Agrobacterium Protocols

Author : Kan Wang
Publisher : Springer Science & Business Media
Page : 474 pages
File Size : 50,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597451307

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Agrobacterium Protocols by Kan Wang Pdf

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Author : Kan Wang
Publisher : Springer Science & Business Media
Page : 473 pages
File Size : 44,6 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781597451314

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Agrobacterium Protocols by Kan Wang Pdf

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Author : Kan Wang
Publisher : Humana
Page : 484 pages
File Size : 49,5 Mb
Release : 2006-06-01
Category : Science
ISBN : 1588295362

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Agrobacterium Protocols by Kan Wang Pdf

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Author : Kan Wang
Publisher : Springer
Page : 0 pages
File Size : 44,9 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493945416

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Agrobacterium Protocols by Kan Wang Pdf

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.

Author : Kan Wang
Publisher : Springer
Page : 378 pages
File Size : 48,7 Mb
Release : 2014-11-22
Category : Science
ISBN : 1493916599

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Agrobacterium Protocols by Kan Wang Pdf

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 2 contains 29 chapters with updated techniques for industrial plants, root plants, nuts and fruits, tropic plants, and other important plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers in both fundamental and applied biology.

Author : Leandro Peña
Publisher : Springer Science & Business Media
Page : 427 pages
File Size : 46,9 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592598274

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Transgenic Plants by Leandro Peña Pdf

The aim of Transgenic Plants: Methods and Protocols is to provide a source of information to guide the reader through a wide range of frequently used, broadly applicable, and easily reproducible techniques involved in the gene- tion of transgenic plants. Its step-by-step approach covers a series of methods for genetically transforming plant cells and tissues, and for recovering whole transgenic plants from them. The volume then moves on to the use of sele- able and reporter markers, positive selection, marker elimination after rec- ery of transgenic plants, and the analysis of transgene integration, expression, and localization in the plant genome. Although contributors usually refer to model plants in most chapters, the protocols described herein should be widely applicable to many plant species. The last two sections are devoted to me- ods of risk assessment and to exploring the current and future applications of transgenic technology in agriculture and its social implications in a case study. Transgenic Plants: Methods and Protocols is divided into six major s- tions plus an introduction, comprising 27 chapters. Part I, the Introduction, is a review of the past, present, and perspectives of the transgenic plants, from the discovery of Agrobacterium tumefaciens as a feasible transformation vector, to its use as a tool to study gene expression and function, and the current and possible future applications of this technology in agriculture, industry, and medicine.

Author : Charles Neal Stewart,Alisher Touraev,Vitaly Citovsky,Tzvi Tzfira
Publisher : John Wiley & Sons
Page : 314 pages
File Size : 47,7 Mb
Release : 2011-01-31
Category : Science
ISBN : 9780470958940

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Plant Transformation Technologies by Charles Neal Stewart,Alisher Touraev,Vitaly Citovsky,Tzvi Tzfira Pdf

Plant Transformation Technologies is a comprehensive, authoritative book focusing on cutting-edge plant biotechnologies, offering in-depth, forward-looking information on methods for controlled and accurate genetic engineering. In response to ever-increasing pressure for precise and efficient integration of transgenes in plants, many new technologies have been developed. With complete coverage of these technologies, Plant Transformation Technologies provides valuable insight on current and future plant transformation technologies. With twenty-five chapters written by international experts on transformation technologies, the book includes new information on Agrobacterium, targeting transgenes into plant genomes, and new vectors and market systems. Including both review chapters and protocols for transformation, Plant Transformation Technologies is vitally important to graduate students, postdoctoral students, and university and industry researchers.

Author : Ian S. Curtis
Publisher : Springer Science & Business Media
Page : 470 pages
File Size : 47,8 Mb
Release : 2004-11-30
Category : Technology & Engineering
ISBN : 1402023324

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Transgenic Crops of the World by Ian S. Curtis Pdf

Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants.

Author : Sandeep Kumar,Pierluigi Barone,Michelle Smith
Publisher : Unknown
Page : 444 pages
File Size : 40,6 Mb
Release : 2018
Category : Plant genetics
ISBN : 149398778X

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Transgenic Plants by Sandeep Kumar,Pierluigi Barone,Michelle Smith Pdf

Author : Heddwyn Jones
Publisher : U of Nebraska Press
Page : 500 pages
File Size : 55,5 Mb
Release : 1995-09-27
Category : Science
ISBN : 089603321X

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Plant Gene Transfer and Expression Protocols by Heddwyn Jones Pdf

Presents step-by-step recipes for introducing genes into model and agricultural plants and for studying gene activity, and contains mainstream methods and methods for studying gene expression and genetic manipulation in chloroplasts and mitochondria. Sections cover agrobacterium-mediated transformation, direct gene transfer, use of reporter genes, southern blotting, and inverse PCR. Each chapter ends with a notes section highlighting potential problems and providing tips and alternatives. Plastic comb-binding. Annotation copyright by Book News, Inc., Portland, OR

Author : Zhiqiang An
Publisher : CRC Press
Page : 785 pages
File Size : 49,9 Mb
Release : 2004-08-30
Category : Science
ISBN : 9780203970553

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Handbook of Industrial Mycology by Zhiqiang An Pdf

Several excellent books have been published that address one or more aspects of the diverse field of industrial mycology, but none of them cover the entire process of fungal bioactive metabolites discovery. Until now. The Handbook of Industrial Mycology provides, in one volume, an overview of recent developments in industrial mycology with emphasis on the discovery of bioactive metabolites and, most importantly, their underlying biology and genetics. Two additional features distinguish this book from other books in the field: 1) most chapters are prepared using experimental data to illustrate theories and 2) the authors provide methodologies and experimental protocols in their chapters. Presenting a comprehensive overview of recent advances, the book provides a framework of basic methods, tools, and organizational principles for channeling fungal germplasm into the academic, pharmaceutical, and enzyme discovery laboratories. It covers the complex range of processes involved in the discovery, characterization, and profiling of bioactive fungal metabolites. The book includes examples of several recently marketed fungal metabolites and explores the impact of fungi on applications in the pharmaceutical, food and beverage, agricultural, and agrochemical industries.

Author : Kin-Ying To
Publisher : BoD – Books on Demand
Page : 136 pages
File Size : 55,7 Mb
Release : 2020-10-07
Category : Medical
ISBN : 9781839624506

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Genetic Transformation in Crops by Kin-Ying To Pdf

Due to rapid population growth, climate change, and decreasing natural resources, growing sufficient crops with high productivity, resistance to abiotic and biotic stresses, and other attractive traits is a major challenge. Conventional breeding methods require time-consuming genetic crosses between different parents for multiple generations. By contrast, plant transformation is defined as the insertion of DNA from any organism into the genome of a plant species, and it is considered to be a powerful tool in plant breeding. This book aims to provide professional state-of-the-art information for basic and applied scientists and plant breeders, focusing on key crop plants. Papers related to the principle and application of Agrobacterium-mediated transformation, step-by-step protocols of DNA delivery to the important crop Brassica oleracea and higher-plant chloroplasts, current progress and prospects of virus-induced gene silencing (VIGS) in higher plants, improvement of grapevine through biotechnology, and public concern of biosafety issues regarding genetically modified organisms (GMOs) are all included in this book. It should be useful for students, breeders, and researchers in the field of transgenic crops around the world.

Author : Yasunari Ogihara,Shigeo Takumi,Hirokazu Handa
Publisher : Springer
Page : 445 pages
File Size : 53,7 Mb
Release : 2015-09-15
Category : Science
ISBN : 9784431556756

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Advances in Wheat Genetics: From Genome to Field by Yasunari Ogihara,Shigeo Takumi,Hirokazu Handa Pdf

This proceedings is a collection of 46 selected papers that were presented at the 12th International Wheat Genetics Symposium (IWGS). Since the launch of the wheat genome sequencing project in 2005, the arrival of draft genome sequences has marked a new era in wheat genetics and genomics, catalyzing rapid advancement in the field. This book provides a comprehensive review of the forefront of wheat research, across various important topics such as germplasm and genetic diversity, cytogenetics and allopolyploid evolution, genome sequencing, structural and functional genomics, gene function and molecular biology, biotic stress, abiotic stress, grain quality, and classical and molecular breeding. Following an introduction, 9 parts of the book are dedicated to each of these topics. A final, 11th part entitled “Toward Sustainable Wheat Production” contains 7 excellent papers that were presented in the 12th IWGS Special Session supported by the OECD. With rapid population growth and radical climate changes, the world faces a global food crisis and is in need of another Green Revolution to boost yields of wheat and other widely grown staple crops. Although this book focuses on wheat, many of the newly developed techniques and results presented here can be applied to other plant species with large and complex genomes. As such, this volume is highly recommended for all students and researchers in wheat sciences and related plant sciences and for those who are interested in stable food production and food security.

Author : Julian Coleman,David Evans,Anne Kearns
Publisher : Taylor & Francis
Page : 212 pages
File Size : 48,7 Mb
Release : 2020-07-26
Category : Nature
ISBN : 9781000144710

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Plant Cell Culture by Julian Coleman,David Evans,Anne Kearns Pdf

Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory's bookshelf.

Author : Julio Salinas,Jose J. Sanchez-Serrano
Publisher : Springer Science & Business Media
Page : 461 pages
File Size : 40,6 Mb
Release : 2008-02-04
Category : Nature
ISBN : 9781597450034

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Arabidopsis Protocols, 2nd Edition by Julio Salinas,Jose J. Sanchez-Serrano Pdf

For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge.