Arabidopsis Protocols

Author : Julio Salinas,Jose J. Sanchez-Serrano
Publisher : Springer Science & Business Media
Page : 461 pages
File Size : 49,5 Mb
Release : 2008-02-04
Category : Nature
ISBN : 9781597450034

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Arabidopsis Protocols, 2nd Edition by Julio Salinas,Jose J. Sanchez-Serrano Pdf

For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge.

Author : Jose J. Sanchez-Serrano,Julio Salinas
Publisher : Methods in Molecular Biology
Page : 754 pages
File Size : 50,7 Mb
Release : 2013-09-21
Category : Medical
ISBN : UCR:31210024230284

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Arabidopsis Protocols by Jose J. Sanchez-Serrano,Julio Salinas Pdf

Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome. These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future. In addition, considering the recent unparalleled progress of the “omics” tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Arabidopsis thaliana is acknowledged as the most important plant model system by the scientific community and Arabidopsis research has fundamentally influenced our understanding of the basic biology and ecology of plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Arabidopsis Protocols, Third Edition seeks to serve both experienced researchers and beginners with its detailed methodologies on this burgeoning scientific field.

Author : R. Paul Jarvis
Publisher : Unknown
Page : 128 pages
File Size : 48,6 Mb
Release : 2011
Category : Arabidopsis
ISBN : 1617792349

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Chloroplast Research in Arabidopsis by R. Paul Jarvis Pdf

Author : Kan Wang
Publisher : Springer Science & Business Media
Page : 474 pages
File Size : 52,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597451307

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Agrobacterium Protocols by Kan Wang Pdf

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Author : Nam-hai Chua,Csaba Koncz,Jeff Schell
Publisher : World Scientific Publishing Company
Page : 496 pages
File Size : 47,8 Mb
Release : 1992-06-12
Category : Electronic
ISBN : 9789813103429

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Methods In Arabidopsis Research by Nam-hai Chua,Csaba Koncz,Jeff Schell Pdf

One of the ways to make consistent progress in a particular field of biology consists in choosing a good model system on which to focus the experimental efforts of the scientific community. It has taken a long time for scientists interested in various aspects of the life of plants to reach some sort of consensus. With the advent and impact of molecular biology, the small weed Arabidopsis is now the object of rapidly growing scientific attention. Since it is reasonable to assume that the general molecular mechanisms that are responsible for the physiological, cellular and biochemical properties of plants will be essentially conserved in all plants, it follows that these mechanisms should also operate in Arabidopsis and hence that its genome should contain most of the genes that we need to know about if we want to understand the genetic determination of the life processes in plants.Arabidopsis has a small genome and well documented genetic studies are available. It is easy to grow in large numbers and mutants defining important genetically controlled mechanisms are either available, or can readily be obtained. Various methods to introduce and express isolated homologous or heterologous genes are available. It is therefore realistic and desirable to aim at exploring the genome of this plant in very great detail. As will be illustrated in this book all the elements for such a grand strategy are in place.More and more scientists are therefore willing to accept the obvious and very real practical disadvantages resulting from its small size when experiments call for the isolation of proteins, membranes, subcellular fractions etc, in order to benefit from its extraordinary experimental advantages as a model system in molecular genetics. One can safely predict that in the next decade studies with Arabidopsis will provide major breakthroughs in our understanding of most aspects of plant physiology and developmental biology. The importance of this knowledge for plant breeding and therefore for a sustainable highly productive agriculture cannot be overestimated. We therefore expect that this book will provide valuable guidelines to all those who are planning experiments aimed at understanding various aspects of plant growth, productivity and interactions with the environment. The book offers a wealth of methodical and theoretical information as well as valuable references. It should be of use to students, teachers, as well as advanced researchers and those breeders who want to use molecular techniques in breeding.

Author : Robert Carpentier
Publisher : Springer Science & Business Media
Page : 341 pages
File Size : 53,9 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592597994

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Photosynthesis Research Protocols by Robert Carpentier Pdf

Photosynthesis is one of the most important biological phenomena on earth. The conversion of sunlight by photosynthetic organisms supplies most of the energy required to develop and sustain life on the planet. Photosynthesis is not only at the heart of plant bioenergetics, it is also fundamental to plant prod- tivity and biomass. Photosynthetic carbon fixation and oxygen evolution - rectly intervene in many environmental, including the global atmospheric CO 2 level and global climate. Therefore, it is not surprising that a large effort is devoted to photosynthesis research. Several biochemical methods of isolation, treatment, and analysis have been developed to fulfill the needs of photosynthesis research. Photosynthesis Research Protocols contains a broad range of general and fundamental me- ods that are commonly used by plant biochemists, physiologists, and mole- lar biologists. This book is thus intended as a source of information for scientists working on any of the multiple aspects of photosynthesis, and should be of great interest to a multidisciplinary field of research involving agric- ture, biochemistry, biotechnology, botany, cell biology, environmental s- ences, forestry, plant genetics, plant molecular biology, photobiology, photophysics, photoprotection, plant physiology, plant stress, etc.

