Production Of Biologicals From Animal Cells In Culture

Author : R. E. Spier,J. B. Griffiths,B. Meignier
Publisher : Butterworth-Heinemann
Page : 853 pages
File Size : 55,6 Mb
Release : 2013-09-24
Category : Medical
ISBN : 9781483165158

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Production of Biologicals from Animal Cells in Culture by R. E. Spier,J. B. Griffiths,B. Meignier Pdf

Production of Biologicals from Animal Cells in Culture reviews the state of the art in animal cell biotechnology, with emphasis on the sequence of events that occur when generating a biological from animal cells in culture. Methods that enable adjustment of nutrient feed streams into perfusion bioreactors so as to increase productivity are described. A number of issues are also addressed, such as the usefulness of the fingerprint method for cell characterization. Comprised of 135 chapters, this book begins with an overview of the problems and benefits of animal cell culture, followed by a discussion on the isolation of immortal murine macrophage cell lines. The reader is systematically introduced to the use of DNA fingerprinting to characterize cell banks; immortalization of cells with oncogenes; lipid metabolism of animal cells in culture; and energetics of glutaminolysis. Subsequent chapters explore serum-free and protein-free media; the physiology of animal cells; gene expression in animal cell systems; and animal cell bioreactors. The monitoring and assay of animal cell parameters are also considered, along with downstream processing and regulatory issues. This monograph will be of interest to students, practitioners, and investigators in the fields of microbiology and biotechnology.

Author : Hansjörg Hauser,Roland Wagner
Publisher : Walter de Gruyter GmbH & Co KG
Page : 715 pages
File Size : 48,8 Mb
Release : 2014-11-10
Category : Science
ISBN : 9783110381429

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Animal Cell Biotechnology by Hansjörg Hauser,Roland Wagner Pdf

This book introduces fundamental principles and practical application of techniques used in the scalable production of biopharmaceuticals with animal cell cultures. A broad spectrum of subjects relevant to biologics production and manufacturing are reviewed, including the generation of robust cell lines, a survey of functional genomics for a better understanding of cell lines and processes, as well as advances in regulatory compliant upstream and downstream development. The book is an essential reference for all those interested in translational animal cell-based pharmaceutical biotechnology.

Author : Ryuzo Sasaki,Kouji Ikura
Publisher : Springer Science & Business Media
Page : 427 pages
File Size : 54,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401135504

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Animal Cell Culture and Production of Biologicals by Ryuzo Sasaki,Kouji Ikura Pdf

In the past two decades, the importance of animal cell technology has increased enormously. First, useful proteins can be produced by cultured animal cells, in which the desired product can be modified and organized so as to retain its biological function. Second, studies of cultured cells can provide information needed to understand molecular mechanisms that govern what happens in tissues, organs, and even entire organisms. For this second purpose, biochemists and molecular biologists may need a large number of such cells. Third, cultured cells can be used instead of tissues and organs clinically. The Third Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), at which participants from abroad were warmly welcomed, was held in Kyoto on December 11-13, 1990. It was organized around the idea of providing a place for the review of much new data on such applications of cultured cells and for exchanges of the views of the participants about progress in the field. This volume, divided into seven sections, contains the proceedings of the meeting. The first section reviews the molecular basis of the control of animal cell growth. In the following sections, physicochemical and biochemical factors for cell growth and production of biologicals, cell culture systems including serum-free culture, new cell lines, specific products and their characteristics, and in vitro assays for toxic, carcinogenic, and pharmacological effects are taken up in their tum.

Author : Mohamed Al-Rubeai
Publisher : Springer
Page : 766 pages
File Size : 49,9 Mb
Release : 2014-11-28
Category : Medical
ISBN : 9783319103204

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Animal Cell Culture by Mohamed Al-Rubeai Pdf

​Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.

