Cell Culture In The Neurosciences

Author : Jane Bottenstein
Publisher : Springer Science & Business Media
Page : 392 pages
File Size : 41,7 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461324737

Get Book

Cell Culture in the Neurosciences by Jane Bottenstein Pdf

A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.

Author : Steven E. Pfeiffer
Publisher : Springer
Page : 256 pages
File Size : 52,7 Mb
Release : 1982-08-30
Category : Medical
ISBN : UOM:39015010062233

Get Book

Neuroscience Approached Thru Cell Culture by Steven E. Pfeiffer Pdf

Author : Steven E. Pfeiffer
Publisher : Unknown
Page : 208 pages
File Size : 53,8 Mb
Release : 1982
Category : Cell culture
ISBN : UOM:39015000785645

Get Book

Neuroscience Approached Through Cell Culture by Steven E. Pfeiffer Pdf

Author : Jane Bottenstein
Publisher : Unknown
Page : 408 pages
File Size : 41,8 Mb
Release : 1985-05-01
Category : Electronic
ISBN : 1461324742

Get Book

Cell Culture in the Neurosciences by Jane Bottenstein Pdf

Author : Sergey Fedoroff,Arleen Richardson
Publisher : Springer Science & Business Media
Page : 366 pages
File Size : 50,7 Mb
Release : 2008-06-29
Category : Medical
ISBN : 9781592592074

Get Book

Protocols for Neural Cell Culture by Sergey Fedoroff,Arleen Richardson Pdf

The first edition of Protocols for Neural Cell Culture was published in 1992 and the second edition in 1997. Originally, the publication grew outofprotocols used in the Tissue Culture Course given at the University of Saskatchewan. The course was patterned on those given by the Tissue CultureAssociation, first in Toronto, Canada, in 1948, then in Cooperstown, NY, then Denver, CO, and finally in Madison, WI, where the course ended in 1964. The course in Saskatchewan began in 1963 as a month-long international course that included both animal and plant tissue cultures. Over the years the course underwent specialization, first being limited to animal tissue culture, then to an intensive one-week general course. This led to one-week courses especially designed for tissue culture for the study of cancer or of the cardiovascular or the nervous system. In 1989, the Saskatchewan course became part of the Tissue Culture Training Facility of the Neuroscience Network of the Canadian Network of Centres of Excellence. The course and the Training Facility ceased to exist in 1997. The faculty for the Saskatchewan course was drawn from the best labora tories in the world and laboratory protocols from those centers were thoroughly tested in a student laboratory setting for many years.

Author : P. Michael Conn
Publisher : Unknown
Page : 454 pages
File Size : 43,6 Mb
Release : 1990
Category : Science
ISBN : UVA:X001810849

Get Book

Cell Culture by P. Michael Conn Pdf

The ability to assess the response of neural cells is an important goal of research in the neurosciences. The means to measure responses in a chemically defined medium is a positive feature of the use of cell culture and is the major reason its use is so important in this discipline. Techniques for the purification of cells for culture, for large-scale isolation, for assessing differentiated function, and for tracing lineage are included. Many of the methods can easily be adapted to other systems. Key Features * Convenient benchtop format * Methods presented for easy adaptation to new systems * Comprehensive protocols included for * Preparation and maintenance of primary and continuous cultures * Cell purification * Bulk isolation * Studying pulsatility, growth on immobile carriers, rotation-mediated aggregation, microcultures, Maximow assembly cultures * Assessment of differentiated and functional aspects: endocrine modulation, morphological variants, receptors, intracellular trafficking * Clonal analysis and lineage analysis

Author : S. Fedoroff
Publisher : Elsevier
Page : 708 pages
File Size : 45,5 Mb
Release : 2012-12-02
Category : Nature
ISBN : 9780323143158

