Calcium In Living Cells

Author : Anonim
Publisher : Academic Press
Page : 328 pages
File Size : 45,5 Mb
Release : 2010-11-26
Category : Science
ISBN : 0080951228

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Calcium in Living Cells by Anonim Pdf

Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases. Gives researchers much needed information on how to study calcium in live cells, which is becoming increasingly important in heart, musculoskeletal, and immune system research Provides an overview of the latest methods--fluorescence resonance energy transfer (FRET), for example-- that are new to the field Allows understanding of how calcium plays a role in intracellular function at the cellular level, which has proved important in Alzheimer's research, heart disease, and areas of musculoskeletal research Updated chapters reflect advancements in the classic techniques used'preparing calcium buffers, vibrating the Ca2+ Electrode and confocal imaging

Author : Anonim
Publisher : Academic Press
Page : 368 pages
File Size : 44,5 Mb
Release : 1994-04-25
Category : Science
ISBN : 0080859380

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A Practical Guide to the Study of Calcium in Living Cells by Anonim Pdf

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes Fluorescent dyes Luminescent proteins Includes techniques for perturbing intracellular Ca2+ Covers detailed methodology plus problems and pitfalls of each technique Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program Color plates illustrate techniques such as Confocal ratio-imaging Use of aequorin

Author : R. Pochet,Rosario Donato,J. Haiech,Claus W. Heizmann,Volker Gerke
Publisher : Springer Science & Business Media
Page : 746 pages
File Size : 48,6 Mb
Release : 2011-06-28
Category : Medical
ISBN : 9789401006880

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Calcium: The molecular basis of calcium action in biology and medicine by R. Pochet,Rosario Donato,J. Haiech,Claus W. Heizmann,Volker Gerke Pdf

The enormous and varied role of calcium in living systems is now widely appreciated by both cell biologists and clinicians. The identification and characterisation of new calcium binding proteins and regulatory pathways is matched by the recognition of the involvement of calcium binding proteins in a growing number of disease states. This book is intended to introduce clinicians to fundamental biological research, whilst at the same time attracting researchers to the clinical world. The publication of the book coincides with the elucidation of the complete Human Genomic Sequence. As a result of this, scientists now have access to an unprecedented array of data, from which new calcium binding proteins and hence new regulatory pathways will undoubtedly be discovered. It is a further aim of this book to provide a `key' to open the door to the new postgenomic era. The book is in three parts. The first section introduces the reader to the role of calcium in cell biology, providing an appreciation of how this small, simple, non-metabolisable agent can move rapidly and silently through the different cellular compartments, thereby influencing and controlling the fate of the cell. This section also illustrates and dissects the often-complex interplay between calcium and numerous agents in muscle and endocrine cells, neurons, hepatocytes, and platelets. In the second section the reader will discover the role of calcium and its partners in common diseases such as migraine and drug dependence. New classes of diseases such as annexinopathies, channelopathies, calcium-sensing disorders, and citrullinemia are discussed, and the authors give many new insights into the molecular mechanisms of the diseases, thereby explaining how and why they occur. Such information is clearly of primary importance for the pharmaceutical industry. New ideas and concepts of neurodegenerative diseases are introduced, which should stimulate new approaches. Clinicians will also have access, in a comprehensive and authoritative yet highly readable chapter, to data from recent large-scale clinical studies on the numerous and widely prescribed calcium antagonists. The final section gives information on new methods and devices for calcium imaging, and illustrates how calcium movement and change can be monitored and ingeniously utilised as a fast, cheap, and accurate drug screening instrument.

Author : Lars Kaestner
Publisher : Springer Science & Business Media
Page : 247 pages
File Size : 49,6 Mb
Release : 2012-12-02
Category : Medical
ISBN : 9783642346170

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Calcium signalling by Lars Kaestner Pdf

The book combines general concepts and methods to investigate calcium signalling in cells ranging from molecular biology approaches to manipulation of calcium in living cells. The focus within these methods in on the broad range of fluorescence imaging technology, in particular on optical sectioning techniques and fast image acquisition. In addition to these general guidelines there are application examples in a context beyond calcium signalling in two major fields: investigations of isolated cardiac myocytes and red blood cell related research. While the cellular cardiology section provides snapshots of certain calcium signalling aspects, the red blood cell part presents an overview from the functional identification of calcium-channels to a concept of physiological and pathophysiological relevance.

Author : Ronald P. Rubin,George B. Weiss,James W. Jr. Putney
Publisher : Springer Science & Business Media
Page : 697 pages
File Size : 40,8 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781461323778

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Calcium in Biological Systems by Ronald P. Rubin,George B. Weiss,James W. Jr. Putney Pdf

This volume is based in part upon the proceedings of the Calcium Theme held during the 67th Annual Meeting of the Federation of American Societies for Experimental Biology, which took place in Chicago, AprillO-lS, 1983. The American Society for Pharmacology and Experimental Therapeutics had the primary responsibility for organizing the scientific program with the assistance of other member societies, including the American Physiology Society, American Association of Pathologists, and American Institute of Nutrition. The purpose of the Calcium Theme was to review progress in the diverse areas of investigation bearing on the ubiquitous role of calcium in biological systems. In addition to contributions from those participating in the Theme, this volume also includes a number of invited papers that were added to fill certain voids in topics covered. The authors were selected because they are investigators active in the mainstream of their particular research area, possessing the acumen to analyze cogently not only their own recent findings but also to relate these findings to their respective area. New information as well as reviews of current concepts generally highlight the individual contributions. Undoubtedly, some readers may argue with the emphasis made and/or the conclusions reached on individual topics. In such cases, other volumes will hopefully provide a forum for alternative points of view. Due to the broad scope of subjects covered and the large number of contributions, the papers have been arranged in three sections.

Author : Anonim
Publisher : Academic Press
Page : 328 pages
File Size : 52,9 Mb
Release : 2010-11-25
Category : Science
ISBN : 0123748410

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Calcium in Living Cells by Anonim Pdf

Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases. Gives researchers much needed information on how to study calcium in live cells, which is becoming increasingly important in heart, musculoskeletal, and immune system research Provides an overview of the latest methods--fluorescence resonance energy transfer (FRET), for example-- that are new to the field Allows understanding of how calcium plays a role in intracellular function at the cellular level, which has proved important in Alzheimer's research, heart disease, and areas of musculoskeletal research Updated chapters reflect advancements in the classic techniques used'preparing calcium buffers, vibrating the Ca2+ Electrode and confocal imaging

Author : Leopold J. Anghileri,Anne Marie Tuffet-Anghileri
Publisher : Unknown
Page : 248 pages
File Size : 43,9 Mb
Release : 1982
Category : Calcium
ISBN : UOM:39015058026405

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The Role of Calcium in Biological Systems by Leopold J. Anghileri,Anne Marie Tuffet-Anghileri Pdf

Author : Juliusz Ashot Kozak,James W. Putney, Jr.
Publisher : CRC Press
Page : 328 pages
File Size : 47,9 Mb
Release : 2021-03-30
Category : Electronic
ISBN : 0367657902

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Calcium Entry Channels in Non-Excitable Cells by Juliusz Ashot Kozak,James W. Putney, Jr. Pdf

Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 49,7 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Alexei Verkhratsky,Ole H. Petersen
Publisher : Humana Press
Page : 256 pages
File Size : 43,5 Mb
Release : 2012-02-25
Category : Medical
ISBN : 1617796778

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Calcium Measurement Methods by Alexei Verkhratsky,Ole H. Petersen Pdf

Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signaling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In "Calcium Measurement Methods", a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful NeuromethodsTM series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field. Cutting-edge and concise, "Calcium Measurement Methods" serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.

Author : Victor R. Preedy
Publisher : Royal Society of Chemistry
Page : 666 pages
File Size : 45,9 Mb
Release : 2015-07-31
Category : Medical
ISBN : 9781849738873

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Calcium by Victor R. Preedy Pdf

Calcium's importance in health and disease is clear when listing its multiple roles in the body, which include building strong bones and teeth, vascular calcification, muscle function, hormonal regulation and maintaining a normal heartbeat. This book will examine these roles and will also cover areas such as chemical analysis, sources of calcium based on geography, influence of Vitamin D, hypercalcemia and the effects of dietary calcium. This edited volume will pool knowledge across scientific disciplines in a way that increases its applicability to a wide range of audiences and fills the gap identified in providing comprehensive synopses of food substances. Chemists, analytical scientists, forensic scientists, food scientists, as well as course lecturers and university librarians, will all benefit from this title.

Author : Mohamed Trebak,Scott Earley
Publisher : CRC Press
Page : 433 pages
File Size : 41,6 Mb
Release : 2018-08-06
Category : Medical
ISBN : 9781498774239

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Signal Transduction and Smooth Muscle by Mohamed Trebak,Scott Earley Pdf

All hollow organs, such as blood vessels, the gastrointestinal tract, airways, male and female reproductive systems, and the urinary bladder are primarily composed of smooth muscle. Such organs regulate flow, propulsion and mixing of luminal contents and storage by the contraction and relaxation of smooth muscle cells. Smooth muscle cells respond to numerous inputs, including pressure, shear stress, intrinsic and extrinsic innervation, hormones and other circulating molecules, as well as autocrine and paracrine factors. This book is a review of smooth muscle cell regulation in the cardiovascular, reproductive, GI, and other organ systems with emphasis on calcium and receptor signaling. Key selling features: Focuses on smooth muscles of different types Describes ion channel signaling mechanisms Reviews calcium and receptor signaling Includes novel, cutting-edge methodologies Summarizes studies of mice with genetically encoding sensors in smooth muscle Chapter 9 of this book is freely available as a downloadable Open Access PDF at http://www.taylorfrancis.com under a Creative Commons Attribution (CC-BY) 4.0 license.

Author : Peter Frederick Baker,Harald Reuter
Publisher : Pergamon
Page : 102 pages
File Size : 54,7 Mb
Release : 1975-01-01
Category : Action potentials (Electrophysiology).
ISBN : 0080182984

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Calcium Movement in Excitable Cells by Peter Frederick Baker,Harald Reuter Pdf

Calcium Movement in Excitable Cells, which is a second in a series, is a collection of articles taken from articles published in Progress in Biophysics and Molecular Biology, just like the first. The monograph is divided into two chapters. Chapter 1, Transport and Metabolism of Calcium Ions in Nerve, tackles the mechanisms responsible for maintaining the electrochemical gradient for calcium and effecting changes for the permeability of the cell membrane to calcium ions. Chapter 2, Divalent Cations as Charge Carriers in Excitable Membranes, tries to find out if divalent cations such as calcium,...

Author : Jonathan Soboloff,Dietmar J. Kappes
Publisher : CRC Press
Page : 258 pages
File Size : 48,7 Mb
Release : 2017-03-27
Category : Medical
ISBN : 9781498705097

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Signaling Mechanisms Regulating T Cell Diversity and Function by Jonathan Soboloff,Dietmar J. Kappes Pdf

T cells play a vital role mediating adaptive immunity, a specific acquired resistance to an infectious agent produced by the introduction of an antigen. There are a variety of T cell types with different functions. They are called T cells, because they are derived from the thymus gland. This volume discusses how T cells are regulated through the operation of signaling mechanisms. Topics covered include positive and negative selection, early events in T cell receptor engagement, and various T cell subsets.

Author : Martin Falcke,Dieter Malchow
Publisher : Springer
Page : 300 pages
File Size : 55,6 Mb
Release : 2014-03-12
Category : Science
ISBN : 3662143984

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Understanding Calcium Dynamics by Martin Falcke,Dieter Malchow Pdf

Written as a set of tutorial reviews on both experimental facts and theoretical modelling, this volume is intended as an introduction and modern reference in the field for graduate students and researchers in biophysics, biochemistry and applied mathematics.