Arabidopsis Protocols 2nd Edition

Author : Julio Salinas,Jose J. Sanchez-Serrano
Publisher : Springer Science & Business Media
Page : 461 pages
File Size : 49,6 Mb
Release : 2008-02-04
Category : Nature
ISBN : 9781597450034

Get Book

Arabidopsis Protocols, 2nd Edition by Julio Salinas,Jose J. Sanchez-Serrano Pdf

For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge.

Author : José M. Martínez-Zapater,Julio Salinas
Publisher : Springer Science & Business Media
Page : 440 pages
File Size : 40,7 Mb
Release : 1998-01
Category : Nature
ISBN : 0896033910

Get Book

Arabidopsis Protocols by José M. Martínez-Zapater,Julio Salinas Pdf

This comprehensive collection of current and essential protocols contains many easily reproducible methods developed for use with Arabidopsis - a system for approaching fundamental questions in plant biology. The methods range from the basics of growing these plants to sophisticated gene cloning strategies and can, in many cases, also be applied to other plant species with minor modifications. Sections on genetics, transformation and gene expression analysis that are especially helpful to scientists involved in mutant analysis or producing and analyzing transgenic plants.

Author : R. Paul Jarvis
Publisher : Unknown
Page : 128 pages
File Size : 54,5 Mb
Release : 2011
Category : Arabidopsis
ISBN : 1617792349

Get Book

Chloroplast Research in Arabidopsis by R. Paul Jarvis Pdf

Author : Hydar Ali,Haribabu Bodduluri
Publisher : Springer Science & Business Media
Page : 354 pages
File Size : 52,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781597450485

Get Book

Transmembrane Signaling Protocols by Hydar Ali,Haribabu Bodduluri Pdf

The previous edition of Transmembrane Signaling Protocols was published in 1998. Since then the human genome has been completely sequenced and new methods have been developed for the use of microarrays and proteomics to analyze global changes in gene expression and protein profiles. These advances have increased our ability to understand transmembrane signaling processes in much greater detail. They have also simultaneously enhanced our ability to determine the role of a large number of newly identified molecules in signaling events. In addition, novel video microscopy methods have been developed to image transmembrane signaling events in live cells in real time. In view of these major advances, it is time to update the previous edition. Because of the success of that volume, we have chosen to keep the essential character of the book intact. Introductory chapters from experts have been included to provide overall perspective and an overview of recent advances in signal transduction pathways. The individual chapters now include comp- hensive detailed methods, studies in genetically tractable systems, fluorescence microscopy in live single cells, ex vivo analysis of primary cells from tra- genic mice, as well as genomic and proteomic approaches to the analysis of transmembrane signaling events. We would like to express our deep gratitude to the coauthors of this publi- tion. We hope that Transmembrane Signaling Protocols, Second Edition will serve as a valuable resource for future progress in the study of signal transd- tion pathways.

Author : Kursad Turksen
Publisher : Springer Science & Business Media
Page : 504 pages
File Size : 48,6 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450379

Get Book

Embryonic Stem Cell Protocols by Kursad Turksen Pdf

Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

Author : Louise O’Connor
Publisher : Springer Science & Business Media
Page : 226 pages
File Size : 53,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781597451437

Get Book

Diagnostic Bacteriology Protocols by Louise O’Connor Pdf

The field of bacterial diagnostics has seen unprecedented advances in recent years. The increased need for accurate detection and identification of bacteria in human, animal, food, and environmental samples has fueled the development of new techniques. The field has seen extensive research aided by the information from bacterial genome sequencing projects. Although traditional methods of bacterial detection and identification remain in use in laboratories around the world, there is now a growing trend toward the use of nucleic ac- based diagnostics and alternative biochemically and immunologically based formats. The ultimate goal of all diagnostic tests is the accurate detection, identification, or typing of microorganisms in samples of interest. Although the resulting information is of obvious use in the areas of patient management, animal health, and quality control, it is also of use in monitoring routes of infection and outlining strategies for infection control. There is, therefore, a need to ensure that the information being provided is of the highest standard and that any new technique is capable of delivering this.

Author : Ian R. Phillips
Publisher : Springer Science & Business Media
Page : 355 pages
File Size : 51,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592599981

Get Book

Cytochrome P450 Protocols by Ian R. Phillips Pdf

For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.

Author : Shao-Yao Ying
Publisher : Springer Science & Business Media
Page : 365 pages
File Size : 44,8 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597451239

Get Book

MicroRNA Protocols by Shao-Yao Ying Pdf

MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.

Author : KURSAD TURKSEN
Publisher : Springer Science & Business Media
Page : 390 pages
File Size : 43,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450461

Get Book

Human Embryonic Stem Cell Protocols by KURSAD TURKSEN Pdf

A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Author : Daryl S. Henderson
Publisher : Springer Science & Business Media
Page : 498 pages
File Size : 40,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592599738

Get Book

DNA Repair Protocols by Daryl S. Henderson Pdf

The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to expedite editing and prod- tion of this volume. Although DNA Repair Protocols: Mammalian Systems is a smaller book than its predecessor, it actually contains a greater variety of methods. Fourteen of the book’s thirty-two chapters are entirely new and areas of redundancy present in the first edition have been eliminated here (for example, now just two chapters describe assays for nucleotide excision repair [NER], rather than seven). All eighteen returning chapters have been revised, many of them ext- sively. In order to maintain a coherent arrangement of topics, the four-part p- titioning seen in the first edition was dispensed with and chapters concerned with ionizing radiation damage and DNA strand breakage and repair were re- cated to near the front of the book. Finally, an abstract now heads each chapter.

Author : Peng Liang,Jonathan Meade,Arthur B. Pardee
Publisher : Springer Science & Business Media
Page : 321 pages
File Size : 44,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592599684

Get Book

Differential Display Methods and Protocols by Peng Liang,Jonathan Meade,Arthur B. Pardee Pdf

Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an “open”-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms—from bacteria, fungi, insects, fish, plants, to mammals—even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious “cottage-industry” academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.

Author : Wei Xiao
Publisher : Springer Science & Business Media
Page : 389 pages
File Size : 52,8 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592599585

Get Book

Yeast Protocols by Wei Xiao Pdf

In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.

Author : X. Johné Liu
Publisher : Springer Science & Business Media
Page : 487 pages
File Size : 52,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781597450003

Get Book

Xenopus Protocols by X. Johné Liu Pdf

A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance, its striking protein translation capacity, and its breathtaking possibilities for the assembly of infectious viral particles by single cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction, and cover all the major uses of oocytes/extracts as experimental models.

Author : Philip Hornick
Publisher : Springer Science & Business Media
Page : 430 pages
File Size : 44,6 Mb
Release : 2008-02-05
Category : Medical
ISBN : 9781597450492

Get Book

Transplantation Immunology by Philip Hornick Pdf

Leading clinicians and scientists in solid organ transplantation review the current status of the field and describe cutting-edge techniques for detecting the immune response to the allografted organ. The authors present the latest techniques for HLA typing, detecting HLA antibodies, and monitoring T-cell response, and examine more specialized methods utilizing proteomics, laser dissection microscopy, and real-time polymerase chain reaction. The area of tolerance induction and reprogramming of the immune system is also covered, along with a discussion of up-to-date methods of organ preservation, of today's optimal immunosuppressive drug regimens, as well as the difficulty of mimicking chronic rejection in experimental models. Introductory chapters provide a theoretical update on current practices in renal, liver, islet, and lung transplantation and on the pathways of antigen presentation and chronic rejection.

Author : James D. Stockand,Mark S. Shapiro
Publisher : Springer Science & Business Media
Page : 208 pages
File Size : 54,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450959

Get Book

Ion Channels by James D. Stockand,Mark S. Shapiro Pdf

The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation.