Baculovirus Expression Vectors

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Baculovirus Expression Vectors

David R. O'Reilly,Lois K. Miller,Verne A. Luckow
Baculovirus Expression Vectors Book PDF

Author : David R. O'Reilly,Lois K. Miller,Verne A. Luckow
Publisher : Unknown
Page : 366 pages
File Size : 49,7 Mb
Release : 1994
Category : Baculoviruses
ISBN : 0195091310

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Baculovirus Expression Vectors by David R. O'Reilly,Lois K. Miller,Verne A. Luckow Pdf

Baculoviruses have proven to be the most powerful and versatile eukaryotic expression vectors available. This unique laboratory manual is designed to help both beginning and experienced researchers construct and use baculovirus vector systems. It simplifies selection of the most appropriatebaculovirus vector design for a given problem, then describes each step of the implementation process--from vector construction to large-scale protein production. The book provides an understanding of how the vectors work; a biological overview of cells, viruses, plasmids, and promoters; guidelinesfor choosing optimum vectors; protocols for growing insect cells and recombinant viruses; methods of analyzing protein products and scaling up protein production; techniques for producing proteins in insect larvae; and easy-to-use maps charting available expression vectors. This comprehensiveapproach has many benefits for researchers and students alike. It allows them to understand how and why the vector system works and offers a rapid comparison of options for choosing the right virus, plasmid or promoter for vector design and construction, with a minimum amount of lost time. Themanual is an invaluable resource for every individual engaged in the production of proteins for any purpose.

Baculovirus Expression Protocols

Christopher D. Richardson
Baculovirus Expression Protocols Book PDF

Author : Christopher D. Richardson
Publisher : Springer Science & Business Media
Page : 412 pages
File Size : 55,5 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595266

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Baculovirus Expression Protocols by Christopher D. Richardson Pdf

Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors-leading authorities in the field-present the assorted expression plasmids currently in use, guide the reader through the process of generating and selecting recombinant virus, and describe specific examples of recombinant protein production and purification. The emphasis is on alternative and simpler screening techniques for the selection of recombinant baculovirus. The book also surveys the various insect cell lines currently compatible with the baculovirus system. Highlights include production of recombinant virus using linearized DNA and vectors that contain a b-galactosidase indicator and a complete list of expression vectors currently available. Detailed descriptions for the scale-up of protein production using spinner flasks, bioreactors, and insect larvae are also included. Baculovirus Expression Protocols offers both industrial and university-based researchers an outstanding collection of reproducible, step-by-step laboratory protocols. It will immediately become indispensable for anyone working with baculoviruses and their application in the expression of recombinant proteins in insect cells.

Baculovirus and Insect Cell Expression Protocols

David W. Murhammer
Baculovirus and Insect Cell Expression Protocols Book PDF

Author : David W. Murhammer
Publisher : Methods in Molecular Biology
Page : 522 pages
File Size : 46,5 Mb
Release : 2018-04-15
Category : Medical
ISBN : 1493979787

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Baculovirus and Insect Cell Expression Protocols by David W. Murhammer Pdf

The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

The Baculovirus Expression System

Linda King
The Baculovirus Expression System Book PDF

Author : Linda King
Publisher : Springer Science & Business Media
Page : 242 pages
File Size : 52,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401123747

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The Baculovirus Expression System by Linda King Pdf

The decision to write a book about the practical aspects of the baculovirus expression system stems from the numerous phone calls for help we have had, and from the many visitors to our labora tories requiring assistance to find the elusive polyhedrin-negative virus containing their favourite gene. We have also organized two expression system workshops and from the manuals we wrote for these, it seemed a logical progression to extend them into book form. We appreciate that those who are 'old-hands' at the baculovirus expression system may have differing views on some of our procedures, but the methods in this book are presented in the light of our own experiences in the laboratory and from our practical workshops, and we hope that the book will be especially useful to those new to the system. The first three chapters give the background information to the baculovirus expression system, and includes advice on how to choose the right transfer vector and discusses the various methods that are available to select recombinant viruses. The practical chapters concentrate on those aspects which are novel to the baculovirus system (insect cell culture, virus amplification and titration, etc. ) and, in general, leave the standard molecular biological techniques to the other excellent laboratory manuals that are available. However, for completeness sake and to avoid constant reference to other manuals, we have included brief details of some standard techniques where they are integral to the success of the baculovirus protocols.

The Baculoviruses

Lois K. Miller
The Baculoviruses Book PDF

Author : Lois K. Miller
Publisher : Springer Science & Business Media
Page : 459 pages
File Size : 40,9 Mb
Release : 2013-06-29
Category : Medical
ISBN : 9781489918345

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The Baculoviruses by Lois K. Miller Pdf

The past decade has witnessed an explosion of information on the molecular biology of insect viruses and a frenzy of activity in applying this information to medicine and agriculture. Genetically engineered baculoviruses are presently being tested for commercial use as pesticides, and the study of such viruses is also revealing remarkable insights into basic cellular processes such as apoptosis. This comprehensive volume provides readers with knowledge of basic and applied baculovirology so that current literature in the field can be appreciated.

Viral Expression Vectors

Nicholas Muzyczka
Viral Expression Vectors Book PDF

Author : Nicholas Muzyczka
Publisher : Springer Science & Business Media
Page : 183 pages
File Size : 46,8 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642756085

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Viral Expression Vectors by Nicholas Muzyczka Pdf

In the past ten years there has been enormous progress in the development of eukaryotic viral vectors. In general, these vectors have been developed for one of three reasons: to achieve high levels of expression of a particular gene product (poxvirus, baculovirus, and adenovirus), to clone eukaryotic genes in combination with functional assays (Epstein-Barr virus), of for use as delivery vehicles for the stable introduction of foreign genes into mammalian cells (retroviruses, Epstein-Barr virus, and adeno-associated virus). Each vector has its strengths and weaknesses that are rooted in the sometimes bewildering stra tegies that the parent viruses use for propagation. No one of these vectors is appropriate for all of the problems that a mole cular biology laboratory is likely to encounter, and few of us are knowledgeable in the molecular virology of all of these viruses. This volume represents an attempt by the authors to assem ble a review of these vectors in one place and in a form useful to laboratories that do not necessarily have experience with eukaryotic viruses. Clearly, any virus can be modified to serve as a vector for some purposes, and it was not possible to include a description of all of these. In addition, one eukaryotic vector, SV40 (the first one developed), has been reviewed so widely that we saw no reason to include it here.

Gene Expression Systems

Joseph M. Fernandez,James P. Hoeffler
Gene Expression Systems Book PDF

Author : Joseph M. Fernandez,James P. Hoeffler
Publisher : Elsevier
Page : 493 pages
File Size : 40,5 Mb
Release : 1998-12-21
Category : Science
ISBN : 9780080532356

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Gene Expression Systems by Joseph M. Fernandez,James P. Hoeffler Pdf

Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Provides detailed information on expression systems Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs Includes timelines and examples Compares pros and cons of each method

Expression Systems

Michael Dyson,Yves Durocher
Expression Systems Book PDF

Author : Michael Dyson,Yves Durocher
Publisher : Scion Publishing Ltd
Page : 311 pages
File Size : 40,9 Mb
Release : 2007-09-15
Category : Science
ISBN : 9781907904400

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Expression Systems by Michael Dyson,Yves Durocher Pdf

1. Expression strategy (Michael Dyson) 2. Protein expression in Escherichia coli (Rosalind Kim) 3. Expression engineering of synthetic antibodies using ribosome display (Matthew DeLisa and Lydia M. Contreras Martinez) 4. Refolding proteins from inclusion bodies (Renaud Vincentelli) 5. Selection of protein variants with improved expression using GFP-derived folding and solubility reporters (Geoffrey Waldo and Stéphanie Cabantous) 6. Protein expression in the wheat germ cell-free system (Yaeta Endo and Tatsuya Sawasaki) 7. Saccharomyces cerevisiae ; A microbial eukaryotic expression system (Christine Lang) 8. Expression of proteins in Pichia pastoris (Geoff and Joan Lin-Cereghino and Wilson Leung) 9. Improved baculovirus expression vectors (Linda King, Richard Hitchman and Robert Possee) 10. Transient transfection of insect cells for rapid expression screening and protein production (Robert Novy et al.) 11. Generation of stable CHO cell lines for protein expression (Zhijian Lu et al.) 12. Transient expression in HEK293-EBNA1 cells (Yves Durocher, Roseanne Tom and Louis Bisson) 13. Nisin- and subtilin-controlled gene expression systems for Gram-positive bacteria (Oscar Kuipers and Jan Kok) 14. Protein expression using lentiviral vectors (Bernard Massie, Renald Gilbert and Sophie Broussau) 15. Expression in mammalian cells using BacMam viruses (Yu-Chen Hu and Hsiao-Ping Lee) List of suppliers;Index

Advanced Technologies for Protein Complex Production and Characterization

M. Cristina Vega
Advanced Technologies for Protein Complex Production and Characterization Book PDF

Author : M. Cristina Vega
Publisher : Springer
Page : 382 pages
File Size : 45,8 Mb
Release : 2016-05-10
Category : Science
ISBN : 9783319272160

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Advanced Technologies for Protein Complex Production and Characterization by M. Cristina Vega Pdf

This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.

Insect Cell Culture Engineering

Mattheus F. A. Goosen
Insect Cell Culture Engineering Book PDF

Author : Mattheus F. A. Goosen
Publisher : CRC Press
Page : 260 pages
File Size : 41,8 Mb
Release : 2020-07-24
Category : Science
ISBN : 9781000105667

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Insect Cell Culture Engineering by Mattheus F. A. Goosen Pdf

Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.

Trends in Insect Molecular Biology and Biotechnology

Dhiraj Kumar,Chengliang Gong
Trends in Insect Molecular Biology and Biotechnology Book PDF

Author : Dhiraj Kumar,Chengliang Gong
Publisher : Springer
Page : 378 pages
File Size : 53,5 Mb
Release : 2018-04-03
Category : Science
ISBN : 9783319613437

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Trends in Insect Molecular Biology and Biotechnology by Dhiraj Kumar,Chengliang Gong Pdf

This book provides an overview on the basics in insect molecular biology and presents the most recent developments in several fields such as insect genomics and proteomics, insect pathology and applications of insect derived compounds in modern research. The book aims to provide a common platform for the molecular entomologist to stimulate further research in insect molecular biology and biotechnology. Insects are one of the most versatile groups of the animal kingdom. Due to their large population sizes and adaptability since long they attract researchers’ interest as efficient resource for agricultural and biotechnological purposes. Several economically important insects such as Silkworms, Honey Bee, Lac and Drosophila or Termites were established as invertebrate model organisms. Starting with the era of genetic engineering, a broad range of molecular and genetic tools have been developed to study the molecular biology of these insects in detail and thus opened up a new horizon for multidisciplinary research. Nowadays, insect derived products are widely used in biomedical and biotechnology industries. The book targets researchers from both academia and industry, professors and graduate students working in molecular biology, biotechnology and entomology.

Baculovirus Expression System

Linda King,R.D. Possee
Baculovirus Expression System Book PDF

Author : Linda King,R.D. Possee
Publisher : Springer
Page : 304 pages
File Size : 49,8 Mb
Release : 1997-07-31
Category : Technology & Engineering
ISBN : 0412755505

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Baculovirus Expression System by Linda King,R.D. Possee Pdf

This is a thoroughly updated new edition which takes into account all of the many recent advances in the baculovirus expression vector system, now one of the most popular eukaryotic expression systems used. This manual is aimed at any researchers using the system in academic or industrial laboratories, and can be used by those new to the technique as well as those experienced with these methods.

Insect Viruses

Byrony C Bonning
Insect Viruses Book PDF

Author : Byrony C Bonning
Publisher : Academic Press
Page : 513 pages
File Size : 49,8 Mb
Release : 2006-09-22
Category : Science
ISBN : 9780120398683

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Insect Viruses by Byrony C Bonning Pdf

Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management purposes, but also as laboratory research tools for production of recombinant proteins and for protein display, and as potential vectors for human gene therapy. In addition to highlighting recent advances, this volume provides a comprehensive review of the biotechnological applications of these and other insect viruses in both the academic and private sectors.

Cell Engineering

Mohamed Al-Rubeai
Cell Engineering Book PDF

Author : Mohamed Al-Rubeai
Publisher : Springer
Page : 223 pages
File Size : 51,6 Mb
Release : 2012-03-01
Category : Science
ISBN : 9401143161

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Cell Engineering by Mohamed Al-Rubeai Pdf

The advantages of the baculovirus system are rooted in the properties of the virus and the host (insect, or cell lines derived from it). During the normal infection cycle, two forms of the virus are produced: an early budded virus (BY) form (Kost et al. , 2000), in which the viral DNA and structural proteins are surrounded by membrane derived from the infected cell; and a late occluded form (occlusion-derived virus, ODy), consisting of enveloped viral cores which are embedded in a crystal matrix of viral proteins. The principal component of the matrix is the abundantly expressed protein polyhedrin. The budded virus rapidly spreads the infection from cell to cell within the insect host, resulting ultimately in the complete liquefaction of the host, and release of occluded virus into the environment. The occluded form protects the released virus, allowing it to survive for long periods in the environment until ingested by another host. In the alkaline environment ofthe insect gut, the protective protein matrix is removed, and the life cycle is repeated. In insect cell cultures, only the BV form of baculovirus is required, and the polyhedrin gene may be replaced with the gene for the recombinant protein. An additional benefit of replacing or deleting polyhedrin is that it effectively makes the virus unable to survive outside the laboratory, an advantage in terms of environmental safety. The system is intrinsically safe to animals, being unable to replicate in species other than a limited range of insects.

Viral Vectors for Gene Therapy

Fredric P. Manfredsson,Matthew J. Benskey
Viral Vectors for Gene Therapy Book PDF

Author : Fredric P. Manfredsson,Matthew J. Benskey
Publisher : Unknown
Page : 328 pages
File Size : 54,9 Mb
Release : 2019
Category : Electronic books
ISBN : 1493990659

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Viral Vectors for Gene Therapy by Fredric P. Manfredsson,Matthew J. Benskey Pdf

This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, construction, and application of transcription activation-like effectors; multi-modal production of adeno-associated virus; construction of oncolytic herpes simplex virus; AAV-mediated gene delivery to the mouse liver; and intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Viral Vectors for Gene Therapy: Methods and Protocols is a valuable resource for researchers, clinicians, and students looking to utilize viral vectors in gene therapy experiments.