Cell Culture Approaches To Invertebrate Neuroscience

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Cell Culture Approaches to Invertebrate Neuroscience

Author : George Lees,D. Beadle,Stanley B. Kater
Publisher : Unknown
Page : 264 pages
File Size : 51,9 Mb
Release : 1988
Category : Science
ISBN : UOM:39015013218162

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Cell Culture Approaches to Invertebrate Neuroscience by George Lees,D. Beadle,Stanley B. Kater Pdf

Cell Culture in the Neurosciences

Author : Jane Bottenstein
Publisher : Springer Science & Business Media
Page : 392 pages
File Size : 43,8 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461324737

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Cell Culture in the Neurosciences by Jane Bottenstein Pdf

A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.

The Oxford Handbook of Invertebrate Neurobiology

Author : John H. Byrne
Publisher : Oxford University Press
Page : 608 pages
File Size : 50,5 Mb
Release : 2019-01-29
Category : Science
ISBN : 9780190456788

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The Oxford Handbook of Invertebrate Neurobiology by John H. Byrne Pdf

Invertebrates have proven to be extremely useful model systems for gaining insights into the neural and molecular mechanisms of sensory processing, motor control and higher functions such as feeding behavior, learning and memory, navigation, and social behavior. A major factor in their enormous contributions to neuroscience is the relative simplicity of invertebrate nervous systems. In addition, some invertebrates, primarily the molluscs, have large cells, which allow analyses to take place at the level of individually identified neurons. Individual neurons can be surgically removed and assayed for expression of membrane channels, levels of second messengers, protein phosphorylation, and RNA and protein synthesis. Moreover, peptides and nucleotides can be injected into individual neurons. Other invertebrate model systems such as Drosophila and Caenorhabditis elegans offer tremendous advantages for obtaining insights into the neuronal bases of behavior through the application of genetic approaches. The Oxford Handbook of Invertebrate Neurobiology reviews the many neurobiological principles that have emerged from invertebrate analyses, such as motor pattern generation, mechanisms of synaptic transmission, and learning and memory. It also covers general features of the neurobiology of invertebrate circadian rhythms, development, and regeneration and reproduction. Some neurobiological phenomena are species-specific and diverse, especially in the domain of the neuronal control of locomotion and camouflage. Thus, separate chapters are provided on the control of swimming in annelids, crustaea and molluscs, locomotion in hexapods, and camouflage in cephalopods. Unique features of the handbook include chapters that review social behavior and intentionality in invertebrates. A chapter is devoted to summarizing past contributions of invertebrates to the understanding of nervous systems and identifying areas for future studies that will continue to advance that understanding.

Neurons: Methods and Applications for the Cell Biologist

Author : Anonim
Publisher : Elsevier
Page : 500 pages
File Size : 40,5 Mb
Release : 2003-10-02
Category : Science
ISBN : 0080496636

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Neurons: Methods and Applications for the Cell Biologist by Anonim Pdf

Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. Contains culture methodology for more than a dozen types of CNS and PNS neurons Includes most recent and reliable techniques from expert practitioners for specific experimental applications Addresses the latest strategies for transfecting neurons

Nervous System Regeneration in the Invertebrates

Author : Stacia B. Moffet
Publisher : Springer Science & Business Media
Page : 219 pages
File Size : 43,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642798399

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Nervous System Regeneration in the Invertebrates by Stacia B. Moffet Pdf

Invertebrate animals represent a diversity of solutions to life's challenges. Success in a wide range of environments has been achieved by an almost bewildering range of invertebrate body forms. These body forms are reflected in the wonderful diversity of their nervous systems. Despite this apparent diversity, studies of the development of invertebrates and vertebrates are yielding common themes at the molecular level. Likewise, the phenome non of neural regeneration is based upon properties intrinsic to neurons and responses to a remarkably conserved chemical lan guage. This monograph focuses on the diversity and commonal ity of responses to neural injury. The rough and tumble of life may frequently damage some part of the body, particularly the appendages or sensory sys tems. The nervous system is usually involved in repair of other body systems and often may itself require repair. Some animals are particularly successful in regenerating the nervous system or body parts. We particularly marvel at these feats of regeneration because we human beings are not particularly successful, despite our relatively long life and the advantages that would seem to accrue from such repair. It is no wonder that we would hope to learn the secrets of the more successful animals and strive to emulate them! Mechanisms of neural regeneration are often more acces sible in invertebrates than in vertebrates because questions of specificity are more easily addressed using the identifiable neu rons of the relatively simpler nervous systems of some inverte brates.

Insect Cell Biotechnology

Author : Gordon D. O. Maramorosch
Publisher : CRC Press
Page : 200 pages
File Size : 55,8 Mb
Release : 2018-01-18
Category : Science
ISBN : 9781351090476

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Insect Cell Biotechnology by Gordon D. O. Maramorosch Pdf

Insect Cell Biotechnology provides a lucid, up-to-date description of recent major advances in the field. A number of significant topics are addressed, including the use and production of baculoviruses in insect cells, baculovirus specificity, bacterial toxin studies in cultured insect cells, scale-up operations required in the production of recombinant protein and insect viruses propagated in insect cells, growth and nervous system interactions, and the physiological and developmental capacities of cell lines. Transfection in Drosophila cells and a chapter on the theoretical and practical implications of stress produced by x-rays, ultraviolet light, chemicals, psoralens, and heat are discussed as well.

