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Clinical Applications of Capillary Electrophoresis

Stephen M. Palfrey

Author : Stephen M. Palfrey
Publisher : Springer Science & Business Media
Page : 215 pages
File Size : 55,7 Mb
Release : 2008-02-01
Category : Medical
ISBN : 9781592596898

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Clinical Applications of Capillary Electrophoresis by Stephen M. Palfrey Pdf

In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.

Clinical Applications of Capillary Electrophoresis

Terry M. Phillips,Heather Kalish

Author : Terry M. Phillips,Heather Kalish
Publisher : Humana Press
Page : 328 pages
File Size : 52,9 Mb
Release : 2016-05-01
Category : Science
ISBN : 1493962388

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Clinical Applications of Capillary Electrophoresis by Terry M. Phillips,Heather Kalish Pdf

This Methods in Molecular Biology book is an in-depth manual of Capillary Electrophoresis applications in important areas of clinical science, including clinical chemistry, hematology, disease-associated biomarker discovery, immunology and genetic analysis."

Clinical and Forensic Applications of Capillary Electrophoresis

John R. Petersen,Amin A. Mohammad

Author : John R. Petersen,Amin A. Mohammad
Publisher : Springer Science & Business Media
Page : 449 pages
File Size : 51,8 Mb
Release : 2001-04-20
Category : Medical
ISBN : 9781592591206

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Clinical and Forensic Applications of Capillary Electrophoresis by John R. Petersen,Amin A. Mohammad Pdf

Because capillary electrophoresis (CE) is driving the automation of manual and semi-manual techniques, conserves precious samples, and minimizes the use of hazardous organic chemicals, it constitutes a powerful new methodology for the clinical laboratory. In Clinical and Forensic Applications of Capillary Electrophoresis, John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and nonaqueous electrophoresis. The authors show how a variety of detection methods (UV-visible, fluorescence, and MS) can be used in conjunction with CE to detect a broad array of analytes, ranging from serum proteins to PCR products to drugs of forensic importance. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.

Handbook of Capillary Electrophoresis Applications

H. Shintani

Author : H. Shintani
Publisher : Springer Science & Business Media
Page : 750 pages
File Size : 49,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789400915619

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Handbook of Capillary Electrophoresis Applications by H. Shintani Pdf

Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance. The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science.

Pharmaceutical and Biomedical Applications of Capillary Electrophoresis

S.M. Lunte,D.M. Radzik

Author : S.M. Lunte,D.M. Radzik
Publisher : Elsevier
Page : 508 pages
File Size : 47,9 Mb
Release : 1996-08-13
Category : Science
ISBN : 0080538711

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Pharmaceutical and Biomedical Applications of Capillary Electrophoresis by S.M. Lunte,D.M. Radzik Pdf

The book describes the theory and applications of Capillary Electrophoresis (CE) in the field of pharmaceutical and biomedical analysis. It is targeted towards users who are intimately involved in analytical problems, especially those which involve small samples. This book presents the technique of capillary electrophoresis from the point of view of the serious hands-on use in the field of pharmaceutical and biomedical analysis. An overview of general theory is presented to acquaint the novice with the fundamental principles. A more theoretical approach is taken in the presentation of electrokinetic chromatography. The next chapter discusses advances in column technologies, the preceding chapters having provided a foundation as to how separations occur. In the next three chapters, recognized experts in their fields present fundamentals and state-of-the-art techniques in the areas of optical, electrochemical and mass spectrometric detection. The major focus of the remaining chapters is on applications. This includes the analysis of pharmaceuticals, amino acids and peptides, macromolecules, nucleosides, nucleotides and oligonucleotides. The use of CE for analysis of small ions and separation of biological particles is also discussed. The issue of sample preparation for analysis by CE is addressed, especially as it relates to clinical analysis.

Capillary Electrophoresis Technology

Norberto A. Guzman

Author : Norberto A. Guzman
Publisher : CRC Press
Page : 896 pages
File Size : 40,8 Mb
Release : 1993-08-10
Category : Science
ISBN : 0824790421

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Capillary Electrophoresis Technology by Norberto A. Guzman Pdf

Tracing the sequence of observations that has led to current understanding in the field, this reference presents the basic concepts, instrumentation and applications of capillary electrophoresis, examining its many features such as high-power resolution, high-mass sensitivity, overall sensitivity and low-sample volume requirements. This work highlights the use of capillary elctrophoresis for the identification, separation, detection and characterization of substances on the molecular counting level. Illustrating the major technical maneuvers for common operations and applications, Capillary Electrophoresis Technology outlines the theoretical concepts and mathematical expressions of capillary electrophoresis; describes advances in instrumentation hardware and detection systems. It explains the advantages and limitations of the different variants of capillary electrophoresis; and provides extra coverage of areas in which capillary electrophoresis has grown increasingly popular, including the identification and characterization of small molecules and macromolecules. Written by experts in the field, this book is aimed at analytical and clinical chemists and biochemists, chemical engineers, biologists, pharmacists, biotechnologists and students in these disciplines.

Capillary Gel Electrophoresis

Andras Guttman,László Hajba

Author : Andras Guttman,László Hajba
Publisher : Newnes
Page : 391 pages
File Size : 45,6 Mb
Release : 2021-12-04
Category : Science
ISBN : 9780080931357

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Capillary Gel Electrophoresis by Andras Guttman,László Hajba Pdf

Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Clinical and Forensic Applications of Capillary Electrophoresis. Pathology and Laboratory Medicine

John R. Petersen,Amin A. Mohammad

Author : John R. Petersen,Amin A. Mohammad
Publisher : Unknown
Page : 128 pages
File Size : 40,6 Mb
Release : 2001
Category : Electronic
ISBN : OCLC:748539240

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Clinical and Forensic Applications of Capillary Electrophoresis. Pathology and Laboratory Medicine by John R. Petersen,Amin A. Mohammad Pdf

Because capillary electrophoresis (CE) is driving the automation of manual and semi-manual techniques, conserves precious samples, and minimizes the use of hazardous organic chemicals, it constitutes a powerful new methodology for the clinical laboratory. In Clinical and Forensic Applications of Capillary Electrophoresis, John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and nonaqueous electrophoresis. The authors show how a variety of detection methods (UV-visible, fluorescence, and MS) can be used in conjunction with CE to detect a broad array of analytes, ranging from serum proteins to PCR products to drugs of forensic importance. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.

Handbook of Capillary Electrophoresis, Second Edition

James P. Landers

Author : James P. Landers
Publisher : CRC Press
Page : 918 pages
File Size : 40,8 Mb
Release : 1996-12-23
Category : Medical
ISBN : 084932498X

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Handbook of Capillary Electrophoresis, Second Edition by James P. Landers Pdf

Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.

Capillary Electrophoresis

Patrick Camilleri

Author : Patrick Camilleri
Publisher : CRC Press
Page : 586 pages
File Size : 50,6 Mb
Release : 1997-11-25
Category : Medical
ISBN : 084939127X

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Capillary Electrophoresis by Patrick Camilleri Pdf

The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material. Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.

Capillary Electrophoresis

S.F.Y. Li

Author : S.F.Y. Li
Publisher : Elsevier
Page : 581 pages
File Size : 43,8 Mb
Release : 1992-07-31
Category : Science
ISBN : 0080858600

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Capillary Electrophoresis by S.F.Y. Li Pdf

Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE have been reported. Rapid, reproducible separations of extremely small amounts of chemicals and biochemicals, including peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, vitamins, inorganic ions, pharmaceuticals and environmental pollutants have been demonstrated. A wide range of applications have been developed in greatly diverse fields, such as chemical, biotechnological, environmental and pharmaceutical analysis. This book covers all aspects of CE, from the principles and technical aspects to the most important applications. It is intended to meet the growing need for a thorough and balanced treatment of CE. The book will serve as a comprehensive reference work. Both the experienced analyst and the newcomer will find the text useful.

Capillary Electrophoresis - Mass Spectrometry (CE-MS)

Gerhardus de Jong

Author : Gerhardus de Jong
Publisher : John Wiley & Sons
Page : 364 pages
File Size : 46,6 Mb
Release : 2016-09-13
Category : Science
ISBN : 9783527339242

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Capillary Electrophoresis - Mass Spectrometry (CE-MS) by Gerhardus de Jong Pdf

This monograph offers the reader a complete overview on both principles and applications of CE-MS. Starting with an introductory chapter on detection in CE, also related and more specialized techniques such as electrophoretic and chromatographic preconcentration are discussed. A special emphasis is put on CE-MS interfaces, which are described in detail. In a separate chapter, attention is paid to sheath-liquid interfacing. The developments and possibilities of microchip CE-MS are also described. Applications to all relevant areas are discussed in distinct chapters, each written by experts in the respective fields. Besides applications in pharmaceutical analysis and bioanalysis, recent implementations in food science, forensic analysis, analysis of intact proteins, metabolomics and proteomics are highlighted. MS is a perfectly appropriate detection system for CE, as efficient separation is coupled to sensitive and selection detection. Moreover, MS can provide structure information on the separated compounds. CE-MS has now been developed into a strong hyphenated system complementary to LC-MS. This monograph is an unique source of knowledge for everyone dealing with and interested in CE-MS.

Capillary Electrophoresis of Biomolecules

Nicola Volpi,Francesca Maccari

Author : Nicola Volpi,Francesca Maccari
Publisher : Humana Press
Page : 374 pages
File Size : 46,5 Mb
Release : 2016-05-01
Category : Science
ISBN : 1493962485

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Capillary Electrophoresis of Biomolecules by Nicola Volpi,Francesca Maccari Pdf

This book details key techniques used to investigate Capillary electrophoresis (CE). It focuses on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids.

Capillary Electrophoresis: Principles and Practice

Reinhard Kuhn,Sabrina Hoffstetter-Kuhn

Author : Reinhard Kuhn,Sabrina Hoffstetter-Kuhn
Publisher : Springer Science & Business Media
Page : 381 pages
File Size : 53,9 Mb
Release : 2013-03-07
Category : Science
ISBN : 9783642780585

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Capillary Electrophoresis: Principles and Practice by Reinhard Kuhn,Sabrina Hoffstetter-Kuhn Pdf

Capillary electrophoresis (CE) is a brand-new analytical me- thod with the capability of solving many analytical separa- tion problems very fast and economically. This method gives new information about the investigated substances which can- not easily be obtained by other means. CE has become an established method only recently, but will be implemented in almost every analytical laboratory in industry, service units and academia in the near future. The most important fields of CE application are pharmaceutical and biochemical research and quality control. The authors have exhaustive practical experience in the application of CE methods in the pharmaceutical industry and provide the reader with a comprehensive treatment of this method. The main focus is on how to solve problems when applying CE in the laboratory. Physico-chemical theory is only dealt with in depth when necessary to understand the underlying separation mechanisms in order to solve your problems at the analytical bench. An addendum includes tables on the preparation of buffers and recommended further reading.

Capillary Electromigration Separation Methods

Colin F. Poole

Author : Colin F. Poole
Publisher : Elsevier
Page : 626 pages
File Size : 40,8 Mb
Release : 2018-04-13
Category : Science
ISBN : 9780128096147

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Capillary Electromigration Separation Methods by Colin F. Poole Pdf

Capillary Electromigration Separation Methods is a thorough, encompassing reference that not only defines the concept of contemporary practice, but also demonstrates its implementation in laboratory science. Chapters are authored by recognized experts in the field, ensuring that the content reflects the latest developments in research. Thorough, comprehensive coverage makes this the ideal reference for project planning, and extensive selected referencing facilitates identification of key information. The book defines the concept of contemporary practice in capillary electromigration separation methods, also discussing its applications in small mass ions, stereoisomers, and proteins. Edited and authored by world-leading capillary electrophoresis experts Presents comprehensive coverage on the subject Includes extensive referencing that facilitates the identification of key research developments Provides more than 50 figures and tables that aid in the retention of key concepts

Clinical Applications Of Capillary Electrophoresis

Clinical Applications of Capillary Electrophoresis by Stephen M. Palfrey Pdf

Clinical Applications of Capillary Electrophoresis by Terry M. Phillips,Heather Kalish Pdf

Clinical and Forensic Applications of Capillary Electrophoresis by John R. Petersen,Amin A. Mohammad Pdf

Handbook of Capillary Electrophoresis Applications by H. Shintani Pdf

Pharmaceutical and Biomedical Applications of Capillary Electrophoresis by S.M. Lunte,D.M. Radzik Pdf

Capillary Electrophoresis Technology by Norberto A. Guzman Pdf

Capillary Gel Electrophoresis by Andras Guttman,László Hajba Pdf

Clinical and Forensic Applications of Capillary Electrophoresis. Pathology and Laboratory Medicine by John R. Petersen,Amin A. Mohammad Pdf

Handbook of Capillary Electrophoresis, Second Edition by James P. Landers Pdf

Capillary Electrophoresis by Patrick Camilleri Pdf

Capillary Electrophoresis by S.F.Y. Li Pdf

Capillary Electrophoresis - Mass Spectrometry (CE-MS) by Gerhardus de Jong Pdf

Capillary Electrophoresis of Biomolecules by Nicola Volpi,Francesca Maccari Pdf

Capillary Electrophoresis: Principles and Practice by Reinhard Kuhn,Sabrina Hoffstetter-Kuhn Pdf

Capillary Electromigration Separation Methods by Colin F. Poole Pdf

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