Cytoskeleton Methods And Protocols

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Cytoskeleton Methods and Protocols

Ray H. Gavin
Cytoskeleton Methods and Protocols Book PDF

Author : Ray H. Gavin
Publisher : Springer Science & Business Media
Page : 286 pages
File Size : 55,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592590513

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Cytoskeleton Methods and Protocols by Ray H. Gavin Pdf

Over the past two decades experimental studies have solidified the int- pretation of the cytoskeleton as a highly dynamic network of microtubules, actin microfilaments, intermediate filaments, and myosin filaments. Rather than a network of disparate fibers, these polymers are often interconnected and display synergy, which is the combined action of two or more cytoskeletal polymers to achieve a specific cellular structure or function. Cross-commu- cation among cytoskeletal polymers is thought to be achieved through cytoskeletal polymer accessory proteins and molecular motors that bind two or more cytoskeletal polymers. Development of the modern concept of the cytoskeleton is a direct o- growth of advances in experimental tools and reagents that are available to cell and molecular biologists. Technological advances and refinements in cell imaging have made it possible to selectively image a single cytoskeletal po- mer and monitor its dynamics through the use of fluorescence probes in vitro and in vivo. Two decades ago, cytoskeletal research was limited to a few perturbation reagents that included colchicine and cytochalasin. Today, the perturbation arsenal has expanded to a highly selective group of reagents that includes Taxol, nocodazole, benomyl, latrunculin, jasplakinolide, and such endogenous proteins as gelsolin. These reagents enable the investigator to selectively perturb or destroy a cytoskeletal polymer while leaving other cytoskeletal polymers intact. Site-specific monoclonal antibodies that target a specific cytoskeletal polymer have proven to be highly selective affinity tools for cytoskeletal research.

Cytoskeleton

Ray H. Gavin
Cytoskeleton Book PDF

Author : Ray H. Gavin
Publisher : Unknown
Page : 0 pages
File Size : 52,9 Mb
Release : 2016
Category : Anatomy
ISBN : 1493931253

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Cytoskeleton by Ray H. Gavin Pdf

The third edition of this volume focuses on experimental models that are useful for investigating various aspects of cytoskeleton structure and function. Animal, plant, protist, and fungal models highlight twenty-four chapters that provide detailed protocols for live and fixed-cell imaging, dynamics of cytoskeleton components, cell and organelle motility, and genetics and proteomics. Written in the highly successful Methods in Molecular Biology series format, protocols in each chapter are up-to-date menus organized in a useful step-by-step format appropriate for novice and established investigators. Each chapter is equipped with a valuable notes section that provides a troubleshooting guide and helpful, and often unpublished, technical information aimed at ensuring success with implementation of the protocols. Authoritative and thorough, Cytoskeleton Methods and Protocols, Third Edition helps researchers expand their understanding of cytoskeleton structure and function.

The Plant Cytoskeleton

Patrick J. Hussey,Pengwei Wang
The Plant Cytoskeleton Book PDF

Author : Patrick J. Hussey,Pengwei Wang
Publisher : Springer Nature
Page : 368 pages
File Size : 48,9 Mb
Release : 2023-02-11
Category : Science
ISBN : 9781071628676

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The Plant Cytoskeleton by Patrick J. Hussey,Pengwei Wang Pdf

This detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.

Cytoskeleton Dynamics

Helder Maiato
Cytoskeleton Dynamics Book PDF

Author : Helder Maiato
Publisher : Unknown
Page : 390 pages
File Size : 42,6 Mb
Release : 2020
Category : Cytology
ISBN : 1071602195

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Cytoskeleton Dynamics by Helder Maiato Pdf

This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers. .

Cytoskeleton Methods and Protocols

Ray H. Gavin
Cytoskeleton Methods and Protocols Book PDF

Author : Ray H. Gavin
Publisher : Humana
Page : 0 pages
File Size : 46,7 Mb
Release : 2015-10-24
Category : Science
ISBN : 1493931237

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Cytoskeleton Methods and Protocols by Ray H. Gavin Pdf

The third edition of this volume focuses on experimental models that are useful for investigating various aspects of cytoskeleton structure and function. Animal, plant, protist, and fungal models highlight twenty-four chapters that provide detailed protocols for live and fixed-cell imaging, dynamics of cytoskeleton components, cell and organelle motility, and genetics and proteomics. Written in the highly successful Methods in Molecular Biology series format, protocols in each chapter are up-to-date menus organized in a useful step-by-step format appropriate for novice and established investigators. Each chapter is equipped with a valuable notes section that provides a troubleshooting guide and helpful, and often unpublished, technical information aimed at ensuring success with implementation of the protocols. Authoritative and thorough, Cytoskeleton Methods and Protocols, Third Edition helps researchers expand their understanding of cytoskeleton structure and function.

