Advanced Methods In Molecular Biology And Biotechnology

Author : Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Publisher : Academic Press
Page : 220 pages
File Size : 51,5 Mb
Release : 2020-10-28
Category : Science
ISBN : 9780128244500

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Advanced Methods in Molecular Biology and Biotechnology by Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool Pdf

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Author : Heather Miller,D. Scott Witherow,Sue Carson
Publisher : Academic Press
Page : 232 pages
File Size : 41,5 Mb
Release : 2011-10-18
Category : Science
ISBN : 9780123855459

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Molecular Biology Techniques by Heather Miller,D. Scott Witherow,Sue Carson Pdf

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Author : Bernard R. Glick
Publisher : CRC Press
Page : 382 pages
File Size : 42,9 Mb
Release : 2018-05-04
Category : Science
ISBN : 9781351082945

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Methods in Plant Molecular Biology and Biotechnology by Bernard R. Glick Pdf

Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods - such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.

Author : Sue Carson,Heather Miller,Melissa C. Srougi,D. Scott Witherow
Publisher : Academic Press
Page : 294 pages
File Size : 53,5 Mb
Release : 2019-03-05
Category : Science
ISBN : 9780128157756

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Molecular Biology Techniques by Sue Carson,Heather Miller,Melissa C. Srougi,D. Scott Witherow Pdf

Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions

Author : Bal Ram Singh,Raj Kumar
Publisher : Cambridge University Press
Page : 378 pages
File Size : 45,7 Mb
Release : 2022-06-16
Category : Medical
ISBN : 9781108486408

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Practical Techniques in Molecular Biotechnology by Bal Ram Singh,Raj Kumar Pdf

The book will be useful for undergraduate students as a supplementary/reference text in the field of molecular biotechnology.

Author : Seung-Beom Hong,M. Bazlur Rashid,Lory Z. Santiago-Vázquez
Publisher : John Wiley & Sons
Page : 386 pages
File Size : 49,6 Mb
Release : 2016-08-01
Category : Science
ISBN : 9781119156789

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Methods in Biotechnology by Seung-Beom Hong,M. Bazlur Rashid,Lory Z. Santiago-Vázquez Pdf

As rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods; Methods in Biotechnology is an invaluable resource for those students and professionals. Methods in Biotechnology engages the reader by implementing an active learning approach, provided advanced study questions, as well as pre- and post-lab questions for each lab protocol. These self-directed study sections encourage the reader to not just perform experiments but to engage with the material on a higher level, utilizing critical thinking and troubleshooting skills. This text is broken into three sections based on level – Methods in Biotechnology, Advanced Methods in Biotechnology I, and Advanced Methods in Biotechnology II. Each section contains 14-22 lab exercises, with instructor notes in appendices as well as an answer guide as a part of the book companion site. This text will be an excellent resource for both students and laboratory professionals in the biotechnology field.

Author : R C Dubey
Publisher : S. Chand Publishing
Page : 1161 pages
File Size : 44,9 Mb
Release : 2014
Category : Science
ISBN : 9788121942904

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Advanced Biotechnology by R C Dubey Pdf

The book embodies 22 chapters covering various important disciplines of biotechnology, such as cell biology, molecular biology, molecular genetics, biophysical methods, genomics and proteomics, metagenomics, enzyme technology, immune-technology, transgenic plants and animals, industrial microbiology and environmental biotechnology. The book is illustrative. It is written in a simple language

Author : David Ronald Murray
Publisher : Oxford University Press, USA
Page : 392 pages
File Size : 41,8 Mb
Release : 1991
Category : Medical
ISBN : UOM:39015025290597

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Advanced Methods in Plant Breeding and Biotechnology by David Ronald Murray Pdf

Breeding plants for the twenty-first century; Chromosomal organization and gene mapping; Gene transfer to plants using Agrobacterium; Electroporation for direct gene transfer into plant protoplasts; Microprojectile techniques for direct gene transfer into intact plant cells; Localization of transferred genes in genetically modifically modified plants; Somatic embryogenesis: potential for use in propagation and gene transfer systems; Chloroplast and mitochondrial genomes: manipulation through somatic hybridization; Modification of the chloroplast genome with particular reference to herbicide resistance; Breeding for resistance to insects; Resistance to fungal diseases; Advances in breeding for resistance to bacterial pathogens; Genetic engineering for resistance to viruses; Breeding for resistance to physiological stresses.

Author : Corinne A. Michels
Publisher : John Wiley & Sons
Page : 264 pages
File Size : 53,7 Mb
Release : 2002-06-10
Category : Science
ISBN : 0471899194

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Genetic Techniques for Biological Research by Corinne A. Michels Pdf

Molecular Genetic Analysis is an advanced textbook to teach the theory and practice of molecular genetic analysis to senior undergraduates and graduates studying genetics, molecular biology and cell biology. This book uses a case study approach, with the yeast Saccharomyces as the model genetic organism, to explain the theory and practice of molecular genetic analysis. It provides enough information so readers will be able to apply the approach to their own research project.

Author : Rakesh S. Sengar,Amit Kumar,Reshu Chaudhary,Ashu Singh
Publisher : CRC Press
Page : 436 pages
File Size : 48,9 Mb
Release : 2018-05-11
Category : Medical
ISBN : 9781351021210

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Advances in Molecular Techniques by Rakesh S. Sengar,Amit Kumar,Reshu Chaudhary,Ashu Singh Pdf

Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists.This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering.

Author : S.L. Salzberg,D.B. Searls,S. Kasif
Publisher : Elsevier
Page : 368 pages
File Size : 43,9 Mb
Release : 1998-06-19
Category : Computers
ISBN : 0080860931

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Computational Methods in Molecular Biology by S.L. Salzberg,D.B. Searls,S. Kasif Pdf

Computational biology is a rapidly expanding field, and the number and variety of computational methods used for DNA and protein sequence analysis is growing every day. These algorithms are extremely valuable to biotechnology companies and to researchers and teachers in universities. This book explains the latest computer technology for analyzing DNA, RNA, and protein sequences. Clear and easy to follow, designed specifically for the non-computer scientist, it will help biologists make better choices on which algorithm to use. New techniques and demonstrations are elucidated, as are state-of-the-art problems, and more advanced material on the latest algorithms. The primary audience for this volume are molecular biologists working either in biotechnology companies or academic research environments, individual researchers and the institutions they work for, and students. Any biologist who relies on computers should want this book. A secondary audience will be computer scientists developing techniques with applications in biology. An excellent reference for leading techniques, it will also help introduce computer scientists to the biology problems. This is an outstanding work which will be ideal for the increasing number of scientists moving into computational biology.

Author : Richa Salwan,Vivek Sharma
Publisher : Elsevier
Page : 208 pages
File Size : 55,8 Mb
Release : 2023-06-13
Category : Science
ISBN : 9780323950794

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Laboratory Methods in Microbiology and Molecular Biology by Richa Salwan,Vivek Sharma Pdf

Laboratory Methods in Microbiology and Molecular Biology describes various microbiological, biochemical, and molecular methods employed for the characterization, identification, and analysis of actinomycetes, bacteria and fungi. The book details general guidelines, expectations, and responsibilities for good lab practices and consists of chapters that covers basic microbiological, physiological, biochemical, and molecular aspects as well as in silico analysis using various bioinformatic tools. Other topics in the book include how to make solutions, microscopy and imaging of microorganisms, sero-diagnostics, and basic concepts of phylogeny, physiology, biotechnology, soil, food, and environmental microbiology while working in laboratory. Laboratory Methods in Microbiology and Molecular Biology is an informative update to current practices and future perspectives for the field of microbial biotechnology. It aims to facilitate professors, researchers, and graduate students in monitoring the precision and accuracy of the qualitative and quantitative methods in their research. Involves various procedures in diverse disciplines, from microbiology to genetics, molecular biology and biochemistry Lists the principles and facts underlying practical applications of bacteria and fungi which have prospects in various technologies Includes the questions ‘how’ and ‘why’ as an explanation for novice students and researchers to modify protocols Facilitates students, teachers and researchers to monitor the precision and accuracy of their qualitative and quantitative methods practically

Author : Michael Wink
Publisher : John Wiley & Sons
Page : 711 pages
File Size : 55,7 Mb
Release : 2013-11-14
Category : Science
ISBN : 9783527672035

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An Introduction to Molecular Biotechnology by Michael Wink Pdf

Molecular biotechnology continues to triumph, as this textbook testifies - edited by one of the academic pioneers in the field and written by experienced professionals. This completely revised second edition covers the entire spectrum, from the fundamentals of molecular and cell biology, via an overview of standard methods and technologies, the application of the various "-omics", and the development of novel drug targets, right up to the significance of system biology in biotechnology. The whole is rounded off by an introduction to industrial biotechnology as well as chapters on company foundation, patent law and marketing. The new edition features: - Large format and full color throughout - Proven structure according to basics, methods, main topics and economic perspectives - New sections on system biology, RNA interference, microscopic techniques, high throughput sequencing, laser applications, biocatalysis, current biomedical applications and drug approval - Optimized teaching with learning targets, a glossary containing around 800 entries, over 500 important abbreviations and further reading. The only resource for those who are seriously interested in the topic. Bonus material available online free of charge: www.wiley-vch.de/home/molecbiotech

Author : Alfred Pühler,Kenneth N. Timmis
Publisher : Springer Science & Business Media
Page : 353 pages
File Size : 55,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642693052

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Advanced Molecular Genetics by Alfred Pühler,Kenneth N. Timmis Pdf

The development of powerful new techniques and refmements of tech niques in molecular genetics in recent years, and the surge in interest in biotechnology based on genetic methods, have heralded a new golden age in molecular genetics, and stimulated in diverse disciplines much interest in the technologies themselves and their potential uses in basic and applied biomedical sciences. Although some excellent specialist laboratory manuals (especially the Cold Spring Harbor Laboratory manuals by I. H. Miller; R. W. Davies et al. ; and T. Maniatis et al. ) on certain chapters of molecular genetics exist, no general text that covers a broad spectrum of the sub ject has thus far been published. The purpose of this manual is to pre sent most, though of necessity not all of the important methods of molecular genetics, in a series of simple experiments, many of which can be readily accomplished by the microbiologist, biochemist or biotechnologist that has had only limited exposure to genetics. The remainder of the experiments require either greater familiarity with the subject, or guidance by someone with such experience. The book should, therefore, not only enable individuals to acquire new proce dures for ongoing projects, but also serve as a basis for the teaching of molecular genetic techniques in formal predoctoral and postdoctoral laboratory courses.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 50,6 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf