Capillary Electromigration Separation Methods

Author : Colin F. Poole
Publisher : Elsevier
Page : 626 pages
File Size : 52,8 Mb
Release : 2018-04-13
Category : Science
ISBN : 9780128096147

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Capillary Electromigration Separation Methods by Colin F. Poole Pdf

Capillary Electromigration Separation Methods is a thorough, encompassing reference that not only defines the concept of contemporary practice, but also demonstrates its implementation in laboratory science. Chapters are authored by recognized experts in the field, ensuring that the content reflects the latest developments in research. Thorough, comprehensive coverage makes this the ideal reference for project planning, and extensive selected referencing facilitates identification of key information. The book defines the concept of contemporary practice in capillary electromigration separation methods, also discussing its applications in small mass ions, stereoisomers, and proteins. Edited and authored by world-leading capillary electrophoresis experts Presents comprehensive coverage on the subject Includes extensive referencing that facilitates the identification of key research developments Provides more than 50 figures and tables that aid in the retention of key concepts

Author : Lauro Tatsuo Kubota,José Alberto Fracassi da Silva,Marcelo Martins Sena,Wendel Andrade Alves
Publisher : Springer Nature
Page : 559 pages
File Size : 55,9 Mb
Release : 2021-11-25
Category : Science
ISBN : 9783030823818

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Tools and Trends in Bioanalytical Chemistry by Lauro Tatsuo Kubota,José Alberto Fracassi da Silva,Marcelo Martins Sena,Wendel Andrade Alves Pdf

This textbook covers the main tools and techniques used in bioanalysis, provides an overview of their principles, and offers several examples of their application and future trends in diagnosis. Chapters from expert contributors explore the role of bioanalysis in different areas such as biochemistry, physiology, forensics, and clinical diagnosis, including topics from sampling/sample preparation, chemometrics in bioanalysis to the latest techniques used in the field. Particular attention is given to the recent advances in the application of mass spectrometry, NMR, electrochemical methods and separation techniques in bioanalysis. Readers will also find more about the application of microchip-based devices and analytical microarrays. This textbook will appeal to graduate/advanced undergraduate students in Chemistry, Biology, Biochemistry, Pharmacy, and Chemical Engineering. It is also a useful resource for researchers and professionals working in the fields of biomedicine and veterinary sciences, with clear explanations and examples of how the different bioanalytical devices are applied for clinical diagnosis.

Author : Boguslaw Buszewski,Ewelina Dziubakiewicz,Michal Szumski
Publisher : Springer Science & Business Media
Page : 368 pages
File Size : 43,8 Mb
Release : 2013-04-23
Category : Technology & Engineering
ISBN : 9783642350436

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Electromigration Techniques by Boguslaw Buszewski,Ewelina Dziubakiewicz,Michal Szumski Pdf

The book provides the broad knowledge on electromigration techniques including: theory of CE, description of instrumentation, theory and practice in micellar electrokinetic chromatography, isotachophoresis, capillary isoelectric focusing, capillary and planar electrochromatography (including description of instrumentation and packed and monolithic column preparation), 2D-gel electrophoresis (including sample preparation) and lab-on-a-chip systems. The book also provides the most recent examples of applications including food, environmental, pharmaceutical analysis as well as proteomics.

Author : Andras Guttman,László Hajba
Publisher : Newnes
Page : 391 pages
File Size : 48,9 Mb
Release : 2021-12-04
Category : Science
ISBN : 9780080931357

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Capillary Gel Electrophoresis by Andras Guttman,László Hajba Pdf

Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Author : C. F. Poole
Publisher : Elsevier
Page : 952 pages
File Size : 54,8 Mb
Release : 2003
Category : Science
ISBN : 0444501991

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The Essence of Chromatography by C. F. Poole Pdf

The Essence of Chromatography presents a comprehensive survey of modern chromatography and is intended as a suitable text for graduate level courses in the separation sciences and as a self-study guide for professional chromatographers wishing to refresh their background in this rapidly expanding field.This title is an effective replacement for Chromatography Today, written by the same author with Salwa K. Poole, which is considered to be one of the definitive chromatographic texts of the last decade. Its format is modular, with extensive cross-references to permit rapid location of re.

Author : Andrea Weston,Phyllis R. Brown
Publisher : Elsevier
Page : 280 pages
File Size : 45,7 Mb
Release : 1997-07-21
Category : Science
ISBN : 0080534163

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High Performance Liquid Chromatography & Capillary Electrophoresis by Andrea Weston,Phyllis R. Brown Pdf

HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity. Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques Applicable to a wide variety of separation problems Covers basic concepts governing any separation as well as instrumentation and how to use it Helps the user to obtain optimal resolution in minimal time Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation Includes information on upcoming trends such as miniaturization Major concepts in each chapter are organized to allow access to information easily and quickly Contains practical bibliography for accessing the literature

Author : Anonim
Publisher : Elsevier
Page : 770 pages
File Size : 43,7 Mb
Release : 2022-07-22
Category : Science
ISBN : 9780323854870

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Particle Separation Techniques by Anonim Pdf

Particle Separation Techniques: Fundamentals, Instrumentation, and Selected Applications presents the latest research in the field of particle separation methods. This edited book authored by subject specialists is logically organized in sections, grouping the separation techniques according to their preparative or analytical purposes and the particle type. Along with the traditional and classical separation methods suitable for micronic particles, an update survey of techniques appropriate for nanoparticle characterization is presented. This book fills the gap in the literature of particle suspension analysis of a synthetic but comprehensive manual, helping the reader to identify and apply selected techniques.It provides an overview of the techniques available to a reader who is not an expert on particle separation yet about to enter the field, design an experiment, or buy an instrument for his/her new lab. Presents a resource that is ideal for anyone preparing samples across a variety of fields, including pharmaceuticals, food science, pollution analysis and control, agricultural products, and more Includes real case examples discussed by leading experts in the field Provides chapters that contain a unique, common table that summarizes points-of-strength and the weaknesses of each technique

Author : Carlos D. García,Karin Y. Chumbimuni-Torres,Emanuel Carrilho
Publisher : John Wiley & Sons
Page : 638 pages
File Size : 55,8 Mb
Release : 2013-02-01
Category : Science
ISBN : 9781118529997

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Capillary Electrophoresis and Microchip Capillary Electrophoresis by Carlos D. García,Karin Y. Chumbimuni-Torres,Emanuel Carrilho Pdf

Explores the benefits and limitations of the latest capillary electrophoresis techniques Capillary electrophoresis and microchip capillary electrophoresis are powerful analytical tools that are particularly suited for separating and analyzing biomolecules. In comparison with traditional analytical techniques, capillary electrophoresis and microchip capillary electrophoresis offer the benefits of speed, small sample and solvent consumption, low cost, and the possibility of miniaturization. With contributions from a team of leading analytical scientists, Capillary Electrophoresis and Microchip Capillary Electrophoresis explains how researchers can take full advantage of all the latest techniques, emphasizing applications in which capillary electrophoresis has proven superiority over other analytical approaches. The authors not only explore the benefits of each technique, but also the limitations, enabling readers to choose the most appropriate technique to analyze a particular sample. The book's twenty-one chapters explore fundamental aspects of electrophoretically driven separations, instrumentation, sampling techniques, separation modes, detection systems, optimization strategies for method development, and applications. Specific topics include: Critical evaluation of the use of surfactants in capillary electrophoresis Sampling and quantitative analysis in capillary electrophoresis Capillary electrophoresis with electrochemical detection Overcoming challenges in using microchip electrophoresis for extended monitoring applications Capillary electrophoresis of intact unfractionated heparin and related impurities Microchip capillary electrophoresis for in situ planetary exploration Each chapter begins with an introduction and ends with conclusions as well as references to the primary literature. Novices to the field will find this book an easy-to-follow introduction to core capillary electrophoresis techniques and methods. More experienced investigators can turn to the book for troubleshooting tips and expert advice to guide them through the most advanced applications.

Author : Ana Valéria Colnaghi Simionato
Publisher : Springer Nature
Page : 277 pages
File Size : 42,7 Mb
Release : 2021-10-09
Category : Science
ISBN : 9783030772529

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Separation Techniques Applied to Omics Sciences by Ana Valéria Colnaghi Simionato Pdf

This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one.

Author : Robert Weinberger
Publisher : Elsevier
Page : 425 pages
File Size : 51,5 Mb
Release : 2000-04-18
Category : Science
ISBN : 9780080539348

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Practical Capillary Electrophoresis by Robert Weinberger Pdf

In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry. Illustrated with detailed diagrams of electrophoretic phenomena Offers step-by-step methods development schemes Presents techniques for developing quantitative, robust, and precise methods Includes an extensive troubleshooting guide Updates and greatly expands on the first edition-more than 50% of the text is new Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE

Author : Satinder Ahuja
Publisher : John Wiley & Sons
Page : 474 pages
File Size : 41,6 Mb
Release : 2011-03-31
Category : Science
ISBN : 9781118097755

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Chiral Separation Methods for Pharmaceutical and Biotechnological Products by Satinder Ahuja Pdf

