The Science And Application Of Aqueous Two Phase Systems And Liquid Liquid Phase Separation In Biotechnology And Bioengineering

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The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering

John Paul Frampton,Brendan M. Leung,Anderson Ho Cheung Shum
The Science and Application of Aqueous Two Phase Systems and Liquid Liquid Phase Separation in Biotechnology and Bioengineering Book PDF

Author : John Paul Frampton,Brendan M. Leung,Anderson Ho Cheung Shum
Publisher : Frontiers Media SA
Page : 117 pages
File Size : 45,6 Mb
Release : 2020-01-13
Category : Electronic
ISBN : 9782889633562

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The Science and Application of Aqueous Two-Phase Systems and Liquid-Liquid Phase Separation in Biotechnology and Bioengineering by John Paul Frampton,Brendan M. Leung,Anderson Ho Cheung Shum Pdf

The phase separation of incompatible liquids has been a topic of significant importance in chemical and industrial engineering for many years. Well-understood examples of this phenomenon include the phase separation of oil with water and the phase separation of non-polar organic solvents with water. Similar behavior is observed when aqueous solutions of two or more incompatible polymers or polymers and salts are mixed. In these mixtures (referred to as aqueous two-phase systems), the separated phases are composed mostly of water. Aqueous two-phase systems have been used extensively for the extraction of high-value biological products from mixtures of biological materials. In recent years, aqueous two-phase systems have also found increased use as materials for streamlining and improving the capabilities of cell and molecular assays, and for the design of advanced cell culture systems. Similar behavior of biological materials in living systems has also been observed, with emerging roles in cell physiology.

Aqueous Two-Phase Systems

Rajni Hatti-Kaul
Aqueous Two Phase Systems Book PDF

Author : Rajni Hatti-Kaul
Publisher : Springer Science & Business Media
Page : 442 pages
File Size : 40,9 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590285

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Aqueous Two-Phase Systems by Rajni Hatti-Kaul Pdf

A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.

Partitioning In Aqueous Two – Phase System

Harry Walter
Partitioning In Aqueous Two Phase System Book PDF

Author : Harry Walter
Publisher : Elsevier
Page : 730 pages
File Size : 43,8 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323142915

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Partitioning In Aqueous Two – Phase System by Harry Walter Pdf

Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

Liquid Biphasic System

Pau Loke Show,Sze Ying Lee,Kit Wayne Chew
Liquid Biphasic System Book PDF

Author : Pau Loke Show,Sze Ying Lee,Kit Wayne Chew
Publisher : Elsevier
Page : 330 pages
File Size : 55,7 Mb
Release : 2020-11-28
Category : Technology & Engineering
ISBN : 9780128223987

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Liquid Biphasic System by Pau Loke Show,Sze Ying Lee,Kit Wayne Chew Pdf

Downstream bioprocesses have a significant role to play in the creation of a sustainable bio-based economy, enabling the creation of new products and systems from the more sustainable bioprocessing of natural products. Liquid Biphasic System: Fundamentals, Methods, and Applications in Bioseparation Technology explores in detail the fundamental processes and applications of this new separation system, aiding understanding of the basic principles of the technique and offering constructive criticisms on the latest findings. Including coverage of the background, principles, mechanisms, and applications, Liquid Biphasic System addresses how to adapt the technology for the purification of useful compounds with greater cost efficiency and greener processing. It is essential reading for bioprocess engineers, biochemical engineers, biosystem engineers, chemists and microbiologists working in the fields of bioprocessing. Researchers, scientists, and engineers concerned with the selection and evaluation of alternative bioseparation processes will find the book particularly useful. Provides information and examples of advanced separations in a single source Includes detailed descriptions of novel bioseparation systems Covers the latest technologies related to advanced liquid–liquid separation and their applications in various industries

Separations Using Aqueous Phase Systems

D. Fisher
Separations Using Aqueous Phase Systems Book PDF

Author : D. Fisher
Publisher : Springer Science & Business Media
Page : 476 pages
File Size : 52,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468456677

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Separations Using Aqueous Phase Systems by D. Fisher Pdf

The use of aqueous two-phase systems for the partitioning of macromolecules, organelles and cells was originally developed by Per-Ake Albertsson in Sweden in the mid-fifties [1-3]. These systems were initially applied to separations of plant organelles and viruses but their use has now extended into most areas of cell biology and biochemistry [4,5]. Since 1979 biennial International Conferences on Partitioning in Aqueous Two-Phase Systems have been held in Los Angeles (1979), Sheffield (1981), Vancouver (1983) and Lund (1985). The 5th conference was held in Oxford from 23-28 August 1987 and was entitled "Advances in Separations Using Aqueous Phase Systems in Cell Biology and Biotechnology". It is the formal presentations from this meeting which comprise this volume. In contrast to earlier books on phase partitioning [4,5] this volume contains, for the first time, worldwide contributions from over sixty partitioners from a variety of scientific disciplines, thereby providing a detailed overview of the widespread application and potential of bioseparations using phase partitioning. Disciplines include Biophysics, Biochemistry, Cell Biology, Microbiology, Biotechnology and Process Engineering, in both academic and commercial establishments. These biennial conferences allow advances in these diverse partitioning fields to be reviewed and compared; they also provide an opportunity for those considering using phase partitioning to obtain information, advice and contacts. Attendance has grown steadily over the years and 140 scientists came to Oxford. The conference consisted of ten symposia on areas of application of partitioning which have been organised as specific chapters in this volume.

Droplets of Life

Vladimir N Uversky
Droplets of Life Book PDF

Author : Vladimir N Uversky
Publisher : Academic Press
Page : 730 pages
File Size : 52,8 Mb
Release : 2022-11-09
Category : Science
ISBN : 9780128241752

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Droplets of Life by Vladimir N Uversky Pdf

Droplets of Life: Membrane-Less Organelles, Biomolecular Condensates, and Biological Liquid–Liquid Phase Separation provides foundational information on the biophysics, biogenesis, structure, functions, and roles of membrane-less organelles. The study of liquid–liquid phase separation has attracted a lot of attention from disciplines such as cell biology, biophysics, biochemistry, and others trying to understand how, why, and what roles these condensates play in homeostasis and disease states in living organisms. This book's editor recruited a group of international experts to provide a current and authoritative overview of all aspects associated with this exciting area. Sections introduce membrane-less organelles (MLOs) and biomolecular condensates; MLOs in different sizes, shapes, and composition; and the formation of MLOs due to phase separation and how it can tune reactions, organize the intracellular environment, and provide a role in cellular fitness. . Presents the first book to establish the foundations of this exciting research area Combines biophysics, structural and cell biology, and biochemistry perspectives into a single volume Edited and authored by world-leading scientists Covers basic physical and biological principles and health and disease implications

Current Developments in Biotechnology and Bioengineering

Ashok Pandey,Ranjna Sirohi,Christian Larroche,Mohammad Taherzadeh
Current Developments in Biotechnology and Bioengineering Book PDF

Author : Ashok Pandey,Ranjna Sirohi,Christian Larroche,Mohammad Taherzadeh
Publisher : Elsevier
Page : 523 pages
File Size : 51,6 Mb
Release : 2022-08-18
Category : Technology & Engineering
ISBN : 9780323984836

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Current Developments in Biotechnology and Bioengineering by Ashok Pandey,Ranjna Sirohi,Christian Larroche,Mohammad Taherzadeh Pdf

Advances in Bioprocess Engineering, the latest release in the Current Developments in Biotechnology and Bioengineering series, provides a comprehensive overview of bioprocess systems, kinetics, bioreactor design, batch and continuous reactors and introduces key principles that enable bioprocess engineers to engage in analysis, optimization and design with consistent control over biological and chemical transformations. The bioprocessing sector is also updating its technologies with state-of-the art techniques to keep up with the rising demand of the industry and R&D. This book covers these aspects, taking readers through a step-by-step journey of bioprocessing while also guiding them towards a new era and future. Covers state-of-the-art, technological advancements in the field of bioprocessing Includes design and scale-up of bioreactors, monitoring and control systems, advances in upstream and downstream processing Includes design and development of fermentation processes such as the suitability of experimental design, full factorial, central composite design, Box-Behnken, Plackett-Burman, and more

Aqueous Two-Phase Systems

Rajni Hatti-Kaul
Aqueous Two Phase Systems Book PDF

Author : Rajni Hatti-Kaul
Publisher : Unknown
Page : 128 pages
File Size : 54,5 Mb
Release : 2000
Category : Electronic
ISBN : OCLC:748539223

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Aqueous Two-Phase Systems by Rajni Hatti-Kaul Pdf

Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins - including a glimpse of large-scale handling of two-phase separations. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

Applications of Ionic Liquids in Science and Technology

Scott Handy
Applications of Ionic Liquids in Science and Technology Book PDF

Author : Scott Handy
Publisher : BoD – Books on Demand
Page : 532 pages
File Size : 40,6 Mb
Release : 2011-09-22
Category : Science
ISBN : 9789533076058

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Applications of Ionic Liquids in Science and Technology by Scott Handy Pdf

This volume, of a two volume set on ionic liquids, focuses on the applications of ionic liquids in a growing range of areas. Throughout the 1990s, it seemed that most of the attention in the area of ionic liquids applications was directed toward their use as solvents for organic and transition-metal-catalyzed reactions. Certainly, this interest continues on to the present date, but the most innovative uses of ionic liquids span a much more diverse field than just synthesis. Some of the main topics of coverage include the application of RTILs in various electronic applications (batteries, capacitors, and light-emitting materials), polymers (synthesis and functionalization), nanomaterials (synthesis and stabilization), and separations. More unusual applications can be noted in the fields of biomass utilization, spectroscopy, optics, lubricants, fuels, and refrigerants. It is hoped that the diversity of this volume will serve as an inspiration for even further advances in the use of RTILs.

Protein Purification Process Engineering

Roger Harrison
Protein Purification Process Engineering Book PDF

Author : Roger Harrison
Publisher : Routledge
Page : 398 pages
File Size : 54,6 Mb
Release : 2019-07-16
Category : Medical
ISBN : 9781351421010

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Protein Purification Process Engineering by Roger Harrison Pdf

Offers coverage of the development of protein purification processes for large-scale commercial operations, and addresses process development, scale-up, applications and mathematical descriptions. Technologies currently used at the commercial scale are covered in depth.

Comprehensive Biotechnology

Anonim
Comprehensive Biotechnology Book PDF

Author : Anonim
Publisher : Newnes
Page : 5304 pages
File Size : 52,6 Mb
Release : 2011-08-26
Category : Science
ISBN : 9780080885049

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Comprehensive Biotechnology by Anonim Pdf

The second edition of Comprehensive Biotechnology, Six Volume Set continues the tradition of the first inclusive work on this dynamic field with up-to-date and essential entries on the principles and practice of biotechnology. The integration of the latest relevant science and industry practice with fundamental biotechnology concepts is presented with entries from internationally recognized world leaders in their given fields. With two volumes covering basic fundamentals, and four volumes of applications, from environmental biotechnology and safety to medical biotechnology and healthcare, this work serves the needs of newcomers as well as established experts combining the latest relevant science and industry practice in a manageable format. It is a multi-authored work, written by experts and vetted by a prestigious advisory board and group of volume editors who are biotechnology innovators and educators with international influence. All six volumes are published at the same time, not as a series; this is not a conventional encyclopedia but a symbiotic integration of brief articles on established topics and longer chapters on new emerging areas. Hyperlinks provide sources of extensive additional related information; material authored and edited by world-renown experts in all aspects of the broad multidisciplinary field of biotechnology Scope and nature of the work are vetted by a prestigious International Advisory Board including three Nobel laureates Each article carries a glossary and a professional summary of the authors indicating their appropriate credentials An extensive index for the entire publication gives a complete list of the many topics treated in the increasingly expanding field

Engineering Aspects of Milk and Dairy Products

Jane Selia dos Reis Coimbra,Jose A. Teixeira
Engineering Aspects of Milk and Dairy Products Book PDF

Author : Jane Selia dos Reis Coimbra,Jose A. Teixeira
Publisher : CRC Press
Page : 275 pages
File Size : 52,6 Mb
Release : 2016-04-19
Category : Technology & Engineering
ISBN : 9781420090390

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Engineering Aspects of Milk and Dairy Products by Jane Selia dos Reis Coimbra,Jose A. Teixeira Pdf

Expert Insight into the Engineering Aspects of Dairy Products ManufacturingConsumer demand is constantly on the rise for better and more nutritious dairy products, from traditional milk to new, high-value added products like meal-replacement drinks. This changing market preference reinforces the importance of milk as a raw material in the food indu

Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products

Marco Rito-Palomares,Jorge Benavides
Aqueous Two Phase Systems for Bioprocess Development for the Recovery of Biological Products Book PDF

Author : Marco Rito-Palomares,Jorge Benavides
Publisher : Springer
Page : 191 pages
File Size : 42,9 Mb
Release : 2017-07-18
Category : Technology & Engineering
ISBN : 9783319593098

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Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products by Marco Rito-Palomares,Jorge Benavides Pdf

This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.

Purification of Biotechnological Products

Adalberto Pessoa Jr,Beatriz Vahan Kilikian,Paul Long
Purification of Biotechnological Products Book PDF

Author : Adalberto Pessoa Jr,Beatriz Vahan Kilikian,Paul Long
Publisher : CRC Press
Page : 539 pages
File Size : 41,8 Mb
Release : 2024-05-23
Category : Medical
ISBN : 9781040017661

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Purification of Biotechnological Products by Adalberto Pessoa Jr,Beatriz Vahan Kilikian,Paul Long Pdf

This outstanding text focuses on providing professionals and students working in the pharmaceutical and biotechnology field with the background necessary for developing of a product or process and with the necessary rigor required by federal regulatory agencies in the pharmaceutical industry. The material will enable teachers, lecturers and professors in biotechnology to prepare courses on basic concepts and applications for the purification of biotechnological products of industrial interest. These can be applied in practice, for example, with projects on purification development on an industrial scale or useful unit operations for the development of bioproducts of commercial interest. Features: Purification and development of new bioproducts and improvement of those being produced Provides a background and concepts on the purification of biomolecules and with an industrial perspective It allows professionals to understand the entire process of developing a biopharmaceutical or bio-food, from bench to industry in biotechnology; one of the fastest-growing sectors of the economy It promotes the dissemination of information in a didactic way which is of paramount importance for interdisciplinary fields It enables the reader to follow step-by-step stages of the development of a new biopharmaceutical, and allows the optimization of existing processes

Biopolymer Engineering in Food Processing

Vania Regina Nicoletti Telis
Biopolymer Engineering in Food Processing Book PDF

Author : Vania Regina Nicoletti Telis
Publisher : CRC Press
Page : 419 pages
File Size : 46,9 Mb
Release : 2012-05-29
Category : Science
ISBN : 9781439844946

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Biopolymer Engineering in Food Processing by Vania Regina Nicoletti Telis Pdf

Due to their unique properties and ability to interact with other food components, biopolymers have traditionally played a major role in food processing. Biopolymer Engineering in Food Processing explores processing technology associated with biopolymer applications and discusses both operational and economic aspects. Following an overview of biopolymer applications and their functionality in different processes, the text examines: Production routes, availability, costs, and physicochemical properties of commercial biopolymers Rheology of biopolymer suspensions, how concentration and shear may affect their flow behavior, and their response to pressure losses and heat transfer during flow Effects of food processing and storage conditions on the viscoelastic and textural properties of food gels Mechanical and mass transfer properties of films and coating produced from biopolymers, composites, and nanocomposites The use of biopolymer coatings to reduce oil uptake during deep-fat frying of foods and in modified atmosphere storage of foods The book also explores the application of biopolymers in separation processes for recovery of biocompounds. It discusses biopolymer behavior during thermoplastic extrusion and the response of certain cereals and snacks to extrusion operating parameters. Finally, it reviews engineering aspects of biopolymers used as drying aids in spray-drying and freeze-drying of fruit juices and pulps and discusses biopolymers used as cryoprotectants in food freezing. A comprehensive source of scientific and technical information for those involved with process design and research and development, the book is also an ideal reference for academic researchers and undergraduate and postgraduate students.