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Author : Gary Banker,Kimberly Goslin
Publisher : MIT Press
Page : 706 pages
File Size : 55,8 Mb
Release : 1998
Category : Anatomy
ISBN : 0262024381
A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.
Author : Shohreh Amini,Martyn K. White
Publisher : Humana
Page : 205 pages
File Size : 41,6 Mb
Release : 2022-06-09
Category : Medical
ISBN : 1071614398
This second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies.
Author : Alan L. Harvey
Publisher : Springer Science & Business Media
Page : 317 pages
File Size : 41,8 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781468488104
The techniques of tissue culture were introduced at the beginning of this century. They have become more and more popular as it is realized that they are not as difficult or as esoteric as some early protagonists liked to maintain. Most of the work performed with culture methods has simply concerned cell growth and survival. Biologists have long used culture approaches to provide a simple system in which to study cell division and multiplication. Any pharmacology done on cultured tissue was largely toxicological or as part of a screening programme for poten tial anti-cancer drugs. In the last decade there has been a great increase in the use of excitable cells in tissue culture. Nerves and muscles from a wide variety of sources can maintain their highly differentiated properties in culture. Such cultures offer an attractive preparation for use in physiological and pharmacological investigations. Consequently, a vast amount of work has been produced, and this book is an attempt to review it. It is hoped that this will introduce physiologists and pharmacologists to the potential of culture methods for their experiments and also indicate to more traditional tissue culture users further possible areas of interest. By being more comprehensive in scope and by trying to concentrate largely on drug actions, I hope that the present volume usefully extends the treatment of the subject begun earlier in the excellent works by Crain (1976) and Nelson and Lieberman (1981).
Author : Jane Bottenstein
Publisher : Springer Science & Business Media
Page : 392 pages
File Size : 54,7 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461324737
A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.
Author : Marilyn Anderson Ludueña
Publisher : Unknown
Page : 198 pages
File Size : 46,9 Mb
Release : 1972
Category : Cell culture
ISBN : STANFORD:36105025653895
Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 54,8 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181
Author : David Schubert
Publisher : Wiley-Interscience
Page : 344 pages
File Size : 44,7 Mb
Release : 1984
Category : Medical
ISBN : UOM:39015006031580
Author : Irwin B. Levitan,Leonard K. Kaczmarek
Publisher : Oxford University Press, USA
Page : 640 pages
File Size : 49,8 Mb
Release : 2002
Category : Molecular neurobiology
ISBN : 0195145232
Intended for use by advanced undergraduate, graduate and medical students, this book presents a study of the unique biochemical and physiological properties of neurons, emphasising the molecular mechanisms that generate and regulate their activity.
Author : Sergey Fedoroff,Arleen Richardson
Publisher : Springer Science & Business Media
Page : 284 pages
File Size : 46,5 Mb
Release : 2013-04-17
Category : Medical
ISBN : 9781475725865
This volume is designed to be kept close at hand as a ready reference and a guide to laboratory procedures. It is based on tissue culture manuals used for a number of years at international courses on tissue culture at the University of Saskatchewan, made possible by the generous support of the Canadian Council of Animal Care and the Medical Research Council of Canada. Sergey Fedoroff Arleen Richardson The second edition of Protocols for Neural Cell Culture adheres to the prin ciples enunciated in the first edition, but the content has been extensively revised and expanded. Two new chapters have been added to reflect the increased interest in the development and differentiation of the nervous system and in the reconstruction of its circuitry in tissue culture. One chapter deals with slice cul tures in which the organization of the nervous system is preserved. When slice cultures are combined with explant cultures, afferent and efferent projections can be reconstructed. The other chapter deals with aggregating neural cell cul tures, in which "minibrains" can form. Theses are small, uniformly sized spheres of nervous tissue, usually having nerve cells in the center and astrocytes, oligo dendrocytes, and microglia in the periphery. Such cultures can be used to study neutral cell interactions in an organized milieu and for qualitative as well as quantitative studies at biochemical and molecular levels.
Author : Gordon Sato
Publisher : Springer Science & Business Media
Page : 298 pages
File Size : 48,6 Mb
Release : 2013-06-29
Category : Medical
ISBN : 9781468429046
The impetus for compiling this book was the recent development of culture strains of neuroblastoma and glial cells and the immediate and enthusiastic way they have been taken up as model systems. After the first sudden rush of activity, it seems appropriate to pause, to assess progress, and to contemplate the future contributions that may be possible using these culture techniques. Long before the advent of established strains, cultures of nervous tissue had already contributed to neurobiology. Ross Harrison, in 1906, in a single experimental series, established tissue culture as a promising new technique in cell biology and settled the Golgi-Cajal controversy as to whether axonic processes originated as outgrowths from the cell body or were formed first in the intercellular spaces and were later connected to the cell body. Harrison observed process growth from nerve cells in cultures, thus settling the matter in favor of Cajal. Of great importance to neurobiology is the discovery by Rita Levi-Montalcini of nerve growth factor. Cultures of spinal ganglia played a major role in the discovery, isolation, and characterization of the factor (Levi-Montalcini et ai. , 1954). In my opinion, this discovery, although very well known, has not yet been adequately recognized for its germinal influence on neurobiology and embryology. Progress since the advent of clonal cultures has been more modest. I would like to cite two pieces of work which emphasize the technical ad vantages of these cultures.
Author : Peter Riederer,Heinz Reichmann,Moussa B. H. Youdim,Manfred Gerlach
Publisher : Springer Science & Business Media
Page : 484 pages
File Size : 42,6 Mb
Release : 2006-11-23
Category : Medical
ISBN : 9783211452950
This book gives a comprehensive overview on current clinical and basic research issues related to Parkinson’s disease and its related disorders. The book is the result of the 16th International Congress of Parkinson’s Disease and Related disorders congress 2005. Its contents are suitable for neurologists, psychiatrists, neurosurgeons, basic researchers, geneticians and patients as well as their relatives.
Author : William M. Reichert
Publisher : CRC Press
Page : 300 pages
File Size : 54,7 Mb
Release : 2007-12-17
Category : Medical
ISBN : 9781420009309
Despite enormous advances made in the development of external effector prosthetics over the last quarter century, significant questions remain, especially those concerning signal degradation that occurs with chronically implanted neuroelectrodes. Offering contributions from pioneering researchers in neuroprosthetics and tissue repair, Indwel
Author : Steven E. Pfeiffer
Publisher : Springer
Page : 256 pages
File Size : 42,6 Mb
Release : 1982-08-30
Category : Medical
ISBN : UOM:39015010062233
Author : Shohreh Amini,Martyn K. White
Publisher : Unknown
Page : 165 pages
File Size : 46,6 Mb
Release : 2013
Category : Cell culture
ISBN : 1627036407
In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.
Author : Makoto Taketani,Michel Baudry
Publisher : Springer Science & Business Media
Page : 488 pages
File Size : 41,6 Mb
Release : 2006-11-22
Category : Medical
ISBN : 9780387258584
Advances in Network Electrophysiology: Using Multi Electrode Arrays explores methods for using electrophysiological techniques for monitoring the concurrent activity of ensembles of single neurons. It reviews the recent progress in both electronics and computational tools developed to analyze the functional operations of large ensembles of neurons using multi-electrode arrays and in vitro preparations. In addition, it gives readers a sense of the applications made possible by these technological tools. This volume is the reference for researchers, industry, graduate students, and postdoctoral fellows in all areas of neuroscience, cognitive neuroscience, pharmaceutical science, and bioengineering.