Discusses chiral separations and offers guidance for selecting the optimum method for desired results Chiral separations represent the most intriguing and, by some measures, most difficultseparations of chemical compounds. This book provides researchers and students an under-standing of chiral separations and offers a convenient route to selecting the best separation method, saving considerable time and cost in product development. Considering chiral separations in the biotechnological and pharmaceutical industries, as well as for food applications, Dr. Ahuja provides insights into a broad range of topics. Opening with a broad overview of chiral separations, regulatory considerations in drug product development, and basic issues in method development, the book: Covers a variety of modern methods such as gas chromatography, high performance liquid chromatography, supercritical fluid chromatography, and capillary electrophoresis Deals with the impact of chirality on the biological activity of small and large molecules Provides detailed information on useful chiral stationary phases (CSPs) for HPLC Includes handy information on selection of an appropriate CSP, including mechanistic studies Offers strategies for fast method development with HPLC, SFC, and CE Discusses preparatory methods utilized in the pharmaceutical industry With in-depth discussions of the current state of the field as well as suggestions to assist future developments, Chiral Separation Methods for Pharmaceutical and Biotechnological Products is an essential text for laboratory investigators, managers, and regulators who are involved in chiral separations in the pharmaceutical industry, as well as students preparing for careers in these fields.

Author : Ganapathy Subramanian
Publisher : John Wiley & Sons
Page : 641 pages
File Size : 52,6 Mb
Release : 2008-01-08
Category : Science
ISBN : 9783527611720

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Chiral Separation Techniques by Ganapathy Subramanian Pdf

Thoroughly revised, with either entirely new or completely updated contents, this is a practical manual for the small and large-scale preparation of enantiomerically pure products. The result is a vital resource for meeting the highest purity standards in the manufacture of chiral pharmaceuticals, food additives and related compounds. All the approaches covered here are highly relevant to modern manufacturing and quality control schemes in the pharmaceutical and biotech industries, addressing the increasingly important issue of drug safety in view of tougher regulatory standards worldwide.

Author : John P. Cherry,Robert A. Barford
Publisher : The American Oil Chemists Society
Page : 286 pages
File Size : 45,6 Mb
Release : 1988
Category : Science
ISBN : 0935315195

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Methods for Protein Analysis by John P. Cherry,Robert A. Barford Pdf

Author : Anonim
Publisher : Elsevier
Page : 5142 pages
File Size : 42,9 Mb
Release : 2019-04-02
Category : Science
ISBN : 9780081019849

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Encyclopedia of Analytical Science by Anonim Pdf

The third edition of the Encyclopedia of Analytical Science, Ten Volume Set is a definitive collection of articles covering the latest technologies in application areas such as medicine, environmental science, food science and geology. Meticulously organized, clearly written and fully interdisciplinary, the Encyclopedia of Analytical Science, Ten Volume Set provides foundational knowledge across the scope of modern analytical chemistry, linking fundamental topics with the latest methodologies. Articles will cover three broad areas: analytical techniques (e.g., mass spectrometry, liquid chromatography, atomic spectrometry); areas of application (e.g., forensic, environmental and clinical); and analytes (e.g., arsenic, nucleic acids and polycyclic aromatic hydrocarbons), providing a one-stop resource for analytical scientists. Offers readers a one-stop resource with access to information across the entire scope of modern analytical science Presents articles split into three broad areas: analytical techniques, areas of application and and analytes, creating an ideal resource for students, researchers and professionals Provides concise and accessible information that is ideal for non-specialists and readers from undergraduate levels and higher

Author : Jared Anderson,Alain Berthod,Veronica Pino,Apryll M. Stalcup
Publisher : John Wiley & Sons
Page : 2148 pages
File Size : 51,6 Mb
Release : 2016-02-29
Category : Science
ISBN : 9783527333745

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Analytical Separation Science, 5 Volume Set by Jared Anderson,Alain Berthod,Veronica Pino,Apryll M. Stalcup Pdf

Leading the way for analytical chemists developing new techniques. This new comprehensive 5 volume set on separation science provides a much needed research-level text for both academic users and researchers who are working with and developing the most current methods, as well as serving as a valuable resource for graduate and post-graduate students. Comprising of five topical volumes it provides a comprehensive overview of the subject, highlighting aspects that will drive research in this field in the years to come. Volume 1: Liquid Chromatography Volume 2: Special Liquid Chromatography Modes and Capillary Electromigration Techniques Volume 3: Gas, Supercritical and Chiral Chromatography Volume 4: Chromatographic and Related Techniques Volume 5: Sample Treatment, Method Validation, and Applications Key Features: - Comprises over 2,100 pages in 5 volumes – available in print and online - Edited by an international editorial team which has both prominent and experienced senior researchers as well as young and dynamic rising stars - Individual chapters are labeled as either introductory or advanced, in order to guide readers in finding the content at the appropriate level - Fully indexed with cross referencing within and between all 5 volumes