Rna Protein Interactions A Practical Approach

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RNA-Protein Interactions : A Practical Approach

Christopher W.J. Smith
RNA Protein Interactions A Practical Approach Book PDF

Author : Christopher W.J. Smith
Publisher : Oxford University Press, UK
Page : 370 pages
File Size : 47,6 Mb
Release : 1998-07-09
Category : Electronic
ISBN : 9780191591624

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RNA-Protein Interactions : A Practical Approach by Christopher W.J. Smith Pdf

RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Recent research into the role of RNA in cells has elucidated many more vital interactions with proteins. This book provides an up-to-date and comprehensive guide to a wide range of laboratory procedures to investigate the interactions between RNA and proteins. - ;RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field. -

DNA-protein Interactions

Andrew Arthur Travers,Malcolm Buckle
DNA protein Interactions Book PDF

Author : Andrew Arthur Travers,Malcolm Buckle
Publisher : Oxford University Press, USA
Page : 416 pages
File Size : 40,9 Mb
Release : 2000
Category : Medical
ISBN : 0199636915

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DNA-protein Interactions by Andrew Arthur Travers,Malcolm Buckle Pdf

DNA-Protein Interactions is a novel compilation of methods for studying the interactions of proteins with DNA. It is a rapidly advancing research area in which multidisciplinary approaches are especially valuable for solving problems and obtaining a detailed understanding of the molecular regulatory interactions involved. This book covers all the major tools that are required for the study of the large macromolecular enzymatic machines that manipulate DNA, with particular emphasis on biophysical techniques applied to the analysis of transcription and its relation to chromatin structure. Knowledge of basic techniques is assumed, although advances in fundamental fields are covered.

RNA-Protein Complexes and Interactions

Ren-Jang Lin
RNA Protein Complexes and Interactions Book PDF

Author : Ren-Jang Lin
Publisher : Springer Nature
Page : 349 pages
File Size : 54,8 Mb
Release : 2023-05-11
Category : Science
ISBN : 9781071631911

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RNA-Protein Complexes and Interactions by Ren-Jang Lin Pdf

This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA-Protein Complexes and Interactions: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.

RNA'Protein Interaction Protocols

Susan R. Haynes
RNA Protein Interaction Protocols Book PDF

Author : Susan R. Haynes
Publisher : Springer Science & Business Media
Page : 485 pages
File Size : 51,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596768

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RNA'Protein Interaction Protocols by Susan R. Haynes Pdf

The molecular characterization of RNA and its interactions with proteins is an important and exciting area of current research. Organisms utilize a variety of RNA–protein interactions to regulate the expression of their genes. This is particularly true for eukaryotes, since newly synthesized messenger RNA must be extensively modified and transported to the cytoplasm before it can be used for protein synthesis. The realization that posttranscriptional processes are critical components of gene regulation has sparked an explosion of interest in both stable ribonucleoprotein (RNP) complexes and transient RNA–protein interactions. RNA is conformationally flexible and can adopt complex structures that provide diverse surfaces for interactions with proteins. The fact that short RNA molecules (aptamers; see Chapter 16) can be selected to bind many different types of molecules is evidence of the structural variability of RNA. RNA molecules are rarely entirely single- or double-stranded, but usually contain multiple short duplexes interrupted by single-stranded loops and bulges; in some RNAs, such as tRNAs, the short duplexes stack on each other. Further variability is generated by the presence of non-Watson-Crick base pairs, modified nucleotides, and more complex structures, such as pseudoknots and triple-strand interactions.

RNA-protein Interactions

Kiyoshi Nagai,Iain W. Mattaj
RNA protein Interactions Book PDF

Author : Kiyoshi Nagai,Iain W. Mattaj
Publisher : Oxford University Press, USA
Page : 302 pages
File Size : 43,5 Mb
Release : 1994
Category : Medical
ISBN : UOM:39015031725248

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RNA-protein Interactions by Kiyoshi Nagai,Iain W. Mattaj Pdf

The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.

Biophysics of RNA-Protein Interactions

Chirlmin Joo,David Rueda
Biophysics of RNA Protein Interactions Book PDF

Author : Chirlmin Joo,David Rueda
Publisher : Springer Nature
Page : 249 pages
File Size : 53,6 Mb
Release : 2019-09-19
Category : Science
ISBN : 9781493997268

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Biophysics of RNA-Protein Interactions by Chirlmin Joo,David Rueda Pdf

RNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling.