Get Book

Cell, Tissue, and Organ Cultures in Neurobiology by S. Fedoroff Pdf

Cell, Tissue, and Organ Cultures in Neurobiology emerged from an international workshop held at the University of Saskatchewan in March 1977. This book reviews the uses of cell, tissue, and organ cultures in neurobiological research. It brings together an interdisciplinary perspective from morphology, biochemistry, pharmacology, endocrinology, embryology, and genetics. The book is organized into seven parts. Part I contains papers on the characteristics of differentiated cells. Part II presents studies on cell differentiation in primary cultures. Part III deals with studies on cell cultures and cell strains. Part IV focuses on phenotypic cell expression. Part V examines various cellular interactions. Part VI covers studies on nutrition while Part VII takes up applications of cell tissue and organ cultures in neurobiology. The book is directed toward tissue culturists concerned with the nervous system, as well as all neurobiologists, cell biologists, and embryologists interested in learning how neural cells and tissues behave in cultures and what has been learned about the nervous system using tissue culture methods, including the applicability of tissue cultures to the study of cell differentiation.

Author : Gary Banker,Kimberly Goslin
Publisher : MIT Press
Page : 706 pages
File Size : 40,7 Mb
Release : 1998
Category : Anatomy
ISBN : 0262024381

Get Book

Culturing Nerve Cells by Gary Banker,Kimberly Goslin Pdf

A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols.

Author : Alan A. Boulton,Glen B. Baker,Wolfgang Walz
Publisher : Humana Press
Page : 392 pages
File Size : 47,6 Mb
Release : 1992-09-09
Category : Medical
ISBN : 0896033481

Get Book

Practical Cell Culture Techniques by Alan A. Boulton,Glen B. Baker,Wolfgang Walz Pdf

Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.

Author : George Lees,D. Beadle,Stanley B. Kater
Publisher : Unknown
Page : 264 pages
File Size : 45,6 Mb
Release : 1988
Category : Science
ISBN : UOM:39015013218162

Get Book

Cell Culture Approaches to Invertebrate Neuroscience by George Lees,D. Beadle,Stanley B. Kater Pdf

Author : Adalberto Merighi,Giorgio Carmignoto
Publisher : Springer Science & Business Media
Page : 307 pages
File Size : 54,7 Mb
Release : 2007-12-14
Category : Medical
ISBN : 9780387224602

Get Book

Cellular and Molecular Methods in Neuroscience Research by Adalberto Merighi,Giorgio Carmignoto Pdf

There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.

Author : Shohreh Amini,Martyn K. White
Publisher : Humana Press
Page : 165 pages
File Size : 46,5 Mb
Release : 2013-08-24
Category : Medical
ISBN : 1627036415

Get Book

Neuronal Cell Culture by Shohreh Amini,Martyn K. White Pdf

In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.

Author : Ezio Giacobini,Antonia Vernadakis,Abraham Shahar
Publisher : Unknown
Page : 544 pages
File Size : 40,6 Mb
Release : 1980
Category : Acetylcholine
ISBN : UCAL:B4524735

Get Book

Tissue Culture in Neurobiology by Ezio Giacobini,Antonia Vernadakis,Abraham Shahar Pdf

Author : Michael Aschner,Lucio Costa
Publisher : Humana Press
Page : 302 pages
File Size : 47,8 Mb
Release : 2019-06-01
Category : Medical
ISBN : 1493992279

Get Book

Cell Culture Techniques by Michael Aschner,Lucio Costa Pdf

This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.

Author : Steven E Pfeiffer
Publisher : CRC Press
Page : 184 pages
File Size : 40,6 Mb
Release : 2022-01-12
Category : Electronic
ISBN : 0367260751

Get Book

Neuroscience Approached Through Cell Culture by Steven E Pfeiffer Pdf

First published in 1983: This collection of papers, presented in two volumes, attempts to portray in a non-comprehensive manner some of the areas of current neurobiological research n which culture techniques have made, or now appear to have the potential to soon make, contributions to the questions being posed.