Basic Techniques In Molecular Biology

Author : Stefan Surzycki
Publisher : Springer Science & Business Media
Page : 434 pages
File Size : 45,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642569685

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Basic Techniques in Molecular Biology by Stefan Surzycki Pdf

This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Author : Heather Miller,D. Scott Witherow,Sue Carson
Publisher : Academic Press
Page : 232 pages
File Size : 45,6 Mb
Release : 2011-10-18
Category : Science
ISBN : 9780123855459

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Molecular Biology Techniques by Heather Miller,D. Scott Witherow,Sue Carson Pdf

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Author : Leonard G. Davis,Mark D. Dibner,James F. Battey
Publisher : Unknown
Page : 404 pages
File Size : 43,9 Mb
Release : 1992
Category : Molecular biology
ISBN : IND:30000121511939

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Basic Methods in Molecular Biology by Leonard G. Davis,Mark D. Dibner,James F. Battey Pdf

The new edition of this popular book emphasizes the decisions that need to be made to select one procedure over another.

Author : Walt Ream,Katharine G. Field
Publisher : Academic Press
Page : 245 pages
File Size : 41,7 Mb
Release : 1998-11-17
Category : Science
ISBN : 9780080536828

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Molecular Biology Techniques by Walt Ream,Katharine G. Field Pdf

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Provides well-tested experimental protocols for each technique Lists the reagents and preparation of each experiment separately Contains a complete schedule of experiments and the preparation required Includes study questions at the end of each chapter

Author : J.M. Walker,W. Gaastra
Publisher : Springer Science & Business Media
Page : 304 pages
File Size : 48,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401165631

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Techniques in Molecular Biology by J.M. Walker,W. Gaastra Pdf

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.

Author : Leonard Davis
Publisher : Elsevier
Page : 399 pages
File Size : 46,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780444601490

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Basic Methods in Molecular Biology by Leonard Davis Pdf

Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.

Author : Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Publisher : Academic Press
Page : 220 pages
File Size : 51,8 Mb
Release : 2020-10-28
Category : Science
ISBN : 9780128244500

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Advanced Methods in Molecular Biology and Biotechnology by Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool Pdf

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Author : Surzycki
Publisher : Unknown
Page : 434 pages
File Size : 44,5 Mb
Release : 2007-10-01
Category : Electronic
ISBN : 8181287967

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Basic Techniques In Molecular Biology by Surzycki Pdf

Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Page : 769 pages
File Size : 54,7 Mb
Release : 2010-03-04
Category : Medical
ISBN : 9780521516358

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Principles and Techniques of Biochemistry and Molecular Biology by Keith Wilson,John Walker Pdf

Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Author : Aakanchha Jain,Richa Jain,Sourabh Jain
Publisher : Humana
Page : 282 pages
File Size : 55,8 Mb
Release : 2021-03-14
Category : Medical
ISBN : 1493998633

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Basic Techniques in Biochemistry, Microbiology and Molecular Biology by Aakanchha Jain,Richa Jain,Sourabh Jain Pdf

This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment’s potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.

Author : Friedhelm Hildebrandt,Peter Igarashi
Publisher : Springer Science & Business Media
Page : 394 pages
File Size : 48,5 Mb
Release : 2013-11-11
Category : Science
ISBN : 9783642598111

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Techniques in Molecular Medicine by Friedhelm Hildebrandt,Peter Igarashi Pdf

This manual not only provides reliable, up-to-date protocols for lab use but also the theoretical background of molecular biology, allowing users to better understand the principles underlying these techniques. It covers a wide range of methods, including the purification of nucleic acids, enzymatic modification of DNA, isolation of specific DNA fragments, PCR, cloning techniques, and gene expression. A Springer Lab Manual

Author : Susan J. Karcher
Publisher : Academic Press
Page : 280 pages
File Size : 52,7 Mb
Release : 1995-11-28
Category : Science
ISBN : 0080536832

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Molecular Biology by Susan J. Karcher Pdf

This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Includes a glossary of over 200 terms important for understanding molecular biology Uses an inexpensive source of eukaryotic cells - great for schools on a budget Includes Methods Locator that provides instant access to the latest methods Contain clearly written, easy-to-follow, student-tested instructions: Sterile techniques Phage titration Gel electrophoresis of DNA Restriction enzyme digestion Plasmid isolation Transformation of E. Coli Recombinant DNA cloning Nick translation labeling Nonradioactive primer labelling Nonradioactive DNA detection Southern blotting Colony hybridization Purification of plant DNA RNA purification Northern blotting Purification of poly A+ RNA Polymerase chain reaction (PCR)

Author : P. G. Jones,J. M. Sutton
Publisher : Unknown
Page : 248 pages
File Size : 54,5 Mb
Release : 1997-10-09
Category : Nature
ISBN : UOM:39015039881936

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Plant Molecular Biology by P. G. Jones,J. M. Sutton Pdf

The authors describe the most important currently used protocols in plant molecular biology. They give useful tips on stopping points and troubleshooting as well as providing information on safety. Lists of suppliers are included.

Author : Ricardo V. Lloyd
Publisher : Springer Science & Business Media
Page : 435 pages
File Size : 42,5 Mb
Release : 2001-06-15
Category : Medical
ISBN : 9781592591909

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Morphology Methods by Ricardo V. Lloyd Pdf

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

Author : Bal Ram Singh,Raj Kumar
Publisher : Cambridge University Press
Page : 378 pages
File Size : 46,7 Mb
Release : 2022-06-16
Category : Medical
ISBN : 9781108486408

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Practical Techniques in Molecular Biotechnology by Bal Ram Singh,Raj Kumar Pdf

The book will be useful for undergraduate students as a supplementary/reference text in the field of molecular biotechnology.