Bioseparations Of Proteins

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Bioseparations of Proteins

Ajit Sadana
Bioseparations of Proteins Book PDF

Author : Ajit Sadana
Publisher : Elsevier
Page : 346 pages
File Size : 50,6 Mb
Release : 1997-11-27
Category : Science
ISBN : 0080528147

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Bioseparations of Proteins by Ajit Sadana Pdf

This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation. Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations. Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures. Key Features * Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include: * detailed data on biological stability under various bioseparation conditions * extensive case studies from the literature on separation processes, validation, and economics * simplified analysis of protein refolding and inactivation mechanisms * consideration of adsorption theories and the effect of heterogeneity * coverage of both classical and novel bioseparation techniques, including chromatographic procedures

Protein Bioseparation Using Ultrafiltration

Raja Ghosh
Protein Bioseparation Using Ultrafiltration Book PDF

Author : Raja Ghosh
Publisher : World Scientific Publishing Company
Page : 166 pages
File Size : 50,7 Mb
Release : 2003-01-01
Category : Science
ISBN : 1860943179

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Protein Bioseparation Using Ultrafiltration by Raja Ghosh Pdf

Ultrafiltration (UF) is a pressure-driven separation process in which membranes are used for a broad variety of applications, ranging from the processing of biological macromolecules to wastewater treatment. It has significant advantages over competing separation technologies. Food and biotechnological applications of UF account for nearly 40 percent of the current total usage. In the case of high value therapeutic protein and DNA products, the separation and purification costs can be as high as 80 percent of the total cost of production. Therefore, it makes economic sense to develop cost-effective and scaleable purification processes for such products. UF is used for protein concentration, protein desalting and protein fractionation (such as protein-protein separation). Concentration and desalting processes are technologically less demanding and have been widely used in the bioprocess industry for quite some time. Protein fractionation, on the other hand, is a challenging proposition and is definitely a more recent development. This text focuses primarily on protein fractionation.

Process Scale Bioseparations for the Biopharmaceutical Industry

Abhinav A. Shukla,Mark R. Etzel,Shishir Gadam
Process Scale Bioseparations for the Biopharmaceutical Industry Book PDF

Author : Abhinav A. Shukla,Mark R. Etzel,Shishir Gadam
Publisher : CRC Press
Page : 600 pages
File Size : 55,5 Mb
Release : 2006-07-07
Category : Medical
ISBN : 9781420016024

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Process Scale Bioseparations for the Biopharmaceutical Industry by Abhinav A. Shukla,Mark R. Etzel,Shishir Gadam Pdf

The biopharmaceutical industry has become an increasingly important player in the global economy, and the success of these products depends on the development and implementation of cost-effective, robust and scaleable production processes. Bioseparations-also called downstream processing- can be a key source of competitive advantageto biopharmaceut

Handbook of Bioseparations

Satinder Ahuja
Handbook of Bioseparations Book PDF

Author : Satinder Ahuja
Publisher : Elsevier
Page : 740 pages
File Size : 55,9 Mb
Release : 2000-06-23
Category : Technology & Engineering
ISBN : 9780080507798

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Handbook of Bioseparations by Satinder Ahuja Pdf

It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories. Key Features * Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies * Encompasses both analytical and process-scale methods * Elucidates the importance of engineering process control * Details selection of plant and process equipment * Addresses economic considerations * Discusses future developments

Molecular Interactions in Bioseparations

That T. Ngo
Molecular Interactions in Bioseparations Book PDF

Author : That T. Ngo
Publisher : Springer Science & Business Media
Page : 560 pages
File Size : 43,9 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781489918727

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Molecular Interactions in Bioseparations by That T. Ngo Pdf

Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.

Bioseparation Processes in Food

Singh
Bioseparation Processes in Food Book PDF

Author : Singh
Publisher : CRC Press
Page : 484 pages
File Size : 47,5 Mb
Release : 1995-05-16
Category : Technology & Engineering
ISBN : 082479608X

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Bioseparation Processes in Food by Singh Pdf

Offers in-depth coverage of the latest advances in new and traditional separation technologies as they are used in a variety of ways to produce value-added products. Examines both fundamental and applied aspects of separation techniques.

Protein Chromatography

Giorgio Carta,Alois Jungbauer
Protein Chromatography Book PDF

Author : Giorgio Carta,Alois Jungbauer
Publisher : John Wiley & Sons
Page : 440 pages
File Size : 54,9 Mb
Release : 2020-06-02
Category : Science
ISBN : 9783527346660

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Protein Chromatography by Giorgio Carta,Alois Jungbauer Pdf

An all-in-one practical guide on how to efficiently use chromatographic separation methods Based on a training course that teaches the theoretical as well as practical aspects of protein bioseparation to bioprocess professionals, this fully updated and revised new edition offers comprehensive coverage of continuous chromatography and provides readers with many relevant examples from the biopharmaceutical industry. Divided into two large parts, Protein Chromatography: Process Development and Scale-Up, Second Edition presents all the necessary knowledge for effective process development in chromatographic bioseparation, both on small and large scale. The first part introduces chromatographic theory, including process design principles, to enable the reader to rationalize the set-up of a bioseparation process. The second part illustrates by way of case studies and sample protocols how the theory learned in the first part may be applied to real-life problems. Chapters look at: Downstream Processing of Biotechnology Products; Chromatography Media; Laboratory and Process Columns and Equipment; Adsorption Equilibrium; Rate Processes; and Dynamics of Chromatography Columns. The book closes with chapters on: Effects of Dispersion and Rate Processes on Column Performance; Gradient Elution Chromatography; and Chromatographic Column Design and Optimization. -Presents the most pertinent examples from the biopharmaceutical industry, including monoclonal antibodies -Provides an overview of the field along with design tools and examples illustrating the advantages of continuous processing in biopharmaceutical productions -Focuses on process development and large-scale bioseparation tasks, making it an ideal guide for the professional bioengineer in the biotech and pharma industries -Offers field-tested information based on decades of training courses for biotech and chemical engineers in Europe and the U.S. Protein Chromatography: Process Development and Scale-Up, Second Edition will appeal to biotechnologists, analytical chemists, chromatographers, chemical engineers, pharmaceutical industry, biotechnological industry, and biochemists.

Isolation and Purification of Proteins

Rajni Hatti-Kaul,Bo Mattiasson
Isolation and Purification of Proteins Book PDF

Author : Rajni Hatti-Kaul,Bo Mattiasson
Publisher : CRC Press
Page : 751 pages
File Size : 46,5 Mb
Release : 2003-02-05
Category : Medical
ISBN : 9781135545246

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Isolation and Purification of Proteins by Rajni Hatti-Kaul,Bo Mattiasson Pdf

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host

Engineering Processes for Bioseparations

LAURENCE R. WEATHERLEY
Engineering Processes for Bioseparations Book PDF

Author : LAURENCE R. WEATHERLEY
Publisher : Elsevier
Page : 250 pages
File Size : 52,7 Mb
Release : 2013-10-22
Category : Technology & Engineering
ISBN : 9781483292106

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Engineering Processes for Bioseparations by LAURENCE R. WEATHERLEY Pdf

The use of biotechnology in chemical synthesis offers up numerous advantages to the engineer in the process industries, but it also presents a number of fundamental challenges and difficulties which impinge directly on separation process requirements. The use of biochemical separations has grown significantly during the past decade, and is especially used in process industries such as healthcare and food processing. However it is becoming increasingly more important in areas such as recycling and waste-water treatment and as industry shifts towards cleaner processes biochemical separations will continue to grow. The two main objectives of this book are to focus on the application of existing separation process techniques to the recovery and purification of biologically derived products and to examine the state of knowledge of new techniques which have future potential. Within these objectives the complexities and breadth of problems associated with biological separations are discussed, specific engineering techniques are featured and their adaptation to biochemical separations are highlighted.

Bioseparations Science and Engineering

Roger G. Harrison,Paul Todd,Scott R. Rudge,Demetri P. Petrides
Bioseparations Science and Engineering Book PDF

Author : Roger G. Harrison,Paul Todd,Scott R. Rudge,Demetri P. Petrides
Publisher : Topics in Chemical Engineering
Page : 577 pages
File Size : 45,6 Mb
Release : 2015
Category : Technology & Engineering
ISBN : 9780195391817

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Bioseparations Science and Engineering by Roger G. Harrison,Paul Todd,Scott R. Rudge,Demetri P. Petrides Pdf

Preceded by: Bioseparations science and engineering / Roger G. Harrison ... [et al.]. c2003.

Bioseparations

Paul A. Belter,E. L. Cussler,Wei-Shou Hu
Bioseparations Book PDF

Author : Paul A. Belter,E. L. Cussler,Wei-Shou Hu
Publisher : Wiley-Interscience
Page : 392 pages
File Size : 45,9 Mb
Release : 1988-02-22
Category : Science
ISBN : UOM:39015012755750

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Bioseparations by Paul A. Belter,E. L. Cussler,Wei-Shou Hu Pdf

Offers a concise introduction to the separation and purification of biochemicals. Bridges two scientific cultures, providing an introduction to bioseparations for scientists with no background in engineering and for engineers with little grounding in biology. The authors supplement the ideas by simple worked examples, making the techniques of bioseparations easy to learn. Discusses removal of insolubles, product isolation, purification and polishing.

Principles of Bioseparations Engineering

Raja Ghosh
Principles of Bioseparations Engineering Book PDF

Author : Raja Ghosh
Publisher : World Scientific Publishing Company
Page : 284 pages
File Size : 45,5 Mb
Release : 2006-10-23
Category : Technology & Engineering
ISBN : 9789813106765

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Principles of Bioseparations Engineering by Raja Ghosh Pdf

Bioseparations engineering deals with the scientific and engineering principles involved in large-scale separation and purification of biological products. It is a key component of most chemical engineering/biotechnology/bioprocess engineering programmes. This book discusses the underlying principles of bioseparations engineering written from the perspective of an undergraduate course. It covers membrane based bioseparations in much more detail than some of the other books on bioseparations engineering. Based largely on the lecture notes the author developed to teach the course, this book is especially suitable for use as an undergraduate level textbook, as most other textbooks are targeted at graduate students.

Protein Chromatography

Giorgio Carta,Alois Jungbauer
Protein Chromatography Book PDF

Author : Giorgio Carta,Alois Jungbauer
Publisher : John Wiley & Sons
Page : 365 pages
File Size : 50,9 Mb
Release : 2010-06-08
Category : Science
ISBN : 9783527318193

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Protein Chromatography by Giorgio Carta,Alois Jungbauer Pdf

With its focus on process development and large-scale bioseparation tasks, this is tailor-made reading for the professional bioengineer in both the biotech and pharmaceutical industries. Following a tried-and-tested concept, this guide has been developed over several years in training courses for biotech and chemical engineers in Europe and the U.S. The first part deals with the theory, introducing chromatography and its dynamics, as well as discussing mass transfer and dispersion effects. The second part then goes on to cover equipment and protocols, determining the retention factor and HETP from isocratic and elution experiments, as well as the mass transfer and intraparticle diffusivity from batch and shallow-bed adsorption experiments.

Process Scale Purification of Antibodies

Uwe Gottschalk
Process Scale Purification of Antibodies Book PDF

Author : Uwe Gottschalk
Publisher : John Wiley & Sons
Page : 752 pages
File Size : 49,5 Mb
Release : 2017-03-07
Category : Medical
ISBN : 9781119126935

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Process Scale Purification of Antibodies by Uwe Gottschalk Pdf

Promoting a continued and much-needed renaissance in biopharmaceutical manufacturing, this book covers the different strategies and assembles top-tier technology experts to address the challenges of antibody purification. • Updates existing topics and adds new ones that include purification of antibodies produced in novel production systems, novel separation technologies, novel antibody formats and alternative scaffolds, and strategies for ton-scale manufacturing • Presents new and updated discussions of different purification technologies, focusing on how they can address the capacity crunch in antibody purification • Emphasizes antibodies and innovative chromatography methods for processing

Production of Plasma Proteins for Therapeutic Use

Joseph Bertolini,Neil Goss,John Curling
Production of Plasma Proteins for Therapeutic Use Book PDF

Author : Joseph Bertolini,Neil Goss,John Curling
Publisher : John Wiley & Sons
Page : 518 pages
File Size : 50,5 Mb
Release : 2012-12-26
Category : Medical
ISBN : 9780470924310

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Production of Plasma Proteins for Therapeutic Use by Joseph Bertolini,Neil Goss,John Curling Pdf

Sets forth the state of the science and technology in plasma protein production With contributions from an international team of eighty leading experts and pioneers in the field, Production of Plasma Proteins for Therapeutic Use presents a comprehensive overview of the current state of knowledge about the function, use, and production of blood plasma proteins. In addition to details of the operational requirements for the production of plasma derivatives, the book describes the biology, development, research, manufacture, and clinical indications of essentially all plasma proteins with established clinical use or therapeutic potential. Production of Plasma Proteins for Therapeutic Use covers the key aspects of the plasma fractionation industry in five sections: Section 1: Introduction to Plasma Fractionation initially describes the history of transfusion and then covers the emergence of plasma collection and fractionation from its earliest days to the present time, with the commercial and not-for-profit sectors developing into a multi-billion dollar industry. Section 2: Plasma Proteins for Therapeutic Use contains 24 chapters dedicated to specific plasma proteins, including coagulation factors, albumin, immunoglobulin, and a comprehensive range of other plasma-derived proteins with therapeutic indications. Each chapter discusses the physiology, biochemistry, mechanism of action, and manufacture of each plasma protein including viral safety issues and clinical uses. Section 3: Pathogen Safety of Plasma Products examines issues and procedures for enhancing viral safety and reducing the risk of transmissible spongiform encephalopathy transmission. Section 4: The Pharmaceutical Environment Applied to Plasma Fractionation details the requirements and activities associated with plasma collection, quality assurance, compliance with regulatory requirements, provision of medical affairs support, and the manufacture of plasma products. Section 5: The Market for Plasma Products and the Economics of Fractionation reviews the commercial environment and economics of the plasma fractionation industry including future trends, highlighting regions such as Asia, which have the potential to exert a major influence on the plasma fractionation industry in the twenty-first century.