Protein Nucleic Acid Interactions

Author : Phoebe A. Rice,Carl C. Correll
Publisher : Royal Society of Chemistry
Page : 417 pages
File Size : 46,9 Mb
Release : 2008-05-22
Category : Science
ISBN : 9780854042722

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Protein-Nucleic Acid Interactions by Phoebe A. Rice,Carl C. Correll Pdf

This book provides both in-depth background and up-to-date information in this area. The chapters are organized by general themes and principles, written by experts who illustrate topics with current findings. Topics covered include: - the role of ions and hydration in protein-nucleic acid interactions - transcription factors and combinatorial specificity - indirect readout of DNA sequence - single-stranded nucleic acid binding proteins - nucleic acid junctions and proteins, - RNA protein recognition - recognition of DNA damage. It will be a key reference for both advanced students and established scientists wishing to broaden their horizons.

Author : Wolfram Saenger,Udo Heinemann
Publisher : Unknown
Page : 230 pages
File Size : 46,8 Mb
Release : 1989
Category : Science
ISBN : STANFORD:36105030941228

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Protein-nucleic Acid Interaction by Wolfram Saenger,Udo Heinemann Pdf

This volume contains a series of essays which describe a range of problems in the field of nucleic-acid interactions, investigated by a variety of techniques. An introductory chapter on DNA-protein interactions in the regulation of gene expression is followed by papers on selected model systems.

Author : Thomas A. Steitz
Publisher : CUP Archive
Page : 108 pages
File Size : 51,8 Mb
Release : 1993-09-16
Category : Science
ISBN : 052141489X

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Structural Studies of Protein-Nucleic Acid Interaction by Thomas A. Steitz Pdf

In this 1993 text, Nobel Prize winner Professor Steitz reviews the wide-ranging research in structural studies of DNA-binding proteins and their complexes with DNA. The author clearly and concisely describes the uses of techniques in molecular genetics, DNA synthesis, protein crystallography and nuclear magnetic response.

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 50,7 Mb
Release : 1999
Category : Electronic
ISBN : OCLC:64147745

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Protein-nucleic acid interactions by Anonim Pdf

Author : Tom Moss
Publisher : Springer Science & Business Media
Page : 633 pages
File Size : 49,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592592081

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DNA'Protein Interactions by Tom Moss Pdf

Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.

Author : W. Guschlbauer
Publisher : Springer Science & Business Media
Page : 289 pages
File Size : 40,9 Mb
Release : 2013-06-29
Category : Science
ISBN : 9781468453836

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DNA-Ligand Interactions by W. Guschlbauer Pdf

This volume contains the texts of the nineteen lectures presented at the NATO-ASI - FEBS Course on "DNA - ligand interactions: from drugs to proteins." The Advanced Study Institute (ASIl was held from August 30th to September 11th. 1986 in the Abbey of Fontevraud (France). The ASI was attended by 112 participants from a wide scientific horizon and from twentyone different countries. It was in some way a follow-up of the ASI held in Maratea. Italy in May 1981 and which was published in the NATO ASI Life Science series as volume 45. While much has been learned about the way the cellular machinery maintains and transmits the genetic heritage. as well as how these processes are regulated. little is Known about how the interactions between the various partners involved are taKing place. The interactions of drugs and proteins with nucleic acids are of evident importance in the understanding of these problems. The spectacular advances in recombinant DNA technology and the increased sophistication of biophysical techniques. in particular >:-ray diffraction and nuclear magnetic resonance. have created a scientific environment which is highly promising for the future of research in molecular biology. These advances permH the serious hope that biology on the molecular level may become a r-eality. Some of the contributions at the ASI presented the most recent advances in this e>:citing field.

Author : Jost,Saluz
Publisher : Birkhäuser
Page : 319 pages
File Size : 42,9 Mb
Release : 2013-03-13
Category : Science
ISBN : 9783034875615

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A Laboratory Guide to In Vitro Studies of Protein-DNA Interactions by Jost,Saluz Pdf

A Safety Considerations Many techniques described here involve a number of hazards, such as high electrical current and voltage, radioactivity and highly toxic chemicals. It is absolutely essential that the instructions of equipment manufacturers be followed, and that particular attention be paid to the local and federal safety regulations. B Introduction The expression of prokaryotic and eukaryotic genes has been shown most often to be regulated at the level of mRNA synthesis. Thanks to the rapid development of methods for dissecting DNA sequences, cis-acting regulatory elements such as promoters and enhancers have been recognised. More recently, the widely expressed intuition that discrete sequences within these elements constitute binding sites for sequence-specific binding proteins has been confirmed, especially through the use of "footprinting" assays (for examples, Galas and Schmitz, 1978). This and similar assays have already resulted in the recognition, isolation and analysis of DNA-bind ing proteins for several genes. Excellent reviews exist of the structural studies on these transcription regulatory proteins and related DNA elements (for example, Glover, 1989 and Johnson and McKnight, 1989), to which the reader is referred for detailed information. To set the scene for applications of the techniques described in this volume, only the barest outline of previous studies is presented here. Protein-DNA interactions are dependent on very specific tertiary configurations of the binding protein which allow the closest contact with the DNA helix.

Author : Mark C. Williams,L. James Maher, III
Publisher : Springer Science & Business Media
Page : 354 pages
File Size : 50,7 Mb
Release : 2010-10-05
Category : Science
ISBN : 9780387928081

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Biophysics of DNA-Protein Interactions by Mark C. Williams,L. James Maher, III Pdf

Depite the rapid expansion of the field of biophysics, there are very few books that comprehensively treat specific topics in this area. Recently, the field of single molecule biophysics has developed very quickly, and a few books specifically treating single molecule methods are beginning to appear. However, the promise of single molecule biophysics is to contribute to the understanding of specific fields of biology using new methods. This book would focus on the specific topic of the biophysics of DNA-protein interactions, and would include the use of new approaches, including both bulk methods as well as single molecule methods. This would make the book attractive to anyone working in the general area of DNA-protein interactions, which is of course a much wider market than just single molecule biophysicists or even biophysicists. The subject of the book will be the biophysics of DNA-protein interactions, and will include new methods and results that describe the physical mechanism by which proteins interact with DNA. For example, there has been much recent work on the mechanism by which proteins search for specific binding sites on DNA. A few chapters will be devoted to experiments and theory that shed light on this important problem. We will also cover proteins that alter DNA properties to facilitate interactions important for transcription or replication. Another section of the book will cover the biophysical mechanism by which motor proteins interact with DNA. Finally, we will cover larger protein-DNA complexes, such as replication forks, recombination complexes, DNA repair interactions, and their chromatin context.

Author : Timir Tripathi,Vikash Kumar Dubey
Publisher : Academic Press
Page : 716 pages
File Size : 47,8 Mb
Release : 2022-01-14
Category : Science
ISBN : 9780323902656

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Advances in Protein Molecular and Structural Biology Methods by Timir Tripathi,Vikash Kumar Dubey Pdf

Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. Presents a thorough overview of the latest and emerging methods and technologies for protein study Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy Includes computational and machine learning methods Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins

Author : G.D. Fasman
Publisher : Springer Science & Business Media
Page : 739 pages
File Size : 49,5 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781475725087

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Circular Dichroism and the Conformational Analysis of Biomolecules by G.D. Fasman Pdf

''Excellent and very timely....It will undoubtedly become a standard reference for the application of circular dichroism (CD) to biomolecules.'' --- Quarterly Review of Biology, March 1997 ''[T]estament to the book's utility is the fact that during the course of my review I had to 'rescue' it from the desks of graduate students on an almost daily basis. In summary, this is a great book.'' --- American Scientist ''Well documented chapters provide a very good insight into the problems surrounding the conformation of biomacromolecules...An indispensible source of information.'' --- Nahrung, 42(2), 1998 Renowned experts present the first state-of-the-art description of circular dichroism spectroscopy (CD). Chapters present in-depth discussions of the history of the field, the theory of CD for application to globular proteins, membrane proteins, peptides, nucleic acids and their interactions, carbohydrates, and instrumentation. Discussions also feature new techniques using synchrotron radiation, vibrational Raman optical activity, and vibrational CD. More than 250 illustrations supplement the text.

Author : Harald Seitz
Publisher : Springer Science & Business Media
Page : 212 pages
File Size : 45,8 Mb
Release : 2007-01-16
Category : Medical
ISBN : 9783540481478

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Analytics of Protein-DNA Interactions by Harald Seitz Pdf

With contributions by numerous experts

Author : Phoebe A. Rice,Carl C. Correll
Publisher : Unknown
Page : 416 pages
File Size : 50,8 Mb
Release : 2008
Category : Electronic
ISBN : OCLC:1057450960

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Protein-Nucleic Acid Interactions by Phoebe A. Rice,Carl C. Correll Pdf

Author : Biman Bagchi
Publisher : Cambridge University Press
Page : 383 pages
File Size : 49,8 Mb
Release : 2013-11-14
Category : Medical
ISBN : 9781107037298

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Water in Biological and Chemical Processes by Biman Bagchi Pdf

A unified overview of the dynamical properties of water and its unique and diverse role in biological and chemical processes.

Author : Christopher W.J. Smith
Publisher : Oxford University Press, UK
Page : 370 pages
File Size : 49,5 Mb
Release : 1998-07-09
Category : Electronic
ISBN : 9780191591624

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RNA-Protein Interactions : A Practical Approach by Christopher W.J. Smith Pdf

RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Recent research into the role of RNA in cells has elucidated many more vital interactions with proteins. This book provides an up-to-date and comprehensive guide to a wide range of laboratory procedures to investigate the interactions between RNA and proteins. - ;RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field. -

Author : Kiyoshi Nagai,Iain W. Mattaj
Publisher : Oxford University Press, USA
Page : 302 pages
File Size : 50,8 Mb
Release : 1994
Category : Nucleoproteins
ISBN : UOM:39015031725248

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RNA-protein Interactions by Kiyoshi Nagai,Iain W. Mattaj Pdf

The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.