Author : Terence Cartwright
Publisher : Cambridge University Press
Page : 193 pages
File Size : 45,7 Mb
Release : 1994-08-26
Category : Medical
ISBN : 9780521412582

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Animal Cells as Bioreactors by Terence Cartwright Pdf

Because of their complexity, the new generation of genetically engineered protein drugs can only be made by biotechnological methods, using cultures of animal cells. This book covers all aspects of the technologies needed to turn animal cells into an acceptable and cost-effective tool for drug production. This includes modifying them genetically so that they produce the right product in high yield, getting them to grow reproducibly on an industrial scale, and extracting the required product from them. It also covers biological safety issues, and the verification of the chemical and biological nature of the protein drug produced. The work covers developments in all of these areas and how they all need to be integrated for the design of an effective biotechnological production process. It therefore provides a comprehensive guide to this area of biotechnology.

Author : J. P. Barford
Publisher : NUS Press
Page : 288 pages
File Size : 55,6 Mb
Release : 1995
Category : Science
ISBN : 9971691949

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Fundamental and Applied Aspects of Animal Cell Cultivation by J. P. Barford Pdf

The advent of modern, biological techniques such as hybridoma technology, recombinant DNA techniques and viral transformation of cells has made the continuous production of a wide variety of biologicals possible using animal cells. The use of such products is well established in many diagnostic and (increasingly) therapeutic applications - the U.S. market for antibodies, for example, has been projected to increase from a 1991 level of US$0.33 billion to 1998 level of US$3.8 billion. Total sales of such products in 1992 was US$4.2 billion. The increasing application of this technology depends on increasing the efficiency of production and bioseparation and addressing various safety issues. This book examines the fundamental and applied aspects of animal cell cultivation.

Author : Raymond Spier,R. E. Spier,J. B. Griffiths
Publisher : Unknown
Page : 358 pages
File Size : 54,5 Mb
Release : 1985
Category : Science
ISBN : PSU:000021836381

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Animal Cell Biotechnology by Raymond Spier,R. E. Spier,J. B. Griffiths Pdf

The Animal Cell Biotechnology series provides an in-depth state-of-the art coverage of a field which has developed into a multi-billion dollar industry over the last ten years. Written by leading experts, each volume contains well-referenced, fully-indexed reviews on practical and theoretical aspects of a specific subject, including: Bioreactor design and development Cell products and their development Downstream processing Product testing The main theme of this volume - applications of animal cells - has been broadened from the recurring theme of production of biologicals to the cells themselves as a product, and cells as a pharmacological/toxicological assay system. The need to replace animals in research and for the testing of drugs, foods and cosmetics is becoming increasingly critical as society has succeeded in making this a high profile ethical issue. It is the perceived belief that the best chance of reducing the use of animals will be to develop suitable assays based on cells in culture. Two chapters in this volume cover replacement animal cells and the use of skin cells for in vitro testing. Other chapters also detail the cell products tPA, Factor VIII, and Growth Factors. The basic tool is, of course, the cell and the text contains chapters on how to standardize the cell and process in order to achieve reproducible conditions, whether for production or assay purposes. Also featured is a review of immortalization techniques that can be used to construct new cell lines which fulfill the specialized criteria required of them. Finally a chapter on surface active agents is included.

Author : Weichang Zhou,Anne Kantardjieff
Publisher : Springer
Page : 262 pages
File Size : 47,6 Mb
Release : 2014-01-15
Category : Science
ISBN : 9783642540509

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Mammalian Cell Cultures for Biologics Manufacturing by Weichang Zhou,Anne Kantardjieff Pdf

Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Author : R. E. Spier,J. B. Griffiths,C. Macdonald
Publisher : Butterworth-Heinemann
Page : 785 pages
File Size : 47,8 Mb
Release : 2013-10-02
Category : Technology & Engineering
ISBN : 9781483145075

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Animal Cell Technology by R. E. Spier,J. B. Griffiths,C. Macdonald Pdf

Animal Cell Technology: Developments, Processes and Products is a compilation of scientific papers presented at the 11th European Society for Animal Cell Technology (ESACT) Meeting, held in Brighton, United Kingdom. The book is a collection of various works of scientists, engineers, and other specialists from Europe and other parts of the world who are working with animal cells. The book’s aim is to communicate experiences and research findings on the development of cell systems. The research papers are grouped into 25 sections encompassing 145 chapters. Subjects covered range from cells and physiology engineering dealing with cell characterization, cell culture establishment, cloning, and cell engineering. Topics on culture media, ammonium detoxification, the effects of physical parameters on cell cultures, assays and monitoring systems, and bioreactor techniques are also covered. Discussions are likewise made on the products from animal cells in culture, virus removal, and DNA determination and characterization in relation to safety issues. The book will be useful for cell biologists, molecular biologists, biochemists, biochemical engineers, and students engaged in the study of animal cell cultures.

Author : Luigi Salvay
Publisher : Unknown
Page : 304 pages
File Size : 45,9 Mb
Release : 2017-02
Category : Electronic
ISBN : 1681176955

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Bioreactors by Luigi Salvay Pdf

Author : Moshe D. White,Shaul Reuveny,Avigdor Shafferman
Publisher : John Wiley & Sons
Page : 596 pages
File Size : 46,9 Mb
Release : 1991
Category : Medical
ISBN : UOM:39015029280081

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Biologicals from Recombinant Microorganisms and Animal Cells by Moshe D. White,Shaul Reuveny,Avigdor Shafferman Pdf

These Proceedings comprise contributions from scientists and engineers in academia and industry on practical aspects of production and purification of recombinant proteins. International, practice-oriented and detailed, the book is divided into four parts: - Choosing a production system - Propagation of recombinant microorganisms and animal cells - Purification of recombinant proteins - Health care and veterinary recombinant products Moreover, the book is interdisciplinary. This ensures that all important aspects of the production of recombinant proteins are considered. Genetic engineers, biochemical engineers, microbiologists, virologists and biochemists, protein chemists and quality control experts will find this reference book to be an indispensable guide for their daily work.

Author : Ralf Pörtner
Publisher : Humana
Page : 0 pages
File Size : 52,8 Mb
Release : 2019-12-20
Category : Science
ISBN : 1071601903

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Animal Cell Biotechnology by Ralf Pörtner Pdf

Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Author : Open Universiteit (Heerlen, Netherlands),University of Greenwich
Publisher : Butterworth-Heinemann
Page : 312 pages
File Size : 53,6 Mb
Release : 1993
Category : Science
ISBN : UOM:39015032915103

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In Vitro Cultivation of Animal Cells by Open Universiteit (Heerlen, Netherlands),University of Greenwich Pdf

Both practical and theoretical issues of animal cell cultivation are described, including media formulation, the production and characterisation of cell issues from explants and the preservation of cell lines. The book investigates how pure cultures of animal cells may be isolated from their primary sources, examines the parameters which influence their growth in culture and explores how such parameters may be manipulated to modify cell yields.

Author : Glyn N. Stacey,John Davis
Publisher : Wiley
Page : 0 pages
File Size : 46,9 Mb
Release : 2007-07-16
Category : Science
ISBN : 0470850949

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Medicines from Animal Cell Culture by Glyn N. Stacey,John Davis Pdf

Medicines from Animal Cell Culture focuses on the use of animal cell culture, which has been used to produce human and veterinary vaccines, interferon, monoclonal antibodies and genetically engineered products such as tPA and erythropoietin. It also addresses the recent dramatic expansion in cell-based therapies, including the use of live cells for tissue regeneration and the culture of stem cells. Medicines from Animal Cell Culture: Provides comprehensive descriptions of methods for cell culture and nutrition as well as the technologies for the preservation and characterisation of both the cells and the derived products Describes the preparation of stem cells and others for use in cell-based therapies – an area of burgeoning research Includes experimental examples to indicate expected results Covers regulatory issues from the UK, the EU and the USA and reviews how these are developing around the world Addresses the key issues of standardisation and validation with chapters on GLP and GMP for cell culture processes Delivering insight into the exciting world of biological medicines and directions for further investigation into specific topics, Medicines from Animal Cell Culture is an essential resource for researchers and technicians at all levels using cell culture within the pharmaceutical, biotechnology and biomedical industries. It is of value to laboratory managers in these industries and to all those interested in this topic alike.

Author : Michael Butler
Publisher : Unknown
Page : 128 pages
File Size : 49,8 Mb
Release : 2003
Category : Electronic
ISBN : OCLC:1099334053

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Animal Cell Culture and Technology by Michael Butler Pdf

Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduct.