Assay Of Protein And Polypeptide Hormones

Author : H. Van Cauwenberge,P. Franchimont
Publisher : Elsevier
Page : 255 pages
File Size : 53,9 Mb
Release : 2014-05-17
Category : Medical
ISBN : 9781483145969

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Assay of Protein and Polypeptide Hormones by H. Van Cauwenberge,P. Franchimont Pdf

International Series of Monographs in Pure and Applied Biology, Modern Trends in Physiological Sciences, Volume 33: Assay of Protein and Polypeptide Hormones focuses on the assays of protein and polypeptide hormones. The selection first discusses the general principles of protein and polypeptide hormone assays, including conditions and basic factors for bioassays and status of biological assays. The book looks at the assays of hypophyseal hormones, such as growth hormones, adrenocorticotropin (ACTH), thyrotropin, gonadotropins, prolactin, vasopressin, and oxytocin. Concerns include radioimmunoassay, standards of thyrotropin, immunoassay of hypophyseal gonadotropins, and bioassay of prolactin. The book evaluates the assays of pancreatic hormones, such as insulin and glucagon. Chemical structure and properties of insulin and radioimmunoassay of glucagon are explained. The text also focuses on the assays of erythropoietin, renin, human chorionic gonadotropin, and human placental lactogen (HPL). Concerns include augmentation of red cell volume; biological application of renin assays; and similarities and differences between hypophyseal somatotropin and HPL. The selection is a valuable source of data for readers interested in the assays of protein and polypeptide hormones.

Author : M. Margoulies,Frederick C. Greenwood
Publisher : Unknown
Page : 600 pages
File Size : 46,6 Mb
Release : 1971
Category : Drugs
ISBN : CORNELL:31924000916365

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Structure-activity Relationships of Protein and Polypeptide Hormones by M. Margoulies,Frederick C. Greenwood Pdf

Author : Ruth Porter,David W. FitzSimons
Publisher : John Wiley & Sons
Page : 192 pages
File Size : 47,7 Mb
Release : 2009-09-16
Category : Science
ISBN : 9780470717912

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Polypeptide Hormones by Ruth Porter,David W. FitzSimons Pdf

The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.

Author : Dr. Wolfgang König
Publisher : Unknown
Page : 304 pages
File Size : 47,7 Mb
Release : 1993
Category : Medical
ISBN : UOM:39015029972844

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Peptide and Protein Hormones by Dr. Wolfgang König Pdf

Incorporates the developments in the field during the 25 years since the last major overview of peptide and protein hormones. Reviews the most important features of the hormones, including structure/activity relationships. Also describes their medical use, noting that western society encourages an overabundance of aggressive stress hormones, and that the role of doctors is to return them into balance with cooperative sex hormones. Of particular use to researchers, students, and practitioners is a special listing of the hormones according to a network of structural similarity and regulating cascades. Annotation copyright by Book News, Inc., Portland, OR

Author : Nathan Back
Publisher : Springer
Page : 671 pages
File Size : 50,7 Mb
Release : 2013-12-11
Category : Medical
ISBN : 9781461446125

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Pharmacology of Hormonal Polypeptides and Proteins by Nathan Back Pdf

It can be concluded (under the specific experimental procedures em ployed) that:- 1) HCG labelled with 1 - 2 atoms of radioactive iodine did not differ sig nificantly from the unlabelled hormone; 2) The ovary alone exhibited a capacity to affix specifically HCG; 3) The amount of radioactive material in the ovary was directly proport ional to the quantity of labelled HCG injected; 4) When the HCG present in the circulation is bound to an antiserum to HCG, the antigen-antibody complex is not concentrated by the ovary; 5) Circulating labelled HCG decreased to 50% within 30 minutes following a single intravenous injection; 6) There are four different phases of ovarian uptake of HCG, namely: the first phase, when there is only an inflow from the circulation and stor age mainly in the follicular envelopes; the second phase, when there is a greater inflow than outflow; the third phase, when the inflow is equal to the outflow; and the fourth period, when the outflow is bigger than the in flow. REFERENCES 1. Lunenfeld, B. and Eshkol, A. Vitamins and Hormones (1967) 25:165 2. Eshkol, A. In: Recent Research on Gonadotropbio Hormones, eds. E. T. Bell andJ. A. Loraine, Edinburgh, Livingstone (1967), p. 202. 3. Eshkol, A. and Lunenfeld, B. Proc. Tel-Hashomer Hosp. (1967) 6:4. ACKNOWLEOOMEN'IS This work was supported in part by a grant from the Population Council, N. Y. , U. S. A. andbyGrantNo.

Author : John E. Shively
Publisher : Springer Science & Business Media
Page : 458 pages
File Size : 44,6 Mb
Release : 2008-02-22
Category : Science
ISBN : 9781592594368

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Methods of Protein Microcharacterization by John E. Shively Pdf

Milestones in the techniques and methodology of polypeptide structure determination include the determination of the sequence of insulin by Sanger in 1951 (I) and the introduction of the repeti tive degradation of proteins with phenylisothiocyanate by Edman in 1959 (2). The automation of Edman chemistry (3) played a major role in the determination of polypeptide structures. Important modifications of Edman chemistry include the solid-phase approach by Laursen in 1971 (4) and the use of modified Edman reagents such as 4-N, N-dimethylaminoazobenzene-4'-isothiocy- ate (DABITC) for manual sequencing by Chang et al. (5) in 1976. A second major breakthrough in the analysis of polypeptides was automated amino acid analysis described by Spackman et al. in 1958 (6). However, during the period from 1975 to 1980, it became increasingly clear that the amount of material required for struc tural analysis was more than could be easily isolated for the vast majority of proteins. The field was criticized for its lack of sensitive techniques for the analysis of growth factors, immune modulators, membrane receptors, and peptide hormones. In addition, very little had been done to modernize and improve the original instruments introduced in the mid-1960s. The first indications of improved instrumentation for Edman chemistry came from Wittmann-Liebold's laboratory (7), followed by the introduction of a "micro" sequencer by Hunkapiller and Hood in 1978 (8). The movement toward improved instrumentation culminated in the "gas"--Phase sequencer of Hewick et al. (9) in 1981

Author : John G. Pierce
Publisher : Unknown
Page : 458 pages
File Size : 48,6 Mb
Release : 1982
Category : Gastrointestinal hormones
ISBN : 0879334177

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Protein and Peptide Hormones by John G. Pierce Pdf

Author : Filippo Polvani
Publisher : Elsevier
Page : 274 pages
File Size : 44,5 Mb
Release : 2012-12-02
Category : Medical
ISBN : 9780323158213

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Immunological Properties of Protein Hormones by Filippo Polvani Pdf

Immunological Properties of Protein Hormones covers the proceedings of the 1964 International Endocrinological Symposia on Immunological Properties of Protein Hormones, held in Rome, Italy. This symposium is organized under the auspices of Accademia Nazionale dei Lincei and of Consiglio Nazionale delle Rieerche Rome. This book is organized into four sections encompassing 27 chapters that tackle the immunological properties of gonadotropins, growth hormones, and insulin. The first section presents first an overview of immunological studies of protein hormones, followed by a discussion on the application of immunofluorescence to localization of pituitary hormones. This section also looks into the antigenic property of ACTH antibodies. The remaining sections cover the immunoassay methods of analysis, occurrence, distribution, clinical and physiological aspects, functions, and antigenic properties of the specific protein hormones. This book is of great benefit to immunoendocrinologists.

Author : Andreas Schaller
Publisher : Springer Nature
Page : 312 pages
File Size : 45,8 Mb
Release : 2023-11-29
Category : Science
ISBN : 9781071635117

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Plant Peptide Hormones and Growth Factors by Andreas Schaller Pdf

This book describes state-of-the-art approaches for identification and functional characterization of plant peptide hormones and growth factors. Chapters guide readers through protocols for computational identification of novel signaling peptides and phytocytokines, analysis of post-translational maturation events, isolation of mature peptides from plant tissues, peptide bioassays, in vivo and in vitro analysis of peptide activity, peptide receptor identification and interaction analysis, and quantitative assessment of peptide ligand binding to cognate receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Peptide Hormones and Growth Factors aims to be a useful and practical guide to new researchers and experts looking to expand their technical know-how.

Author : J. Chayen
Publisher : Springer
Page : 232 pages
File Size : 53,5 Mb
Release : 1980-08
Category : Medical
ISBN : UCAL:B4300052

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The Cytochemical Bioassay of Polypeptide Hormones by J. Chayen Pdf

The cytochemical bioassay system was described in a short abstract in 1971, and more fully, in the cytochemical bioassay of corticotrophin, in 1972. Since then, cytochemical bioassays have been described for several polypeptide hormones, and these assays are already widely used. It is expedient that the subject should be reviewed, as it is in this monograph, by one writer who has had the good fortune to have taken part in the growth of cytochemistry from its early origins to its present position as the basis of possibly the most sensitive bioassay system currently available. However, it should be noted that major contributions have been made by many, both to the development of the subject and to the establishment of the bioassays. The object of this preface is to try to give some perspective to the growth of this subject and to record that the cytochemical bioassay system has been fostered by many outstanding scientists in an atmosphere of remarkable goodwill. To begin with, there could have been no cytochemical bioassays until cytochem istry had been converted from its rather unsure origins into a precise and quantitative form of cellular biochemistry. This was done with skill and enthusiastic dedication by my colleagues, Dr. Lucille Bitensky, Dr. F. P. Altman, Dr. R. G. L. W. Poulter and Mr. A. A. Silcox, first at the Royal College of Butcher, Dr.

Author : Anonim
Publisher : Unknown
Page : 604 pages
File Size : 49,9 Mb
Release : 1971
Category : Drugs
ISBN : WISC:89030565345

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Structure-activity Relationships of Protein and Polypeptide Hormones by Anonim Pdf

Author : K. Siddle,J. C. Hutton
Publisher : Oxford University Press, USA
Page : 376 pages
File Size : 50,6 Mb
Release : 1990
Category : Medical
ISBN : UOM:39015058999437

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Peptide Hormone Secretion by K. Siddle,J. C. Hutton Pdf

Polypeptide hormones play a central role in the regulation of biological processes from the minute-to-minute control of vital functions to the programming of birth, development, reproduction, and ageing. The first of a two-volume work on the methods of study used in this field, Peptide Hormone Secretion focuses on the isolation of polypeptide hormones and their identification and structural analysis, the development of sensitive bioassay and immunoassay techniques, and tissue localization of peptide hormones and their encoding MRNAs, as well as techniques for characterization of peptide hormone receptors. Ideal for graduate students and researchers, the book includes coverage of the general principles and strategies that the methods are based on, so the techniques will have a wide application to biologically active peptides involved in neurotransmission, growth regulations, differentiation, and vasoregulation.

Author : M. Wallis,S. L. Howell,Keith William Taylor
Publisher : John Wiley & Sons
Page : 512 pages
File Size : 49,5 Mb
Release : 1985
Category : Nature
ISBN : UOM:39015009572275

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The Biochemistry of the Polypeptide Hormones by M. Wallis,S. L. Howell,Keith William Taylor Pdf

The Biochemistry of Polypeptide Hormones is intended to meet the needs particularly of advanced undergraduates, preclinical students and postgraduates, and provide an introduction to the research literature. All the main groups of polypeptide hormones are covered, and special chapters deal with structure-function relationships, hormone receptors, the role of second messengers and the applications of recombinant DNA technology.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 55,9 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Bernd Gutte
Publisher : Elsevier
Page : 527 pages
File Size : 47,6 Mb
Release : 1995-10-24
Category : Science
ISBN : 9780080538594

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Peptides by Bernd Gutte Pdf

In recent years, research has shown the importance of peptides in neuroscience, immunology, and cell biology. Active research programs worldwide are now engaged in developing peptide-based drugs and vaccines using modification of natural peptides and proteins, design of artificial peptides and peptide mimetics, and screening of peptide and phage libraries. In this comprehensive book, the authors discuss peptide synthesis and application within the context of their increasing importance to the pharmaceutical industry. Peptides: Synthesis, Structures, and Applications explores the broad growth of information in modern peptide synthetic methods and the structure-activity relationships of synthetic polypeptides. The history of peptide chemistry Amide formation, deprotection, and disulfide formation in peptide synthesis Solid-phase peptide synthesis a-helix formation by peptides in water Stability and dynamics of peptide conformation An overview of structure-function studies of peptide hormones Neuropeptides:peptide and nonpeptide analogs Reversible inhibitors of serine proteinases Design of polypeptides Current capabilities and future possibilities of soluble chemical combinatorial libraries Epitope mapping with peptides Synthesis and applications of branched peptides in immunological methods and vaccines