Culturing Nerve Cells

Author : Gary Banker,Kimberly Goslin
Publisher : MIT Press
Page : 706 pages
File Size : 40,5 Mb
Release : 1998
Category : Anatomy
ISBN : 0262024381

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Culturing Nerve Cells by Gary Banker,Kimberly Goslin Pdf

A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.

American Neuroscience in the Twentieth Century

Author : H.W. Magoun,L. Marshall
Publisher : CRC Press
Page : 500 pages
File Size : 54,8 Mb
Release : 2005-08-08
Category : Psychology
ISBN : 9780203970959

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American Neuroscience in the Twentieth Century by H.W. Magoun,L. Marshall Pdf

A history of how neural, behavioural and communicative subdisciplines coalesced in neuroscience to create a promising approach to understanding the relation of mind to brain. It chronicles the expansion of prominent centres of research and the development of innovative apparatus and concepts.

International Review of Neurobiology

Author : Anonim
Publisher : Academic Press
Page : 370 pages
File Size : 40,9 Mb
Release : 1991-01-02
Category : Medical
ISBN : 0080857663

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International Review of Neurobiology by Anonim Pdf

International Review of Neurobiology

International Review of Cytology

Author : Anonim
Publisher : Academic Press
Page : 305 pages
File Size : 49,6 Mb
Release : 1994-08-03
Category : Science
ISBN : 0080856918

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International Review of Cytology by Anonim Pdf

International Review of Cytology presents current advances and comprehensive reviews in cell biology-both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research.

Methods in Neurobiology

Author : Robert Lahue
Publisher : Springer Science & Business Media
Page : 607 pages
File Size : 52,6 Mb
Release : 2013-11-21
Category : Medical
ISBN : 9781468438062

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Methods in Neurobiology by Robert Lahue Pdf

Rapid advances in knowledge have led to an increasing interest in neuro biology over the last several years. These advances have been made possible, at least in part, by the use of increasingly sophisticated methodology. Furthermore, research in the most rapidly advancing areas is essentially multidisciplinary and is characterized by contributions from many investi gators employing a variety of techniques. While a grasp of fundamental neurobiological concepts is an obvious prerequisite for those who wish to follow or participate in this field, critical awareness and evaluation of neurobiological research also requires an understanding of sophisticated methodologies. The objective of Methods in Neurobiology is the development of such critical abilities. The reader is exposed to the basic concepts, principles, and instrumentation of key methodologies, and the application of each meth odology is placed in the special context of neurobiological research. The reader will gain familiarity with the terminology and procedures of each method and the ability to evaluate results in light of the particular features of neurobiological preparations and applications.

Guide to Research Techniques in Neuroscience

Author : Matt Carter,Rachel Essner,Nitsan Goldstein,Manasi Iyer
Publisher : Academic Press
Page : 416 pages
File Size : 45,8 Mb
Release : 2022-03-26
Category : Medical
ISBN : 9780323915618

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Guide to Research Techniques in Neuroscience by Matt Carter,Rachel Essner,Nitsan Goldstein,Manasi Iyer Pdf

Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step

Advances in Neural Science

Author : S.K. Malhotra
Publisher : Elsevier
Page : 256 pages
File Size : 50,7 Mb
Release : 1995-12-08
Category : Science
ISBN : 0080526403

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Advances in Neural Science by S.K. Malhotra Pdf

Part of a series which aims to cover recent knowledge in the field of neural science, this volume discusses such topics as: the molecular bases of nerve regeneration; plasticity of descending spinal pathways in developing mammals; and development of the mammalian auditory hindbrain.

Molecular Approaches to Neurobiology

Author : Ian R. Brown
Publisher : Academic Press
Page : 434 pages
File Size : 43,7 Mb
Release : 2014-06-28
Category : Science
ISBN : 9781483282299

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Molecular Approaches to Neurobiology by Ian R. Brown Pdf

Molecular Approaches to Neurobiology deals with molecular approaches to the analysis of the nervous system. This book surveys the current state of knowledge in a number of areas of molecular neurobiology that includes the subcellular level of chromatin structure, complexity of RNA synthesis, role of hormones in cell differentiation, and molecular correlates between neuropeptides and behavior. A comprehensive review of procedures for the isolation of specific brain cells and their experimental use is also provided at the end of this text. This publication is useful to those working in the field of neurochemistry and those engaged in morphological and physiological approaches to the analysis of the nervous system, as well as molecular biologists and biochemists working with non-neural tissues.

Drosophila Cells in Culture

Author : Guy Echalier
Publisher : Academic Press
Page : 702 pages
File Size : 40,8 Mb
Release : 1997-02-24
Category : Science
ISBN : 0080530532

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Drosophila Cells in Culture by Guy Echalier Pdf

Currently Drosophila is a dominant experimental model in developmental biology and in gene regulation in eukaryotes. This volume summarizes some thirty years of experience in the handling of in vitro cultured Drosophila cells. Its main emphasis is on gene transfer methodology, cell responses to heat shock, hormonal regulation of genes, and on the expression and mobility of transposable elements. Some thirty years of experience in handling in vitro cultured Drosophila cells Cell cultures which provide material for a multiplicity of biochemical approaches DNA-mediated gene transfer as an irreplaceable tool for analyzing basic mechanisms of regulation Drosophila cell lines which qualify them for use in biotechnology