Detection Of Non Amplified Genomic Dna

Author : Giuseppe Spoto,Roberto Corradini
Publisher : Springer Science & Business Media
Page : 320 pages
File Size : 47,6 Mb
Release : 2012-07-06
Category : Science
ISBN : 9789400712263

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Detection of Non-Amplified Genomic DNA by Giuseppe Spoto,Roberto Corradini Pdf

This book offers an overview of state-of-the-art in non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in this field.

Author : Vadim V. Demidov,Natalia E. Broude
Publisher : Taylor & Francis
Page : 342 pages
File Size : 49,6 Mb
Release : 2004
Category : Science
ISBN : 0954523296

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DNA Amplification by Vadim V. Demidov,Natalia E. Broude Pdf

Whereas most books on DNA amplification focus on PCR-based technologies, this volume presents a wider range of methods to amplify DNA with an emphasis on their diverse applications. The book covers both well-established and newly-developed protocols including ligation-based thermocycling approaches, real-time PCR and other new PCR developments, plus several powerful non-PCR isothermal DNA amplification techniques, for example: real-time strand displacement amplification (SDA), rolling-circle amplification (RCA) and multiple-displacement amplification (MDA). An entire section is devoted to a group of enzymes, both natural and engineered, which are employed for DNA amplification and related purposes. In addition, the use of DNA amplification in the detection of non-DNA analytes is presented.

Author : Simon Hughes,Roger S. Lasken
Publisher : Scion Publishing Ltd
Page : 217 pages
File Size : 45,5 Mb
Release : 2005-09-01
Category : Science
ISBN : 9781907904448

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Whole Genome Amplification by Simon Hughes,Roger S. Lasken Pdf

Whole genome amplification generates microgram quantities of genomic DNA starting from a sample of as little as a few femtograms and so is a vital technique when sample material is limited, as well as for high-throughput assays. Whole Genome Amplification: Methods Express is a comprehensive up-to-date laboratory manual for this key technique. It provides detailed step-by-step protocols as well as hints and tips for success and troubleshooting, taking readers through all aspects of whole genome amplification. This book is an essential practical guide for any researcher currently using PCR for genomic amplification or who wishes to do so in future.

Author : Bimal D. M. Theophilus,Ralph Rapley
Publisher : Springer Science & Business Media
Page : 214 pages
File Size : 43,9 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592592739

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PCR Mutation Detection Protocols by Bimal D. M. Theophilus,Ralph Rapley Pdf

1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

Author : Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White
Publisher : Academic Press
Page : 501 pages
File Size : 45,9 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780080886718

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PCR Protocols by Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White Pdf

The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications

Author : Sergey Yurish
Publisher : Lulu.com
Page : 560 pages
File Size : 42,8 Mb
Release : 2014-07-14
Category : Technology & Engineering
ISBN : 9788461641543

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Sensors and Biosensors, MEMS Technologies and its Applications by Sergey Yurish Pdf

Sensors and Biosensors, MEMS Technologies and its Applications (Book Series: Advances in Sensors: Reviews, Vol. 2) - 18 chapters with sensor related state-of-the-art reviews and descriptions of the latest achievements written by experts from academia and industry from 12 countries: China, India, Iran, Malaysia, Poland, Singapore, Spain, Taiwan, Thailand, UK, Ukraine and USA. This volume is divided into three main parts: physical sensors, biosensors, nanoparticles, MEMS technologies and applications. With this unique combination of information in each volume, the Advances in Sensors: Reviews Book Series will be of value for scientists and engineers in industry and at universities, to sensors developers, distributors, and users. Like the 1st volume of this Book Series, the 2nd volume also has been organized by topics of high interest.

Author : Salah E. O. Mahgoub,Leo M.L. Nollet
Publisher : CRC Press
Page : 329 pages
File Size : 46,9 Mb
Release : 2019-01-15
Category : Health & Fitness
ISBN : 9780429685323

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Testing and Analysis of GMO-containing Foods and Feed by Salah E. O. Mahgoub,Leo M.L. Nollet Pdf

An increasing number of genetically modified organisms (GMOs) continues to be produced every day. In response to the concerns raised by the development of GMOs and their incorporation in foods and feed, guidelines and regulations to govern and control the use of GMOs and their products have been enacted. These regulations necessitated the design of methods to detect and analyse the presence of GMOs or their products in agriculture produce, food and feed production chains. Design of techniques and instruments that would detect, identify, and quantify GM ingredients in food and feed will help inspection authorities to relay reliable information to consumers who might be concerned about the presence of GM ingredients. Information generated by detection of GMOs in food and feed would be helpful for setting regulations that govern the use of GM components as well as for labeling purposes. Qualitative detection methods of GM-DNA sequences in foods and feeds have evolved fast during the past few years. There is continuous need for the development of more advanced multi-detection systems and for periodic updates of the databases related to these systems. Testing and Analysis of GMO-containing Foods and Feed presents updates and comprehensive views on the various methods and techniques in use today for the detection, identification and quantification of GMOs in foods and feed. The eleven book chapters cover recent developments on sample preparation techniques, immunoassays methods and the PCR technique used in GMO analysis, the use of biosensors in relation to GMO analysis, the application of nucleic acid microarrays for the detection of GMOs, validation and standardization methods for GMO testing, in addition to the type of reference material and reference methods used in GMO testing and analysis. Some of the ISO standards designed for identifying and detecting the presence of GM material in foods are also presented in the book.