Author : R. Paul Jarvis
Publisher : Humana Press
Page : 432 pages
File Size : 40,8 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493962892

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Chloroplast Research in Arabidopsis by R. Paul Jarvis Pdf

Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable. The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid. In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

Author : Martin Bayer
Publisher : Unknown
Page : 298 pages
File Size : 44,5 Mb
Release : 2020
Category : Botany
ISBN : 1071603426

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Plant Embryogenesis by Martin Bayer Pdf

This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species. Chapters guide readers through genetic screens, phenotypic analysis, live imaging, transcriptional profiling, methods on other model and non-model species beyond Arabidopsis thaliana, and introduction to systems that allow to culture or produce zygotic and somatic embryos in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Embryogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Author : Gregory A. Tucker,Jeremy A. Roberts
Publisher : Springer Science & Business Media
Page : 201 pages
File Size : 45,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592590674

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Plant Hormone Protocols by Gregory A. Tucker,Jeremy A. Roberts Pdf

Established investigators from around the world describe in step-by-step detail their best techniques for the study of plant hormones and their regulatory activities. These state-of-the-art methods include contemporary approaches to identifying the biosynthetic pathways of plant hormones, monitoring their levels, characterizing the receptors with which they interact, and analyzing the signaling systems by which they exert their effects. Comprehensive and fully detailed for reproducible laboratory success, Plant Hormone Protocols offers plant biologists an indispensable compendium of today's most powerful methods and strategies to studying plant hormones, their regulation, and their activities.

Author : Edward Chee Tak Yeung,Claudio Stasolla,Michael John Sumner,Bing Quan Huang
Publisher : Springer
Page : 576 pages
File Size : 50,5 Mb
Release : 2015-09-19
Category : Science
ISBN : 9783319199443

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Plant Microtechniques and Protocols by Edward Chee Tak Yeung,Claudio Stasolla,Michael John Sumner,Bing Quan Huang Pdf

A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.​

Author : Ive De Smet
Publisher : Humana Press
Page : 356 pages
File Size : 45,5 Mb
Release : 2013-01-09
Category : Science
ISBN : 1627032207

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Plant Organogenesis by Ive De Smet Pdf

Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these processes take place embedded deep in tissues or are difficult to access or visualize. To achieve this, we need specialized techniques such as those concisely illustrated in Plant Organogenesis: Methods and Protocols. Chapters address topics such as how to study and image the structure of ovules and embryos of Arabidopsis thaliana, tools to establish cell lineages in order to visualize the contribution of each cell and cell division to the building of a mature organ, approaches to study the totipotency of several plant cells, techniques such as the use of fluorescence-activated cell sorting (FACS) to analyse transcriptomes and hormone levels in Arabidopsis, methods to investigate organogenesis in economically important crops, and computer-based approaches to bring everything together. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Organogenesis: Methods and Protocols serves both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this fascinating research field.

Author : David Edwards
Publisher : Springer Science & Business Media
Page : 551 pages
File Size : 51,8 Mb
Release : 2008-01-07
Category : Science
ISBN : 9781588296535

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Plant Bioinformatics by David Edwards Pdf

This book aims to assist research scientists in choosing the most applicable database or bioinformatics tools to aid and promote their research in plant biotechnology. Chapters include practical examples and highlight common problems encountered in bioinformatics analysis. Further chapters are aimed at researchers developing bioinformatics databases and tools, detailing commonly applied database formats and biology-focused scripting languages.

Author : Daniela Ristova,Elke Barbez
Publisher : Humana
Page : 324 pages
File Size : 46,5 Mb
Release : 2019-03-25
Category : Science
ISBN : 1493992732

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Root Development by Daniela Ristova,Elke Barbez Pdf

This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental methods for quantification of different root developmental processes to complex methods that require sophisticated equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Root Development: Methods and Protocols serves as a valuable tool for plant biologists specialized in root development, from beginners to experienced researchers in the field.

Author : John Flex Jackson,Hans F. Linskens
Publisher : Springer Science & Business Media
Page : 194 pages
File Size : 48,6 Mb
Release : 2013-03-09
Category : Science
ISBN : 9783662049044

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Testing for Genetic Manipulation in Plants by John Flex Jackson,Hans F. Linskens Pdf

Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy sis have now decided to rename the series Molecular Methods of Plant Analy sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not mean that all chapters a priori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen recent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis. As indicated for previous volumes, the methods to be found in Molecular Methods of Plant Analysis are described critically, with hints as to their limi tations, references to original papers and authors being given, and the chap ters written so that there is little need to consult other texts to carry out the All authors have been chosen because of their methods of analysis described.

Author : Knox Van Dyke,Christopher Van Dyke,Karen Woodfork
Publisher : CRC Press
Page : 621 pages
File Size : 51,7 Mb
Release : 2001-12-20
Category : Science
ISBN : 9781420041804

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Luminescence Biotechnology by Knox Van Dyke,Christopher Van Dyke,Karen Woodfork Pdf

The dangers and drawbacks inherent in radioactivity-based methods along with a demonstrated and dramatic increase in sensitivity have precipitated a major shift towards luminescence measurements and visualization techniques. Their use has now spread even to traditional clinical environments, and their applications have grown from clinical assays to