Agrobacterium Biology And Its Application To Transgenic Plant Production

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Agrobacterium biology and its application to transgenic plant production

Hau-Hsuan Hwang,Stanton B. Gelvin
Agrobacterium biology and its application to transgenic plant production Book PDF

Author : Hau-Hsuan Hwang,Stanton B. Gelvin
Publisher : Frontiers Media SA
Page : 167 pages
File Size : 41,9 Mb
Release : 2015-06-26
Category : Botany
ISBN : 9782889195749

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Agrobacterium biology and its application to transgenic plant production by Hau-Hsuan Hwang,Stanton B. Gelvin Pdf

The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.

Agrobacterium: From Biology to Biotechnology

Tzvi Tzfira,Vitaly Citovsky
Agrobacterium From Biology to Biotechnology Book PDF

Author : Tzvi Tzfira,Vitaly Citovsky
Publisher : Springer Science & Business Media
Page : 768 pages
File Size : 46,9 Mb
Release : 2007-12-25
Category : Science
ISBN : 9780387722900

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Agrobacterium: From Biology to Biotechnology by Tzvi Tzfira,Vitaly Citovsky Pdf

Agrobacterium is a plant pathogen which causes the “crown-gall” disease, a neoplastic growth that results from the transfer of a well-defined DNA segment (“transferred DNA”, or “T-DNA”) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.

Transgenic Plants

Leandro Peña
Transgenic Plants Book PDF

Author : Leandro Peña
Publisher : Springer Science & Business Media
Page : 427 pages
File Size : 48,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592598274

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Transgenic Plants by Leandro Peña Pdf

The aim of Transgenic Plants: Methods and Protocols is to provide a source of information to guide the reader through a wide range of frequently used, broadly applicable, and easily reproducible techniques involved in the gene- tion of transgenic plants. Its step-by-step approach covers a series of methods for genetically transforming plant cells and tissues, and for recovering whole transgenic plants from them. The volume then moves on to the use of sele- able and reporter markers, positive selection, marker elimination after rec- ery of transgenic plants, and the analysis of transgene integration, expression, and localization in the plant genome. Although contributors usually refer to model plants in most chapters, the protocols described herein should be widely applicable to many plant species. The last two sections are devoted to me- ods of risk assessment and to exploring the current and future applications of transgenic technology in agriculture and its social implications in a case study. Transgenic Plants: Methods and Protocols is divided into six major s- tions plus an introduction, comprising 27 chapters. Part I, the Introduction, is a review of the past, present, and perspectives of the transgenic plants, from the discovery of Agrobacterium tumefaciens as a feasible transformation vector, to its use as a tool to study gene expression and function, and the current and possible future applications of this technology in agriculture, industry, and medicine.

Transgenic Crops of the World

Ian S. Curtis
Transgenic Crops of the World Book PDF

Author : Ian S. Curtis
Publisher : Springer Science & Business Media
Page : 448 pages
File Size : 48,6 Mb
Release : 2012-08-10
Category : Technology & Engineering
ISBN : 9781402023330

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Transgenic Crops of the World by Ian S. Curtis Pdf

Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants.

Plant Biotechnology and Genetics

C. Neal Stewart, Jr.
Plant Biotechnology and Genetics Book PDF

Author : C. Neal Stewart, Jr.
Publisher : John Wiley & Sons
Page : 554 pages
File Size : 53,6 Mb
Release : 2012-12-13
Category : Science
ISBN : 9781118589489

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Plant Biotechnology and Genetics by C. Neal Stewart, Jr. Pdf

Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.

Advances in Plant Transgenics: Methods and Applications

Ramalingam Sathishkumar,Sarma Rajeev Kumar,Jagadeesan Hema,Venkidasamy Baskar
Advances in Plant Transgenics Methods and Applications Book PDF

Author : Ramalingam Sathishkumar,Sarma Rajeev Kumar,Jagadeesan Hema,Venkidasamy Baskar
Publisher : Springer Nature
Page : 374 pages
File Size : 54,7 Mb
Release : 2019-11-15
Category : Science
ISBN : 9789811396243

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Advances in Plant Transgenics: Methods and Applications by Ramalingam Sathishkumar,Sarma Rajeev Kumar,Jagadeesan Hema,Venkidasamy Baskar Pdf

The green revolution led to the development of improved varieties of crops, especially cereals, and since then, classical or molecular breeding has resulted in the creation of economically valuable species. Thanks to recent developments in genetic engineering, it has become possible to introduce genes from different sources, such as bacteria, fungi, viruses, mice and humans, to plants. This technology has made the scientific community aware of the critical role of transgenics, not only as a means of producing stress tolerant crops but also as a platform for the production of therapeutics through molecular farming. This book discusses the commercial applications of plant transgenic technologies, including the use of transgenic cell culture approachesto improve the production of metabolites and high-value therapeutics as well as transgenic plants in pest management. It also explores generation of novel vectors, protein production using chloroplast engineering and the latest developments in this area, such as genome editing in plants. Featuring general discussions and research papers by leading international experts, it is a valuable resource for scientists, teachers, students and industrialists working in the field.

Genetic Transformation

María Alvarez
Genetic Transformation Book PDF

Author : María Alvarez
Publisher : BoD – Books on Demand
Page : 344 pages
File Size : 45,7 Mb
Release : 2011-09-06
Category : Medical
ISBN : 9789533073644

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Genetic Transformation by María Alvarez Pdf

Genetic transformation of plants has revolutionized both basic and applied plant research. Plant molecular biology and physiology benefit from this power fool, as well as biotechnology. This book is a review of some of the most significant achievements that plant transformation has brought to the fields of Agrobacterium biology, crop improvement and, flower, fruit and tree amelioration. Also, it examines their impact on molecular farming, phytoremediation and RNAi tools.

Transgenic Plants

Anonim
Transgenic Plants Book PDF

Author : Anonim
Publisher : Academic Press
Page : 300 pages
File Size : 46,6 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323140263

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Transgenic Plants by Anonim Pdf

Volumes 1 and 2 of Transgenic Plants assemble important information on transgenic crops which has appeared scattered in many different publications. These two volumes are a significant milestone in plant/agricultural biology, promote the practical application of recombinant DNA technology, and assist in transforming the agricultural industry.

Transgenic Plants

Anonim
Transgenic Plants Book PDF

Author : Anonim
Publisher : Academic Press
Page : 403 pages
File Size : 43,9 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323140256

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Transgenic Plants by Anonim Pdf

Volumes 1 and 2 of Transgenic Plants assemble important information on transgenic crops which has appeared scattered in many different publications. These two volumes are a significant milestone in plant/agricultural biology, promote the practical application of recombinant DNA technology, and assist in transforming the agricultural industry.

Transgenic Crop Plants

Chittaranjan Kole,Charles Michler,Albert G. Abbott,Timothy C. Hall
Transgenic Crop Plants Book PDF

Author : Chittaranjan Kole,Charles Michler,Albert G. Abbott,Timothy C. Hall
Publisher : Springer Science & Business Media
Page : 329 pages
File Size : 50,8 Mb
Release : 2010-01-13
Category : Science
ISBN : 9783642048098

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Transgenic Crop Plants by Chittaranjan Kole,Charles Michler,Albert G. Abbott,Timothy C. Hall Pdf

Development of transgenic crop plants, their utilization for improved agriculture, health, ecology and environment and their socio-political impacts are currently important fields in education, research and industries and also of interest to policy makers, social activists and regulatory and funding agencies. This work prepared with a class-room approach on this multidisciplinary subject will fill an existing gap and meet the requirements of such a broad section of readers. Volume 1 with ten chapters contributed by 31 eminent scientists from nine countries deliberates on the basic concepts, strategies and tools for development of transgenic crop plants, including topics such as: explants used for the generation of transgenic plants, gene transfer methods, organelle transformation, selection and screening strategies, expression and stability of transgenes, silencing undesirable genes, transgene integration, biosynthesis and biotransformation and metabolic engineering of pathways and gene discovery.

Genetic Transformation of Plants

John Flex Jackson,Hans F. Linskens
Genetic Transformation of Plants Book PDF

Author : John Flex Jackson,Hans F. Linskens
Publisher : Springer Science & Business Media
Page : 202 pages
File Size : 54,6 Mb
Release : 2013-03-09
Category : Science
ISBN : 9783662074244

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Genetic Transformation of Plants by John Flex Jackson,Hans F. Linskens Pdf

Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy sis have now decided to rename the series Molecular Methods of Plant Analy sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not me an that all chapters apriori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen re cent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis.

Transgenic Plants

Meran R. L. Owen,Jan Pen
Transgenic Plants Book PDF

Author : Meran R. L. Owen,Jan Pen
Publisher : John Wiley & Sons
Page : 378 pages
File Size : 40,5 Mb
Release : 1997-01-23
Category : Science
ISBN : 0471964433

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Transgenic Plants by Meran R. L. Owen,Jan Pen Pdf

Transgenic Plants: A Production System for Industrial andPharmaceutical Proteins provides a detailed guide to the principlesand practice of using transgenic plants as a system for theproduction of heterologous proteins. It is unique in that it coversthe complete process of heterologous protein production in plants,from the initial transformation of the plant, through totranscription, transgene stability and finally the downstreamingprocessing events for protein purification. Written by aninternational team of industrialists and academics, this bookdescribes: * the fundamental issues associated with expressing heterologousproteins in plants; * a number of detailed examples of the successful small-andlarge-scale production of proteins; * the essentials of patenting; and * the commercial exploitation. Transgenic Plants: A Production System for Industrial andPharmaceutical Proteins will be of interest to plantbiotechnologists, molecular biologists and protein biochemists inthe biotechnology and pharmaceutical industries.

Recent Advances in Plant Biotechnology and Its Applications

Ashwani Kumar,Sudhir K. Sopory
Recent Advances in Plant Biotechnology and Its Applications Book PDF

Author : Ashwani Kumar,Sudhir K. Sopory
Publisher : I. K. International Pvt Ltd
Page : 701 pages
File Size : 43,7 Mb
Release : 2008
Category : Plant biotechnology
ISBN : 9788189866099

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Recent Advances in Plant Biotechnology and Its Applications by Ashwani Kumar,Sudhir K. Sopory Pdf

This book is divided into five sections. The first section deals with the methodology and bioresource generation, techniques related to genetic engineering, and gene transfer to the nuclear genome and chloroplast genome. The new techniques of genome profiling and gene silencing are also presented. The second section of the book covers the classical aspect of plant biotechnology viz. tissue culture and micropropagation. Use of genetic engineering via Agrobacterium and direct transfer of DNA through particle bombardment to develop transformed plants in Artemisia, castor and orchids, and production of recombinant proteins in plant cells have been dealt with in the third section. The fourth section addresses the abiotic and biotic stress tolerance in plants. The basic biology of some of the stress responses, and designing plants for stress tolerance is discussed in this section. The fifth section examines medicinal plants and alkaloid production.

Agrobacterium Protocols

Kan Wang
Agrobacterium Protocols Book PDF

Author : Kan Wang
Publisher : Springer Science & Business Media
Page : 474 pages
File Size : 41,9 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597451307

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Agrobacterium Protocols by Kan Wang Pdf

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Transgenic Crops I

Y.P.S. Bajaj
Transgenic Crops I Book PDF

Author : Y.P.S. Bajaj
Publisher : Springer Science & Business Media
Page : 409 pages
File Size : 43,5 Mb
Release : 2012-12-06
Category : Technology & Engineering
ISBN : 9783642596124

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Transgenic Crops I by Y.P.S. Bajaj Pdf

Recently. there has been tremendous progress in the genetic transformation of agricultural crops, and plants resistant to insects, herbicides, and diseases have been produced, field tested and patented. Transgenic Crops I compiles this information on cereals, grasses, legumes, and oilseed crops. It comprises 25 chapters and is divided into two sections: I. Cereals and Grasses: wheat, rice, maize, barley, sorghum, pearl millet, triticale, Agrostis spp., Cenchrus ciliaris, Dactylis glomerata, Festuca arundinacea, Lolium spp., and sugarcane. II. Legumes and Oilseed Crops: Arachis hypogaea, Brassica juncea, Brassica napus, Cicer arietinum, Glycine max, Gossypium hirsutum, Helianthus annuus, Lens culinaris, Linum usitatissimum, Sinapis alba, Trifolium, and Vicis narbonensis. This book is of special interest to advanced students, teachers, and research workers in the field of plant breeding, genetics, molecular biology, plant tissue culture, and plant biotechnology in general.