Author : Godfrey M. Hewitt,Andrew W.B. Johnston,J.Peter W. Young
Publisher : Springer Science & Business Media
Page : 404 pages
File Size : 49,9 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783642839627

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Molecular Techniques in Taxonomy by Godfrey M. Hewitt,Andrew W.B. Johnston,J.Peter W. Young Pdf

Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing.

Author : Claudia Steinem,Andreas Janshoff
Publisher : Springer Science & Business Media
Page : 485 pages
File Size : 46,9 Mb
Release : 2007-04-30
Category : Science
ISBN : 9783540365686

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Piezoelectric Sensors by Claudia Steinem,Andreas Janshoff Pdf

This volume includes a comprehensive theoretical treatment and current state-of-the art applications of the quartz crystal microbalance (QCM). It discusses interface circuits and the study of viscoelasticity and micromechanics as well as surface roughness with the QCM. Coverage also details the broad field of analytical applications of piezoelectric sensors.

Author : Kary B. Mullis,Francois Ferre,Richard A. Gibbs
Publisher : Springer Science & Business Media
Page : 464 pages
File Size : 54,5 Mb
Release : 2012-02-02
Category : Medical
ISBN : 9781461202578

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The Polymerase Chain Reaction by Kary B. Mullis,Francois Ferre,Richard A. Gibbs Pdf

James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...

Author : Kirstin J. Edwards,Julie M. J. Logan,Nick A. Saunders
Publisher : Taylor & Francis
Page : 362 pages
File Size : 40,7 Mb
Release : 2004
Category : Polymerase chain reaction
ISBN : 9781134184002

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Real-Time PCR by Kirstin J. Edwards,Julie M. J. Logan,Nick A. Saunders Pdf

Author : Zerong Wang,Uta Wille,Eusebio Juaristi
Publisher : John Wiley & Sons
Page : 4542 pages
File Size : 55,7 Mb
Release : 2017-04-17
Category : Science
ISBN : 9781118470459

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Encyclopedia of Physical Organic Chemistry, 6 Volume Set by Zerong Wang,Uta Wille,Eusebio Juaristi Pdf

Winner of 2018 PROSE Award for MULTIVOLUME REFERENCE/SCIENCE This encyclopedia offers a comprehensive and easy reference to physical organic chemistry (POC) methodology and techniques. It puts POC, a classical and fundamental discipline of chemistry, into the context of modern and dynamic fields like biochemical processes, materials science, and molecular electronics. Covers basic terms and theories into organic reactions and mechanisms, molecular designs and syntheses, tools and experimental techniques, and applications and future directions Includes coverage of green chemistry and polymerization reactions Reviews different strategies for molecular design and synthesis of functional molecules Discusses computational methods, software packages, and more than 34 kinds of spectroscopies and techniques for studying structures and mechanisms Explores applications in areas from biology to materials science The Encyclopedia of Physical Organic Chemistry has won the 2018 PROSE Award for MULTIVOLUME REFERENCE/SCIENCE. The PROSE Awards recognize the best books, journals and digital content produced by professional and scholarly publishers. Submissions are reviewed by a panel of 18 judges that includes editors, academics, publishers and research librarians who evaluate each work for its contribution to professional and scholarly publishing. You can find out more at: proseawards.com Also available as an online edition for your library, for more details visit Wiley Online Library

Author : Francois Ferre
Publisher : Springer Science & Business Media
Page : 379 pages
File Size : 53,9 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461241645

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Gene Quantification by Francois Ferre Pdf

Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.

Author : Dimitrios P. Nikolelis,Theodoros Varzakas,Arzum Erdem,Georgia-Paraskevi Nikoleli
Publisher : CRC Press
Page : 830 pages
File Size : 46,9 Mb
Release : 2013-10-21
Category : Science
ISBN : 9781466576339

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Portable Biosensing of Food Toxicants and Environmental Pollutants by Dimitrios P. Nikolelis,Theodoros Varzakas,Arzum Erdem,Georgia-Paraskevi Nikoleli Pdf

Biosensors are poised to make a large impact in environmental, food, and biomedical applications, as they clearly offer advantages over standard analytical methods, including minimal sample preparation and handling, real-time detection, rapid detection of analytes, and the ability to be used by non-skilled personnel. Covering numerous applications of biosensors used in food and the environment, Portable Biosensing of Food Toxicants and Environmental Pollutants presents basic knowledge on biosensor technology at a postgraduate level and explores the latest advances in chemical sensor technology for researchers. By providing useful, state-of-the-art information on recent developments in biosensing devices, the book offers both newcomers and experts a roadmap to this technology. In the book, distinguished researchers from around the world show how portable and handheld nanosensors, such as dynamic DNA and protein arrays, enable rapid and accurate detection of environmental pollutants and pathogens. The book first introduces the basic principles of biosensing for newcomers to the technology. It then explains how the integration of a "receptor" can provide analytically useful information. It also describes trends in biosensing and examines how a small-sized device can have portability for the in situ determination of toxicants. The book concludes with several examples illustrating how to determine toxicants in food and environmental samples.

Author : Anonim
Publisher : ScholarlyEditions
Page : 1260 pages
File Size : 50,8 Mb
Release : 2012-01-09
Category : Science
ISBN : 9781464964657

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Issues in Biotechnology and Medical Technology Research and Application: 2011 Edition by Anonim Pdf

Issues in Biotechnology and Medical Technology Research and Application: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Biotechnology and Medical Technology Research and Application. The editors have built Issues in Biotechnology and Medical Technology Research and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Biotechnology and Medical Technology Research and Application in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Biotechnology and Medical Technology Research and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.