Mechanobiology

Ronen Zaidel-Bar
Mechanobiology Book PDF

Author : Ronen Zaidel-Bar
Publisher : Springer Nature
Page : 337 pages
File Size : 50,6 Mb
Release : 2023-01-01
Category : Sports & Recreation
ISBN : 9781071628515

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Mechanobiology by Ronen Zaidel-Bar Pdf

This detailed book collects methodologies exploring mechanobiology, the involvement of mechanical forces in cell fate specification and in controlling single and collective cell behaviors such as directed migration, morphogenesis, wound healing, and the immune response. The volume features methods to quantify the mechanical properties of cells and adhesion proteins, to expose cells to external mechanical forces, to quantitatively characterize mechano-responses at various scales, to measure forces applied by cells on the extracellular matrix, as well as chapters on force measurement inside cells, probing cell signaling and gene expression in response to force, and biophysical modeling of cell shape and protein dynamics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mechanobiology: Methods and Protocols aims to provide meaningful tools for cell and developmental biologists approaching the study of cell and tissue dynamics from a mechanobiological perspective, molecular biologists interested in the effects of force on proteins, as well as for cancer biologists and biophysicists.

Plant Cell Division

Marie-Cécile Caillaud
Plant Cell Division Book PDF

Author : Marie-Cécile Caillaud
Publisher : Humana
Page : 267 pages
File Size : 42,9 Mb
Release : 2021-10-28
Category : Science
ISBN : 1071617435

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Plant Cell Division by Marie-Cécile Caillaud Pdf

This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression.

Mitosis

David J. Sharp
Mitosis Book PDF

Author : David J. Sharp
Publisher : Humana Press
Page : 308 pages
File Size : 48,8 Mb
Release : 2014-03-15
Category : Science
ISBN : 1493903306

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Mitosis by David J. Sharp Pdf

Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the cell. The volume is divided into four parts, each focused on methods pertaining to distinct aspects of mitosis research. Part I presents approaches for visualizing and analyzing the dynamic behaviors of the spindle apparatus, the microtubule based machine that drives chromosome segregation. Part II focuses more generally on methods for studying and manipulating the microtubule cytoskeleton in cells and complex cell free extracts. Part III provides state of the art biophysical and high resolution microscopy approaches for assessing complex interactions between microtubules and microtubule-associated proteins in isolation as well as microtubule structure in cells. Part IV provides methods for studying the effects of cell shape on cell division and methods for quantifying aneuploidy (aberrant chromosome number) which frequently results from mitotic defects and has been linked to human maladies ranging from birth defects to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitosis: Methods and Protocols seeks to provide diverse methods and new techniques to address new or old questions related to the mechanisms of mitosis.

Cytoskeleton: Signalling and Cell Regulation

Kermit Carraway,Coralie Carraway
Cytoskeleton Signalling and Cell Regulation Book PDF

Author : Kermit Carraway,Coralie Carraway
Publisher : OUP Oxford
Page : 308 pages
File Size : 41,9 Mb
Release : 1999-12-16
Category : Science
ISBN : 9780191565991

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Cytoskeleton: Signalling and Cell Regulation by Kermit Carraway,Coralie Carraway Pdf

This book provides descriptions of experimental methods in research on the cytoskeleton and its relationships to signaling and cell regulation. Thus, it bridges two active and fertile areas of research. The focus is directed particularly towards methods which take advantage of recent advances in molecular biology, microscopy and immunological assays. A second emphasis is on methods for understanding dynamic changes in cells. A third emphasis is on the formation and turnover of macromolecular and supramolecular complexes, which are so important in driving cell regulation and the behaviour of cytoskeletal elements. A combination of practical advice and detailed protocols should make this book valuable for both novice and experienced workers in these burgeoning fields.

The Cytoskeleton

Kermit L. Carraway,Coralie A. Carothers Carraway
The Cytoskeleton Book PDF

Author : Kermit L. Carraway,Coralie A. Carothers Carraway
Publisher : Oxford University Press, USA
Page : 292 pages
File Size : 46,5 Mb
Release : 1992
Category : Cytology
ISBN : UOM:39015022253960

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The Cytoskeleton by Kermit L. Carraway,Coralie A. Carothers Carraway Pdf

Cytoskeletal research has undergone rapid progress in recent years and is currently an area of research activity. This book provides a wealth of methods and expert advice for investigating the cytoskeletal elements of eukaryotic cells. It focuses on the dynamic and functional aspects of the three major systems of the cytoskeleton--microfilaments, intermediate filaments and microtubules, and their interactions with their associate proteins. Detailed experimental protocols for state-of-the-art techniques are given throughout, and emphasis is placed on recent advances in areas such as video microscopy, molecular biology, immunological analysis, and microchemistry of proteins. This comprehensive volume will be useful to both experienced and novice researchers who wish to apply the technologies available for the study of these important components of eukaryotic cell structure.

