Genetic Transformation Of Plants

Author : Charles Neal Stewart,Alisher Touraev,Vitaly Citovsky,Tzvi Tzfira
Publisher : John Wiley & Sons
Page : 314 pages
File Size : 44,9 Mb
Release : 2011-01-31
Category : Science
ISBN : 9780470958940

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Plant Transformation Technologies by Charles Neal Stewart,Alisher Touraev,Vitaly Citovsky,Tzvi Tzfira Pdf

Plant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies, Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies. With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.

Author : John Flex Jackson,Hans F. Linskens
Publisher : Springer Science & Business Media
Page : 202 pages
File Size : 40,7 Mb
Release : 2013-03-09
Category : Science
ISBN : 9783662074244

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Genetic Transformation of Plants by John Flex Jackson,Hans F. Linskens Pdf

Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy sis have now decided to rename the series Molecular Methods of Plant Analy sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not me an that all chapters apriori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen re cent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis.

Author : María Alvarez
Publisher : BoD – Books on Demand
Page : 344 pages
File Size : 47,8 Mb
Release : 2011-09-06
Category : Medical
ISBN : 9789533073644

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Genetic Transformation by María Alvarez Pdf

Genetic transformation of plants has revolutionized both basic and applied plant research. Plant molecular biology and physiology benefit from this power fool, as well as biotechnology. This book is a review of some of the most significant achievements that plant transformation has brought to the fields of Agrobacterium biology, crop improvement and, flower, fruit and tree amelioration. Also, it examines their impact on molecular farming, phytoremediation and RNAi tools.

Author : Hau-Hsuan Hwang,Stanton B. Gelvin
Publisher : Frontiers Media SA
Page : 167 pages
File Size : 46,6 Mb
Release : 2015-06-26
Category : Botany
ISBN : 9782889195749

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Agrobacterium biology and its application to transgenic plant production by Hau-Hsuan Hwang,Stanton B. Gelvin Pdf

The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. In this research topic, we provided updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.

Author : National Research Council,Institute of Medicine,Board on Agriculture and Natural Resources,Food and Nutrition Board,Board on Life Sciences,Committee on Identifying and Assessing Unintended Effects of Genetically Engineered Foods on Human Health
Publisher : National Academies Press
Page : 254 pages
File Size : 52,9 Mb
Release : 2004-07-08
Category : Science
ISBN : 9780309166157

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Safety of Genetically Engineered Foods by National Research Council,Institute of Medicine,Board on Agriculture and Natural Resources,Food and Nutrition Board,Board on Life Sciences,Committee on Identifying and Assessing Unintended Effects of Genetically Engineered Foods on Human Health Pdf

Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Author : Wagdy Sawahel
Publisher : Daya Books
Page : 146 pages
File Size : 45,9 Mb
Release : 1997
Category : Plant genetic transformation
ISBN : 8170351758

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Plant Genetic Transformation Technology by Wagdy Sawahel Pdf

The introduction of foreign genetic material into host cells is a vital step in genetic engineering. It is especially important when one considers the potential application of gene transfer systems to crop improvement with the aim of engineering specific traits into a wide variety of plants. The book is an overview of the current research into gene transfer technology and will be valuable for those, who are involved in the field of plant molecular biology, genetics, biochemistry, physiology and biotechnology. Contents Chapter 1: Genetic Transformation; History & Definition, Gene transfer systems, Natural transformation system (vector system), Direct gene transfer (vector-free systems), Genetic transformation strategy, Biological parameters, Requirements for genetic transformation, Arrangement of foreign DNA in the plant genome, Stability of the foreign gene, Modes of genetic recombination, Genetic transformation approaches, Classes of transformants, Inter-transformant variability; Chapter 2: Gene Delivery systems; Polycation-mediated transformation, Particle gun, Electroporation, Microinjection, U V laser microbeam, Electroinjection, Electrophoresis, Protoplast fusion, Macroinjection, Liposome system, Ca-DNA co-precipitation method, Silicon carbide fiber-vortex, Sonication; Chapter 3: Strategies for Improving Transformation Efficiency; Plasmid DNA, Carrier DNA, DNA repair, Transformation of synchronized protoplasts, Restriction-enzyme mediated event, Transformation booster sequence; Chapter 4: Organelle Transformation; Chapter 5: Shotgun Transformation; Plasmid rescue, Gene rescue, Promoter & enhancer rescue.

Author : G. W. Lycett,D. Grierson
Publisher : Elsevier
Page : 304 pages
File Size : 48,8 Mb
Release : 2013-10-22
Category : Technology & Engineering
ISBN : 9781483100111

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Genetic Engineering of Crop Plants by G. W. Lycett,D. Grierson Pdf

Genetic Engineering of Crop Plants is a proceeding of The 49th Nottingham Easter School in Agricultural Science, which was held at Sutton Bonington on April 17-21, 1989. This symposium discussed progress in the generation of crop species resistant to herbicides, viruses, and insects. The book discusses topics such as the genetic manipulation in plants; genetic engineering of crops for insect and herbicide resistance; the expression of heat shock gene in transgenic plants; and tuber-specific gene expression. The book also covers topics such as regulation of gene expression in transgenic tomato plants; the molecular biology of pea seed development; and the regulatory elements of maize storage protein genes. The text is recommended for experts in the field of botany, agriculture, and genetics who would like to know more about the improvement of crop plants through genetics.

Author : Kin-Ying To
Publisher : BoD – Books on Demand
Page : 136 pages
File Size : 48,5 Mb
Release : 2020-10-07
Category : Medical
ISBN : 9781839624506

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Genetic Transformation in Crops by Kin-Ying To Pdf

Due to rapid population growth, climate change, and decreasing natural resources, growing sufficient crops with high productivity, resistance to abiotic and biotic stresses, and other attractive traits is a major challenge. Conventional breeding methods require time-consuming genetic crosses between different parents for multiple generations. By contrast, plant transformation is defined as the insertion of DNA from any organism into the genome of a plant species, and it is considered to be a powerful tool in plant breeding. This book aims to provide professional state-of-the-art information for basic and applied scientists and plant breeders, focusing on key crop plants. Papers related to the principle and application of Agrobacterium-mediated transformation, step-by-step protocols of DNA delivery to the important crop Brassica oleracea and higher-plant chloroplasts, current progress and prospects of virus-induced gene silencing (VIGS) in higher plants, improvement of grapevine through biotechnology, and public concern of biosafety issues regarding genetically modified organisms (GMOs) are all included in this book. It should be useful for students, breeders, and researchers in the field of transgenic crops around the world.

Author : Aditya Pratap,Jitendra Kumar
Publisher : Springer Science & Business Media
Page : 319 pages
File Size : 54,5 Mb
Release : 2013-11-01
Category : Science
ISBN : 9781461485858

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Alien Gene Transfer in Crop Plants, Volume 1 by Aditya Pratap,Jitendra Kumar Pdf

Genetic engineering and biotechnology along with conventional breeding have played an important role in developing superior cultivars by transferring economically important traits from distant, wild and even unrelated species to the cultivated varieties which otherwise could not have been possible with conventional breeding. There is a vast amount of literature pertaining to the genetic improvement of crops over last few decades. However, the wonderful results achieved by crop scientists in food legumes’ research and development over the years are scattered in different journals of the World. The two volumes in the series ‘Alien Gene Transfer in Crop Plants’ address this issue and offer a comprehensive reference on the developments made in major food crops of the world. These volumes aim at bringing the contributions from globally renowned scientists at one platform in a reader-friendly manner. The 1st volume entitled, ‘Alien Gene Transfer in Crop Plants: Innovations, Methods and Risk Assessment” will deal exclusively with the process and methodology. The contents of this volume have been designed to appraise the readers with all the theoretical and practical aspects of wide hybridization and gene transfer like processes and methods of gene transfer, role of biotechnology with special reference to embryo rescue, genetic transformation, protoplast fusion and molecular marker technology, problems such as cross incompatibility and barriers to distant hybridization and solutions to overcome them. Since wild and weedy relatives of crop plants may have negative traits associated with them, there are always possibilities of linkage drag while transferring alien alleles. Therefore, problems and limitations of alien gene transfer from these species will also be discussed in this series. Further, the associated risks with this and assessment of risks will also be given due weightage.

Author : George C. Khachatourians,Y. H. Hui,Ralph Scorza,Wai-Kit Nip
Publisher : CRC Press
Page : 910 pages
File Size : 50,8 Mb
Release : 2002-03-26
Category : Science
ISBN : 0203910974

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Transgenic Plants and Crops by George C. Khachatourians,Y. H. Hui,Ralph Scorza,Wai-Kit Nip Pdf

With contributions from nearly 130 internationally renowned experts in the field, this reference details advances in transgenic plant construction and explores the social, political, and legal aspects of genetic plant manipulation. It provides analyzes of the history, genetics, physiology, and cultivation of over 30 species of transgenic seeds, fruits, and vegetables. Stressing the impact of genetic engineering strategies on the nutritional and functional benefit of foods as well as on consumer health and the global market economy, the book covers methods of gene marking, transferring, and tagging public perceptions to the selective breeding, hybridization, and recombinant DNA manipulation of food.

Author : Yelda Ozden Çiftçi
Publisher : BoD – Books on Demand
Page : 494 pages
File Size : 43,8 Mb
Release : 2012-03-07
Category : Science
ISBN : 9789535101819

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Transgenic Plants by Yelda Ozden Çiftçi Pdf

Development of efficient transformation protocols is becoming a complementary strategy to conventional breeding techniques for the improvement of crops. Thus, Transgenic Plants - Advances and Limitations covers the recent advances carried on improvement of transformation methods together with assessment of the impact of genetically transformed crops on biosafety. Each chapter has been written by one or more experienced researchers in the field and then carefully edited to ensure throughness and consistency.

Author : R. Walden
Publisher : Unknown
Page : 168 pages
File Size : 46,5 Mb
Release : 1989
Category : Science
ISBN : STANFORD:36105032475381

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Genetic Transformation in Plants by R. Walden Pdf

Author : National Research Council,Board on Agriculture
Publisher : National Academies Press
Page : 97 pages
File Size : 48,8 Mb
Release : 1984-02-01
Category : Science
ISBN : 9780309034340

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Genetic Engineering of Plants by National Research Council,Board on Agriculture Pdf

"The book...is, in fact, a short text on the many practical problems...associated with translating the explosion in basic biotechnological research into the next Green Revolution," explains Economic Botany. The book is "a concise and accurate narrative, that also manages to be interesting and personal...a splendid little book." Biotechnology states, "Because of the clarity with which it is written, this thin volume makes a major contribution to improving public understanding of genetic engineering's potential for enlarging the world's food supply...and can be profitably read by practically anyone interested in application of molecular biology to improvement of productivity in agriculture."

Author : Phetole Mangena
Publisher : CRC Press
Page : 244 pages
File Size : 43,7 Mb
Release : 2022-09-01
Category : Science
ISBN : 9781000638158

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Plant Transformation via Agrobacterium Tumefaciens by Phetole Mangena Pdf

Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes. This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment. Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.

Author : Kan Wang,Alfredo Herrera-Estrella,Marc van Montagu
Publisher : Cambridge University Press
Page : 204 pages
File Size : 52,5 Mb
Release : 2004-01-29
Category : Science
ISBN : 0521548209

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Transformation of Plants and Soil Microorganisms by Kan Wang,Alfredo Herrera-Estrella,Marc van Montagu Pdf

Over the past fifty years plant breeders have achieved impressive improvements in yield, quality and disease resistance. These gains suggest that many more modifications might be introduced if appropriate genes can be identified. Current DNA techniques allow the construction of transgenic plants and this important new book reviews the current state of knowledge. A team of leading researchers provide in-depth reviews at the cutting edge of technology for laboratory techniques for the transformation of important soil microorganisms and recalcitrant plants of economic value. The book is divided into three sections: soil microorganisms; cereal crops; and industrially important plants. The most effective methods used to date are compared, and their merits and limitations discussed. Some chapters emphasise case studies and applications. In cases where obstacles remain to be overcome, an overview of progress to date is given. The book will serve as a general guide and reference tool for those working on transformation in microbiology and plant science.