Dna Protein Interactions In Transcription

Author : A. Travers
Publisher : Springer Science & Business Media
Page : 189 pages
File Size : 51,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401114806

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DNA-Protein Interactions by A. Travers Pdf

Our understanding of the mechanisms regulating gene expression, which determine the patterns of growth and development in all living organisms, ultimately involves the elucidation of the detailed and dy namic interactions of proteins with nucleic acids -both DNA and RNA. Until recently the commonly presented view of the DNA double helix as visualized on the covers of many textbooks and journals - was as a monotonous static straight rod incapable in its own right of directing the processes necessary for the conservation and selective reading of genetic information. This view, although perhaps extreme, was reinforced by the necessary linearity of genetic maps. The reality is that the biological functions of both DNA and RNA are dependent on complex, and sometimes transient, three-dimensional nucleoprotein structures in which genetically distant elements are brought into close spatial proximity. It is in such structures that the enzymatic manipulation of DNA in the essential biological processes as DNA replication, transcription and recombination are effected - the complexes are the mediators of the 'DNA transactions' of Hatch Echols.

Author : Jay D. Gralla
Publisher : Alan R. Liss
Page : 360 pages
File Size : 54,7 Mb
Release : 1989
Category : Science
ISBN : UCAL:B4456665

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DNA-protein Interactions in Transcription by Jay D. Gralla Pdf

Author : Tom Moss
Publisher : Springer Science & Business Media
Page : 633 pages
File Size : 48,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592592081

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DNA'Protein Interactions by Tom Moss Pdf

Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.

Author : Mark C. Williams,L. James Maher, III
Publisher : Springer Science & Business Media
Page : 354 pages
File Size : 53,7 Mb
Release : 2010-10-05
Category : Science
ISBN : 9780387928081

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Biophysics of DNA-Protein Interactions by Mark C. Williams,L. James Maher, III Pdf

Depite the rapid expansion of the field of biophysics, there are very few books that comprehensively treat specific topics in this area. Recently, the field of single molecule biophysics has developed very quickly, and a few books specifically treating single molecule methods are beginning to appear. However, the promise of single molecule biophysics is to contribute to the understanding of specific fields of biology using new methods. This book would focus on the specific topic of the biophysics of DNA-protein interactions, and would include the use of new approaches, including both bulk methods as well as single molecule methods. This would make the book attractive to anyone working in the general area of DNA-protein interactions, which is of course a much wider market than just single molecule biophysicists or even biophysicists. The subject of the book will be the biophysics of DNA-protein interactions, and will include new methods and results that describe the physical mechanism by which proteins interact with DNA. For example, there has been much recent work on the mechanism by which proteins search for specific binding sites on DNA. A few chapters will be devoted to experiments and theory that shed light on this important problem. We will also cover proteins that alter DNA properties to facilitate interactions important for transcription or replication. Another section of the book will cover the biophysical mechanism by which motor proteins interact with DNA. Finally, we will cover larger protein-DNA complexes, such as replication forks, recombination complexes, DNA repair interactions, and their chromatin context.

Author : Andrew Arthur Travers,Malcolm Buckle
Publisher : Oxford University Press, USA
Page : 416 pages
File Size : 41,7 Mb
Release : 2000
Category : Medical
ISBN : 0199636915

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DNA-protein Interactions by Andrew Arthur Travers,Malcolm Buckle Pdf

DNA-Protein Interactions is a novel compilation of methods for studying the interactions of proteins with DNA. It is a rapidly advancing research area in which multidisciplinary approaches are especially valuable for solving problems and obtaining a detailed understanding of the molecular regulatory interactions involved. This book covers all the major tools that are required for the study of the large macromolecular enzymatic machines that manipulate DNA, with particular emphasis on biophysical techniques applied to the analysis of transcription and its relation to chromatin structure. Knowledge of basic techniques is assumed, although advances in fundamental fields are covered.

Author : Andrew Arthur Travers
Publisher : Unknown
Page : 180 pages
File Size : 51,7 Mb
Release : 1993
Category : DNA-protein interactions
ISBN : OCLC:1175275839

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DNA-protein Interactions by Andrew Arthur Travers Pdf

Author : Christopher W.J. Smith
Publisher : Oxford University Press, UK
Page : 370 pages
File Size : 47,7 Mb
Release : 1998-07-09
Category : Electronic
ISBN : 9780191591624

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RNA-Protein Interactions : A Practical Approach by Christopher W.J. Smith Pdf

RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Recent research into the role of RNA in cells has elucidated many more vital interactions with proteins. This book provides an up-to-date and comprehensive guide to a wide range of laboratory procedures to investigate the interactions between RNA and proteins. - ;RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field. -

Author : Arnold Revzin
Publisher : CRC Press
Page : 278 pages
File Size : 46,7 Mb
Release : 1990-08-27
Category : Medical
ISBN : 084936177X

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The Biology of Nonspecific DNA Protein Interactions by Arnold Revzin Pdf

This important publication addresses the interactions of proteins with nonspecific binding sites on DNA as they play critical roles in fundamental cellular processes such as transcription, DNA replication, and recombination. The book presents current reviews of the biochemistry of representative nonspecific DNA-protein systems, and of their physiological functions. It includes chapters on the techniques used to characterize the complexes, on their thermodynamic properties, and on the role of nonspecific binding as gene regulatory proteins search for specific target sites on the chromosome. Systems considered include the effects of nonspecific binding in regulation of the lactose operon of Escherichia coli, the T4 bacteriophage gene 32 protein, the E. coli single strand binding (SSB) protein and recA protein, eukaryotic SSB's and histone-DNA complexes. The book presents those proteins displaying multiple modes of DNA binding as participants in more than one cellular process. This monograph combines rigorous descriptions of new findings for these important systems with provocative interpretations of the biological significance of the results. It is of great value to researchers ranging from graduate students to senior scientists in the areas of biochemistry, microbiology and molecular/cell biology.

Author : Phoebe A. Rice,Carl C. Correll
Publisher : Royal Society of Chemistry
Page : 417 pages
File Size : 41,9 Mb
Release : 2008-05-22
Category : Science
ISBN : 9780854042722

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Protein-Nucleic Acid Interactions by Phoebe A. Rice,Carl C. Correll Pdf

This book provides both in-depth background and up-to-date information in this area. The chapters are organized by general themes and principles, written by experts who illustrate topics with current findings. Topics covered include: - the role of ions and hydration in protein-nucleic acid interactions - transcription factors and combinatorial specificity - indirect readout of DNA sequence - single-stranded nucleic acid binding proteins - nucleic acid junctions and proteins, - RNA protein recognition - recognition of DNA damage. It will be a key reference for both advanced students and established scientists wishing to broaden their horizons.

Author : Jay D. Gralla
Publisher : John Wiley & Sons
Page : 334 pages
File Size : 40,6 Mb
Release : 1988-12-01
Category : Electronic
ISBN : 0471506958

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DNA-Protein Interactions in Transcription by Jay D. Gralla Pdf

Author : Harald Seitz
Publisher : Springer Science & Business Media
Page : 212 pages
File Size : 46,7 Mb
Release : 2007-01-16
Category : Medical
ISBN : 9783540481478

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Analytics of Protein-DNA Interactions by Harald Seitz Pdf

With contributions by numerous experts

Author : Marcos Simoes-Costa
Publisher : Springer Nature
Page : 288 pages
File Size : 47,8 Mb
Release : 2022-11-25
Category : Science
ISBN : 9781071628478

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DNA-Protein Interactions by Marcos Simoes-Costa Pdf

This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, DNA-Protein Interactions: Methods and Protocols aims to present genome-wide techniques that will complement the biochemistry-based protocols to aid researchers in their studies.

Author : Gary Stormo
Publisher : Unknown
Page : 198 pages
File Size : 41,8 Mb
Release : 2013
Category : Science
ISBN : 193611349X

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Introduction to Protein-DNA Interactions by Gary Stormo Pdf

One of the foundations of molecular biology is how the interactions of proteins with DNA control many aspects of gene expression. Since the mid-20th century discoveries of the lac repressor and operator and the competition between the cI and cro proteins for the same segment of DNA, we have learned an enormous amount about the interactions of proteins with DNA and their control of fundamental processes in the cell. Introduction to Protein-DNA Interactions: Structure, Thermodynamics, and Bioinformatics describes what we know about protein-DNA interactions from the complementary perspectives of molecular and structural biology and bioinformatics and how each perspective informs the others. A particular emphasis is on how insights from experimental work can be translated into specific computational approaches to create unified view of the field and a fuller understanding of protein-DNA interactions.

Author : Kenneth Berendzen,Joachim Kilian ,Dierk Wanke
Publisher : Bentham Science Publishers
Page : 225 pages
File Size : 44,6 Mb
Release : 2013-10-29
Category : Science
ISBN : 9781608054923

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The Analysis of Regulatory DNA: Current Developments, Knowledge and Applications Uncovering Gene Regulation by Kenneth Berendzen,Joachim Kilian ,Dierk Wanke Pdf

A major goal of integrative research is understanding regulatory networks to such an extent as to allow researchers to model developmental and stress responses. Regulatory networks of living systems include complex and vast interactions between proteins, metabolites, RNA, various signaling molecules and DNA. One aspect of systems biology is understanding the dynamics of protein-DNA interactions affecting gene expression that are caused by transcription factors (TFs) and chromatin remodeling factors. This e-book provides a resource for summarizing current knowledge eukaryotic transcription and explores cis-elements and methods for their analysis, prediction and discovery. The book also presents an overview of exploring gene regulatory networks, chromatin, and miRNAs. Information about state-of-the-art techniques for the determination of TF - cis-element interactions in vivo and in silico give cutting edge insights on how genomic-scale research is being approached. The Analysis of Regulatory DNA provides readers with both the necessary background knowledge and provocative, up-to-date insights aimed at sparking new and vibrant experimental designs for understanding and predicting cis-elements in the eukaryotic genome.

Author : David Scott Gilmour
Publisher : Unknown
Page : 400 pages
File Size : 50,8 Mb
Release : 1984
Category : DNA.
ISBN : CORNELL:31924001570401

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Detection of DNA-protein Interactions by Protein-DNA Cross-linking by David Scott Gilmour Pdf