications of nonclinical research to translational clinical research to licensure and therapeutic use of these innovative products. More specifically, each chapter offers insights into fundamental considerations that are essential for developers of Advanced Therapy Medicinal Products in the areas of product quality, pharmacology and toxicology, clinical trial design and HTA pathways, as well as pertinent ‘must-know’ guidelines and regulations. Regulatory Aspects of Gene Therapy and Cell Therapy Products: a Global Perspective is part of the American Society of Gene & Cell Therapy sub-series of the highly successful Advances in Experimental Medicine and Biology series. It is essential reading for graduate students, clinicians, and researchers interested in gene and cell therapy and the regulation of pharmaceuticals.

Author : National Academies of Sciences, Engineering, and Medicine,Health and Medicine Division,Board on Health Sciences Policy,Forum on Neuroscience and Nervous System Disorders
Publisher : National Academies Press
Page : 93 pages
File Size : 43,8 Mb
Release : 2019-12-01
Category : Medical
ISBN : 9780309495844

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Advancing Gene-Targeted Therapies for Central Nervous System Disorders by National Academies of Sciences, Engineering, and Medicine,Health and Medicine Division,Board on Health Sciences Policy,Forum on Neuroscience and Nervous System Disorders Pdf

On April 23 and 24, 2019 the Forum on Neuroscience and Nervous System Disorders convened a workshop titled "Advancing Gene-Targeted Therapies for Central Nervous System Disorders" in Washington, DC. This public workshop brought together experts and key stakeholders from academia, government, industry, philanthropic foundations, and disease/patient-focused nonprofit organizations to explore approaches for advancing the development of gene-targeted therapies for central nervous system (CNS) disorders, and implications of developing these therapies. Participants explored lessons learned from both successful and unsuccessful clinical development programs; new knowledge about the genetic underpinnings of brain disorders; the current status and future potential of gene-targeted therapies for CNS disorders; challenges and potential solutions for translating preclinical findings to approved therapies; and patient and caregiver perspectives. They also discussed what will be needed to develop these therapies for common disorders such as Alzheimer's and Parkinson's disease, as well as neuropsychiatric and neurodevelopmental disorders such as schizophrenia and autism. The workshop included approaches that target both DNA and RNA, as well as gene products using viral vectors, antisense oligonucleotides, and RNA interference. This publication summarizes the presentations and discussion of the workshop.

Author : Mohit Kumar Jolly,Jean-Pascal Capp,Ankur Sharma
Publisher : Frontiers Media SA
Page : 130 pages
File Size : 40,5 Mb
Release : 2021-11-01
Category : Science
ISBN : 9782889714216

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Non-Genetic Heterogeneity in Development and Disease by Mohit Kumar Jolly,Jean-Pascal Capp,Ankur Sharma Pdf

Author : Byong H. Lee
Publisher : John Wiley & Sons
Page : 500 pages
File Size : 40,7 Mb
Release : 2021-10-25
Category : Technology & Engineering
ISBN : 9781119042778

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Advanced Fermentation and Cell Technology by Byong H. Lee Pdf

ADVANCED FERMENTATION AND CELL TECHNOLOGY A comprehensive and up-to-date reference covering both conventional and novel industrial fermentation technologies and their applications Fermentation and cell culture technologies encompass more than the conventional microbial and enzyme systems used in the agri-food, biochemical, bioenergy and pharmaceutical industries. New technologies such as genetic engineering, systems biology, protein engineering, and mammalian cell and plant cell systems are expanding rapidly, as is the demand for sustainable production of bioingredients, drugs, bioenergy and biomaterials. As the growing biobased economy drives innovation, industrial practitioners, instructors, researchers, and students must keep pace with the development and application of novel fermentation processes and a variety of cell technologies. Advanced Fermentation and Cell Technology provides a balanced and comprehensive overview of the microbial, mammalian, and plant cell technologies used by the modern biochemical process industry to develop new and improved processes and products. This authoritative volume covers the essential features of advanced fermentation and cell technology, and highlights the interaction of food fermentation and cell culture biopharmaceutical actives. Detailed chapters, organized into five sections, cover microbial cell technology, animal and plant cell technology, safety issues of new biotechnologies, and applications of microbial fermentation to food products, chemicals, and pharmaceuticals. Written by an internationally-recognized expert in food biotechnology, this comprehensive volume: Covers both conventional and novel industrial fermentation technologies and their applications in a range of industries Discusses current progress in novel fermentation, cell culture, commercial recombinant bioproducts technologies Includes overviews of the global market size of bioproducts and the fundamentals of cell technology Highlights the importance of sustainability, Good Manufacturing Practices (GMP), quality assurance, and regulatory practices Explores microbial cell technology and culture tools and techniques such as genome shuffling and recombinant DNA technology, RNA interference and CRISPR technology, molecular thermodynamics, protein engineering, proteomics and bioinformatics, and synthetic biology Advanced Fermentation and Cell Technology is an ideal resource for students of food science, biotechnology, microbiology, agricultural sciences, biochemical engineering, and biochemistry, and is a valuable reference for food scientists, researchers, and technologists throughout the food industry, particularly the dairy, bakery, and fermented beverage sectors.