Microtubule Protocols

Jun Zhou
Microtubule Protocols Book PDF

Author : Jun Zhou
Publisher : Springer Science & Business Media
Page : 307 pages
File Size : 51,8 Mb
Release : 2007-08-23
Category : Medical
ISBN : 9781588296429

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Microtubule Protocols by Jun Zhou Pdf

Microtubules are essential components of the cytoskeleton, and play critical roles in a variety of cell processes, including cell shaping, intracellular tracking, cell division, and cell migration. Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The straightforward presentation of readily reproducible protocols is a hallmark of the Methods in Molecular MedicineTM series, and is evident in this volume. Methods presented range from the purification and characterization of microtubule proteins, analysis of post-translational modifications of tubulin, and determination of microtubule structure, to the visualization of microtubule and spindle behavior, measurement of microtubule dynamics, and examination of microtubule-mediated cellular processes. Both basic scientists and clinical researchers will benefit from this collection of state-of-the-art protocols for microtubule research.

The LINC Complex

Gregg G. Gundersen,Howard J. Worman
The LINC Complex Book PDF

Author : Gregg G. Gundersen,Howard J. Worman
Publisher : Humana
Page : 338 pages
File Size : 41,5 Mb
Release : 2019-09-07
Category : Science
ISBN : 1493993674

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The LINC Complex by Gregg G. Gundersen,Howard J. Worman Pdf

This volume describes research methodologies and approaches used to study the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex and its cellular functions. Chapters detail structural and biochemical analysis of LINC complexes, mechanical aspects of the LINC complex, analysis of the LINC complex in model systems and development, and LINC complex in mammalian tissue, organs, and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The LINC Complex: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Cytoskeleton Methods and Protocols

Ray H. Gavin
Cytoskeleton Methods and Protocols Book PDF

Author : Ray H. Gavin
Publisher : Humana Press
Page : 0 pages
File Size : 52,7 Mb
Release : 2010-04-29
Category : Science
ISBN : 1607613778

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Cytoskeleton Methods and Protocols by Ray H. Gavin Pdf

In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In Cytoskeleton Methods and Protocols, Second Edition, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics. As a volume in the highly successful Methods in Molecular BiologyTM series, chapters incorporate introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes that provide unpublished technical information on troubleshooting and avoiding known pitfalls. Up-to-date and comprehensive, Cytoskeleton Methods and Protocols, Second Edition serves as an ideal guide to scientists who wish to continue this fruitful and important biological research.

Microtubule Dynamics

Anne Straube
Microtubule Dynamics Book PDF

Author : Anne Straube
Publisher : Humana Press
Page : 0 pages
File Size : 41,7 Mb
Release : 2011-07-25
Category : Science
ISBN : 1617792519

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Microtubule Dynamics by Anne Straube Pdf

Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.

Connexin Methods and Protocols

Roberto Bruzzone,Christian Giaume
Connexin Methods and Protocols Book PDF

Author : Roberto Bruzzone,Christian Giaume
Publisher : Springer Science & Business Media
Page : 495 pages
File Size : 47,8 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590438

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Connexin Methods and Protocols by Roberto Bruzzone,Christian Giaume Pdf

Direct cell–cell communication is a common property of multicellular organisms that is achieved through membrane channels which are organized in gap junctions. The protein subunits of these intercellular channels, the connexins, form a multigene family that has been investigated in great detail in recent years. It has now become clear that, in different tissues, connexins speak several languages that control specific cellular functions. This progress has been made possible by the availability of new molecular tools and the improvement of basic techniques for the study of membrane channels, as well as by the use of genetic approaches to study protein function in vivo. More important, connexins have gained visibility because mutations in some connexin genes have been found to be linked to human genetic disorders. Connexin Methods and Protocols presents in detail a collection of te- niques currently used to study the cellular and molecular biology of connexins and their physiological properties. The field of gap junctions and connexin research has always been characterized by a multidisciplinary approach c- bining morphology, biochemistry, biophysics, and cellular and molecular biology. This book provides a series of cutting-edge protocols and includes a large spectrum of practical methods that are available to investigate the fu- tion of connexin channels. Connexin Methods and Protocols is divided into three main parts.