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Author : PHILIP IFESINACHI ANOCHIE
Publisher : Lulu.com
Page : 208 pages
File Size : 46,6 Mb
Release : 2024-06-22
Category : Electronic
ISBN : 9780359870028
Author : Prakash Singh Bisen
Publisher : CRC Press
Page : 1772 pages
File Size : 51,8 Mb
Release : 2014-02-26
Category : Medical
ISBN : 9781466553156
As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.
Author : Sandeep Singh,Dhiraj Kumar
Publisher : Bentham Science Publishers
Page : 186 pages
File Size : 53,5 Mb
Release : 2020-01-23
Category : Science
ISBN : 9789811439292
Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).
Author : Peter Hu,Madhuri Hegde,Patrick Alan Lennon
Publisher : Springer Science & Business Media
Page : 436 pages
File Size : 51,5 Mb
Release : 2012-05-11
Category : Medical
ISBN : 9781461421702
This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today’s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure. It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice. Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.
Author : Peter Hu,Madhuri Hegde,Patrick Alan Lennon
Publisher : Springer
Page : 436 pages
File Size : 50,6 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493941488
This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today’s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure. It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice. Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.
Author : Frederick M. Ausubel
Publisher : Unknown
Page : 0 pages
File Size : 49,6 Mb
Release : 1987
Category : Molecular biology
ISBN : 0471529079
Author : Bimal D. M. Theophilus,Ralph Rapley
Publisher : Springer Science & Business Media
Page : 214 pages
File Size : 42,6 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592592739
1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.
Author : Christina E. Day
Publisher : Humana
Page : 0 pages
File Size : 54,8 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493954822
Histopathology: Methods and Protocols provides a comprehensive guide to the current issues in histopathology. With chapters on organ-based approaches with specific protocols for morphologic, molecular examination and pathological observations governing the therapeutic management of the diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Histopathology: Methods and Protocols seeks to be a useful reference for pathologists, pathology residents and fellows as well as to the clinicians and scientists.
Author : Frederick M. Ausubel
Publisher : Unknown
Page : 128 pages
File Size : 51,5 Mb
Release : 2003-05-01
Category : Electronic
ISBN : 0555012395
Author : William B. Coleman,Gregory J. Tsongalis
Publisher : Springer Science & Business Media
Page : 567 pages
File Size : 43,6 Mb
Release : 2007-10-28
Category : Medical
ISBN : 9781592599288
Accompanying CD-ROM contains ... "a companion eBook version of Molecular diagnostics : for the clinical laboratorian, Second edition ... for downloading and use in the reader's PC or PDA."--Page 4 of cover.
Author : John M. Walker,Ralph Rapley
Publisher : Humana Press
Page : 1124 pages
File Size : 40,5 Mb
Release : 2008-08-25
Category : Science
ISBN : 1603273700
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Author : Sean R. Gallagher,Emily A. Wiley
Publisher : John Wiley & Sons
Page : 679 pages
File Size : 53,8 Mb
Release : 2012-03-19
Category : Science
ISBN : 9780470942413
The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.
Author : Virginia Espina
Publisher : Humana
Page : 414 pages
File Size : 52,6 Mb
Release : 2018-06-09
Category : Science
ISBN : 1493983601
This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from discovery to clinical trial validation and commercial public benefit. Chapters aim to cover the full spectrum of molecular profiling from tumor staging and grading through biomarker discovery, to commercialization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Profiling: Methods and Protocols, Second Edition aims to be a useful guide on molecular profiling.
Author : Frederick M. Ausubel
Publisher : Current Protocols
Page : 924 pages
File Size : 44,8 Mb
Release : 1995-10-06
Category : Medical
ISBN : UOM:39015037279778
A desktop companion to the three-volume Current Protocols in Molecular Biology, the recognized leader in bioscience laboratory manuals. This edition contains over 220 protocols from leading laboratories worldwide. All methods are lab-tested and include step-by-step instructions, equipment and materials necessary to successfully conduct an experiment.
Author : Bruce A. White
Publisher : Springer Science & Business Media
Page : 397 pages
File Size : 55,6 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595020
PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.