Author : Miguel Fernández-Niño,Luis H. Reyes
Publisher : BoD – Books on Demand
Page : 106 pages
File Size : 50,5 Mb
Release : 2022-02-02
Category : Science
ISBN : 9781839696381

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Synthetic Genomics by Miguel Fernández-Niño,Luis H. Reyes Pdf

The current advances in sequencing, data mining, DNA synthesis, cloning, in silico modeling, and genome editing have opened a new field of research known as Synthetic Genomics. The main goal of this emerging area is to engineer entire synthetic genomes from scratch using pre-designed building blocks obtained by chemical synthesis and rational design. This has opened the possibility to further improve our understanding of genome fundamentals by considering the effect of the whole biological system on biological function. Moreover, the construction of non-natural biological systems has allowed us to explore novel biological functions so far not discovered in nature. This book summarizes the current state of Synthetic Genomics, providing relevant examples in this emerging field.

Author : Datta Madamwar,Kunal R. Jain,Chirayu Desai,Eric D. van Hullebusch
Publisher : Frontiers Media SA
Page : 214 pages
File Size : 52,8 Mb
Release : 2021-11-26
Category : Science
ISBN : 9782889716982

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Advanced Bioremediation Technologies and Processes for the Treatment of Synthetic Organic Compounds (SOCs) by Datta Madamwar,Kunal R. Jain,Chirayu Desai,Eric D. van Hullebusch Pdf

Dr. Datta Madamwar holds a provisional patent related to the theme of this Research Topic. All other Topic Editors declare no competing interests with regards to the Research Topic subject.

Author : Aryadeep Roychoudhury
Publisher : CRC Press
Page : 549 pages
File Size : 40,8 Mb
Release : 2023-12-22
Category : Medical
ISBN : 9781003822127

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Advancement of Melatonin Research in Plants by Aryadeep Roychoudhury Pdf

Melatonin (N-acetyl-5-methoxytryptamine) is a widely studied biomolecule, and its function has been investigated in bacteria, mammals, birds, amphibians, reptiles, fish and plants. Melatonin research in plants the last five years has attained an exponential growth phase and the number of publications related to phytomelatonin has significantly increased in present times. All this research is directed towards establishing multifarious roles of melatonin in plants, including the promotion of seed germination and seedling growth, influencing plant development and senescence, alteration of flowering and fruiting time and grain yield, modulating circadian rhythms, improving resistance against biotic stress as well as tolerance to abiotic stress, reducing oxidative stress, and stimulating plant immune response. However, to date, an exhaustive book, solely and exclusively dedicated to melatonin research on various aspects of plant biology, has not been published by any renowned publisher or is available in the market. Keeping all these facts in mind, this book aims to compile the most recent developments in the arena of melatonin-mediated regulation of plant physiology under optimal and sub-optimal conditions. This book will initiate and introduce the readers to the state-of-the-art developments and trends in this field of study and will largely cater to the scientific need of scientists engaged in melatonin research across the globe. The book will be unique of its kind and will draw the attention of scientific and research communities engaged in the field of melatonin research. The key features of this book: Authors will address the topics which would provide advances in the understanding of diverse functions of melatonin in plants, encompassing almost all the aspects that have been investigated so far Integrate the overall mechanistic function of melatonin, genomics and proteomics tools, and genetic engineering to manipulate endogenous melatonin levels, thus giving a holistic view of plant-level studies under normal or challenging situations Largely help the readers to design future research programs in exploiting melatonin as a protective agent to develop climate-resilient and pathogen-resistant plants Benefit the crop protection program and ensure food security Chapters will be supplemented with ample illustrations and figures This book will be beneficial to research scholars and Ph.D. students involved in melatonin research and working on stress physiology, stress proteins, genomics, proteomics and genetic engineering in different universities and research institutes all over the world. The book can also be used as an advanced reference book for the coursework of research and master’s level students. It will be of use to people involved in improving plant growth and yield, as well as the performance of plant species in the face of climate change and pathogen infection, using melatonin as the prime protective agent and signaling molecule. Although the book will directly benefit the academic and scientific community, it will also indirectly draw the attention of industries or companies that manufacture or sell chemical products, since the book will highlight in detail the antioxidative potential of melatonin. This will create impetus at the industrial level for the isolation and synthesis of melatonin as a beneficial chemical agent. Applying melatonin as an ameliorating agent against different stresses at the field level will also increase the demand for this chemical among the farmers and will promote large-scale sales and utilization of melatonin for ensuring crop protection and increasing yield.