RNA-Ligand Interactions, Part B: Molecular Biology Methods

Anonim
RNA Ligand Interactions Part B Molecular Biology Methods Book PDF

Author : Anonim
Publisher : Elsevier
Page : 579 pages
File Size : 52,6 Mb
Release : 2000-06-26
Category : Science
ISBN : 9780080496757

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RNA-Ligand Interactions, Part B: Molecular Biology Methods by Anonim Pdf

RNA-Ligand Interactions, Part B focuses on molecular biology methods. Major topics covered include: solution probe methods, tethered-probe methodologies, in vitro affinity selection methodologies, genetic methodologies for detecting RNA-protein interactions, protein engineering methodologies useful for RNA-protein interaction studies, and cell biology methods.RNA-Ligand Interactions, Part A, its companion, VOLUME 317 focuses on structural biology methods.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

RNA Methodologies

Robert E. Farrell, Jr.
RNA Methodologies Book PDF

Author : Robert E. Farrell, Jr.
Publisher : Elsevier
Page : 688 pages
File Size : 44,7 Mb
Release : 2010-07-22
Category : Science
ISBN : 9780080454764

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RNA Methodologies by Robert E. Farrell, Jr. Pdf

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Ribosomes and Protein Synthesis

Gary Spedding
Ribosomes and Protein Synthesis Book PDF

Author : Gary Spedding
Publisher : Oxford University Press, USA
Page : 352 pages
File Size : 54,9 Mb
Release : 1990
Category : Science
ISBN : UOM:39015059001613

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Ribosomes and Protein Synthesis by Gary Spedding Pdf

A practical and self-contained introduction to methods of researching the structure and function of the ribosome in light of the increasing recognition of the potential capability of RNA molecules to act as molecular catalysts. Also describes protein synthesis and cell-free synthesizing systems. Annotation copyrighted by Book News, Inc., Portland, OR

RNA Modification

Anonim
RNA Modification Book PDF

Author : Anonim
Publisher : Elsevier
Page : 480 pages
File Size : 51,9 Mb
Release : 2007-09-13
Category : Science
ISBN : 0080550916

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RNA Modification by Anonim Pdf

The presence of modified nucleotides in cellular RNAs has been known for decades and over 100 distinct RNA modifications have been characterized to date. While the exact role of many of these modifications is still unclear, many are highly conserved across evolution and most contribute to the overall fitness of the organism. In recent years, new methods and bioinformatics approaches have been developed for the dissection of modification pathways and functions. These methods intersect a number of related fields, ranging from RNA processing to comparative genomics and systems biology. In addition, many of the techniques described in this volume have broad applicability, particularly in regards to the isolation, characterization, and reconstitution of ribonucleoprotein complexes, expanding the experimental repertoire available to all RNA researchers.

Protein-Nucleic Acid Interactions

Phoebe A. Rice,Carl C. Correll
Protein Nucleic Acid Interactions Book PDF

Author : Phoebe A. Rice,Carl C. Correll
Publisher : Royal Society of Chemistry
Page : 417 pages
File Size : 41,9 Mb
Release : 2008-05-22
Category : Science
ISBN : 9780854042722

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Protein-Nucleic Acid Interactions by Phoebe A. Rice,Carl C. Correll Pdf

This book provides both in-depth background and up-to-date information in this area. The chapters are organized by general themes and principles, written by experts who illustrate topics with current findings. Topics covered include: - the role of ions and hydration in protein-nucleic acid interactions - transcription factors and combinatorial specificity - indirect readout of DNA sequence - single-stranded nucleic acid binding proteins - nucleic acid junctions and proteins, - RNA protein recognition - recognition of DNA damage. It will be a key reference for both advanced students and established scientists wishing to broaden their horizons.

Protein-Protein Interactions

Weibo Cai,Hao Hong
Protein Protein Interactions Book PDF

Author : Weibo Cai,Hao Hong
Publisher : BoD – Books on Demand
Page : 488 pages
File Size : 54,5 Mb
Release : 2012-03-30
Category : Science
ISBN : 9789535103974

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Protein-Protein Interactions by Weibo Cai,Hao Hong Pdf

Proteins are indispensable players in virtually all biological events. The functions of proteins are coordinated through intricate regulatory networks of transient protein-protein interactions (PPIs). To predict and/or study PPIs, a wide variety of techniques have been developed over the last several decades. Many in vitro and in vivo assays have been implemented to explore the mechanism of these ubiquitous interactions. However, despite significant advances in these experimental approaches, many limitations exist such as false-positives/false-negatives, difficulty in obtaining crystal structures of proteins, challenges in the detection of transient PPI, among others. To overcome these limitations, many computational approaches have been developed which are becoming increasingly widely used to facilitate the investigation of PPIs. This book has gathered an ensemble of experts in the field, in 22 chapters, which have been broadly categorized into Computational Approaches, Experimental Approaches, and Others.

Molecular Plant Biology

P. M. Gilmartin,Dr. Chris Bowler
Molecular Plant Biology Book PDF

Author : P. M. Gilmartin,Dr. Chris Bowler
Publisher : Oxford University Press, USA
Page : 376 pages
File Size : 40,9 Mb
Release : 2002
Category : Plant molecular biology
ISBN : 0199638187

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Molecular Plant Biology by P. M. Gilmartin,Dr. Chris Bowler Pdf

Molecular Plant Biology is an all-new replacement for the original Practical Approach book Plant Molecular Biology that was first published in 1988. The rapid advances made in plant sciences during the past decade are reflected by the need to produce a two-volume book to cover all the relevant methodologies. The new book incorporates many of the fundamental procedures outlined in the original book, but these are fully updated to reflect advances technology and the development of new methodologies. It also incorporates many approaches that were not available in the earlier volume. (Midwest).

Analyzing Microarray Gene Expression Data

Geoffrey J. McLachlan,Kim-Anh Do,Christophe Ambroise
Analyzing Microarray Gene Expression Data Book PDF

Author : Geoffrey J. McLachlan,Kim-Anh Do,Christophe Ambroise
Publisher : John Wiley & Sons
Page : 366 pages
File Size : 54,8 Mb
Release : 2005-02-18
Category : Mathematics
ISBN : 9780471726128

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Analyzing Microarray Gene Expression Data by Geoffrey J. McLachlan,Kim-Anh Do,Christophe Ambroise Pdf

A multi-discipline, hands-on guide to microarray analysis of biological processes Analyzing Microarray Gene Expression Data provides a comprehensive review of available methodologies for the analysis of data derived from the latest DNA microarray technologies. Designed for biostatisticians entering the field of microarray analysis as well as biologists seeking to more effectively analyze their own experimental data, the text features a unique interdisciplinary approach and a combined academic and practical perspective that offers readers the most complete and applied coverage of the subject matter to date. Following a basic overview of the biological and technical principles behind microarray experimentation, the text provides a look at some of the most effective tools and procedures for achieving optimum reliability and reproducibility of research results, including: An in-depth account of the detection of genes that are differentially expressed across a number of classes of tissues Extensive coverage of both cluster analysis and discriminant analysis of microarray data and the growing applications of both methodologies A model-based approach to cluster analysis, with emphasis on the use of the EMMIX-GENE procedure for the clustering of tissue samples The latest data cleaning and normalization procedures The uses of microarray expression data for providing important prognostic information on the outcome of disease

Bioinformatics

Andreas D. Baxevanis,B. F. Francis Ouellette
Bioinformatics Book PDF

Author : Andreas D. Baxevanis,B. F. Francis Ouellette
Publisher : John Wiley & Sons
Page : 504 pages
File Size : 40,9 Mb
Release : 2004-03-24
Category : Computers
ISBN : 9780471461012

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Bioinformatics by Andreas D. Baxevanis,B. F. Francis Ouellette Pdf

"In this book, Andy Baxevanis and Francis Ouellette . . . haveundertaken the difficult task of organizing the knowledge in thisfield in a logical progression and presenting it in a digestibleform. And they have done an excellent job. This fine text will makea major impact on biological research and, in turn, on progress inbiomedicine. We are all in their debt." —Eric Lander from the Foreword Reviews from the First Edition "...provides a broad overview of the basic tools for sequenceanalysis ... For biologists approaching this subject for the firsttime, it will be a very useful handbook to keep on the shelf afterthe first reading, close to the computer." —Nature Structural Biology "...should be in the personal library of any biologist who usesthe Internet for the analysis of DNA and protein sequencedata." —Science "...a wonderful primer designed to navigate the novice throughthe intricacies of in scripto analysis ... The accomplished genesearcher will also find this book a useful addition to theirlibrary ... an excellent reference to the principles ofbioinformatics." —Trends in Biochemical Sciences This new edition of the highly successful Bioinformatics:A Practical Guide to the Analysis of Genes and Proteinsprovides a sound foundation of basic concepts, with practicaldiscussions and comparisons of both computational tools anddatabases relevant to biological research. Equipping biologists with the modern tools necessary to solvepractical problems in sequence data analysis, the Second Editioncovers the broad spectrum of topics in bioinformatics, ranging fromInternet concepts to predictive algorithms used on sequence,structure, and expression data. With chapters written by experts inthe field, this up-to-date reference thoroughly covers vitalconcepts and is appropriate for both the novice and the experiencedpractitioner. Written in clear, simple language, the book isaccessible to users without an advanced mathematical or computerscience background. This new edition includes: All new end-of-chapter Web resources, bibliographies, andproblem sets Accompanying Web site containing the answers to the problems,as well as links to relevant Web resources New coverage of comparative genomics, large-scale genomeanalysis, sequence assembly, and expressed sequence tags A glossary of commonly used terms in bioinformatics andgenomics Bioinformatics: A Practical Guide to the Analysis of Genesand Proteins, Second Edition is essential reading forresearchers, instructors, and students of all levels in molecularbiology and bioinformatics, as well as for investigators involvedin genomics, positional cloning, clinical research, andcomputational biology.