Capillary Electrophoresis Principles And Practice

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Capillary Electrophoresis: Principles and Practice

Reinhard Kuhn,Sabrina Hoffstetter-Kuhn
Capillary Electrophoresis Principles and Practice Book PDF

Author : Reinhard Kuhn,Sabrina Hoffstetter-Kuhn
Publisher : Springer Science & Business Media
Page : 381 pages
File Size : 44,7 Mb
Release : 2013-03-07
Category : Science
ISBN : 9783642780585

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Capillary Electrophoresis: Principles and Practice by Reinhard Kuhn,Sabrina Hoffstetter-Kuhn Pdf

Capillary electrophoresis (CE) is a brand-new analytical me- thod with the capability of solving many analytical separa- tion problems very fast and economically. This method gives new information about the investigated substances which can- not easily be obtained by other means. CE has become an established method only recently, but will be implemented in almost every analytical laboratory in industry, service units and academia in the near future. The most important fields of CE application are pharmaceutical and biochemical research and quality control. The authors have exhaustive practical experience in the application of CE methods in the pharmaceutical industry and provide the reader with a comprehensive treatment of this method. The main focus is on how to solve problems when applying CE in the laboratory. Physico-chemical theory is only dealt with in depth when necessary to understand the underlying separation mechanisms in order to solve your problems at the analytical bench. An addendum includes tables on the preparation of buffers and recommended further reading.

Capillary Electrophoresis

S.F.Y. Li
Capillary Electrophoresis Book PDF

Author : S.F.Y. Li
Publisher : Elsevier
Page : 581 pages
File Size : 44,6 Mb
Release : 1992-07-31
Category : Science
ISBN : 0080858600

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Capillary Electrophoresis by S.F.Y. Li Pdf

Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE have been reported. Rapid, reproducible separations of extremely small amounts of chemicals and biochemicals, including peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, vitamins, inorganic ions, pharmaceuticals and environmental pollutants have been demonstrated. A wide range of applications have been developed in greatly diverse fields, such as chemical, biotechnological, environmental and pharmaceutical analysis. This book covers all aspects of CE, from the principles and technical aspects to the most important applications. It is intended to meet the growing need for a thorough and balanced treatment of CE. The book will serve as a comprehensive reference work. Both the experienced analyst and the newcomer will find the text useful.

High Performance Liquid Chromatography & Capillary Electrophoresis

Andrea Weston,Phyllis R. Brown
High Performance Liquid Chromatography Capillary Electrophoresis Book PDF

Author : Andrea Weston,Phyllis R. Brown
Publisher : Elsevier
Page : 280 pages
File Size : 44,7 Mb
Release : 1997-07-21
Category : Science
ISBN : 0080534163

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High Performance Liquid Chromatography & Capillary Electrophoresis by Andrea Weston,Phyllis R. Brown Pdf

HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity. Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques Applicable to a wide variety of separation problems Covers basic concepts governing any separation as well as instrumentation and how to use it Helps the user to obtain optimal resolution in minimal time Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation Includes information on upcoming trends such as miniaturization Major concepts in each chapter are organized to allow access to information easily and quickly Contains practical bibliography for accessing the literature

Capillary Electrophoresis Guidebook

Kevin D. Altria
Capillary Electrophoresis Guidebook Book PDF

Author : Kevin D. Altria
Publisher : Springer Science & Business Media
Page : 347 pages
File Size : 41,8 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595396

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Capillary Electrophoresis Guidebook by Kevin D. Altria Pdf

This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro vided for individual analyses. The intention is to provide general guide lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.

Capillary Electrophoresis

Patrick Camilleri
Capillary Electrophoresis Book PDF

Author : Patrick Camilleri
Publisher : CRC Press
Page : 586 pages
File Size : 43,9 Mb
Release : 1997-11-25
Category : Medical
ISBN : 084939127X

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Capillary Electrophoresis by Patrick Camilleri Pdf

The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material. Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.

Capillary Electrophoresis

Paul D. Grossman,Joel C. Colburn
Capillary Electrophoresis Book PDF

Author : Paul D. Grossman,Joel C. Colburn
Publisher : Academic Press
Page : 369 pages
File Size : 41,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323138192

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Capillary Electrophoresis by Paul D. Grossman,Joel C. Colburn Pdf

This book is designed to be a practical guide, used by wide audience, including those new to CE, those more experienced, routine users, those interested in technology development, and those involved with applications research. References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations. This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on:Electroosmosis, separation efficiency, and Joule heatingDetection methodsIn-depth discussion of the separation principles and capabilities of the major modes of CESieving gel electrophoresisIsoelectric focusingFree solution CEMicellar electrokinetic capillary chromatographyEntangled polymer matrix-based separationDetailed treatment of the application of CE to a wide range of molecules, supplemented with extensive "hands-on" illustrations

Capillary Electrophoresis in Chiral Analysis

Bezhan Chankvetadze
Capillary Electrophoresis in Chiral Analysis Book PDF

Author : Bezhan Chankvetadze
Publisher : John Wiley & Sons
Page : 586 pages
File Size : 46,8 Mb
Release : 1997-12-29
Category : Science
ISBN : 0471974153

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Capillary Electrophoresis in Chiral Analysis by Bezhan Chankvetadze Pdf

Das weltweite Interesse an der Trennung von Enantiomeren, Spiegelbildern ein und derselben chemischen Verbindung, nimmt zu, da diese Formen beispielsweise verschiedene physiologische Wirkungen hervorrufen können. Eine hochaktuelle Technik der Stofftrennung in diesem Zusammenhang ist die Kapillarelektrophorese, die hier von den theoretischen Grundlagen bis hin zu analytischen Details und Anbieterlisten ausführlich beschrieben wird.

Capillary Electrophoresis

Dale R. Baker
Capillary Electrophoresis Book PDF

Author : Dale R. Baker
Publisher : Wiley-Interscience
Page : 280 pages
File Size : 44,5 Mb
Release : 1995-03-02
Category : Science
ISBN : UOM:39015034442098

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Capillary Electrophoresis by Dale R. Baker Pdf

The best source of practical, easily accessible information on this exciting new technique CAPILLARY ELECTROPHORESIS Analytical chemists and biochemists have been turning to the technique of capillary electrophoresis with increasing frequency: it is fast, sensitive, easy to automate, requires only small sample volumes and reagent amounts, and has been successfully applied to an ever-expanding list of sample types. In Capillary Electrophoresis, analytical practitioners will find a complete, practical guide to the principles, forms, and instrumentation of this technique. The book presents clear and straightforward explanations of the method, its operating principles, and its different modes, including capillary zone electrophoresis, micellar electrokinetic capillary chromatography, capillary gel electrophoresis, capillary isoelectric focusing, and capillary isotachophoresis. Especially helpful is the material on developing a method: it offers practical guidance on CE modes, capillaries, run buffers, voltage requirements, sample pretreatments, injection modes and amounts, temperature settings, detector selections, buffer replenishment, and data reporting. Capillary Electrophoresis will serve both as an excellent introduction to those who are new to the technique and as a comprehensive reference book to experienced practitioners. A complete and detailed index will assist the reader in quickly finding any topic of interest.

Capillary Electrophoresis and Microchip Capillary Electrophoresis

Carlos D. García,Karin Y. Chumbimuni-Torres,Emanuel Carrilho
Capillary Electrophoresis and Microchip Capillary Electrophoresis Book PDF

Author : Carlos D. García,Karin Y. Chumbimuni-Torres,Emanuel Carrilho
Publisher : John Wiley & Sons
Page : 638 pages
File Size : 51,6 Mb
Release : 2013-02-01
Category : Science
ISBN : 9781118529997

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Capillary Electrophoresis and Microchip Capillary Electrophoresis by Carlos D. García,Karin Y. Chumbimuni-Torres,Emanuel Carrilho Pdf

Explores the benefits and limitations of the latest capillary electrophoresis techniques Capillary electrophoresis and microchip capillary electrophoresis are powerful analytical tools that are particularly suited for separating and analyzing biomolecules. In comparison with traditional analytical techniques, capillary electrophoresis and microchip capillary electrophoresis offer the benefits of speed, small sample and solvent consumption, low cost, and the possibility of miniaturization. With contributions from a team of leading analytical scientists, Capillary Electrophoresis and Microchip Capillary Electrophoresis explains how researchers can take full advantage of all the latest techniques, emphasizing applications in which capillary electrophoresis has proven superiority over other analytical approaches. The authors not only explore the benefits of each technique, but also the limitations, enabling readers to choose the most appropriate technique to analyze a particular sample. The book's twenty-one chapters explore fundamental aspects of electrophoretically driven separations, instrumentation, sampling techniques, separation modes, detection systems, optimization strategies for method development, and applications. Specific topics include: Critical evaluation of the use of surfactants in capillary electrophoresis Sampling and quantitative analysis in capillary electrophoresis Capillary electrophoresis with electrochemical detection Overcoming challenges in using microchip electrophoresis for extended monitoring applications Capillary electrophoresis of intact unfractionated heparin and related impurities Microchip capillary electrophoresis for in situ planetary exploration Each chapter begins with an introduction and ends with conclusions as well as references to the primary literature. Novices to the field will find this book an easy-to-follow introduction to core capillary electrophoresis techniques and methods. More experienced investigators can turn to the book for troubleshooting tips and expert advice to guide them through the most advanced applications.

Capillary Electrophoresis Guidebook

Kevin D. Altria
Capillary Electrophoresis Guidebook Book PDF

Author : Kevin D. Altria
Publisher : Humana Press
Page : 349 pages
File Size : 51,9 Mb
Release : 2010-10-28
Category : Science
ISBN : 1617370118

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Capillary Electrophoresis Guidebook by Kevin D. Altria Pdf

This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro vided for individual analyses. The intention is to provide general guide lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.

Clinical and Forensic Applications of Capillary Electrophoresis

John R. Petersen,Amin A. Mohammad
Clinical and Forensic Applications of Capillary Electrophoresis Book PDF

Author : John R. Petersen,Amin A. Mohammad
Publisher : Springer Science & Business Media
Page : 449 pages
File Size : 48,5 Mb
Release : 2001-04-20
Category : Medical
ISBN : 9781592591206

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Clinical and Forensic Applications of Capillary Electrophoresis by John R. Petersen,Amin A. Mohammad Pdf

Because capillary electrophoresis (CE) is driving the automation of manual and semi-manual techniques, conserves precious samples, and minimizes the use of hazardous organic chemicals, it constitutes a powerful new methodology for the clinical laboratory. In Clinical and Forensic Applications of Capillary Electrophoresis, John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and nonaqueous electrophoresis. The authors show how a variety of detection methods (UV-visible, fluorescence, and MS) can be used in conjunction with CE to detect a broad array of analytes, ranging from serum proteins to PCR products to drugs of forensic importance. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.

Clinical Applications of Capillary Electrophoresis

Stephen M. Palfrey
Clinical Applications of Capillary Electrophoresis Book PDF

Author : Stephen M. Palfrey
Publisher : Springer Science & Business Media
Page : 215 pages
File Size : 44,7 Mb
Release : 2008-02-01
Category : Medical
ISBN : 9781592596898

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Clinical Applications of Capillary Electrophoresis by Stephen M. Palfrey Pdf

In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.

Capillary Electrophoresis of Proteins

Tim Wehr,Roberto Rodriguez-Diaz,Mingde Zhu
Capillary Electrophoresis of Proteins Book PDF

Author : Tim Wehr,Roberto Rodriguez-Diaz,Mingde Zhu
Publisher : CRC Press
Page : 301 pages
File Size : 42,9 Mb
Release : 1998-10-20
Category : Science
ISBN : 9781482289855

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Capillary Electrophoresis of Proteins by Tim Wehr,Roberto Rodriguez-Diaz,Mingde Zhu Pdf

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."

Handbook of Capillary Electrophoresis, Second Edition

James P. Landers
Handbook of Capillary Electrophoresis Second Edition Book PDF

Author : James P. Landers
Publisher : CRC Press
Page : 918 pages
File Size : 41,9 Mb
Release : 1996-12-23
Category : Medical
ISBN : 084932498X

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Handbook of Capillary Electrophoresis, Second Edition by James P. Landers Pdf

Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.

Capillary Gel Electrophoresis

Andras Guttman,László Hajba
Capillary Gel Electrophoresis Book PDF

Author : Andras Guttman,László Hajba
Publisher : Newnes
Page : 391 pages
File Size : 54,8 Mb
Release : 2021-12-04
Category : Science
ISBN : 9780080931357

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Capillary Gel Electrophoresis by Andras Guttman,László Hajba Pdf

Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses